Structured Review

GE Healthcare donkey anti goat igg
<t>Defensin</t> α-1 inhibits T. cruzi trypomastigote motility. Trypomastigotes were incubated with several concentrations of defensin α-1 alone or in the presence of defensin α-1 preincubated with anti-defensin α-1 <t>IgG</t> or preimmune
Donkey Anti Goat Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/donkey anti goat igg/product/GE Healthcare
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
donkey anti goat igg - by Bioz Stars, 2020-07
92/100 stars

Images

1) Product Images from "Cellular Response to Trypanosoma cruzi Infection Induces Secretion of Defensin ?-1, Which Damages the Flagellum, Neutralizes Trypanosome Motility, and Inhibits Infection"

Article Title: Cellular Response to Trypanosoma cruzi Infection Induces Secretion of Defensin ?-1, Which Damages the Flagellum, Neutralizes Trypanosome Motility, and Inhibits Infection

Journal: Infection and Immunity

doi: 10.1128/IAI.01459-12

Defensin α-1 inhibits T. cruzi trypomastigote motility. Trypomastigotes were incubated with several concentrations of defensin α-1 alone or in the presence of defensin α-1 preincubated with anti-defensin α-1 IgG or preimmune
Figure Legend Snippet: Defensin α-1 inhibits T. cruzi trypomastigote motility. Trypomastigotes were incubated with several concentrations of defensin α-1 alone or in the presence of defensin α-1 preincubated with anti-defensin α-1 IgG or preimmune

Techniques Used: Incubation

Defensin α-1 binds to the flagellum of invasive trypomastigotes. (A) Trypomastigotes were incubated with defensin α-1 for 2 min followed by anti-defensin α-1 IgG, developed with protein A linked to gold particles, and observed
Figure Legend Snippet: Defensin α-1 binds to the flagellum of invasive trypomastigotes. (A) Trypomastigotes were incubated with defensin α-1 for 2 min followed by anti-defensin α-1 IgG, developed with protein A linked to gold particles, and observed

Techniques Used: Incubation

Related Articles

Isolation:

Article Title: A significant portion of mitochondrial proton leak in intact thymocytes depends on expression of UCP2
Article Snippet: .. For detection of UCP2 protein in mitochondria isolated from thymocytes, immunoblotting was performed by using 50 μg of mitochondrial protein, goat anti-UCP2 IgG [UCP2(C-20):sc6525, Santa Cruz Biotechnology] as the first antibody (1:100) and donkey anti-goat IgG (PA42005, Amersham Pharmacia) as the second antibody (1:2,000). ..

Incubation:

Article Title: Expression of Nuclear Receptor Coactivators in the Human Fetal Membranes at Term before and after Labor
Article Snippet: .. Membranes were washed with 1 × PBS-T four times for 5 min and then incubated with secondary antibody diluted 1 : 2000 in blocking solution at room temperature for 1 h. Horseradish peroxidase-linked donkey anti-rabbit IgG (Amersham Biosciences, Buckinghamshire, UK) was used as a secondary antibody for all the reactions except for Brm, where donkey anti-goat IgG (Amersham Biosciences) was used. .. The filters were washed with 1 × PBS-T four times for 5 min, developed using Western Lightning Chemiluminescence Reagent Plus Kit (Perkin Elmer, Boston, MA), and exposed to Kodak Biomax MR films (Eastman Kodak, Rochester, NY, USA) for a sufficient time.

Article Title: Cellular Response to Trypanosoma cruzi Infection Induces Secretion of Defensin ?-1, Which Damages the Flagellum, Neutralizes Trypanosome Motility, and Inhibits Infection
Article Snippet: .. Samples were incubated with anti-defensin α-1 IgG or preimmune IgG and probed with donkey anti-goat IgG conjugated with 12-nm gold particles (Amersham Bioscience, Piscataway, NJ). .. Samples were stained with phosphotungstic acid and observed with a Phillips CM-12 electron microscope.

Article Title: Active Plasma Kallikrein Localizes to Mast Cells and Regulates Epithelial Cell Apoptosis, Adipocyte Differentiation, and Stromal Remodeling during Mammary Gland Involution *
Article Snippet: .. Membranes were washed and incubated with donkey anti-goat IgG (1:2000, Amersham Biosciences) before detection with ECL chemiluminescent reagent (Amersham Biosciences). .. Histology, Immunohistochemistry, and Immunofluorescence —Right no. 4 mammary glands were removed, weighed, and immediately embedded in OCT (Sakura, Torrance, CA) medium on dry ice.

Autoradiography:

Article Title: Mapping the zinc transporting system in mammary cells: Molecular analysis reveals a phenotype-dependent zinc transporting network during lactation
Article Snippet: .. Proteins were detected with donkey, anti-rabbit IgG, donkey anti-goat IgG or donkey anti-chicken IgY conjugated to horseradish peroxidase (Amersham Pharmacia Biotech), visualized with Super Signal Femto Chemiluminescent Detection System (Pierce, Rockford, IL) and exposed to autoradiography film. .. Relative band density was quantified using the Carestream Gel Logic 212 Pro.

Blocking Assay:

Article Title: Expression of Nuclear Receptor Coactivators in the Human Fetal Membranes at Term before and after Labor
Article Snippet: .. Membranes were washed with 1 × PBS-T four times for 5 min and then incubated with secondary antibody diluted 1 : 2000 in blocking solution at room temperature for 1 h. Horseradish peroxidase-linked donkey anti-rabbit IgG (Amersham Biosciences, Buckinghamshire, UK) was used as a secondary antibody for all the reactions except for Brm, where donkey anti-goat IgG (Amersham Biosciences) was used. .. The filters were washed with 1 × PBS-T four times for 5 min, developed using Western Lightning Chemiluminescence Reagent Plus Kit (Perkin Elmer, Boston, MA), and exposed to Kodak Biomax MR films (Eastman Kodak, Rochester, NY, USA) for a sufficient time.

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    GE Healthcare peroxidase conjugated donkey anti goat igg antibody
    Peroxidase Conjugated Donkey Anti Goat Igg Antibody, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare peroxidase conjugated donkey anti rabbit igg
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    GE Healthcare horseradish peroxidase conjugated goat donkey anti rabbit igg
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