Structured Review

TaKaRa dntps takara bio inc
Dntps Takara Bio Inc, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntps takara bio inc/product/TaKaRa
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dntps takara bio inc - by Bioz Stars, 2020-04
94/100 stars

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extaq polymerase takara bio

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Amplification:

Article Title: Schwann cell-specific Dp116 is expressed in glioblastoma cells, revealing two novel DMD gene splicing patterns
Article Snippet: .. PCR amplification was performed in a total volume of 10 μl, containing 1 μl of cDNA, 1 μl of 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U of ExTaq polymerase (Takara Bio, Inc.), 500 nM of each primer, and 250 μM dNTPs (Takara Bio, Inc.). .. Amplification was performed on a Mastercycler Gradient PCR machine (Eppendorf, Hamburg, Germany), with each consisting of an initial denaturation at 94 °C for 3 min, followed by 30 cycles of denaturation at 94 °C for 0.5 min, annealing at 60 °C for 0.5 min, and extension at 72 °C for 1.5 min. Amplified PCR products were electrophoresed on 2% agarose gels.

Synthesized:

Article Title: Schwann cell-specific Dp116 is expressed in glioblastoma cells, revealing two novel DMD gene splicing patterns
Article Snippet: Human total RNA from skeletal muscle was obtained from a human total RNA Master Panel II (Clontech Laboratories, Inc., Mountain View, CA, USA). cDNA was synthesized from 0.5 μg of each total RNA using random primers, as described [ ]. .. PCR amplification was performed in a total volume of 10 μl, containing 1 μl of cDNA, 1 μl of 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U of ExTaq polymerase (Takara Bio, Inc.), 500 nM of each primer, and 250 μM dNTPs (Takara Bio, Inc.).

Isolation:

Article Title: Schwann cell-specific Dp116 is expressed in glioblastoma cells, revealing two novel DMD gene splicing patterns
Article Snippet: RNA was extracted from these cells using High Pure RNA isolation kits (Roche Diagnostics). .. PCR amplification was performed in a total volume of 10 μl, containing 1 μl of cDNA, 1 μl of 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U of ExTaq polymerase (Takara Bio, Inc.), 500 nM of each primer, and 250 μM dNTPs (Takara Bio, Inc.).

Concentration Assay:

Article Title: Schwann cell-specific Dp116 is expressed in glioblastoma cells, revealing two novel DMD gene splicing patterns
Article Snippet: The integrity and concentration of these cDNA preparations were assessed by amplifying the mRNA of the house keeping gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH ), as described [ ]. .. PCR amplification was performed in a total volume of 10 μl, containing 1 μl of cDNA, 1 μl of 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U of ExTaq polymerase (Takara Bio, Inc.), 500 nM of each primer, and 250 μM dNTPs (Takara Bio, Inc.).

Polymerase Chain Reaction:

Article Title: Schwann cell-specific Dp116 is expressed in glioblastoma cells, revealing two novel DMD gene splicing patterns
Article Snippet: .. PCR amplification was performed in a total volume of 10 μl, containing 1 μl of cDNA, 1 μl of 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U of ExTaq polymerase (Takara Bio, Inc.), 500 nM of each primer, and 250 μM dNTPs (Takara Bio, Inc.). .. Amplification was performed on a Mastercycler Gradient PCR machine (Eppendorf, Hamburg, Germany), with each consisting of an initial denaturation at 94 °C for 3 min, followed by 30 cycles of denaturation at 94 °C for 0.5 min, annealing at 60 °C for 0.5 min, and extension at 72 °C for 1.5 min. Amplified PCR products were electrophoresed on 2% agarose gels.

Plasmid Preparation:

Article Title: Schwann cell-specific Dp116 is expressed in glioblastoma cells, revealing two novel DMD gene splicing patterns
Article Snippet: PCR amplification was performed in a total volume of 10 μl, containing 1 μl of cDNA, 1 μl of 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U of ExTaq polymerase (Takara Bio, Inc.), 500 nM of each primer, and 250 μM dNTPs (Takara Bio, Inc.). .. To examine the exon structure of the full-length transcript, the amplified product was subcloned into the pT7 blue T vector (Novagen, Inc., San Diego, CA, USA) and sequenced as described before [ ].

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  • 92
    TaKaRa deoxy ribonucleoside triphosphate dntp
    Deoxy Ribonucleoside Triphosphate Dntp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxy ribonucleoside triphosphate dntp/product/TaKaRa
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    deoxy ribonucleoside triphosphate dntp - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    dntp  (TaKaRa)
    99
    TaKaRa dntp
    Dntp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp/product/TaKaRa
    Average 99 stars, based on 1675 article reviews
    Price from $9.99 to $1999.99
    dntp - by Bioz Stars, 2020-04
    99/100 stars
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