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fluidigm dntpack s
Dntpack S, supplied by fluidigm, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntpack s/product/fluidigm
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dntpack s - by Bioz Stars, 2021-06
86/100 stars

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Polymerase Chain Reaction:

Article Title: Single-cell and single-variant resolution analysis of clonal evolution in human liver cancer
Article Snippet: WGA product from each single cell was used as PCR template for 5 parallel PCR reactions with ~10 primer pairs in each reaction. .. PCR cycling program was adopted from User Guide for Access Array System (Fluidigm, 100-3770) for even amplification of multiple targets. .. For each single cell, PCR products from the 5 reactions were mixed for library preparation using FastStart High Fidelity PCR System dNTPack (Roche, 3553400001) and Nextera XT Index Kit (illumina, FC-131-2001).

Article Title: DNA methylation clocks as a predictor for ageing and age estimation in naked mole-rats, Heterocephalus glaber
Article Snippet: DNA was bisulfite converted in a 96 well plate format using the EZ-96 DNA Methylation™ Kit (Zymo, Cat. D5003). .. Target amplification was performed using the FastStart High Fidelity PCR System, dNTPack (Sigma-Aldrich, Cat. 4738284001) in the 48.48 layout on the Fluidigm® C1 system (Fluidigm®, USA), a microfluidics platform. .. Library preparation was performed using the same kit including 4 μl of Access Array Barcode Library Primer and 1 μl of PCR product diluted 1:100.

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: In the serial dilution validation experiment, Q5 enzyme was used as described above, using the same STR plasmids as templates in three concentrations: 1 ng/μl (also used for the enzyme comparison experiment), 10−2 ng/μl and 10−4 ng/μl. .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR dataThe high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

Amplification:

Article Title: Single-cell and single-variant resolution analysis of clonal evolution in human liver cancer
Article Snippet: WGA product from each single cell was used as PCR template for 5 parallel PCR reactions with ~10 primer pairs in each reaction. .. PCR cycling program was adopted from User Guide for Access Array System (Fluidigm, 100-3770) for even amplification of multiple targets. .. For each single cell, PCR products from the 5 reactions were mixed for library preparation using FastStart High Fidelity PCR System dNTPack (Roche, 3553400001) and Nextera XT Index Kit (illumina, FC-131-2001).

Article Title: DNA methylation clocks as a predictor for ageing and age estimation in naked mole-rats, Heterocephalus glaber
Article Snippet: DNA was bisulfite converted in a 96 well plate format using the EZ-96 DNA Methylation™ Kit (Zymo, Cat. D5003). .. Target amplification was performed using the FastStart High Fidelity PCR System, dNTPack (Sigma-Aldrich, Cat. 4738284001) in the 48.48 layout on the Fluidigm® C1 system (Fluidigm®, USA), a microfluidics platform. .. Library preparation was performed using the same kit including 4 μl of Access Array Barcode Library Primer and 1 μl of PCR product diluted 1:100.

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: In the serial dilution validation experiment, Q5 enzyme was used as described above, using the same STR plasmids as templates in three concentrations: 1 ng/μl (also used for the enzyme comparison experiment), 10−2 ng/μl and 10−4 ng/μl. .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR dataThe high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

Activation Assay:

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: In the serial dilution validation experiment, Q5 enzyme was used as described above, using the same STR plasmids as templates in three concentrations: 1 ng/μl (also used for the enzyme comparison experiment), 10−2 ng/μl and 10−4 ng/μl. .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR dataThe high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

Chromatin Immunoprecipitation:

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: In the serial dilution validation experiment, Q5 enzyme was used as described above, using the same STR plasmids as templates in three concentrations: 1 ng/μl (also used for the enzyme comparison experiment), 10−2 ng/μl and 10−4 ng/μl. .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR dataThe high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

Concentration Assay:

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: In the serial dilution validation experiment, Q5 enzyme was used as described above, using the same STR plasmids as templates in three concentrations: 1 ng/μl (also used for the enzyme comparison experiment), 10−2 ng/μl and 10−4 ng/μl. .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR dataThe high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

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    fluidigm dntpack s
    Dntpack S, supplied by fluidigm, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntpack s/product/fluidigm
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dntpack s - by Bioz Stars, 2021-06
    86/100 stars
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