dog osteosarcoma d17  (ATCC)

Journal: Oncology Letters

Article Title: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

doi: 10.3892/ol.2016.5400

Figure Lengend Snippet: Oct4 siRNA reduces Oct4 expression in F5M2 cells. F5M2 cells were treated with Oct4 siRNA or scramble for 3 days. (A) mRNA and (B) protein expression levels were measured by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively (n=6). *P<0.05, **P<0.01 vs. the scramble group. Oct4, octamer-binding transcription factor 4; siRNA, small interfering RNA.

Article Snippet: SOSP-9607, MG63 and F5M2 osteosarcoma cell lines and the hFOB1.19 osteoblast cell line were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay, Small Interfering RNA

Journal: Oncology Letters

Article Title: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

doi: 10.3892/ol.2016.5400

Figure Lengend Snippet: Oct4 downregulation decreases proliferation and invasion ability of F5M2 cells. F5M2 cells were treated with of Oct4 siRNA1 or scramble for 3 days. (A) Proliferation was measured by MTT assay, (B) cell apoptosis was assayed using Annexin V by flow cytometry, and (C) invasion ability was evaluated by Transwell assay. Magnification, ×200. (D) Quantified data of invasion has been presented (n=6). *P<0.01 vs. the control. Oct4, octamer-binding transcription factor 4; siRNA, small interfering RNA; FITC, fluorescien isothiocyanate; PI, propidium iodide.

Article Snippet: SOSP-9607, MG63 and F5M2 osteosarcoma cell lines and the hFOB1.19 osteoblast cell line were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: MTT Assay, Flow Cytometry, Transwell Assay, Binding Assay, Small Interfering RNA

Journal: Oncology Letters

Article Title: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

doi: 10.3892/ol.2016.5400

Figure Lengend Snippet: Effects of Oct4 downregulation on the expression levels of numerous lncRNAs. F5M2 cells were treated with Oct4 siRNA1 or scramble for 3 days. The expressions of lncRNAs were measured by real-time RT-PCR (n=6). *P<0.01 vs. scramble. lncRNA, long non-coding RNA; siRNA, small interfering RNA; Oct4, octamer-binding transcription factor 4; BCAR4, breast cancer anti-estrogen resistance 4; CASC2, cancer susceptibility candidate 2; HIF1A-AS1, HIF1A antisense RNA 1; PCA3, prostate cancer associated 3; PVT1, Pvt1 oncogene; SNHG5, small nucleolar RNA host gene 5.

Article Snippet: SOSP-9607, MG63 and F5M2 osteosarcoma cell lines and the hFOB1.19 osteoblast cell line were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Small Interfering RNA, Binding Assay

Journal: Oncology Letters

Article Title: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

doi: 10.3892/ol.2016.5400

Figure Lengend Snippet: AK055347 downregulation decreases proliferation and invasion ability of F5M2 cells. F5M2 cells were treated with of AK055347 siRNA3 or scramble for 3 days. (A) Proliferation was measured by MTT assay, (B) cell apoptosis was assayed with Annexin V by flow cytometry and (C) invasion ability was evaluated by Transwell assay (n=6). *P<0.05, **P<0.01 vs. the scramble group. (D) AK055347 downregulation decreased cell invasion ability. Magnification, ×200. siRNA, small interfering RNA; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: SOSP-9607, MG63 and F5M2 osteosarcoma cell lines and the hFOB1.19 osteoblast cell line were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: MTT Assay, Flow Cytometry, Transwell Assay, Small Interfering RNA