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Bioneer Corporation dntps
Dntps, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dntps - by Bioz Stars, 2020-03
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Related Articles

Diagnostic Assay:

Article Title: Genetic diversity among Trypanosoma vivax strains detected in naturally infected cattle in Nigeria based on ITS1 of rDNA and diagnostic antigen gene sequences
Article Snippet: T. vivax species specific primer set (ILO1264: 5′-CAGCTCGCCGAAGGCCACTTGGCTGGG-3′ and ILO1265: 5′-TCGCTACCACAGTCGCAATCGTCGTCTCAAGG-3′) for a 400 bp diagnostic antigen gene fragment (Masake et al. ) and generic primers set (ITS CF: 5′-CCGGAAGTTCACCGATATTG-3′ and ITS BR: 5′-TTGCTGCGTTCTTCAACGAA-3′) for 250 bp internal transcribed spacer 1 fragment (Njiru et al. ) were used. .. The PCR amplifications were carried out in 20 µl final volume containing equivalent of 20 ng of genomic DNA, 10 mM Tris–HCl, pH 8.3, 1.5 mM MgCl2 , 50 µM KCl, 200 µM each of dNTPs, 40 ng of each of the primers and 1 unit of Taq DNA polymerase (Bioneer, Inc. Alameda, CA USA).

Clone Assay:

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: Paragraph title: Cloning putative surfactin biosynthesis genes from the genomic library ... Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

Amplification:

Article Title: New Record of the Genus Calyptella from Korea
Article Snippet: ITS1 and ITS4 primer sets were used for amplification of internal transcribed spacer (ITS) regions ( ). .. Contaminating primers and dNTPs were removed from PCR products using the High Pure PCR Product Purification Kit (Bioneer Co., Chungbuk, Korea).

Article Title: Diversity in Accessions of Panicum miliaceum L. Based on Agro-Morphological, Antioxidative, and Genetic Traits
Article Snippet: .. RAPD amplification reactions were performed in a 20-µL reaction volume containing 50 ng genomic DNA, 2 µL 1X PCR buffer, 1 U Taq DNA polymerase, 1 µM of primer, and 300 µM of dNTPs obtained from Bioneer (Daejeon, South Korea). ..

Article Title: Overexpression of peroxiredoxin-3 and −5 is a potential biomarker for prognosis in endometrial cancer
Article Snippet: Each reaction contained 10 pM of each primer, 1.5 mM MgCl2 , 250 µM dNTPs, 10 mM Tris-HCl (pH 9.0), 30 mM KCl, and 1 unit Top DNA polymerase (AccuPower® PCR PreMix; cat. no. K-2012; Bioneer Corporation, Daejeon, Korea). .. Amplification was conducted with the following thermal cycling conditions: 5 min at 95°C; 35 cycles of amplification consisting of 30 sec at 95°C, 40 sec at 55°C, and 30 sec at 72°C; and a final extension at 72°C for 5 min. For analysis, 5 µl of the product was subjected to agarose gel electrophoresis and visualized by ethidium bromide.

Article Title: Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
Article Snippet: A RAPD marker was converted into a sequence characterized amplified region (SCAR) marker using sequence information. .. The multiplex PCR was performed with a DNA Thermal Cycler (Takara Shozo, Japan) under the following conditions: an initial denaturation at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 7 min. Each reaction mixture contained a 1x PCR buffer (10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCl2 , pH 9.0), 0.2 mM dNTPs, 10 pmol of each primers, 1.25 U of Top DNA polymerase (Bioneer, Korea), and 10 ng of template DNA in a volume of 50 μl.

Article Title: Diversity in Accessions of Panicum miliaceum L. Based on Agro-Morphological, Antioxidative, and Genetic Traits
Article Snippet: .. RAPD amplification reactions were performed in a 20-µL reaction volume containing 50 ng genomic DNA, 2 µL 1X PCR buffer, 1 U Taq DNA polymerase, 1 µM of primer, and 300 µM of dNTPs obtained from Bioneer (Daejeon, South Korea). ..

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: To clone the putative surfactin biosynthesis genes from the genomic DNA of B . subtilis 168 and B . subtilis subsp. krictiensis , we amplified the genes by PCR using primers B9 ( 5′- GCAAAATTTCCGGACAGCGGGATAT-3ʹ ) and B10 ( 5′-TCGATCCGGCCGATGTATTCGAT-3ʹ ). .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

Article Title: SLC11A1 polymorphisms and host susceptibility to cutaneous leishmaniasis in Pakistan
Article Snippet: The reaction contained 200 ng of genomic DNA, 0.4 μM of each forward outer primer (5′-CTG CGG AAG CTG GAA GCT TTT TTT GGA C-3′) and T-allele specific reverse inner primer (5′-GAG GAA TGA TCT TGG GAG GTC CAC AGT TGA-3′), 0.2 μM of reverse outer primer (5′-CAC ATA CTG CAG GGA GGA GCC TGG TCA G-3′), 200 μM dNTPs each, 1.2 mM MgCl2 , 1× PCR buffer (400 mM KCl and 100 mM Tris-HCl, pH 9.0) and 1 unit of Taq DNA polymerase (Bioneer). .. Amplification was performed using Multi Block System 0.2S (ThermoHybaid) with the following conditions: initial denaturation at 94 °C for 4 min, followed by 35 cycles comprising denaturation at 94 °C for 30 s, annealing at 56 °C for 35 s and extension at 72 °C for 30 s. The last cycle was followed by a final extension at 72 °C for 7 min.

Article Title: Occurrence of novel GII.17 and GII.21 norovirus variants in the coastal environment of South Korea in 2015
Article Snippet: Paragraph title: Nucleic acid amplification ... The first PCR products (5 μL) were mixed with 10× buffer, 4 μL of 2.5 mM dNTPs, 50 μM forward/reverse primers (GIIF3M/GIIRIM for region C, GIIPF750M/GIIRIM for the ORF1-ORF2 junction, or GII.F3M/GIICR1450 for the nearly complete VP1; ) [ ], and 5 U of Top DNA polymerase (Bioneer, Daejeon, South Korea).

Synthesized:

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: The primers were synthesized and purified by Bioneer and the PCR was performed on an i-Cycler (Bio-Rad). .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

Article Title: Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Article Snippet: .. The reaction mixture contained 5 µL of 5× RT-PCR buffer (including 2.5 mM MgCl2 ), 0.4 mM dNTPs, 0.5 µM of each of the four primers (synthesized in Bioneer, Korea), 1 µL of the enzyme mix, and 5 µL of RNA in a final volume of 25 µL. .. The RT-PCR conditions were as follows: a reverse transcription step at 50℃ for 30 min, reverse transcriptase inactivation and initial PCR activation at 95℃ for 15 min; 35 cycles of denaturation at 94℃ for 20 seconds, annealing at 55℃ for 20 seconds, and extension at 72℃ for 30 seconds; and a final elongation step at 72℃ for 10 min. T3000 Thermocycler (Biometra, Germany) was used for the RT-PCR.

Quantitative RT-PCR:

Article Title: Kazinol U inhibits melanogenesis through the inhibition of tyrosinase‐related proteins via AMP kinase activation
Article Snippet: Paragraph title: RNA isolation and real‐time RT‐PCR ... For the RT reaction, first‐strand cDNA was generated using M‐MLV reverse transcriptase (Promega, Madison, WI), oligo‐(dT) primers and dNTPs (Bioneer, Daejeon, Republic of Korea).

SYBR Green Assay:

Article Title: Kazinol U inhibits melanogenesis through the inhibition of tyrosinase‐related proteins via AMP kinase activation
Article Snippet: For the RT reaction, first‐strand cDNA was generated using M‐MLV reverse transcriptase (Promega, Madison, WI), oligo‐(dT) primers and dNTPs (Bioneer, Daejeon, Republic of Korea). .. Quantitative real‐time PCR was performed using an ABI StepOnePlus™ real‐time PCR thermal cycler with Power SYBR Green PCR Master Mix according to the manufacturer's instructions (Applied Biosystems, CA).

Incubation:

Article Title: NDRG2-mediated Modulation of SOCS3 and STAT3 Activity Inhibits IL-10 Production
Article Snippet: For the RT reaction, RNA was first incubated with an oligo-(dT) primer at 70℃ to denature the RNA secondary structure and then incubated at room temperature for 10 min to allow the primer to anneal to the RNA. .. Other components of the RT assay, including dNTPs (Bioneer, Daejeon, Korea), M-MLB reverse transcriptase, and RT buffer (Promega, Madison, WI), were then added to the reaction.

Concentration Assay:

Article Title: Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species
Article Snippet: .. LAMP was carried out in a total reaction volume of 25 μl with a final concentration of 2 μM for both FIP and BIP and 0.2 μM for both F3 and B3, 0.8 mM concentrations of dNTPs (Bioneer), 2.5 μl of 1× ThermoPol reaction buffer [20 mM Tris-HCl, 10 mM (NH4 )2 SO4 , 10 mM KCl, 2 mM MgSO4 , 0.1% Triton X-100] (New England BioLabs Inc., Ipswich, United Kingdom), 8 U of Bst DNA polymerase large fragment (New England BioLabs Inc.), 2 μl of DNA template, and diethylpyrocarbonate (DEPC)-treated deionized water to bring the reaction volume to 25 μl. ..

Reverse Transcription Polymerase Chain Reaction:

Article Title: Occurrence of novel GII.17 and GII.21 norovirus variants in the coastal environment of South Korea in 2015
Article Snippet: A final extension was performed at 72°C for 7 min. For virus isolation process control in clams (control murine NoV RNA from digestive gland tissue), primers and RT-PCR conditions followed a previous description [ ]. .. The first PCR products (5 μL) were mixed with 10× buffer, 4 μL of 2.5 mM dNTPs, 50 μM forward/reverse primers (GIIF3M/GIIRIM for region C, GIIPF750M/GIIRIM for the ORF1-ORF2 junction, or GII.F3M/GIICR1450 for the nearly complete VP1; ) [ ], and 5 U of Top DNA polymerase (Bioneer, Daejeon, South Korea).

Article Title: Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Article Snippet: .. The reaction mixture contained 5 µL of 5× RT-PCR buffer (including 2.5 mM MgCl2 ), 0.4 mM dNTPs, 0.5 µM of each of the four primers (synthesized in Bioneer, Korea), 1 µL of the enzyme mix, and 5 µL of RNA in a final volume of 25 µL. .. The RT-PCR conditions were as follows: a reverse transcription step at 50℃ for 30 min, reverse transcriptase inactivation and initial PCR activation at 95℃ for 15 min; 35 cycles of denaturation at 94℃ for 20 seconds, annealing at 55℃ for 20 seconds, and extension at 72℃ for 30 seconds; and a final elongation step at 72℃ for 10 min. T3000 Thermocycler (Biometra, Germany) was used for the RT-PCR.

Article Title: Antioxidant and Anti-inflammatory Activities of Broccoli Florets in LPS-stimulated RAW 264.7 Cells
Article Snippet: Paragraph title: RT-PCR analysis ... The reactions were conducted in 25-μL volumes containing Taq DNA polymerase, dNTPs, reaction buffer, and primers ( ) (Bioneer, Daejeon, Korea).

Generated:

Article Title: Kazinol U inhibits melanogenesis through the inhibition of tyrosinase‐related proteins via AMP kinase activation
Article Snippet: .. For the RT reaction, first‐strand cDNA was generated using M‐MLV reverse transcriptase (Promega, Madison, WI), oligo‐(dT) primers and dNTPs (Bioneer, Daejeon, Republic of Korea). ..

DNA Sequencing:

Article Title: New Record of the Genus Calyptella from Korea
Article Snippet: Paragraph title: Genomic DNA isolation, PCR and DNA sequencing ... Contaminating primers and dNTPs were removed from PCR products using the High Pure PCR Product Purification Kit (Bioneer Co., Chungbuk, Korea).

Sequencing:

Article Title: New Record of the Genus Calyptella from Korea
Article Snippet: Contaminating primers and dNTPs were removed from PCR products using the High Pure PCR Product Purification Kit (Bioneer Co., Chungbuk, Korea). .. Big Dye Terminator Kit and ABI Prism 310 Genetic Analyzer (Perkin Elmer, New Jersey, USA) was used for sequencing.

Article Title: Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
Article Snippet: A RAPD marker was converted into a sequence characterized amplified region (SCAR) marker using sequence information. .. The multiplex PCR was performed with a DNA Thermal Cycler (Takara Shozo, Japan) under the following conditions: an initial denaturation at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 7 min. Each reaction mixture contained a 1x PCR buffer (10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCl2 , pH 9.0), 0.2 mM dNTPs, 10 pmol of each primers, 1.25 U of Top DNA polymerase (Bioneer, Korea), and 10 ng of template DNA in a volume of 50 μl.

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: Cloning putative surfactin biosynthesis genes from the genomic library To obtain the surfactin biosynthesis genes from the wild-type B . subtilis genomic library, two PCR primers were designed using the sequence of the surfactin biosynthesis genes of B . subtilis 168, which was used for the Bacillus genome project and contains a surfactin synthetase gene. .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

DNA Extraction:

Article Title: New Record of the Genus Calyptella from Korea
Article Snippet: Paragraph title: Genomic DNA isolation, PCR and DNA sequencing ... Contaminating primers and dNTPs were removed from PCR products using the High Pure PCR Product Purification Kit (Bioneer Co., Chungbuk, Korea).

Article Title: Genetic diversity among Trypanosoma vivax strains detected in naturally infected cattle in Nigeria based on ITS1 of rDNA and diagnostic antigen gene sequences
Article Snippet: Paragraph title: DNA extraction and Trypanosoma vivax detection ... The PCR amplifications were carried out in 20 µl final volume containing equivalent of 20 ng of genomic DNA, 10 mM Tris–HCl, pH 8.3, 1.5 mM MgCl2 , 50 µM KCl, 200 µM each of dNTPs, 40 ng of each of the primers and 1 unit of Taq DNA polymerase (Bioneer, Inc. Alameda, CA USA).

Marker:

Article Title: Identification of Cucumber mosaic resistance 2 (cmr2) That Confers Resistance to a New Cucumber mosaic virus Isolate P1 (CMV-P1) in Pepper (Capsicum spp.)
Article Snippet: Paragraph title: High-Resolution Melting Marker Polymorphism Survey ... PCR was carried out in 20-μL reaction volumes with 50 ng genomic DNA as template, 1× HiPi buffer (ELPIS-Biotech, South Korea), 0.2 mM dNTPs, 500 mM each forward and reverse primer (Bioneer, South Korea), 1.5 μM SYTO9 (Invitrogen, United States), and 0.6 units home-made Taq DNA polymerase.

Article Title: Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
Article Snippet: A RAPD marker was converted into a sequence characterized amplified region (SCAR) marker using sequence information. .. The multiplex PCR was performed with a DNA Thermal Cycler (Takara Shozo, Japan) under the following conditions: an initial denaturation at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 7 min. Each reaction mixture contained a 1x PCR buffer (10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCl2 , pH 9.0), 0.2 mM dNTPs, 10 pmol of each primers, 1.25 U of Top DNA polymerase (Bioneer, Korea), and 10 ng of template DNA in a volume of 50 μl.

Isolation:

Article Title: New Record of the Genus Calyptella from Korea
Article Snippet: Genomic DNA isolation, PCR and DNA sequencing DNA was isolated from pure cultures of fungus using a SDS-CTAB (sodium dodecyl sulfate-cetyltrimethyl-ammonium bromide) method ( ). .. Contaminating primers and dNTPs were removed from PCR products using the High Pure PCR Product Purification Kit (Bioneer Co., Chungbuk, Korea).

Article Title: Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
Article Snippet: The multiplex PCR was performed with a DNA Thermal Cycler (Takara Shozo, Japan) under the following conditions: an initial denaturation at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 7 min. Each reaction mixture contained a 1x PCR buffer (10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCl2 , pH 9.0), 0.2 mM dNTPs, 10 pmol of each primers, 1.25 U of Top DNA polymerase (Bioneer, Korea), and 10 ng of template DNA in a volume of 50 μl. .. The four new haplotype strains, including two strains isolated in Korea (SYS1, SYS4) and two obtained from Italy (IHL1, IKB4), produced an expected 545-bp amplicon (lanes 1 to 4), while the two Korean (CJW3, JYG6) and two Japanese strains (Kw11, PaB1) did not amplify this DNA segment with primer pair Tac F/R (lanes 5 to 8).

Article Title: NDRG2-mediated Modulation of SOCS3 and STAT3 Activity Inhibits IL-10 Production
Article Snippet: Reverse transcription (RT) Total RNA from harvested cells was isolated using TRI reagent (Molecular Research Center, Cincinnati, OH) and 5µg of total RNA was reverse transcribed (RT) into cDNA. .. Other components of the RT assay, including dNTPs (Bioneer, Daejeon, Korea), M-MLB reverse transcriptase, and RT buffer (Promega, Madison, WI), were then added to the reaction.

Article Title: Kazinol U inhibits melanogenesis through the inhibition of tyrosinase‐related proteins via AMP kinase activation
Article Snippet: Paragraph title: RNA isolation and real‐time RT‐PCR ... For the RT reaction, first‐strand cDNA was generated using M‐MLV reverse transcriptase (Promega, Madison, WI), oligo‐(dT) primers and dNTPs (Bioneer, Daejeon, Republic of Korea).

Size-exclusion Chromatography:

Article Title: Overexpression of peroxiredoxin-3 and −5 is a potential biomarker for prognosis in endometrial cancer
Article Snippet: Each reaction contained 10 pM of each primer, 1.5 mM MgCl2 , 250 µM dNTPs, 10 mM Tris-HCl (pH 9.0), 30 mM KCl, and 1 unit Top DNA polymerase (AccuPower® PCR PreMix; cat. no. K-2012; Bioneer Corporation, Daejeon, Korea). .. Amplification was conducted with the following thermal cycling conditions: 5 min at 95°C; 35 cycles of amplification consisting of 30 sec at 95°C, 40 sec at 55°C, and 30 sec at 72°C; and a final extension at 72°C for 5 min. For analysis, 5 µl of the product was subjected to agarose gel electrophoresis and visualized by ethidium bromide.

Purification:

Article Title: New Record of the Genus Calyptella from Korea
Article Snippet: .. Contaminating primers and dNTPs were removed from PCR products using the High Pure PCR Product Purification Kit (Bioneer Co., Chungbuk, Korea). .. Big Dye Terminator Kit and ABI Prism 310 Genetic Analyzer (Perkin Elmer, New Jersey, USA) was used for sequencing.

Article Title: Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
Article Snippet: The purified DNA was ligated into a pGEM-T Easy vector (Promega, USA) following the manufacturer’s instructions. .. The multiplex PCR was performed with a DNA Thermal Cycler (Takara Shozo, Japan) under the following conditions: an initial denaturation at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 7 min. Each reaction mixture contained a 1x PCR buffer (10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCl2 , pH 9.0), 0.2 mM dNTPs, 10 pmol of each primers, 1.25 U of Top DNA polymerase (Bioneer, Korea), and 10 ng of template DNA in a volume of 50 μl.

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: The primers were synthesized and purified by Bioneer and the PCR was performed on an i-Cycler (Bio-Rad). .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

Polymerase Chain Reaction:

Article Title: New Record of the Genus Calyptella from Korea
Article Snippet: .. Contaminating primers and dNTPs were removed from PCR products using the High Pure PCR Product Purification Kit (Bioneer Co., Chungbuk, Korea). .. Big Dye Terminator Kit and ABI Prism 310 Genetic Analyzer (Perkin Elmer, New Jersey, USA) was used for sequencing.

Article Title: Diversity in Accessions of Panicum miliaceum L. Based on Agro-Morphological, Antioxidative, and Genetic Traits
Article Snippet: .. RAPD amplification reactions were performed in a 20-µL reaction volume containing 50 ng genomic DNA, 2 µL 1X PCR buffer, 1 U Taq DNA polymerase, 1 µM of primer, and 300 µM of dNTPs obtained from Bioneer (Daejeon, South Korea). ..

Article Title: Genetic diversity among Trypanosoma vivax strains detected in naturally infected cattle in Nigeria based on ITS1 of rDNA and diagnostic antigen gene sequences
Article Snippet: .. The PCR amplifications were carried out in 20 µl final volume containing equivalent of 20 ng of genomic DNA, 10 mM Tris–HCl, pH 8.3, 1.5 mM MgCl2 , 50 µM KCl, 200 µM each of dNTPs, 40 ng of each of the primers and 1 unit of Taq DNA polymerase (Bioneer, Inc. Alameda, CA USA). .. The reactions were placed in a C-1000 series thermocycler (Biorad, Hercules, CA, USA).

Article Title: Overexpression of peroxiredoxin-3 and −5 is a potential biomarker for prognosis in endometrial cancer
Article Snippet: .. Each reaction contained 10 pM of each primer, 1.5 mM MgCl2 , 250 µM dNTPs, 10 mM Tris-HCl (pH 9.0), 30 mM KCl, and 1 unit Top DNA polymerase (AccuPower® PCR PreMix; cat. no. K-2012; Bioneer Corporation, Daejeon, Korea). .. Reactions were performed on a T100 Thermal Cycler (Bio-Rad, Hercules, CA, USA).

Article Title: Identification of Cucumber mosaic resistance 2 (cmr2) That Confers Resistance to a New Cucumber mosaic virus Isolate P1 (CMV-P1) in Pepper (Capsicum spp.)
Article Snippet: .. PCR was carried out in 20-μL reaction volumes with 50 ng genomic DNA as template, 1× HiPi buffer (ELPIS-Biotech, South Korea), 0.2 mM dNTPs, 500 mM each forward and reverse primer (Bioneer, South Korea), 1.5 μM SYTO9 (Invitrogen, United States), and 0.6 units home-made Taq DNA polymerase. .. PCR conditions were: initial denaturation at 94°C for 2 min followed by 35 cycles of 94°C for 30 s, 55°C for 1 min, and 72°C for 1 min. PCR for HRM analysis was carried out in 20 μL reaction volumes containing 20 ng of genomic DNA, 6 pmol of each primer, and 2× HRM master mix (Roche, Basel, Switzerland).

Article Title: Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
Article Snippet: .. The multiplex PCR was performed with a DNA Thermal Cycler (Takara Shozo, Japan) under the following conditions: an initial denaturation at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 7 min. Each reaction mixture contained a 1x PCR buffer (10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCl2 , pH 9.0), 0.2 mM dNTPs, 10 pmol of each primers, 1.25 U of Top DNA polymerase (Bioneer, Korea), and 10 ng of template DNA in a volume of 50 μl. .. Results employing two primer pairs in a multiplex PCR with eight P. syringae pv. actinidiae and three P. syringae pv. theae are shown in .

Article Title: NDRG2-mediated Modulation of SOCS3 and STAT3 Activity Inhibits IL-10 Production
Article Snippet: Other components of the RT assay, including dNTPs (Bioneer, Daejeon, Korea), M-MLB reverse transcriptase, and RT buffer (Promega, Madison, WI), were then added to the reaction. .. The RT product was then used as a template for a 20µl PCR reaction.

Article Title: Diversity in Accessions of Panicum miliaceum L. Based on Agro-Morphological, Antioxidative, and Genetic Traits
Article Snippet: .. RAPD amplification reactions were performed in a 20-µL reaction volume containing 50 ng genomic DNA, 2 µL 1X PCR buffer, 1 U Taq DNA polymerase, 1 µM of primer, and 300 µM of dNTPs obtained from Bioneer (Daejeon, South Korea). ..

Article Title: Kazinol U inhibits melanogenesis through the inhibition of tyrosinase‐related proteins via AMP kinase activation
Article Snippet: For the RT reaction, first‐strand cDNA was generated using M‐MLV reverse transcriptase (Promega, Madison, WI), oligo‐(dT) primers and dNTPs (Bioneer, Daejeon, Republic of Korea). .. Quantitative real‐time PCR was performed using an ABI StepOnePlus™ real‐time PCR thermal cycler with Power SYBR Green PCR Master Mix according to the manufacturer's instructions (Applied Biosystems, CA).

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer). ..

Article Title: SLC11A1 polymorphisms and host susceptibility to cutaneous leishmaniasis in Pakistan
Article Snippet: .. The reaction contained 200 ng of genomic DNA, 0.4 μM of each forward outer primer (5′-CTG CGG AAG CTG GAA GCT TTT TTT GGA C-3′) and T-allele specific reverse inner primer (5′-GAG GAA TGA TCT TGG GAG GTC CAC AGT TGA-3′), 0.2 μM of reverse outer primer (5′-CAC ATA CTG CAG GGA GGA GCC TGG TCA G-3′), 200 μM dNTPs each, 1.2 mM MgCl2 , 1× PCR buffer (400 mM KCl and 100 mM Tris-HCl, pH 9.0) and 1 unit of Taq DNA polymerase (Bioneer). .. Amplification was performed using Multi Block System 0.2S (ThermoHybaid) with the following conditions: initial denaturation at 94 °C for 4 min, followed by 35 cycles comprising denaturation at 94 °C for 30 s, annealing at 56 °C for 35 s and extension at 72 °C for 30 s. The last cycle was followed by a final extension at 72 °C for 7 min.

Article Title: Occurrence of novel GII.17 and GII.21 norovirus variants in the coastal environment of South Korea in 2015
Article Snippet: .. The first PCR products (5 μL) were mixed with 10× buffer, 4 μL of 2.5 mM dNTPs, 50 μM forward/reverse primers (GIIF3M/GIIRIM for region C, GIIPF750M/GIIRIM for the ORF1-ORF2 junction, or GII.F3M/GIICR1450 for the nearly complete VP1; ) [ ], and 5 U of Top DNA polymerase (Bioneer, Daejeon, South Korea). ..

Article Title: Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Article Snippet: The reaction mixture contained 5 µL of 5× RT-PCR buffer (including 2.5 mM MgCl2 ), 0.4 mM dNTPs, 0.5 µM of each of the four primers (synthesized in Bioneer, Korea), 1 µL of the enzyme mix, and 5 µL of RNA in a final volume of 25 µL. .. The RT-PCR conditions were as follows: a reverse transcription step at 50℃ for 30 min, reverse transcriptase inactivation and initial PCR activation at 95℃ for 15 min; 35 cycles of denaturation at 94℃ for 20 seconds, annealing at 55℃ for 20 seconds, and extension at 72℃ for 30 seconds; and a final elongation step at 72℃ for 10 min. T3000 Thermocycler (Biometra, Germany) was used for the RT-PCR.

Article Title: Antioxidant and Anti-inflammatory Activities of Broccoli Florets in LPS-stimulated RAW 264.7 Cells
Article Snippet: PCR was conducted on aliquots of the resulting cDNA preparation to detect iNOS, TNF-α, IL-1β, IL-6, and β-actin mRNA. .. The reactions were conducted in 25-μL volumes containing Taq DNA polymerase, dNTPs, reaction buffer, and primers ( ) (Bioneer, Daejeon, Korea).

Blocking Assay:

Article Title: SLC11A1 polymorphisms and host susceptibility to cutaneous leishmaniasis in Pakistan
Article Snippet: The reaction contained 200 ng of genomic DNA, 0.4 μM of each forward outer primer (5′-CTG CGG AAG CTG GAA GCT TTT TTT GGA C-3′) and T-allele specific reverse inner primer (5′-GAG GAA TGA TCT TGG GAG GTC CAC AGT TGA-3′), 0.2 μM of reverse outer primer (5′-CAC ATA CTG CAG GGA GGA GCC TGG TCA G-3′), 200 μM dNTPs each, 1.2 mM MgCl2 , 1× PCR buffer (400 mM KCl and 100 mM Tris-HCl, pH 9.0) and 1 unit of Taq DNA polymerase (Bioneer). .. Amplification was performed using Multi Block System 0.2S (ThermoHybaid) with the following conditions: initial denaturation at 94 °C for 4 min, followed by 35 cycles comprising denaturation at 94 °C for 30 s, annealing at 56 °C for 35 s and extension at 72 °C for 30 s. The last cycle was followed by a final extension at 72 °C for 7 min.

Chloramphenicol Acetyltransferase Assay:

Article Title: Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species
Article Snippet: LAMP primers specific for the A. butzleri 23S gene (GenBank no. ) were designed by the online LAMP primer design software PrimerExplorer V4 ( ) and were as follows: F3, 5′-ACT GTG ACA ACC AGG AGG TT-3′; B3, 5′-TCC AAC GCT CCT TAC CGG-3′; FIP, 5′-CGC GCA GAA TCA CTA GAC CAG TGG CTT AGA AGC AGC CAT CC-3′; and BIP, 5′-AAC GGG GCT AAG ATG TAC ACC GAC GCT GAA TAG AAC GCT CTC-3′. .. LAMP was carried out in a total reaction volume of 25 μl with a final concentration of 2 μM for both FIP and BIP and 0.2 μM for both F3 and B3, 0.8 mM concentrations of dNTPs (Bioneer), 2.5 μl of 1× ThermoPol reaction buffer [20 mM Tris-HCl, 10 mM (NH4 )2 SO4 , 10 mM KCl, 2 mM MgSO4 , 0.1% Triton X-100] (New England BioLabs Inc., Ipswich, United Kingdom), 8 U of Bst DNA polymerase large fragment (New England BioLabs Inc.), 2 μl of DNA template, and diethylpyrocarbonate (DEPC)-treated deionized water to bring the reaction volume to 25 μl.

Plasmid Preparation:

Article Title: Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
Article Snippet: The purified DNA was ligated into a pGEM-T Easy vector (Promega, USA) following the manufacturer’s instructions. .. The multiplex PCR was performed with a DNA Thermal Cycler (Takara Shozo, Japan) under the following conditions: an initial denaturation at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 7 min. Each reaction mixture contained a 1x PCR buffer (10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCl2 , pH 9.0), 0.2 mM dNTPs, 10 pmol of each primers, 1.25 U of Top DNA polymerase (Bioneer, Korea), and 10 ng of template DNA in a volume of 50 μl.

Software:

Article Title: Overexpression of peroxiredoxin-3 and −5 is a potential biomarker for prognosis in endometrial cancer
Article Snippet: Each reaction contained 10 pM of each primer, 1.5 mM MgCl2 , 250 µM dNTPs, 10 mM Tris-HCl (pH 9.0), 30 mM KCl, and 1 unit Top DNA polymerase (AccuPower® PCR PreMix; cat. no. K-2012; Bioneer Corporation, Daejeon, Korea). .. PCR bands were quantified using the Multi Gauge V2.2 software program (FUJI PHOTO FILM, Japan).

Article Title: Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species
Article Snippet: LAMP primers specific for the A. butzleri 23S gene (GenBank no. ) were designed by the online LAMP primer design software PrimerExplorer V4 ( ) and were as follows: F3, 5′-ACT GTG ACA ACC AGG AGG TT-3′; B3, 5′-TCC AAC GCT CCT TAC CGG-3′; FIP, 5′-CGC GCA GAA TCA CTA GAC CAG TGG CTT AGA AGC AGC CAT CC-3′; and BIP, 5′-AAC GGG GCT AAG ATG TAC ACC GAC GCT GAA TAG AAC GCT CTC-3′. .. LAMP was carried out in a total reaction volume of 25 μl with a final concentration of 2 μM for both FIP and BIP and 0.2 μM for both F3 and B3, 0.8 mM concentrations of dNTPs (Bioneer), 2.5 μl of 1× ThermoPol reaction buffer [20 mM Tris-HCl, 10 mM (NH4 )2 SO4 , 10 mM KCl, 2 mM MgSO4 , 0.1% Triton X-100] (New England BioLabs Inc., Ipswich, United Kingdom), 8 U of Bst DNA polymerase large fragment (New England BioLabs Inc.), 2 μl of DNA template, and diethylpyrocarbonate (DEPC)-treated deionized water to bring the reaction volume to 25 μl.

Real-time Polymerase Chain Reaction:

Article Title: Kazinol U inhibits melanogenesis through the inhibition of tyrosinase‐related proteins via AMP kinase activation
Article Snippet: For the RT reaction, first‐strand cDNA was generated using M‐MLV reverse transcriptase (Promega, Madison, WI), oligo‐(dT) primers and dNTPs (Bioneer, Daejeon, Republic of Korea). .. Quantitative real‐time PCR was performed using an ABI StepOnePlus™ real‐time PCR thermal cycler with Power SYBR Green PCR Master Mix according to the manufacturer's instructions (Applied Biosystems, CA).

Multiplex Assay:

Article Title: Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
Article Snippet: .. The multiplex PCR was performed with a DNA Thermal Cycler (Takara Shozo, Japan) under the following conditions: an initial denaturation at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 7 min. Each reaction mixture contained a 1x PCR buffer (10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCl2 , pH 9.0), 0.2 mM dNTPs, 10 pmol of each primers, 1.25 U of Top DNA polymerase (Bioneer, Korea), and 10 ng of template DNA in a volume of 50 μl. .. Results employing two primer pairs in a multiplex PCR with eight P. syringae pv. actinidiae and three P. syringae pv. theae are shown in .

Agarose Gel Electrophoresis:

Article Title: Genetic diversity among Trypanosoma vivax strains detected in naturally infected cattle in Nigeria based on ITS1 of rDNA and diagnostic antigen gene sequences
Article Snippet: The PCR amplifications were carried out in 20 µl final volume containing equivalent of 20 ng of genomic DNA, 10 mM Tris–HCl, pH 8.3, 1.5 mM MgCl2 , 50 µM KCl, 200 µM each of dNTPs, 40 ng of each of the primers and 1 unit of Taq DNA polymerase (Bioneer, Inc. Alameda, CA USA). .. Ten microliters of the PCR products were electrophoresed through 1 % agarose gel in 1× TBE (89 mM Tris, 89 mM boric acid 1 mM EDTA) at 90 V for 80 min along with 10 µl of GENE Mate Quanti-Marker 100 bp DNA ladder (BioExpress, Kaysville, UT, USA).

Article Title: Overexpression of peroxiredoxin-3 and −5 is a potential biomarker for prognosis in endometrial cancer
Article Snippet: Each reaction contained 10 pM of each primer, 1.5 mM MgCl2 , 250 µM dNTPs, 10 mM Tris-HCl (pH 9.0), 30 mM KCl, and 1 unit Top DNA polymerase (AccuPower® PCR PreMix; cat. no. K-2012; Bioneer Corporation, Daejeon, Korea). .. Amplification was conducted with the following thermal cycling conditions: 5 min at 95°C; 35 cycles of amplification consisting of 30 sec at 95°C, 40 sec at 55°C, and 30 sec at 72°C; and a final extension at 72°C for 5 min. For analysis, 5 µl of the product was subjected to agarose gel electrophoresis and visualized by ethidium bromide.

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer). .. Then, an aliquot (20 μL) of the amplification products was separated on a 1% agarose gel (SeaKem LE agarose, Lonza) in 1× TAE buffer (40 mM Tris-acetate, 1 mM EDTA, pH 8.0).

Electrophoresis:

Article Title: Diversity in Accessions of Panicum miliaceum L. Based on Agro-Morphological, Antioxidative, and Genetic Traits
Article Snippet: Paragraph title: PCR Amplification and Electrophoresis ... RAPD amplification reactions were performed in a 20-µL reaction volume containing 50 ng genomic DNA, 2 µL 1X PCR buffer, 1 U Taq DNA polymerase, 1 µM of primer, and 300 µM of dNTPs obtained from Bioneer (Daejeon, South Korea).

Article Title: Diversity in Accessions of Panicum miliaceum L. Based on Agro-Morphological, Antioxidative, and Genetic Traits
Article Snippet: Paragraph title: PCR Amplification and Electrophoresis ... RAPD amplification reactions were performed in a 20-µL reaction volume containing 50 ng genomic DNA, 2 µL 1X PCR buffer, 1 U Taq DNA polymerase, 1 µM of primer, and 300 µM of dNTPs obtained from Bioneer (Daejeon, South Korea).

Article Title: Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Article Snippet: The reaction mixture contained 5 µL of 5× RT-PCR buffer (including 2.5 mM MgCl2 ), 0.4 mM dNTPs, 0.5 µM of each of the four primers (synthesized in Bioneer, Korea), 1 µL of the enzyme mix, and 5 µL of RNA in a final volume of 25 µL. .. The amplicons were separated using electrophoresis in 1.5% agarose gels and stained with ethidium bromide.

Spectrophotometry:

Article Title: Genetic diversity among Trypanosoma vivax strains detected in naturally infected cattle in Nigeria based on ITS1 of rDNA and diagnostic antigen gene sequences
Article Snippet: Quantification of DNA yield and assessment of quality were done using Nanodrop ND-1000 UV/Vis spectrophotometer (Nanodrop Technologies, Inc., DE, USA). .. The PCR amplifications were carried out in 20 µl final volume containing equivalent of 20 ng of genomic DNA, 10 mM Tris–HCl, pH 8.3, 1.5 mM MgCl2 , 50 µM KCl, 200 µM each of dNTPs, 40 ng of each of the primers and 1 unit of Taq DNA polymerase (Bioneer, Inc. Alameda, CA USA).

Produced:

Article Title: Molecular Characteristics of Pseudomonas syringae pv. actinidiae Strains Isolated in Korea and a Multiplex PCR Assay for Haplotype Differentiation
Article Snippet: The multiplex PCR was performed with a DNA Thermal Cycler (Takara Shozo, Japan) under the following conditions: an initial denaturation at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 7 min. Each reaction mixture contained a 1x PCR buffer (10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCl2 , pH 9.0), 0.2 mM dNTPs, 10 pmol of each primers, 1.25 U of Top DNA polymerase (Bioneer, Korea), and 10 ng of template DNA in a volume of 50 μl. .. The four new haplotype strains, including two strains isolated in Korea (SYS1, SYS4) and two obtained from Italy (IHL1, IKB4), produced an expected 545-bp amplicon (lanes 1 to 4), while the two Korean (CJW3, JYG6) and two Japanese strains (Kw11, PaB1) did not amplify this DNA segment with primer pair Tac F/R (lanes 5 to 8).

Activation Assay:

Article Title: Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Article Snippet: The reaction mixture contained 5 µL of 5× RT-PCR buffer (including 2.5 mM MgCl2 ), 0.4 mM dNTPs, 0.5 µM of each of the four primers (synthesized in Bioneer, Korea), 1 µL of the enzyme mix, and 5 µL of RNA in a final volume of 25 µL. .. The RT-PCR conditions were as follows: a reverse transcription step at 50℃ for 30 min, reverse transcriptase inactivation and initial PCR activation at 95℃ for 15 min; 35 cycles of denaturation at 94℃ for 20 seconds, annealing at 55℃ for 20 seconds, and extension at 72℃ for 30 seconds; and a final elongation step at 72℃ for 10 min. T3000 Thermocycler (Biometra, Germany) was used for the RT-PCR.

Virus Isolation Assay:

Article Title: Occurrence of novel GII.17 and GII.21 norovirus variants in the coastal environment of South Korea in 2015
Article Snippet: A final extension was performed at 72°C for 7 min. For virus isolation process control in clams (control murine NoV RNA from digestive gland tissue), primers and RT-PCR conditions followed a previous description [ ]. .. The first PCR products (5 μL) were mixed with 10× buffer, 4 μL of 2.5 mM dNTPs, 50 μM forward/reverse primers (GIIF3M/GIIRIM for region C, GIIPF750M/GIIRIM for the ORF1-ORF2 junction, or GII.F3M/GIICR1450 for the nearly complete VP1; ) [ ], and 5 U of Top DNA polymerase (Bioneer, Daejeon, South Korea).

CTG Assay:

Article Title: SLC11A1 polymorphisms and host susceptibility to cutaneous leishmaniasis in Pakistan
Article Snippet: .. The reaction contained 200 ng of genomic DNA, 0.4 μM of each forward outer primer (5′-CTG CGG AAG CTG GAA GCT TTT TTT GGA C-3′) and T-allele specific reverse inner primer (5′-GAG GAA TGA TCT TGG GAG GTC CAC AGT TGA-3′), 0.2 μM of reverse outer primer (5′-CAC ATA CTG CAG GGA GGA GCC TGG TCA G-3′), 200 μM dNTPs each, 1.2 mM MgCl2 , 1× PCR buffer (400 mM KCl and 100 mM Tris-HCl, pH 9.0) and 1 unit of Taq DNA polymerase (Bioneer). .. Amplification was performed using Multi Block System 0.2S (ThermoHybaid) with the following conditions: initial denaturation at 94 °C for 4 min, followed by 35 cycles comprising denaturation at 94 °C for 30 s, annealing at 56 °C for 35 s and extension at 72 °C for 30 s. The last cycle was followed by a final extension at 72 °C for 7 min.

Staining:

Article Title: Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Article Snippet: The reaction mixture contained 5 µL of 5× RT-PCR buffer (including 2.5 mM MgCl2 ), 0.4 mM dNTPs, 0.5 µM of each of the four primers (synthesized in Bioneer, Korea), 1 µL of the enzyme mix, and 5 µL of RNA in a final volume of 25 µL. .. The amplicons were separated using electrophoresis in 1.5% agarose gels and stained with ethidium bromide.

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    Bioneer Corporation dntp
    Dntp, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 98/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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