double stranded pbr322 plasmid dna  (New England Biolabs)


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    Name:
    pBR322 Vector
    Description:
    pBR322 Vector 250 ug
    Catalog Number:
    N3033L
    Price:
    302
    Category:
    Vectors Plasmids
    Size:
    250 ug
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    Structured Review

    New England Biolabs double stranded pbr322 plasmid dna
    pBR322 Vector
    pBR322 Vector 250 ug
    https://www.bioz.com/result/double stranded pbr322 plasmid dna/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    double stranded pbr322 plasmid dna - by Bioz Stars, 2021-06
    98/100 stars

    Images

    1) Product Images from "Electrical DNA Sequence Mapping Using Oligodeoxynucleotide Labels and Nanopores"

    Article Title: Electrical DNA Sequence Mapping Using Oligodeoxynucleotide Labels and Nanopores

    Journal: ACS Nano

    doi: 10.1021/acsnano.0c07947

    Sequence-specific labeling of DNA with an oligodeoxynucleotide (ODN). (a) Modification of the DNA at the sequence 5′-TCGA-3′ using M. Taq I and AdoYnODN11 cofactor. The site was covalently labeled with an ODN containing 11 nucleotides (5′-TTATACATCTA-3′). (b) Distribution of the target sequence (5′-TCGA-3′) sites on pBR322 plasmid DNA. (c) Confirmation of the modification using restriction enzymes. The left panel shows the distribution of the sites of the restriction enzymes. The right panel shows the analysis by agarose gel electrophoresis. (d) Linearization of the pBR322 DNA for nanopore measurement. The labeled DNA was linearized with the restriction endonuclease R. Ahd I which cleaves the pBR322 plasmid at a single site.
    Figure Legend Snippet: Sequence-specific labeling of DNA with an oligodeoxynucleotide (ODN). (a) Modification of the DNA at the sequence 5′-TCGA-3′ using M. Taq I and AdoYnODN11 cofactor. The site was covalently labeled with an ODN containing 11 nucleotides (5′-TTATACATCTA-3′). (b) Distribution of the target sequence (5′-TCGA-3′) sites on pBR322 plasmid DNA. (c) Confirmation of the modification using restriction enzymes. The left panel shows the distribution of the sites of the restriction enzymes. The right panel shows the analysis by agarose gel electrophoresis. (d) Linearization of the pBR322 DNA for nanopore measurement. The labeled DNA was linearized with the restriction endonuclease R. Ahd I which cleaves the pBR322 plasmid at a single site.

    Techniques Used: Sequencing, Labeling, Modification, Plasmid Preparation, Agarose Gel Electrophoresis

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Genetic Fusions of Heat-Labile Toxoid (LT) and Heat-Stable Toxin b (STb) of Porcine Enterotoxigenic Escherichia coli Elicit Protective Anti-LT and Anti-STb Antibodies ▿
    Article Snippet: The SOE PCR was performed in a reaction mixture of 1× Pfu DNA polymerase buffer (with Mg2+ ), 200 nM dNTP, 20 μl of each purified PCR product, 1 U Pfu polymerase, and 0.5 U Taq DNA polymerase (Applied Biosystem, Foster City, CA). .. Final PCR products (inserts) and vector pBR322 were digested with NheI and EagI restriction enzymes (New England Biolabs, Ipswich, MA) and ligated with T4 DNA ligase (Invitrogen) under standard conditions ( ). .. Ligated products were introduced into 1836-2 and TOP10 competent cells with standard electroporation ( ).

    Plasmid Preparation:

    Article Title: Genetic Fusions of Heat-Labile Toxoid (LT) and Heat-Stable Toxin b (STb) of Porcine Enterotoxigenic Escherichia coli Elicit Protective Anti-LT and Anti-STb Antibodies ▿
    Article Snippet: The SOE PCR was performed in a reaction mixture of 1× Pfu DNA polymerase buffer (with Mg2+ ), 200 nM dNTP, 20 μl of each purified PCR product, 1 U Pfu polymerase, and 0.5 U Taq DNA polymerase (Applied Biosystem, Foster City, CA). .. Final PCR products (inserts) and vector pBR322 were digested with NheI and EagI restriction enzymes (New England Biolabs, Ipswich, MA) and ligated with T4 DNA ligase (Invitrogen) under standard conditions ( ). .. Ligated products were introduced into 1836-2 and TOP10 competent cells with standard electroporation ( ).

    Article Title: Dataset on the effects of spermidine on linking number differences between histone H1-free and histone H1-bound circular polynucleosomes
    Article Snippet: .. 2.4 Materials Recombinant human histone H2A/H2B dimer, histone H3.1/H4 tetramer, histone H10 , pBR322 vector, Nt.BspQI nicking endonuclease, T4 DNA ligase and Proteinase K were purchased from New England Biolabs Inc. (Singapore). .. QIAquick PCR Purification Kit was obtained from QIAGEN Singapore Pte.

    Article Title: Pyridine and p-Nitrophenyl Oxime Esters with Possible Photochemotherapeutic Activity: Synthesis, DNA Photocleavage and DNA Binding Studies
    Article Snippet: .. The CT DNA concentration was determined by the UV absorbance at 260 nm after 1:20 dilution using ε = 6600 M−1 cm−1 [ ]. pBluescipt KS II plasmid DNA purification was performed using the Nucleospin plasmid kit, according to the protocol provided by the manufacturer (Macherey-Nagel, Duren, Germany). pBR322 was purchased from New England BioLabs (Ipswich, MA, USA).UV-visible (UV-vis) spectra were recorded on a U-2001 dual beam spectrophotometer (Hitachi, Tokyo, Japan). .. Fluorescence emission spectra were recorded in solution on a Hitachi F-7000 fluorescence spectrophotometer (Hitachi, Tokyo, Japan).

    Irradiation:

    Article Title: Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage
    Article Snippet: .. DSB yields were then used as the biological endpoint to calculate VHEE RBE. pBR322 plasmids irradiated in dry and aqueous environments showed little variation in DSB induction over 100–200 MeV, likely due to there being correspondingly little variation in LET (0.220–0.226 keV/μm). ..

    Binding Assay:

    Article Title: A robust assay to measure DNA topology-dependent protein binding affinity
    Article Snippet: .. Tfam binding reactions contained 10 nM Tfam and 10 nM pBR322 in 150 mM NaCl, 10 mM Tris pH 7.5, 10 nM MgCl2 and 1 mM DTT based on the buffer conditions used in ( ). .. Topo IV binding reactions for both the wild type and Y120F constructs contained 2 nM Topo IV and 10 nM pBR322 in 40 mM Tris-HCl pH 7.5, 6 mM MgCl2 , 100 mM potassium acetate, 1 mM DTT and 0.1 mM ethylenediaminetetraacetic acid (EDTA).

    Article Title: A robust assay to measure DNA topology-dependent protein binding affinity
    Article Snippet: .. EcoRV binding reactions contained 2.5 nM EcoRV and 5 nM pBR322 in 100 mM NaCl, 50 mM Tris-HCl pH 7.5 and 10 mM calcium acetate. ..

    Clone Assay:

    Article Title: Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli
    Article Snippet: The square root of ratio of the turbid plaques to clear plaques in Cross 1 to Cross 2 gives the Chi activity. .. Cloning of fusion mutants A 18.3 kb BamH1 fragment containing either wild type rec BCD or rec B-rec D fusion mutants was ligated into the BamHI site of pBR322 by T4 DNA ligase (New England BioLabs), and transformed into V2831 thy - -arg - cells to select thy + -arg + cells. .. Transformants were confirmed by sequencing.

    Transformation Assay:

    Article Title: Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli
    Article Snippet: The square root of ratio of the turbid plaques to clear plaques in Cross 1 to Cross 2 gives the Chi activity. .. Cloning of fusion mutants A 18.3 kb BamH1 fragment containing either wild type rec BCD or rec B-rec D fusion mutants was ligated into the BamHI site of pBR322 by T4 DNA ligase (New England BioLabs), and transformed into V2831 thy - -arg - cells to select thy + -arg + cells. .. Transformants were confirmed by sequencing.

    Recombinant:

    Article Title: Dataset on the effects of spermidine on linking number differences between histone H1-free and histone H1-bound circular polynucleosomes
    Article Snippet: .. 2.4 Materials Recombinant human histone H2A/H2B dimer, histone H3.1/H4 tetramer, histone H10 , pBR322 vector, Nt.BspQI nicking endonuclease, T4 DNA ligase and Proteinase K were purchased from New England Biolabs Inc. (Singapore). .. QIAquick PCR Purification Kit was obtained from QIAGEN Singapore Pte.

    Concentration Assay:

    Article Title: Pyridine and p-Nitrophenyl Oxime Esters with Possible Photochemotherapeutic Activity: Synthesis, DNA Photocleavage and DNA Binding Studies
    Article Snippet: .. The CT DNA concentration was determined by the UV absorbance at 260 nm after 1:20 dilution using ε = 6600 M−1 cm−1 [ ]. pBluescipt KS II plasmid DNA purification was performed using the Nucleospin plasmid kit, according to the protocol provided by the manufacturer (Macherey-Nagel, Duren, Germany). pBR322 was purchased from New England BioLabs (Ipswich, MA, USA).UV-visible (UV-vis) spectra were recorded on a U-2001 dual beam spectrophotometer (Hitachi, Tokyo, Japan). .. Fluorescence emission spectra were recorded in solution on a Hitachi F-7000 fluorescence spectrophotometer (Hitachi, Tokyo, Japan).

    DNA Purification:

    Article Title: Pyridine and p-Nitrophenyl Oxime Esters with Possible Photochemotherapeutic Activity: Synthesis, DNA Photocleavage and DNA Binding Studies
    Article Snippet: .. The CT DNA concentration was determined by the UV absorbance at 260 nm after 1:20 dilution using ε = 6600 M−1 cm−1 [ ]. pBluescipt KS II plasmid DNA purification was performed using the Nucleospin plasmid kit, according to the protocol provided by the manufacturer (Macherey-Nagel, Duren, Germany). pBR322 was purchased from New England BioLabs (Ipswich, MA, USA).UV-visible (UV-vis) spectra were recorded on a U-2001 dual beam spectrophotometer (Hitachi, Tokyo, Japan). .. Fluorescence emission spectra were recorded in solution on a Hitachi F-7000 fluorescence spectrophotometer (Hitachi, Tokyo, Japan).

    Spectrophotometry:

    Article Title: Pyridine and p-Nitrophenyl Oxime Esters with Possible Photochemotherapeutic Activity: Synthesis, DNA Photocleavage and DNA Binding Studies
    Article Snippet: .. The CT DNA concentration was determined by the UV absorbance at 260 nm after 1:20 dilution using ε = 6600 M−1 cm−1 [ ]. pBluescipt KS II plasmid DNA purification was performed using the Nucleospin plasmid kit, according to the protocol provided by the manufacturer (Macherey-Nagel, Duren, Germany). pBR322 was purchased from New England BioLabs (Ipswich, MA, USA).UV-visible (UV-vis) spectra were recorded on a U-2001 dual beam spectrophotometer (Hitachi, Tokyo, Japan). .. Fluorescence emission spectra were recorded in solution on a Hitachi F-7000 fluorescence spectrophotometer (Hitachi, Tokyo, Japan).

    Labeling:

    Article Title: Diabetes and aging alter bone marrow contributions to tissue maintenance
    Article Snippet: However, due to the close proximity of laminin to the satellite cell and myofiber nuclei, we were unable to unambiguously identify pBR322+ nuclei. .. Sections were instead labeled with anti-desmin to identify skeletal muscle followed by pBR322 in situ hybridization, which allowed us to identify pBR322+ nuclei. ..

    In Situ Hybridization:

    Article Title: Diabetes and aging alter bone marrow contributions to tissue maintenance
    Article Snippet: However, due to the close proximity of laminin to the satellite cell and myofiber nuclei, we were unable to unambiguously identify pBR322+ nuclei. .. Sections were instead labeled with anti-desmin to identify skeletal muscle followed by pBR322 in situ hybridization, which allowed us to identify pBR322+ nuclei. ..

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    New England Biolabs double stranded pbr322 plasmid dna
    Sequence-specific labeling of DNA with an oligodeoxynucleotide (ODN). (a) Modification of the DNA at the sequence 5′-TCGA-3′ using M. Taq I and AdoYnODN11 cofactor. The site was covalently labeled with an ODN containing 11 nucleotides (5′-TTATACATCTA-3′). (b) Distribution of the target sequence (5′-TCGA-3′) sites on <t>pBR322</t> plasmid DNA. (c) Confirmation of the modification using restriction enzymes. The left panel shows the distribution of the sites of the restriction enzymes. The right panel shows the analysis by agarose gel electrophoresis. (d) Linearization of the pBR322 DNA for nanopore measurement. The labeled DNA was linearized with the restriction endonuclease R. Ahd I which cleaves the pBR322 plasmid at a single site.
    Double Stranded Pbr322 Plasmid Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/double stranded pbr322 plasmid dna/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    double stranded pbr322 plasmid dna - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier

    93
    New England Biolabs plasmid pbr322 dna
    Activity and expression of endonucleases in PTE cells. ( A ) In the total protein extracts isolated from DNase I WT mice, the strongest endonuclease activity could be obtained when Ca 2+ and Mg 2+ ions were added together, resulting in digested <t>DNA.</t> This is characteristic to DNase I, which therefore provides most of the endonuclease activity in the normal kidney. In the kidney tissue extracts obtained from DNase I KO mice, Mn-dependent endonuclease was the most prominent, suggesting that EndoG is the second major endonuclease in the absence of DNase I (Widlak et al ). Vertical row: O, open circular DNA; L, linear DNA; C, covalently closed circular DNA; D, digested DNA. Horizontal row: control nondigested <t>pBR322</t> DNA; Ca 2+ , 2 mM CaCl 2 , pH 7.5; Mg 2+ , 2 mM MgCl 2 , pH 7.5; CM [Ca 2+ +Mg 2+ ], 2 mM CaCl 2 + 2 mM MgCl 2 ; Mn 2+ , 2 mM MnCl 2 , pH 7.5; E5, 2 mM EDTA, no cations, pH 5 (to measure DNase II activity). ( B ) Expression of endonucleases in WT, EndoG KO, and DNase I KO cells measured using real-time reverse transcriptase polymerase chain reaction. DNase I expression is wiped out almost completely, while EndoG KO is partially inhibited because these cells were isolated from heterozygous animals ( n = 4, * p
    Plasmid Pbr322 Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pbr322 dna/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid pbr322 dna - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Sequence-specific labeling of DNA with an oligodeoxynucleotide (ODN). (a) Modification of the DNA at the sequence 5′-TCGA-3′ using M. Taq I and AdoYnODN11 cofactor. The site was covalently labeled with an ODN containing 11 nucleotides (5′-TTATACATCTA-3′). (b) Distribution of the target sequence (5′-TCGA-3′) sites on pBR322 plasmid DNA. (c) Confirmation of the modification using restriction enzymes. The left panel shows the distribution of the sites of the restriction enzymes. The right panel shows the analysis by agarose gel electrophoresis. (d) Linearization of the pBR322 DNA for nanopore measurement. The labeled DNA was linearized with the restriction endonuclease R. Ahd I which cleaves the pBR322 plasmid at a single site.

    Journal: ACS Nano

    Article Title: Electrical DNA Sequence Mapping Using Oligodeoxynucleotide Labels and Nanopores

    doi: 10.1021/acsnano.0c07947

    Figure Lengend Snippet: Sequence-specific labeling of DNA with an oligodeoxynucleotide (ODN). (a) Modification of the DNA at the sequence 5′-TCGA-3′ using M. Taq I and AdoYnODN11 cofactor. The site was covalently labeled with an ODN containing 11 nucleotides (5′-TTATACATCTA-3′). (b) Distribution of the target sequence (5′-TCGA-3′) sites on pBR322 plasmid DNA. (c) Confirmation of the modification using restriction enzymes. The left panel shows the distribution of the sites of the restriction enzymes. The right panel shows the analysis by agarose gel electrophoresis. (d) Linearization of the pBR322 DNA for nanopore measurement. The labeled DNA was linearized with the restriction endonuclease R. Ahd I which cleaves the pBR322 plasmid at a single site.

    Article Snippet: Sequence-Specific Labeling with ODN ODN-labeled DNA was prepared by incubating double-stranded pBR322 plasmid DNA (100 ng/μL, New England BioLabs (NEB), Ipswich, MA), ODN-modified AdoMet analogue AdoYnODN11 (10 μM) and M.Taq I (2.43 μM, 10 equiv of M.Taq I with respect to 5′-TCGA-3′ recognition sequences on the plasmid) in NEB buffer 4 (110 μL, 20 mM Tris–HCl, 50 mM KOAc, 10 mM Mg(OAc)2 , 1 mM DTT, pH 7.9) at 65 °C for 1 h. Plasmids were purified using the QIAquick PCR purification kit (QIAGEN, Hilden, Germany) according to the instructions of the manufacturer.

    Techniques: Sequencing, Labeling, Modification, Plasmid Preparation, Agarose Gel Electrophoresis

    Activity and expression of endonucleases in PTE cells. ( A ) In the total protein extracts isolated from DNase I WT mice, the strongest endonuclease activity could be obtained when Ca 2+ and Mg 2+ ions were added together, resulting in digested DNA. This is characteristic to DNase I, which therefore provides most of the endonuclease activity in the normal kidney. In the kidney tissue extracts obtained from DNase I KO mice, Mn-dependent endonuclease was the most prominent, suggesting that EndoG is the second major endonuclease in the absence of DNase I (Widlak et al ). Vertical row: O, open circular DNA; L, linear DNA; C, covalently closed circular DNA; D, digested DNA. Horizontal row: control nondigested pBR322 DNA; Ca 2+ , 2 mM CaCl 2 , pH 7.5; Mg 2+ , 2 mM MgCl 2 , pH 7.5; CM [Ca 2+ +Mg 2+ ], 2 mM CaCl 2 + 2 mM MgCl 2 ; Mn 2+ , 2 mM MnCl 2 , pH 7.5; E5, 2 mM EDTA, no cations, pH 5 (to measure DNase II activity). ( B ) Expression of endonucleases in WT, EndoG KO, and DNase I KO cells measured using real-time reverse transcriptase polymerase chain reaction. DNase I expression is wiped out almost completely, while EndoG KO is partially inhibited because these cells were isolated from heterozygous animals ( n = 4, * p

    Journal: DNA and Cell Biology

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases

    doi: 10.1089/dna.2008.0850

    Figure Lengend Snippet: Activity and expression of endonucleases in PTE cells. ( A ) In the total protein extracts isolated from DNase I WT mice, the strongest endonuclease activity could be obtained when Ca 2+ and Mg 2+ ions were added together, resulting in digested DNA. This is characteristic to DNase I, which therefore provides most of the endonuclease activity in the normal kidney. In the kidney tissue extracts obtained from DNase I KO mice, Mn-dependent endonuclease was the most prominent, suggesting that EndoG is the second major endonuclease in the absence of DNase I (Widlak et al ). Vertical row: O, open circular DNA; L, linear DNA; C, covalently closed circular DNA; D, digested DNA. Horizontal row: control nondigested pBR322 DNA; Ca 2+ , 2 mM CaCl 2 , pH 7.5; Mg 2+ , 2 mM MgCl 2 , pH 7.5; CM [Ca 2+ +Mg 2+ ], 2 mM CaCl 2 + 2 mM MgCl 2 ; Mn 2+ , 2 mM MnCl 2 , pH 7.5; E5, 2 mM EDTA, no cations, pH 5 (to measure DNase II activity). ( B ) Expression of endonucleases in WT, EndoG KO, and DNase I KO cells measured using real-time reverse transcriptase polymerase chain reaction. DNase I expression is wiped out almost completely, while EndoG KO is partially inhibited because these cells were isolated from heterozygous animals ( n = 4, * p

    Article Snippet: Endonuclease activity was measured the same way as above in samples containing serially diluted protein (1:5), 1 μg plasmid pBR322 DNA (New England Biolabs), 2 mM CaCl2 , 5 mM MgCl2 , 10 mM Tris-HCl, pH 7.4, and 0.5 mM dithiothreitol to determine the Ca/Mg-dependent (primarily DNase I) endonuclease activity or 5 mM MnCl2 , 10 mM Tris-HCl (pH 7.4), and 0.5 mM dithiothreitol for the Mn-dependent (mainly EndoG) activity.

    Techniques: Activity Assay, Expressing, Isolation, Mouse Assay, Polymerase Chain Reaction

    Endonuclease activity in tubular epithelial cell extract and culture medium. The activity was measured using pBR322 PIA as described in the Materials and Methods section. ( A ) Endonuclease activity is present both in the cellular protein extracts and in the culture medium (left and middle panels). Pretreatment with Lipofectamine does not protect plasmid DNA against in vitro digestion by endonucleases (right panel). Dilutions (1–6) of cell extract or medium 1:1, 1:5, 1:25, 1:125, 1:625, and 1:3125, respectively. O, open circular DNA (with one or more single-strand DNA breaks but no double-strand breaks); L, linear DNA (with one double-strand DNA break); C, covalently closed circular DNA (without DNA breaks), which is the primary substrate for endonucleases. Endonuclease activity is seen only in the first two dilutions in cell extract, and in nondiluted culture medium. ( B ) Endonuclease activity in immortalized TKPTS cells and PTE cells. Primary cells have a higher total endonuclease activity (25 ± 2 units/μg protein in primary cells vs. 7 ± 3 units/μg protein in TKPTS cells, n = 3–6, p

    Journal: DNA and Cell Biology

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases

    doi: 10.1089/dna.2008.0850

    Figure Lengend Snippet: Endonuclease activity in tubular epithelial cell extract and culture medium. The activity was measured using pBR322 PIA as described in the Materials and Methods section. ( A ) Endonuclease activity is present both in the cellular protein extracts and in the culture medium (left and middle panels). Pretreatment with Lipofectamine does not protect plasmid DNA against in vitro digestion by endonucleases (right panel). Dilutions (1–6) of cell extract or medium 1:1, 1:5, 1:25, 1:125, 1:625, and 1:3125, respectively. O, open circular DNA (with one or more single-strand DNA breaks but no double-strand breaks); L, linear DNA (with one double-strand DNA break); C, covalently closed circular DNA (without DNA breaks), which is the primary substrate for endonucleases. Endonuclease activity is seen only in the first two dilutions in cell extract, and in nondiluted culture medium. ( B ) Endonuclease activity in immortalized TKPTS cells and PTE cells. Primary cells have a higher total endonuclease activity (25 ± 2 units/μg protein in primary cells vs. 7 ± 3 units/μg protein in TKPTS cells, n = 3–6, p

    Article Snippet: Endonuclease activity was measured the same way as above in samples containing serially diluted protein (1:5), 1 μg plasmid pBR322 DNA (New England Biolabs), 2 mM CaCl2 , 5 mM MgCl2 , 10 mM Tris-HCl, pH 7.4, and 0.5 mM dithiothreitol to determine the Ca/Mg-dependent (primarily DNase I) endonuclease activity or 5 mM MnCl2 , 10 mM Tris-HCl (pH 7.4), and 0.5 mM dithiothreitol for the Mn-dependent (mainly EndoG) activity.

    Techniques: Activity Assay, Plasmid Preparation, In Vitro

    DNA supercoiling activity of M. tuberculosis WT and A547V GyrB DNA gyrase is sensitive to inhibition by gatifloxacin (GAT) (R, relaxed pBR322; S, supercoiled pBR322). GAT concentrations are expressed in mg/liter. GAT IC 50 s are estimated at 3 mg/liter

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: DNA Gyrase Inhibition Assays Are Necessary To Demonstrate Fluoroquinolone Resistance Secondary to gyrB Mutations in Mycobacterium tuberculosis ▿

    doi: 10.1128/AAC.00707-11

    Figure Lengend Snippet: DNA supercoiling activity of M. tuberculosis WT and A547V GyrB DNA gyrase is sensitive to inhibition by gatifloxacin (GAT) (R, relaxed pBR322; S, supercoiled pBR322). GAT concentrations are expressed in mg/liter. GAT IC 50 s are estimated at 3 mg/liter

    Article Snippet: Supercoiled plasmid pBR322 DNA was purchased from New England BioLabs, and relaxed plasmid pBR322 DNA was from John Innes Enterprises, Ltd.

    Techniques: Activity Assay, Inhibition