Journal: Scientific Reports
Article Title: Central dopamine D 2 receptors regulate plasma glucose levels in mice through autonomic nerves
doi: 10.1038/s41598-020-79292-0
Figure Lengend Snippet: Effects of dopamine D 1 and D 2 receptor agonists and antagonists on plasma glucose levels in mice. ( a ) Effect of SKF 38393 (3 and 10 μg, i.c.v.) on plasma glucose levels. ( b ) Effect of SCH 23390 (1 and 3 μg, i.c.v.) on plasma glucose levels. ( c ) Effect of quinpirole (3 and 10 μg, i.c.v) on plasma glucose levels. ( d ) Effect of l-sulpiride (3 and 10 μg, i.c.v.) on plasma glucose levels. ( e ) Effect of l-sulpiride on hyperglycemia induced by quinpirole. l-Sulpiride (2 μg, i.c.v.) was co-administered with quinpirole (10 μg, i.c.v.). Each point represents the mean ± S.E.M. of 7–16 mice. ** P < 0.01, *** P < 0.001 vs. vehicle group. # P < 0.05, ## P < 0.01 vs. quinpirole group.
Article Snippet: The drugs used were: the dopamine D 1 receptor agonist SKF 38393 hydrochloride (Sigma-Aldrich, St. Louis, MO, USA) ; the dopamine D 1 receptor antagonist R( +)-SCH 23390 hydrochloride (Sigma-Aldrich) ; the dopamine D 2 receptor agonist (-)-quinpirole hydrochloride (Sigma-Aldrich) ; the dopamine D 2 receptor antagonist l-sulpiride (Sigma-Aldrich) , ; the glucocorticoid receptor antagonist RU 486 (Sigma-Aldrich); the β 2 adrenoceptor antagonist ICI 118,551 hydrochloride (Sigma-Aldrich). l-Sulpiride was dissolved in a minimum volume of 0.1 N HCl, neutralized with 0.1 N NaOH to pH 6–7 and adjusted to final volume with saline , ; RU 486 was dissolved in a vehicle containing 90% saline, 5% dimethylsulfoxide (Wako Pure Chemical Industries, Osaka, Japan) and 5% cremophor EL (Sigma-Aldrich); other drugs were dissolved in saline – , .
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