donkey antirabbit igg  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Donkey anti Rabbit IgG H L
    Description:
    Donkey anti Rabbit IgG H L Cross Adsorbed Secondary Antibody for Western Blot IF ICC IHC Flow IP
    Catalog Number:
    sa510049
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
    Buy from Supplier


    Structured Review

    Thermo Fisher donkey antirabbit igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Donkey anti Rabbit IgG H L Cross Adsorbed Secondary Antibody for Western Blot IF ICC IHC Flow IP
    https://www.bioz.com/result/donkey antirabbit igg/product/Thermo Fisher
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    donkey antirabbit igg - by Bioz Stars, 2020-08
    94/100 stars

    Images

    1) Product Images from "Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct"

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct

    Journal: Experimental Biology and Medicine

    doi: 10.1177/1535370216673746

    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Figure Legend Snippet: Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)

    Techniques Used: Fluorescence, Staining, Negative Control

    Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)
    Figure Legend Snippet: Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)

    Techniques Used: Fluorescence, Staining, Cell Culture, Incubation, Negative Control

    2) Product Images from "Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct"

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct

    Journal: Experimental Biology and Medicine

    doi: 10.1177/1535370216673746

    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Figure Legend Snippet: Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)

    Techniques Used: Fluorescence, Staining, Negative Control

    Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)
    Figure Legend Snippet: Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)

    Techniques Used: Fluorescence, Staining, Cell Culture, Incubation, Negative Control

    Related Articles

    Staining:

    Article Title: Accelerated Aging of Intervertebral Discs in a Mouse Model of Progeria
    Article Snippet: .. After washing, the sections were incubated for 60 min in a mixture of fluorescent-labeled secondary antibody (Alexa Fluor 488 conjugated donkey anti-rabbit IgG(H + L)) and Alexa Fluor 647 Phalloidin1 to stain cytoplasmic actin, then washed and mounted in Prolong Gold anti-fade reagent with DAPI (Invitrogen, Carlsbad, CA, ) to stain for nuclei. .. Quantitative fluorescent confocal microscopy ( fluoview1000 ) was used to measure the number and distribution of a variety of fluorescently labeled cells and extracellular matrix molecules.

    Article Title: Olfactory Sensory Neurons Control Dendritic Complexity of Mitral Cells via Notch Signaling
    Article Snippet: .. Secondary goat and donkey antibodies (Thermo Fischer Scientific) were used to visualize the signals for sections: dendrite analyses, Alexa Fluor 546-conjugated anti-rat IgG; EYFP/Tbx21 staining, Alexa Fluor 488-conjugated anti-rat IgG and Alexa Fluor 633-conjugated anti-mouse IgG; EYFP/NICD1/Tbx21 staining, Alexa Fluor 488-conjugated anti-rat IgG, Alexa Fluor 633-conjugated anti-rabbit IgG and Alexa Fluor 546-conjugated anti-mouse IgG; Tbr1 staining, Alexa Fluor 546-conjugated anti-rabbit IgG, and for the coverslips: Alexa Fluor 488-conjugated anti-goat IgG, Alexa Fluor 594-conjugated anti-mouse IgG and Alexa Fluor 350-conjugated anti-rabbit IgG, respectively. .. Image analysis and quantification Large views of the brains were obtained with a fluorescence stereomicroscope (MZ16F, Leica).

    Incubation:

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct
    Article Snippet: .. Following washing with PBS, the slices were incubated with secondary antibodies of donkey antigoat IgG (Alexa Fluor® 594-conjugated; Invitrogen), and donkey antirabbit IgG (Alexa Fluor® 488-conjugated; Invitrogen), and goat antimouse IgG (Alexa Fluor® 594-conjugated; Invitrogen) for 30 min at room temperature in the humidified chamber. .. After nucleus counterstain and mounting, the slices were evaluated by a fluorescence microscope (Leica DM4000B).

    Article Title: Accelerated Aging of Intervertebral Discs in a Mouse Model of Progeria
    Article Snippet: .. After washing, the sections were incubated for 60 min in a mixture of fluorescent-labeled secondary antibody (Alexa Fluor 488 conjugated donkey anti-rabbit IgG(H + L)) and Alexa Fluor 647 Phalloidin1 to stain cytoplasmic actin, then washed and mounted in Prolong Gold anti-fade reagent with DAPI (Invitrogen, Carlsbad, CA, ) to stain for nuclei. .. Quantitative fluorescent confocal microscopy ( fluoview1000 ) was used to measure the number and distribution of a variety of fluorescently labeled cells and extracellular matrix molecules.

    Article Title: Inner Mitochondrial Translocase Tim50 Is Central in Adrenal and Testicular Steroid Synthesis
    Article Snippet: .. After blocking with 3% nonfat dry milk for 45 min, the membranes were probed overnight with the primary antibodies at 4°C and then incubated with the peroxide-conjugated goat anti-rabbit IgG, anti-mouse IgG, or anti-donkey IgG (Pierce, Rockford, IL) as needed. .. Signals were developed with a chemiluminescent reagent (Pierce) and developed on a X-ray film.

    Article Title: Regulation of Cyclin E by transcription factors of the naïve pluripotency network in mouse embryonic stem cells
    Article Snippet: .. After three rinses (10 min each) with TBS, the cells were incubated with fluorochrome-conjugated secondary antibody [Alexa Fluor 488-conjugated donkey anti-rabbit IgG [H + L], (1/500 dilution), Life Technologies, A21206; Alexa Fluor 647-conjugated donkey anti-mouse IgG [H + L], (1/400 dilution), Life Technologies, A31571] at room temperature for 1 h. The cells were examined under confocal imaging (DM 6000 CS SP5; Leica). ..

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct
    Article Snippet: .. The cells were then incubated with a mixed solution of donkey antigoat IgG (Alexa Fluor® 594-conjugated; Invitrogen, Grand Island, NY) and donkey antirabbit IgG (Alexa Fluor® 488-conjugated; Invitrogen) for 60 min in the dark. .. Next, a mounting medium for fluorescence with DAPI (Vectashield, Burlingame, CA) was used to counterstain the nucleus.

    Imaging:

    Article Title: Regulation of Cyclin E by transcription factors of the naïve pluripotency network in mouse embryonic stem cells
    Article Snippet: .. After three rinses (10 min each) with TBS, the cells were incubated with fluorochrome-conjugated secondary antibody [Alexa Fluor 488-conjugated donkey anti-rabbit IgG [H + L], (1/500 dilution), Life Technologies, A21206; Alexa Fluor 647-conjugated donkey anti-mouse IgG [H + L], (1/400 dilution), Life Technologies, A31571] at room temperature for 1 h. The cells were examined under confocal imaging (DM 6000 CS SP5; Leica). ..

    Blocking Assay:

    Article Title: Inner Mitochondrial Translocase Tim50 Is Central in Adrenal and Testicular Steroid Synthesis
    Article Snippet: .. After blocking with 3% nonfat dry milk for 45 min, the membranes were probed overnight with the primary antibodies at 4°C and then incubated with the peroxide-conjugated goat anti-rabbit IgG, anti-mouse IgG, or anti-donkey IgG (Pierce, Rockford, IL) as needed. .. Signals were developed with a chemiluminescent reagent (Pierce) and developed on a X-ray film.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Thermo Fisher dylight 549 conjugated donkey antirabbit igg
    Dylight 549 Conjugated Donkey Antirabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dylight 549 conjugated donkey antirabbit igg/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dylight 549 conjugated donkey antirabbit igg - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    99
    Thermo Fisher donkey anti rabbit igg h l highly cross adsorbed secondary antibody
    Donkey Anti Rabbit Igg H L Highly Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey anti rabbit igg h l highly cross adsorbed secondary antibody/product/Thermo Fisher
    Average 99 stars, based on 237 article reviews
    Price from $9.99 to $1999.99
    donkey anti rabbit igg h l highly cross adsorbed secondary antibody - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Thermo Fisher donkey antirabbit igg alexa fluor 488
    Donkey Antirabbit Igg Alexa Fluor 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey antirabbit igg alexa fluor 488/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey antirabbit igg alexa fluor 488 - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results