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Roche dntpack roche
Dntpack Roche, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntpack roche/product/Roche
Average 95 stars, based on 2 article reviews
Price from $9.99 to $1999.99
dntpack roche - by Bioz Stars, 2020-04
95/100 stars

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pcr
faststart high fidelity pcr system

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Related Articles

Amplification:

Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
Article Snippet: .. In the first PCR, 10 μl of cDNA were amplified using the FastStart High Fidelity PCR System, dNTPack (Roche) and the primers described in of the web extra material, to reach a single molecule covering the whole capsid coding region and a fragment of 2A ( ). .. Ten nested-PCRs were performed to amplify each of ten fragments, using the same kit but with 5 μl of DNA from the first-round PCR as template.

Agarose Gel Electrophoresis:

Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
Article Snippet: In the first PCR, 10 μl of cDNA were amplified using the FastStart High Fidelity PCR System, dNTPack (Roche) and the primers described in of the web extra material, to reach a single molecule covering the whole capsid coding region and a fragment of 2A ( ). .. Nested-PCR products were purified from agarose gel bands (QIAquick Gel Extraction Kit, QIAgen, CA, USA), quantified (Qubit™ dsDNA BR Assay Kit, Invitrogene, ThermoFisher), and tested for quality (Bioanalyzer DNA 1000, Agilent, CA, USA).

Quantitative RT-PCR:

Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
Article Snippet: Viral loads were determined as previously described [ ], using five μl of the extracted RNA and the RNA UltraSense™ One-Step Quantitative RT-PCR System (Invitrogen, ThermoFisher Scientific), and were expressed as genome copies/ml of serum. .. In the first PCR, 10 μl of cDNA were amplified using the FastStart High Fidelity PCR System, dNTPack (Roche) and the primers described in of the web extra material, to reach a single molecule covering the whole capsid coding region and a fragment of 2A ( ).

Purification:

Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
Article Snippet: In the first PCR, 10 μl of cDNA were amplified using the FastStart High Fidelity PCR System, dNTPack (Roche) and the primers described in of the web extra material, to reach a single molecule covering the whole capsid coding region and a fragment of 2A ( ). .. Nested-PCR products were purified from agarose gel bands (QIAquick Gel Extraction Kit, QIAgen, CA, USA), quantified (Qubit™ dsDNA BR Assay Kit, Invitrogene, ThermoFisher), and tested for quality (Bioanalyzer DNA 1000, Agilent, CA, USA).

Polymerase Chain Reaction:

Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
Article Snippet: .. In the first PCR, 10 μl of cDNA were amplified using the FastStart High Fidelity PCR System, dNTPack (Roche) and the primers described in of the web extra material, to reach a single molecule covering the whole capsid coding region and a fragment of 2A ( ). .. Ten nested-PCRs were performed to amplify each of ten fragments, using the same kit but with 5 μl of DNA from the first-round PCR as template.

Sequencing:

Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
Article Snippet: Consensus sequences were obtained using the ABI Prism® Big Dye™ Terminator Cycle Sequencing Ready Reaction Kit v3·1 (Applied Biosystems, CA, USA) and the ABI Prism 3700 automatic sequencer (Applied Biosystems). .. In the first PCR, 10 μl of cDNA were amplified using the FastStart High Fidelity PCR System, dNTPack (Roche) and the primers described in of the web extra material, to reach a single molecule covering the whole capsid coding region and a fragment of 2A ( ).

Gel Extraction:

Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
Article Snippet: In the first PCR, 10 μl of cDNA were amplified using the FastStart High Fidelity PCR System, dNTPack (Roche) and the primers described in of the web extra material, to reach a single molecule covering the whole capsid coding region and a fragment of 2A ( ). .. Nested-PCR products were purified from agarose gel bands (QIAquick Gel Extraction Kit, QIAgen, CA, USA), quantified (Qubit™ dsDNA BR Assay Kit, Invitrogene, ThermoFisher), and tested for quality (Bioanalyzer DNA 1000, Agilent, CA, USA).

Nested PCR:

Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
Article Snippet: In the first PCR, 10 μl of cDNA were amplified using the FastStart High Fidelity PCR System, dNTPack (Roche) and the primers described in of the web extra material, to reach a single molecule covering the whole capsid coding region and a fragment of 2A ( ). .. Nested-PCR products were purified from agarose gel bands (QIAquick Gel Extraction Kit, QIAgen, CA, USA), quantified (Qubit™ dsDNA BR Assay Kit, Invitrogene, ThermoFisher), and tested for quality (Bioanalyzer DNA 1000, Agilent, CA, USA).

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    Roche dntpack roche
    Dntpack Roche, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntpack roche/product/Roche
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dntpack roche - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

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