donkey anti rat igg h l secondary antibody biotin pre absorbed  (Novus Biologicals)

 
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    Name:
    Donkey anti Rat IgG H L Secondary Antibody Biotin Pre absorbed
    Description:
    The Donkey anti Rat IgG H L Secondary Antibody Biotin Pre absorbed from Novus Biologicals is a donkey polyclonal antibody to IgG H L This antibody reacts with rat The Donkey anti Rat IgG H L Secondary Antibody Biotin Pre absorbed has been validated for the following applications Western Blot ELISA Immunohistochemistry Immunohistochemistry Paraffin
    Catalog Number:
    NBP1-75379
    Price:
    199
    Category:
    Secondary Antibodies
    Purity:
    Immunogen affinity purified
    Conjugate:
    Biotin
    Immunogen:
    Purified Rat IgG (H&L)
    Size:
    2 mg
    Host:
    Donkey
    Isotype:
    IgG
    Buy from Supplier


    Structured Review

    Novus Biologicals donkey anti rat igg h l secondary antibody biotin pre absorbed
    Donkey anti Rat IgG H L Secondary Antibody Biotin Pre absorbed
    The Donkey anti Rat IgG H L Secondary Antibody Biotin Pre absorbed from Novus Biologicals is a donkey polyclonal antibody to IgG H L This antibody reacts with rat The Donkey anti Rat IgG H L Secondary Antibody Biotin Pre absorbed has been validated for the following applications Western Blot ELISA Immunohistochemistry Immunohistochemistry Paraffin
    https://www.bioz.com/result/donkey anti rat igg h l secondary antibody biotin pre absorbed/product/Novus Biologicals
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey anti rat igg h l secondary antibody biotin pre absorbed - by Bioz Stars, 2021-07
    93/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Iron nanoparticle-labeled murine mesenchymal stromal cells in an osteoarthritic model persists and suggests anti-inflammatory mechanism of action
    Article Snippet: Sections were then treated with Proteinase K (20 μg/mL) for 15 minutes for antigen retrieval and blocked with DAKO background reducing protein blocking agent (Agilent, X090930-2) prior to 1 hour incubation of anti-CD206 antibody (1:200, R & D systems, AF2535, ), anti-F4/80 antibody (1:100, Serotec, ), anti-iNOS antibody (1:100, Abcam, ab15323), Prussian Blue (equal volumes of 20% HCl in dH20 and 10% K4 Fe(CN)6 -3H2 O mixture, Sigma Aldrich respectively) and anti-Sca-1 antibody (1:100, Bio-Rad, MCA2782, ) at room temperature to detect homeostatic macrophages, total macrophages, proinflammatory macrophages, ferumoxytol-labeled MSCs and total cells respectively. .. Detection was performed using anti-goat secondary antibody (for CD206, 1:1000 Novus Biologicals, NB120-7124, ), anti-rat secondary antibody (for F4/80 and Sca-1, 1:500 Novus Biologicals, NBP1-75379, ) or biotinylated anti-rabbit secondary antibody (for cleaved caspase-3, 1:2000 or for iNOS, 1:200, Vector Labs BA-1000, ) for 30 minutes, followed by avidin-biotin complex detection system (VECTASTAIN Elite ABC system, Vector Labs PK-6100) for 30 minutes and DAB-horseradish peroxidase substrate detection system. .. Cells were counterstained with hematoxylin (Vector Labs, H3404) to detect total cells.

    Recombinant:

    Article Title: Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d RESOURCE AVAILABILITY B Lead contact B Materials availability B Data and code availability d EXPERIMENTAL MODEL AND SUBJECT DETAILS B Mice B Parasites B Sand flies, mosquitoes, and ticks rearing d METHOD DETAILS B Sand fly infection B Salivary gland homogenate B Mice exposure to vector bites or needle pokes B Immunoblotting and ELISA immunosorbent assays B Immunohistochemistry and confocal microscopy B SnPPIX and CORM-2 treatment B Cytokine determination by quantitative RT-PCR B Flow cytometry analysis B Lesion measurements B Parasite load by quantitative real-time-PCR d QUANTIFICATION AND STATISTICAL ANALYSIS B Statistical analysis SUPPLEMENTAL INFORMATION Supplemental Information can be found online at https://doi.org/10.1016/j. celrep.2020.108317. .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-HO-1 abcam Cat#ab13243; RRID: AB_299790 Rabbit anti-HSP90 Cell Signaling Technology Cat#4877S; RRID: AB_2233307 Goat anti-rabbit secondary antibody conjugated to horseradish peroxidase Cell Signaling Technology Cat#7074S; RRID: AB_2099233 Goat anti-IBA-1 abcam Cat#ab107159; RRID: AB_10972670 Rabbit anti-myeloperoxidase abcam Cat#ab9535; RRID: AB_307322 Rat anti-mouse TER-119 (clone TER-119) BD Biosciences Cat#550565; RRID: AB_393756 AF488 Donkey anti-rabbit Thermo-Fisher Scientific Cat#A-21206; RRID: AB_2535792 AF594 Donkey anti-mouse Thermo-Fisher Scientific Cat#A-21203; RRID: AB_141633 AF680 Donkey anti-goat Thermo-Fisher Scientific Cat#A-32860; RRID: AB_2762841 Biotinylated Donkey anti-rat Novus Biologicals Cat#NBP1-75379; RRID: AB_11027475 Streptavidin AF750 Thermo-Fisher Scientific Cat#S21384; Temporary PRID: AB_2876869 TruStain FcX anti-mouse CD16/32 (clone 93) Biolegend Cat#101320; RRID: AB_1574975 PE-Cy7 anti-mouse/human CD11b (clone M1/70) Biolegend Cat#101216; RRID: AB_312799 FITC anti-mouse Ly6C (clone HK1.4) Biolegend Cat#128006; RRID: AB_1186135 PE anti-mouse Ly6G (clone 1A8) Biolegend Cat#127608; RRID: AB_1186099 PerCP anti-mouse F4/80 (clone BM8) Biolegend Cat#123126; RRID: AB_893483 PE anti-mouse CD163/M130 (clone TNKUPJ) Bioss Antibodies Cat#bs-2527R-PE; RRID: AB_11062797 PE anti-mouse CD91 (clone EPR3724) abcam Cat#ab225060; Temporary PRID: AB_2876868 APC anti-mouse IL1-b pro-form antibody (clone NJTEN3) eBioscience Cat#17-7114-80; RRID: AB_10670739 APC anti-mouse TER-119 (clone TER-119) Biolegend Cat#116212; RRID: AB_313713 Chemicals, Peptides, and Recombinant Proteins Low Phytoestrogen Rodent Diet Teklad global-Envigo Cat#2919 Liberase TL Research Grade Roche Cat#5401020001 Tin Protoporphyrin IX dichloride (SnPPIX) Enzo Life Science Cat#ALX-430-051-M025 Tricarbonyldichlororuthenium (II) dimer (CORM-2) Sigma-Aldrich Cat#288144 Pierce RIPA Lysis and Extraction Buffer Thermo-Fisher Scientific Cat#89901 Phenylmethylsulfonyl Fluoride solution Sigma-Aldrich Cat#93482 Halt Protease Inhibitor Cocktail Thermo-Fisher Scientific Cat#87786 Peanut Agglutinin (PNA), Unconjugated Vector Laboratories Cat#L-1070 Critical Commercial Assays Pierce BCA Protein Assay Kit Thermo-Fisher Scientific Cat#23227 Mouse Heme Oxygenase 1 ELISA Kit abcam Cat#ab204524 Mouse Hemoglobin ELISA Kit abcam Cat#ab157715 Flash Western Chemiluminescence Azure Biosystems Cat#AC221 PureLink RNA Mini kit Invitrogen -Thermo-Fisher Scientific Cat#12183020 cDNA reverse transcription kit Applied Biosystems - Thermo-Fisher Scientific Cat#4368813 (Continued on next page) e1 Cell Reports 33, 108317, October 27, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER TaqMan gene expression master mix Applied Biosystems - Thermo-Fisher Scientific Cat#4369016 High Pure PCR Template Preparation Kit Roche Cat#11796828001 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit Thermo-Fisher Scientific Cat#L34959 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 Experimental Models: Cell Lines Leishmania major WR 2885 strain LMVR/NIAID/NIH Originally isolated from a US soldier deployed to Iraq N/A Experimental Models: Organisms/Strains Mouse: C57BL/6 Charles River Laboratories Strain Code #027 Mouse: C57BL/6 R26CreERT2 (B6.129-Gt (ROSA)26Sortm1 (cre/ERT2)Tyj/J) The Jackson Laboratory Cat#008463 Mouse: Hmox1fl/fl Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: R26CreERT2/CreERT2Hmoxfl/fl (Hmox1R26fl/fl) Instituto Gulbenkian de Ciência: (Ramos et al., 2019) N/A Mouse: C57BL/6 Hmox1R26D/D Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: Hmox1lox/lox Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Lutzomyia longipalpis (Jacobina colony) LMVR/NIAID/NIH N/A Phebotomus duboscqi (Mali colony) LMVR/NIAID/NIH N/A Aedes aegypti (Liverpool strain) LMVR/NIAID/NIH N/A Anopheles gambie (G3 strain) LMVR/NIAID/NIH N/A Ornithodorus turicata (Florida strain) LMVR/NIAID/NIH N/A Oligonucleotides Leishmania forward primer JW11 (50- CCTATT TTACACCAA CCCCCAGT-30) (Dey et al., 2018) N/A Leishmania reverse primer JW12 (50-GGGTAGGGGCGT TCTGCGAAA-30) (Dey et al., 2018) N/A Leishmania TaqMan probe (50- [Aminoc6+TxRed] RA AARKKVRTRCAGAAAYCC CGT [BHQ2]-30) (Dey et al., 2018) N/A Software and Algorithms FlowJo 10 Software FlowJO, LLC https://www.flowjo.com/solutions/flowjo/ downloads ImageJ.exe 1.52a Software NIH https://imagej.nih.gov/ij/download.html Prism 8 Software GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Imaris x64 9.5.0 Software Oxford Instruments https://imaris.oxinst.com/ microscopy-imaging-software-free-trial EndNote X9 Software Web of Science Group https://endnote.com/downloads/ Other Schneider’s Drosophila Medium GIBCO Cat#21720001 Heat Inactivated Fetal Bovine Serum GIBCO Cat#16140-071 Penicillin/Streptomycin GIBCO Cat#15140122 Rabbit defibrinated blood Spring Valley Laboratories N/A (Continued on next page) Cell Reports 33, 108317, October 27, 2020 e2.

    Lysis:

    Article Title: Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d RESOURCE AVAILABILITY B Lead contact B Materials availability B Data and code availability d EXPERIMENTAL MODEL AND SUBJECT DETAILS B Mice B Parasites B Sand flies, mosquitoes, and ticks rearing d METHOD DETAILS B Sand fly infection B Salivary gland homogenate B Mice exposure to vector bites or needle pokes B Immunoblotting and ELISA immunosorbent assays B Immunohistochemistry and confocal microscopy B SnPPIX and CORM-2 treatment B Cytokine determination by quantitative RT-PCR B Flow cytometry analysis B Lesion measurements B Parasite load by quantitative real-time-PCR d QUANTIFICATION AND STATISTICAL ANALYSIS B Statistical analysis SUPPLEMENTAL INFORMATION Supplemental Information can be found online at https://doi.org/10.1016/j. celrep.2020.108317. .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-HO-1 abcam Cat#ab13243; RRID: AB_299790 Rabbit anti-HSP90 Cell Signaling Technology Cat#4877S; RRID: AB_2233307 Goat anti-rabbit secondary antibody conjugated to horseradish peroxidase Cell Signaling Technology Cat#7074S; RRID: AB_2099233 Goat anti-IBA-1 abcam Cat#ab107159; RRID: AB_10972670 Rabbit anti-myeloperoxidase abcam Cat#ab9535; RRID: AB_307322 Rat anti-mouse TER-119 (clone TER-119) BD Biosciences Cat#550565; RRID: AB_393756 AF488 Donkey anti-rabbit Thermo-Fisher Scientific Cat#A-21206; RRID: AB_2535792 AF594 Donkey anti-mouse Thermo-Fisher Scientific Cat#A-21203; RRID: AB_141633 AF680 Donkey anti-goat Thermo-Fisher Scientific Cat#A-32860; RRID: AB_2762841 Biotinylated Donkey anti-rat Novus Biologicals Cat#NBP1-75379; RRID: AB_11027475 Streptavidin AF750 Thermo-Fisher Scientific Cat#S21384; Temporary PRID: AB_2876869 TruStain FcX anti-mouse CD16/32 (clone 93) Biolegend Cat#101320; RRID: AB_1574975 PE-Cy7 anti-mouse/human CD11b (clone M1/70) Biolegend Cat#101216; RRID: AB_312799 FITC anti-mouse Ly6C (clone HK1.4) Biolegend Cat#128006; RRID: AB_1186135 PE anti-mouse Ly6G (clone 1A8) Biolegend Cat#127608; RRID: AB_1186099 PerCP anti-mouse F4/80 (clone BM8) Biolegend Cat#123126; RRID: AB_893483 PE anti-mouse CD163/M130 (clone TNKUPJ) Bioss Antibodies Cat#bs-2527R-PE; RRID: AB_11062797 PE anti-mouse CD91 (clone EPR3724) abcam Cat#ab225060; Temporary PRID: AB_2876868 APC anti-mouse IL1-b pro-form antibody (clone NJTEN3) eBioscience Cat#17-7114-80; RRID: AB_10670739 APC anti-mouse TER-119 (clone TER-119) Biolegend Cat#116212; RRID: AB_313713 Chemicals, Peptides, and Recombinant Proteins Low Phytoestrogen Rodent Diet Teklad global-Envigo Cat#2919 Liberase TL Research Grade Roche Cat#5401020001 Tin Protoporphyrin IX dichloride (SnPPIX) Enzo Life Science Cat#ALX-430-051-M025 Tricarbonyldichlororuthenium (II) dimer (CORM-2) Sigma-Aldrich Cat#288144 Pierce RIPA Lysis and Extraction Buffer Thermo-Fisher Scientific Cat#89901 Phenylmethylsulfonyl Fluoride solution Sigma-Aldrich Cat#93482 Halt Protease Inhibitor Cocktail Thermo-Fisher Scientific Cat#87786 Peanut Agglutinin (PNA), Unconjugated Vector Laboratories Cat#L-1070 Critical Commercial Assays Pierce BCA Protein Assay Kit Thermo-Fisher Scientific Cat#23227 Mouse Heme Oxygenase 1 ELISA Kit abcam Cat#ab204524 Mouse Hemoglobin ELISA Kit abcam Cat#ab157715 Flash Western Chemiluminescence Azure Biosystems Cat#AC221 PureLink RNA Mini kit Invitrogen -Thermo-Fisher Scientific Cat#12183020 cDNA reverse transcription kit Applied Biosystems - Thermo-Fisher Scientific Cat#4368813 (Continued on next page) e1 Cell Reports 33, 108317, October 27, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER TaqMan gene expression master mix Applied Biosystems - Thermo-Fisher Scientific Cat#4369016 High Pure PCR Template Preparation Kit Roche Cat#11796828001 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit Thermo-Fisher Scientific Cat#L34959 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 Experimental Models: Cell Lines Leishmania major WR 2885 strain LMVR/NIAID/NIH Originally isolated from a US soldier deployed to Iraq N/A Experimental Models: Organisms/Strains Mouse: C57BL/6 Charles River Laboratories Strain Code #027 Mouse: C57BL/6 R26CreERT2 (B6.129-Gt (ROSA)26Sortm1 (cre/ERT2)Tyj/J) The Jackson Laboratory Cat#008463 Mouse: Hmox1fl/fl Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: R26CreERT2/CreERT2Hmoxfl/fl (Hmox1R26fl/fl) Instituto Gulbenkian de Ciência: (Ramos et al., 2019) N/A Mouse: C57BL/6 Hmox1R26D/D Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: Hmox1lox/lox Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Lutzomyia longipalpis (Jacobina colony) LMVR/NIAID/NIH N/A Phebotomus duboscqi (Mali colony) LMVR/NIAID/NIH N/A Aedes aegypti (Liverpool strain) LMVR/NIAID/NIH N/A Anopheles gambie (G3 strain) LMVR/NIAID/NIH N/A Ornithodorus turicata (Florida strain) LMVR/NIAID/NIH N/A Oligonucleotides Leishmania forward primer JW11 (50- CCTATT TTACACCAA CCCCCAGT-30) (Dey et al., 2018) N/A Leishmania reverse primer JW12 (50-GGGTAGGGGCGT TCTGCGAAA-30) (Dey et al., 2018) N/A Leishmania TaqMan probe (50- [Aminoc6+TxRed] RA AARKKVRTRCAGAAAYCC CGT [BHQ2]-30) (Dey et al., 2018) N/A Software and Algorithms FlowJo 10 Software FlowJO, LLC https://www.flowjo.com/solutions/flowjo/ downloads ImageJ.exe 1.52a Software NIH https://imagej.nih.gov/ij/download.html Prism 8 Software GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Imaris x64 9.5.0 Software Oxford Instruments https://imaris.oxinst.com/ microscopy-imaging-software-free-trial EndNote X9 Software Web of Science Group https://endnote.com/downloads/ Other Schneider’s Drosophila Medium GIBCO Cat#21720001 Heat Inactivated Fetal Bovine Serum GIBCO Cat#16140-071 Penicillin/Streptomycin GIBCO Cat#15140122 Rabbit defibrinated blood Spring Valley Laboratories N/A (Continued on next page) Cell Reports 33, 108317, October 27, 2020 e2.

    Protease Inhibitor:

    Article Title: Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d RESOURCE AVAILABILITY B Lead contact B Materials availability B Data and code availability d EXPERIMENTAL MODEL AND SUBJECT DETAILS B Mice B Parasites B Sand flies, mosquitoes, and ticks rearing d METHOD DETAILS B Sand fly infection B Salivary gland homogenate B Mice exposure to vector bites or needle pokes B Immunoblotting and ELISA immunosorbent assays B Immunohistochemistry and confocal microscopy B SnPPIX and CORM-2 treatment B Cytokine determination by quantitative RT-PCR B Flow cytometry analysis B Lesion measurements B Parasite load by quantitative real-time-PCR d QUANTIFICATION AND STATISTICAL ANALYSIS B Statistical analysis SUPPLEMENTAL INFORMATION Supplemental Information can be found online at https://doi.org/10.1016/j. celrep.2020.108317. .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-HO-1 abcam Cat#ab13243; RRID: AB_299790 Rabbit anti-HSP90 Cell Signaling Technology Cat#4877S; RRID: AB_2233307 Goat anti-rabbit secondary antibody conjugated to horseradish peroxidase Cell Signaling Technology Cat#7074S; RRID: AB_2099233 Goat anti-IBA-1 abcam Cat#ab107159; RRID: AB_10972670 Rabbit anti-myeloperoxidase abcam Cat#ab9535; RRID: AB_307322 Rat anti-mouse TER-119 (clone TER-119) BD Biosciences Cat#550565; RRID: AB_393756 AF488 Donkey anti-rabbit Thermo-Fisher Scientific Cat#A-21206; RRID: AB_2535792 AF594 Donkey anti-mouse Thermo-Fisher Scientific Cat#A-21203; RRID: AB_141633 AF680 Donkey anti-goat Thermo-Fisher Scientific Cat#A-32860; RRID: AB_2762841 Biotinylated Donkey anti-rat Novus Biologicals Cat#NBP1-75379; RRID: AB_11027475 Streptavidin AF750 Thermo-Fisher Scientific Cat#S21384; Temporary PRID: AB_2876869 TruStain FcX anti-mouse CD16/32 (clone 93) Biolegend Cat#101320; RRID: AB_1574975 PE-Cy7 anti-mouse/human CD11b (clone M1/70) Biolegend Cat#101216; RRID: AB_312799 FITC anti-mouse Ly6C (clone HK1.4) Biolegend Cat#128006; RRID: AB_1186135 PE anti-mouse Ly6G (clone 1A8) Biolegend Cat#127608; RRID: AB_1186099 PerCP anti-mouse F4/80 (clone BM8) Biolegend Cat#123126; RRID: AB_893483 PE anti-mouse CD163/M130 (clone TNKUPJ) Bioss Antibodies Cat#bs-2527R-PE; RRID: AB_11062797 PE anti-mouse CD91 (clone EPR3724) abcam Cat#ab225060; Temporary PRID: AB_2876868 APC anti-mouse IL1-b pro-form antibody (clone NJTEN3) eBioscience Cat#17-7114-80; RRID: AB_10670739 APC anti-mouse TER-119 (clone TER-119) Biolegend Cat#116212; RRID: AB_313713 Chemicals, Peptides, and Recombinant Proteins Low Phytoestrogen Rodent Diet Teklad global-Envigo Cat#2919 Liberase TL Research Grade Roche Cat#5401020001 Tin Protoporphyrin IX dichloride (SnPPIX) Enzo Life Science Cat#ALX-430-051-M025 Tricarbonyldichlororuthenium (II) dimer (CORM-2) Sigma-Aldrich Cat#288144 Pierce RIPA Lysis and Extraction Buffer Thermo-Fisher Scientific Cat#89901 Phenylmethylsulfonyl Fluoride solution Sigma-Aldrich Cat#93482 Halt Protease Inhibitor Cocktail Thermo-Fisher Scientific Cat#87786 Peanut Agglutinin (PNA), Unconjugated Vector Laboratories Cat#L-1070 Critical Commercial Assays Pierce BCA Protein Assay Kit Thermo-Fisher Scientific Cat#23227 Mouse Heme Oxygenase 1 ELISA Kit abcam Cat#ab204524 Mouse Hemoglobin ELISA Kit abcam Cat#ab157715 Flash Western Chemiluminescence Azure Biosystems Cat#AC221 PureLink RNA Mini kit Invitrogen -Thermo-Fisher Scientific Cat#12183020 cDNA reverse transcription kit Applied Biosystems - Thermo-Fisher Scientific Cat#4368813 (Continued on next page) e1 Cell Reports 33, 108317, October 27, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER TaqMan gene expression master mix Applied Biosystems - Thermo-Fisher Scientific Cat#4369016 High Pure PCR Template Preparation Kit Roche Cat#11796828001 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit Thermo-Fisher Scientific Cat#L34959 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 Experimental Models: Cell Lines Leishmania major WR 2885 strain LMVR/NIAID/NIH Originally isolated from a US soldier deployed to Iraq N/A Experimental Models: Organisms/Strains Mouse: C57BL/6 Charles River Laboratories Strain Code #027 Mouse: C57BL/6 R26CreERT2 (B6.129-Gt (ROSA)26Sortm1 (cre/ERT2)Tyj/J) The Jackson Laboratory Cat#008463 Mouse: Hmox1fl/fl Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: R26CreERT2/CreERT2Hmoxfl/fl (Hmox1R26fl/fl) Instituto Gulbenkian de Ciência: (Ramos et al., 2019) N/A Mouse: C57BL/6 Hmox1R26D/D Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: Hmox1lox/lox Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Lutzomyia longipalpis (Jacobina colony) LMVR/NIAID/NIH N/A Phebotomus duboscqi (Mali colony) LMVR/NIAID/NIH N/A Aedes aegypti (Liverpool strain) LMVR/NIAID/NIH N/A Anopheles gambie (G3 strain) LMVR/NIAID/NIH N/A Ornithodorus turicata (Florida strain) LMVR/NIAID/NIH N/A Oligonucleotides Leishmania forward primer JW11 (50- CCTATT TTACACCAA CCCCCAGT-30) (Dey et al., 2018) N/A Leishmania reverse primer JW12 (50-GGGTAGGGGCGT TCTGCGAAA-30) (Dey et al., 2018) N/A Leishmania TaqMan probe (50- [Aminoc6+TxRed] RA AARKKVRTRCAGAAAYCC CGT [BHQ2]-30) (Dey et al., 2018) N/A Software and Algorithms FlowJo 10 Software FlowJO, LLC https://www.flowjo.com/solutions/flowjo/ downloads ImageJ.exe 1.52a Software NIH https://imagej.nih.gov/ij/download.html Prism 8 Software GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Imaris x64 9.5.0 Software Oxford Instruments https://imaris.oxinst.com/ microscopy-imaging-software-free-trial EndNote X9 Software Web of Science Group https://endnote.com/downloads/ Other Schneider’s Drosophila Medium GIBCO Cat#21720001 Heat Inactivated Fetal Bovine Serum GIBCO Cat#16140-071 Penicillin/Streptomycin GIBCO Cat#15140122 Rabbit defibrinated blood Spring Valley Laboratories N/A (Continued on next page) Cell Reports 33, 108317, October 27, 2020 e2.

    Bicinchoninic Acid Protein Assay:

    Article Title: Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d RESOURCE AVAILABILITY B Lead contact B Materials availability B Data and code availability d EXPERIMENTAL MODEL AND SUBJECT DETAILS B Mice B Parasites B Sand flies, mosquitoes, and ticks rearing d METHOD DETAILS B Sand fly infection B Salivary gland homogenate B Mice exposure to vector bites or needle pokes B Immunoblotting and ELISA immunosorbent assays B Immunohistochemistry and confocal microscopy B SnPPIX and CORM-2 treatment B Cytokine determination by quantitative RT-PCR B Flow cytometry analysis B Lesion measurements B Parasite load by quantitative real-time-PCR d QUANTIFICATION AND STATISTICAL ANALYSIS B Statistical analysis SUPPLEMENTAL INFORMATION Supplemental Information can be found online at https://doi.org/10.1016/j. celrep.2020.108317. .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-HO-1 abcam Cat#ab13243; RRID: AB_299790 Rabbit anti-HSP90 Cell Signaling Technology Cat#4877S; RRID: AB_2233307 Goat anti-rabbit secondary antibody conjugated to horseradish peroxidase Cell Signaling Technology Cat#7074S; RRID: AB_2099233 Goat anti-IBA-1 abcam Cat#ab107159; RRID: AB_10972670 Rabbit anti-myeloperoxidase abcam Cat#ab9535; RRID: AB_307322 Rat anti-mouse TER-119 (clone TER-119) BD Biosciences Cat#550565; RRID: AB_393756 AF488 Donkey anti-rabbit Thermo-Fisher Scientific Cat#A-21206; RRID: AB_2535792 AF594 Donkey anti-mouse Thermo-Fisher Scientific Cat#A-21203; RRID: AB_141633 AF680 Donkey anti-goat Thermo-Fisher Scientific Cat#A-32860; RRID: AB_2762841 Biotinylated Donkey anti-rat Novus Biologicals Cat#NBP1-75379; RRID: AB_11027475 Streptavidin AF750 Thermo-Fisher Scientific Cat#S21384; Temporary PRID: AB_2876869 TruStain FcX anti-mouse CD16/32 (clone 93) Biolegend Cat#101320; RRID: AB_1574975 PE-Cy7 anti-mouse/human CD11b (clone M1/70) Biolegend Cat#101216; RRID: AB_312799 FITC anti-mouse Ly6C (clone HK1.4) Biolegend Cat#128006; RRID: AB_1186135 PE anti-mouse Ly6G (clone 1A8) Biolegend Cat#127608; RRID: AB_1186099 PerCP anti-mouse F4/80 (clone BM8) Biolegend Cat#123126; RRID: AB_893483 PE anti-mouse CD163/M130 (clone TNKUPJ) Bioss Antibodies Cat#bs-2527R-PE; RRID: AB_11062797 PE anti-mouse CD91 (clone EPR3724) abcam Cat#ab225060; Temporary PRID: AB_2876868 APC anti-mouse IL1-b pro-form antibody (clone NJTEN3) eBioscience Cat#17-7114-80; RRID: AB_10670739 APC anti-mouse TER-119 (clone TER-119) Biolegend Cat#116212; RRID: AB_313713 Chemicals, Peptides, and Recombinant Proteins Low Phytoestrogen Rodent Diet Teklad global-Envigo Cat#2919 Liberase TL Research Grade Roche Cat#5401020001 Tin Protoporphyrin IX dichloride (SnPPIX) Enzo Life Science Cat#ALX-430-051-M025 Tricarbonyldichlororuthenium (II) dimer (CORM-2) Sigma-Aldrich Cat#288144 Pierce RIPA Lysis and Extraction Buffer Thermo-Fisher Scientific Cat#89901 Phenylmethylsulfonyl Fluoride solution Sigma-Aldrich Cat#93482 Halt Protease Inhibitor Cocktail Thermo-Fisher Scientific Cat#87786 Peanut Agglutinin (PNA), Unconjugated Vector Laboratories Cat#L-1070 Critical Commercial Assays Pierce BCA Protein Assay Kit Thermo-Fisher Scientific Cat#23227 Mouse Heme Oxygenase 1 ELISA Kit abcam Cat#ab204524 Mouse Hemoglobin ELISA Kit abcam Cat#ab157715 Flash Western Chemiluminescence Azure Biosystems Cat#AC221 PureLink RNA Mini kit Invitrogen -Thermo-Fisher Scientific Cat#12183020 cDNA reverse transcription kit Applied Biosystems - Thermo-Fisher Scientific Cat#4368813 (Continued on next page) e1 Cell Reports 33, 108317, October 27, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER TaqMan gene expression master mix Applied Biosystems - Thermo-Fisher Scientific Cat#4369016 High Pure PCR Template Preparation Kit Roche Cat#11796828001 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit Thermo-Fisher Scientific Cat#L34959 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 Experimental Models: Cell Lines Leishmania major WR 2885 strain LMVR/NIAID/NIH Originally isolated from a US soldier deployed to Iraq N/A Experimental Models: Organisms/Strains Mouse: C57BL/6 Charles River Laboratories Strain Code #027 Mouse: C57BL/6 R26CreERT2 (B6.129-Gt (ROSA)26Sortm1 (cre/ERT2)Tyj/J) The Jackson Laboratory Cat#008463 Mouse: Hmox1fl/fl Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: R26CreERT2/CreERT2Hmoxfl/fl (Hmox1R26fl/fl) Instituto Gulbenkian de Ciência: (Ramos et al., 2019) N/A Mouse: C57BL/6 Hmox1R26D/D Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: Hmox1lox/lox Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Lutzomyia longipalpis (Jacobina colony) LMVR/NIAID/NIH N/A Phebotomus duboscqi (Mali colony) LMVR/NIAID/NIH N/A Aedes aegypti (Liverpool strain) LMVR/NIAID/NIH N/A Anopheles gambie (G3 strain) LMVR/NIAID/NIH N/A Ornithodorus turicata (Florida strain) LMVR/NIAID/NIH N/A Oligonucleotides Leishmania forward primer JW11 (50- CCTATT TTACACCAA CCCCCAGT-30) (Dey et al., 2018) N/A Leishmania reverse primer JW12 (50-GGGTAGGGGCGT TCTGCGAAA-30) (Dey et al., 2018) N/A Leishmania TaqMan probe (50- [Aminoc6+TxRed] RA AARKKVRTRCAGAAAYCC CGT [BHQ2]-30) (Dey et al., 2018) N/A Software and Algorithms FlowJo 10 Software FlowJO, LLC https://www.flowjo.com/solutions/flowjo/ downloads ImageJ.exe 1.52a Software NIH https://imagej.nih.gov/ij/download.html Prism 8 Software GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Imaris x64 9.5.0 Software Oxford Instruments https://imaris.oxinst.com/ microscopy-imaging-software-free-trial EndNote X9 Software Web of Science Group https://endnote.com/downloads/ Other Schneider’s Drosophila Medium GIBCO Cat#21720001 Heat Inactivated Fetal Bovine Serum GIBCO Cat#16140-071 Penicillin/Streptomycin GIBCO Cat#15140122 Rabbit defibrinated blood Spring Valley Laboratories N/A (Continued on next page) Cell Reports 33, 108317, October 27, 2020 e2.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d RESOURCE AVAILABILITY B Lead contact B Materials availability B Data and code availability d EXPERIMENTAL MODEL AND SUBJECT DETAILS B Mice B Parasites B Sand flies, mosquitoes, and ticks rearing d METHOD DETAILS B Sand fly infection B Salivary gland homogenate B Mice exposure to vector bites or needle pokes B Immunoblotting and ELISA immunosorbent assays B Immunohistochemistry and confocal microscopy B SnPPIX and CORM-2 treatment B Cytokine determination by quantitative RT-PCR B Flow cytometry analysis B Lesion measurements B Parasite load by quantitative real-time-PCR d QUANTIFICATION AND STATISTICAL ANALYSIS B Statistical analysis SUPPLEMENTAL INFORMATION Supplemental Information can be found online at https://doi.org/10.1016/j. celrep.2020.108317. .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-HO-1 abcam Cat#ab13243; RRID: AB_299790 Rabbit anti-HSP90 Cell Signaling Technology Cat#4877S; RRID: AB_2233307 Goat anti-rabbit secondary antibody conjugated to horseradish peroxidase Cell Signaling Technology Cat#7074S; RRID: AB_2099233 Goat anti-IBA-1 abcam Cat#ab107159; RRID: AB_10972670 Rabbit anti-myeloperoxidase abcam Cat#ab9535; RRID: AB_307322 Rat anti-mouse TER-119 (clone TER-119) BD Biosciences Cat#550565; RRID: AB_393756 AF488 Donkey anti-rabbit Thermo-Fisher Scientific Cat#A-21206; RRID: AB_2535792 AF594 Donkey anti-mouse Thermo-Fisher Scientific Cat#A-21203; RRID: AB_141633 AF680 Donkey anti-goat Thermo-Fisher Scientific Cat#A-32860; RRID: AB_2762841 Biotinylated Donkey anti-rat Novus Biologicals Cat#NBP1-75379; RRID: AB_11027475 Streptavidin AF750 Thermo-Fisher Scientific Cat#S21384; Temporary PRID: AB_2876869 TruStain FcX anti-mouse CD16/32 (clone 93) Biolegend Cat#101320; RRID: AB_1574975 PE-Cy7 anti-mouse/human CD11b (clone M1/70) Biolegend Cat#101216; RRID: AB_312799 FITC anti-mouse Ly6C (clone HK1.4) Biolegend Cat#128006; RRID: AB_1186135 PE anti-mouse Ly6G (clone 1A8) Biolegend Cat#127608; RRID: AB_1186099 PerCP anti-mouse F4/80 (clone BM8) Biolegend Cat#123126; RRID: AB_893483 PE anti-mouse CD163/M130 (clone TNKUPJ) Bioss Antibodies Cat#bs-2527R-PE; RRID: AB_11062797 PE anti-mouse CD91 (clone EPR3724) abcam Cat#ab225060; Temporary PRID: AB_2876868 APC anti-mouse IL1-b pro-form antibody (clone NJTEN3) eBioscience Cat#17-7114-80; RRID: AB_10670739 APC anti-mouse TER-119 (clone TER-119) Biolegend Cat#116212; RRID: AB_313713 Chemicals, Peptides, and Recombinant Proteins Low Phytoestrogen Rodent Diet Teklad global-Envigo Cat#2919 Liberase TL Research Grade Roche Cat#5401020001 Tin Protoporphyrin IX dichloride (SnPPIX) Enzo Life Science Cat#ALX-430-051-M025 Tricarbonyldichlororuthenium (II) dimer (CORM-2) Sigma-Aldrich Cat#288144 Pierce RIPA Lysis and Extraction Buffer Thermo-Fisher Scientific Cat#89901 Phenylmethylsulfonyl Fluoride solution Sigma-Aldrich Cat#93482 Halt Protease Inhibitor Cocktail Thermo-Fisher Scientific Cat#87786 Peanut Agglutinin (PNA), Unconjugated Vector Laboratories Cat#L-1070 Critical Commercial Assays Pierce BCA Protein Assay Kit Thermo-Fisher Scientific Cat#23227 Mouse Heme Oxygenase 1 ELISA Kit abcam Cat#ab204524 Mouse Hemoglobin ELISA Kit abcam Cat#ab157715 Flash Western Chemiluminescence Azure Biosystems Cat#AC221 PureLink RNA Mini kit Invitrogen -Thermo-Fisher Scientific Cat#12183020 cDNA reverse transcription kit Applied Biosystems - Thermo-Fisher Scientific Cat#4368813 (Continued on next page) e1 Cell Reports 33, 108317, October 27, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER TaqMan gene expression master mix Applied Biosystems - Thermo-Fisher Scientific Cat#4369016 High Pure PCR Template Preparation Kit Roche Cat#11796828001 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit Thermo-Fisher Scientific Cat#L34959 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 Experimental Models: Cell Lines Leishmania major WR 2885 strain LMVR/NIAID/NIH Originally isolated from a US soldier deployed to Iraq N/A Experimental Models: Organisms/Strains Mouse: C57BL/6 Charles River Laboratories Strain Code #027 Mouse: C57BL/6 R26CreERT2 (B6.129-Gt (ROSA)26Sortm1 (cre/ERT2)Tyj/J) The Jackson Laboratory Cat#008463 Mouse: Hmox1fl/fl Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: R26CreERT2/CreERT2Hmoxfl/fl (Hmox1R26fl/fl) Instituto Gulbenkian de Ciência: (Ramos et al., 2019) N/A Mouse: C57BL/6 Hmox1R26D/D Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: Hmox1lox/lox Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Lutzomyia longipalpis (Jacobina colony) LMVR/NIAID/NIH N/A Phebotomus duboscqi (Mali colony) LMVR/NIAID/NIH N/A Aedes aegypti (Liverpool strain) LMVR/NIAID/NIH N/A Anopheles gambie (G3 strain) LMVR/NIAID/NIH N/A Ornithodorus turicata (Florida strain) LMVR/NIAID/NIH N/A Oligonucleotides Leishmania forward primer JW11 (50- CCTATT TTACACCAA CCCCCAGT-30) (Dey et al., 2018) N/A Leishmania reverse primer JW12 (50-GGGTAGGGGCGT TCTGCGAAA-30) (Dey et al., 2018) N/A Leishmania TaqMan probe (50- [Aminoc6+TxRed] RA AARKKVRTRCAGAAAYCC CGT [BHQ2]-30) (Dey et al., 2018) N/A Software and Algorithms FlowJo 10 Software FlowJO, LLC https://www.flowjo.com/solutions/flowjo/ downloads ImageJ.exe 1.52a Software NIH https://imagej.nih.gov/ij/download.html Prism 8 Software GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Imaris x64 9.5.0 Software Oxford Instruments https://imaris.oxinst.com/ microscopy-imaging-software-free-trial EndNote X9 Software Web of Science Group https://endnote.com/downloads/ Other Schneider’s Drosophila Medium GIBCO Cat#21720001 Heat Inactivated Fetal Bovine Serum GIBCO Cat#16140-071 Penicillin/Streptomycin GIBCO Cat#15140122 Rabbit defibrinated blood Spring Valley Laboratories N/A (Continued on next page) Cell Reports 33, 108317, October 27, 2020 e2.

    Western Blot:

    Article Title: Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d RESOURCE AVAILABILITY B Lead contact B Materials availability B Data and code availability d EXPERIMENTAL MODEL AND SUBJECT DETAILS B Mice B Parasites B Sand flies, mosquitoes, and ticks rearing d METHOD DETAILS B Sand fly infection B Salivary gland homogenate B Mice exposure to vector bites or needle pokes B Immunoblotting and ELISA immunosorbent assays B Immunohistochemistry and confocal microscopy B SnPPIX and CORM-2 treatment B Cytokine determination by quantitative RT-PCR B Flow cytometry analysis B Lesion measurements B Parasite load by quantitative real-time-PCR d QUANTIFICATION AND STATISTICAL ANALYSIS B Statistical analysis SUPPLEMENTAL INFORMATION Supplemental Information can be found online at https://doi.org/10.1016/j. celrep.2020.108317. .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-HO-1 abcam Cat#ab13243; RRID: AB_299790 Rabbit anti-HSP90 Cell Signaling Technology Cat#4877S; RRID: AB_2233307 Goat anti-rabbit secondary antibody conjugated to horseradish peroxidase Cell Signaling Technology Cat#7074S; RRID: AB_2099233 Goat anti-IBA-1 abcam Cat#ab107159; RRID: AB_10972670 Rabbit anti-myeloperoxidase abcam Cat#ab9535; RRID: AB_307322 Rat anti-mouse TER-119 (clone TER-119) BD Biosciences Cat#550565; RRID: AB_393756 AF488 Donkey anti-rabbit Thermo-Fisher Scientific Cat#A-21206; RRID: AB_2535792 AF594 Donkey anti-mouse Thermo-Fisher Scientific Cat#A-21203; RRID: AB_141633 AF680 Donkey anti-goat Thermo-Fisher Scientific Cat#A-32860; RRID: AB_2762841 Biotinylated Donkey anti-rat Novus Biologicals Cat#NBP1-75379; RRID: AB_11027475 Streptavidin AF750 Thermo-Fisher Scientific Cat#S21384; Temporary PRID: AB_2876869 TruStain FcX anti-mouse CD16/32 (clone 93) Biolegend Cat#101320; RRID: AB_1574975 PE-Cy7 anti-mouse/human CD11b (clone M1/70) Biolegend Cat#101216; RRID: AB_312799 FITC anti-mouse Ly6C (clone HK1.4) Biolegend Cat#128006; RRID: AB_1186135 PE anti-mouse Ly6G (clone 1A8) Biolegend Cat#127608; RRID: AB_1186099 PerCP anti-mouse F4/80 (clone BM8) Biolegend Cat#123126; RRID: AB_893483 PE anti-mouse CD163/M130 (clone TNKUPJ) Bioss Antibodies Cat#bs-2527R-PE; RRID: AB_11062797 PE anti-mouse CD91 (clone EPR3724) abcam Cat#ab225060; Temporary PRID: AB_2876868 APC anti-mouse IL1-b pro-form antibody (clone NJTEN3) eBioscience Cat#17-7114-80; RRID: AB_10670739 APC anti-mouse TER-119 (clone TER-119) Biolegend Cat#116212; RRID: AB_313713 Chemicals, Peptides, and Recombinant Proteins Low Phytoestrogen Rodent Diet Teklad global-Envigo Cat#2919 Liberase TL Research Grade Roche Cat#5401020001 Tin Protoporphyrin IX dichloride (SnPPIX) Enzo Life Science Cat#ALX-430-051-M025 Tricarbonyldichlororuthenium (II) dimer (CORM-2) Sigma-Aldrich Cat#288144 Pierce RIPA Lysis and Extraction Buffer Thermo-Fisher Scientific Cat#89901 Phenylmethylsulfonyl Fluoride solution Sigma-Aldrich Cat#93482 Halt Protease Inhibitor Cocktail Thermo-Fisher Scientific Cat#87786 Peanut Agglutinin (PNA), Unconjugated Vector Laboratories Cat#L-1070 Critical Commercial Assays Pierce BCA Protein Assay Kit Thermo-Fisher Scientific Cat#23227 Mouse Heme Oxygenase 1 ELISA Kit abcam Cat#ab204524 Mouse Hemoglobin ELISA Kit abcam Cat#ab157715 Flash Western Chemiluminescence Azure Biosystems Cat#AC221 PureLink RNA Mini kit Invitrogen -Thermo-Fisher Scientific Cat#12183020 cDNA reverse transcription kit Applied Biosystems - Thermo-Fisher Scientific Cat#4368813 (Continued on next page) e1 Cell Reports 33, 108317, October 27, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER TaqMan gene expression master mix Applied Biosystems - Thermo-Fisher Scientific Cat#4369016 High Pure PCR Template Preparation Kit Roche Cat#11796828001 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit Thermo-Fisher Scientific Cat#L34959 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 Experimental Models: Cell Lines Leishmania major WR 2885 strain LMVR/NIAID/NIH Originally isolated from a US soldier deployed to Iraq N/A Experimental Models: Organisms/Strains Mouse: C57BL/6 Charles River Laboratories Strain Code #027 Mouse: C57BL/6 R26CreERT2 (B6.129-Gt (ROSA)26Sortm1 (cre/ERT2)Tyj/J) The Jackson Laboratory Cat#008463 Mouse: Hmox1fl/fl Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: R26CreERT2/CreERT2Hmoxfl/fl (Hmox1R26fl/fl) Instituto Gulbenkian de Ciência: (Ramos et al., 2019) N/A Mouse: C57BL/6 Hmox1R26D/D Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: Hmox1lox/lox Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Lutzomyia longipalpis (Jacobina colony) LMVR/NIAID/NIH N/A Phebotomus duboscqi (Mali colony) LMVR/NIAID/NIH N/A Aedes aegypti (Liverpool strain) LMVR/NIAID/NIH N/A Anopheles gambie (G3 strain) LMVR/NIAID/NIH N/A Ornithodorus turicata (Florida strain) LMVR/NIAID/NIH N/A Oligonucleotides Leishmania forward primer JW11 (50- CCTATT TTACACCAA CCCCCAGT-30) (Dey et al., 2018) N/A Leishmania reverse primer JW12 (50-GGGTAGGGGCGT TCTGCGAAA-30) (Dey et al., 2018) N/A Leishmania TaqMan probe (50- [Aminoc6+TxRed] RA AARKKVRTRCAGAAAYCC CGT [BHQ2]-30) (Dey et al., 2018) N/A Software and Algorithms FlowJo 10 Software FlowJO, LLC https://www.flowjo.com/solutions/flowjo/ downloads ImageJ.exe 1.52a Software NIH https://imagej.nih.gov/ij/download.html Prism 8 Software GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Imaris x64 9.5.0 Software Oxford Instruments https://imaris.oxinst.com/ microscopy-imaging-software-free-trial EndNote X9 Software Web of Science Group https://endnote.com/downloads/ Other Schneider’s Drosophila Medium GIBCO Cat#21720001 Heat Inactivated Fetal Bovine Serum GIBCO Cat#16140-071 Penicillin/Streptomycin GIBCO Cat#15140122 Rabbit defibrinated blood Spring Valley Laboratories N/A (Continued on next page) Cell Reports 33, 108317, October 27, 2020 e2.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d RESOURCE AVAILABILITY B Lead contact B Materials availability B Data and code availability d EXPERIMENTAL MODEL AND SUBJECT DETAILS B Mice B Parasites B Sand flies, mosquitoes, and ticks rearing d METHOD DETAILS B Sand fly infection B Salivary gland homogenate B Mice exposure to vector bites or needle pokes B Immunoblotting and ELISA immunosorbent assays B Immunohistochemistry and confocal microscopy B SnPPIX and CORM-2 treatment B Cytokine determination by quantitative RT-PCR B Flow cytometry analysis B Lesion measurements B Parasite load by quantitative real-time-PCR d QUANTIFICATION AND STATISTICAL ANALYSIS B Statistical analysis SUPPLEMENTAL INFORMATION Supplemental Information can be found online at https://doi.org/10.1016/j. celrep.2020.108317. .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-HO-1 abcam Cat#ab13243; RRID: AB_299790 Rabbit anti-HSP90 Cell Signaling Technology Cat#4877S; RRID: AB_2233307 Goat anti-rabbit secondary antibody conjugated to horseradish peroxidase Cell Signaling Technology Cat#7074S; RRID: AB_2099233 Goat anti-IBA-1 abcam Cat#ab107159; RRID: AB_10972670 Rabbit anti-myeloperoxidase abcam Cat#ab9535; RRID: AB_307322 Rat anti-mouse TER-119 (clone TER-119) BD Biosciences Cat#550565; RRID: AB_393756 AF488 Donkey anti-rabbit Thermo-Fisher Scientific Cat#A-21206; RRID: AB_2535792 AF594 Donkey anti-mouse Thermo-Fisher Scientific Cat#A-21203; RRID: AB_141633 AF680 Donkey anti-goat Thermo-Fisher Scientific Cat#A-32860; RRID: AB_2762841 Biotinylated Donkey anti-rat Novus Biologicals Cat#NBP1-75379; RRID: AB_11027475 Streptavidin AF750 Thermo-Fisher Scientific Cat#S21384; Temporary PRID: AB_2876869 TruStain FcX anti-mouse CD16/32 (clone 93) Biolegend Cat#101320; RRID: AB_1574975 PE-Cy7 anti-mouse/human CD11b (clone M1/70) Biolegend Cat#101216; RRID: AB_312799 FITC anti-mouse Ly6C (clone HK1.4) Biolegend Cat#128006; RRID: AB_1186135 PE anti-mouse Ly6G (clone 1A8) Biolegend Cat#127608; RRID: AB_1186099 PerCP anti-mouse F4/80 (clone BM8) Biolegend Cat#123126; RRID: AB_893483 PE anti-mouse CD163/M130 (clone TNKUPJ) Bioss Antibodies Cat#bs-2527R-PE; RRID: AB_11062797 PE anti-mouse CD91 (clone EPR3724) abcam Cat#ab225060; Temporary PRID: AB_2876868 APC anti-mouse IL1-b pro-form antibody (clone NJTEN3) eBioscience Cat#17-7114-80; RRID: AB_10670739 APC anti-mouse TER-119 (clone TER-119) Biolegend Cat#116212; RRID: AB_313713 Chemicals, Peptides, and Recombinant Proteins Low Phytoestrogen Rodent Diet Teklad global-Envigo Cat#2919 Liberase TL Research Grade Roche Cat#5401020001 Tin Protoporphyrin IX dichloride (SnPPIX) Enzo Life Science Cat#ALX-430-051-M025 Tricarbonyldichlororuthenium (II) dimer (CORM-2) Sigma-Aldrich Cat#288144 Pierce RIPA Lysis and Extraction Buffer Thermo-Fisher Scientific Cat#89901 Phenylmethylsulfonyl Fluoride solution Sigma-Aldrich Cat#93482 Halt Protease Inhibitor Cocktail Thermo-Fisher Scientific Cat#87786 Peanut Agglutinin (PNA), Unconjugated Vector Laboratories Cat#L-1070 Critical Commercial Assays Pierce BCA Protein Assay Kit Thermo-Fisher Scientific Cat#23227 Mouse Heme Oxygenase 1 ELISA Kit abcam Cat#ab204524 Mouse Hemoglobin ELISA Kit abcam Cat#ab157715 Flash Western Chemiluminescence Azure Biosystems Cat#AC221 PureLink RNA Mini kit Invitrogen -Thermo-Fisher Scientific Cat#12183020 cDNA reverse transcription kit Applied Biosystems - Thermo-Fisher Scientific Cat#4368813 (Continued on next page) e1 Cell Reports 33, 108317, October 27, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER TaqMan gene expression master mix Applied Biosystems - Thermo-Fisher Scientific Cat#4369016 High Pure PCR Template Preparation Kit Roche Cat#11796828001 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit Thermo-Fisher Scientific Cat#L34959 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 Experimental Models: Cell Lines Leishmania major WR 2885 strain LMVR/NIAID/NIH Originally isolated from a US soldier deployed to Iraq N/A Experimental Models: Organisms/Strains Mouse: C57BL/6 Charles River Laboratories Strain Code #027 Mouse: C57BL/6 R26CreERT2 (B6.129-Gt (ROSA)26Sortm1 (cre/ERT2)Tyj/J) The Jackson Laboratory Cat#008463 Mouse: Hmox1fl/fl Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: R26CreERT2/CreERT2Hmoxfl/fl (Hmox1R26fl/fl) Instituto Gulbenkian de Ciência: (Ramos et al., 2019) N/A Mouse: C57BL/6 Hmox1R26D/D Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Mouse: Hmox1lox/lox Instituto Gulbenkian de Ciência; (Ramos et al., 2019) N/A Lutzomyia longipalpis (Jacobina colony) LMVR/NIAID/NIH N/A Phebotomus duboscqi (Mali colony) LMVR/NIAID/NIH N/A Aedes aegypti (Liverpool strain) LMVR/NIAID/NIH N/A Anopheles gambie (G3 strain) LMVR/NIAID/NIH N/A Ornithodorus turicata (Florida strain) LMVR/NIAID/NIH N/A Oligonucleotides Leishmania forward primer JW11 (50- CCTATT TTACACCAA CCCCCAGT-30) (Dey et al., 2018) N/A Leishmania reverse primer JW12 (50-GGGTAGGGGCGT TCTGCGAAA-30) (Dey et al., 2018) N/A Leishmania TaqMan probe (50- [Aminoc6+TxRed] RA AARKKVRTRCAGAAAYCC CGT [BHQ2]-30) (Dey et al., 2018) N/A Software and Algorithms FlowJo 10 Software FlowJO, LLC https://www.flowjo.com/solutions/flowjo/ downloads ImageJ.exe 1.52a Software NIH https://imagej.nih.gov/ij/download.html Prism 8 Software GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Imaris x64 9.5.0 Software Oxford Instruments https://imaris.oxinst.com/ microscopy-imaging-software-free-trial EndNote X9 Software Web of Science Group https://endnote.com/downloads/ Other Schneider’s Drosophila Medium GIBCO Cat#21720001 Heat Inactivated Fetal Bovine Serum GIBCO Cat#16140-071 Penicillin/Streptomycin GIBCO Cat#15140122 Rabbit defibrinated blood Spring Valley Laboratories N/A (Continued on next page) Cell Reports 33, 108317, October 27, 2020 e2.

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