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Euromedex dntp
Dntp, supplied by Euromedex, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntp/product/Euromedex
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
dntp - by Bioz Stars, 2020-04
92/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Deciphering the Anti-Aflatoxinogenic Properties of Eugenol Using a Large-Scale q-PCR Approach
Article Snippet: The quality of RNA was verified by gel electrophoresis (1.2% agarose) and concentrations were measured using a NanoDrop ND1000 (Labtech, Palaiseau, France). .. Reverse transcription took place in a 20 μL reaction mixture containing 10 μL of RNA, 200 U of RevertAid Reverse transcriptase, 4 μL of 5× Reaction Buffer, 20 U of RNase inhibitor (Thermo Scientific, Illkirch, France), 2 μL of 10 mM dNTP (Euromedex, Souffelweyersheim, France), 1 μL of sterile water, and 1 μL of oligo (dT) Bys 3' Primer: (5'-GCTGTCAACGATACGCTATAACGGCATGACAGTGTTTTTTTTTTTTTTT-3').

Transfection:

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: For tranfection/infection competition experiments, 108 293 T cells were transfected by mixing 0.5 µg plasmid and TransIT-LT1 reagent (Mirus), according to the manufacturer’s instructions. .. The Uni12 primer (5′-AGC AAA AGC AGG-3′; 200 ng) was annealed to the viral RNA (8.5 µL) for 5 min at 70 °C, and then cDNA synthesis was performed with 11 units M-MuLV RT (Euromedex) and 1 mM dNTP (Euromedex) for 1 h at 42 °C in a final volume of 20 µL.

Amplification:

Article Title: Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses
Article Snippet: After amplification on MDCK cells in 48-well tissue-culture plates, vRNA was extracted from the culture supernatant using the RNeasy Mini kit (Qiagen). .. To identify gene origin (MO and EN), PCR was performed on 5 µL of cDNA in the presence of 200 ng of each primer, 20 mmol of dNTP (Euromedex), and 1.25 units of GoTaq polymerase (Promega) for 30 cycles of 1 min at 94 °C, 1 min at 58 °C or 60 °C, and 1 min at 72 °C ( ).

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: At 20 hours postinfection (h.p.i.), supernatants were harvested, and individual viruses were purified from plaques as described earlier and amplified on MDCK cells. .. The Uni12 primer (5′-AGC AAA AGC AGG-3′; 200 ng) was annealed to the viral RNA (8.5 µL) for 5 min at 70 °C, and then cDNA synthesis was performed with 11 units M-MuLV RT (Euromedex) and 1 mM dNTP (Euromedex) for 1 h at 42 °C in a final volume of 20 µL.

Article Title: Structure of faustovirus, a large dsDNA virus
Article Snippet: Primers for PCR amplification and sequencing ( ) were designed using PrimerQuest in the area containing the capsid sequences predicted by the preliminary bioinformatic study ( ). .. The RT reaction was performed in the automated thermal cycler PTC-200 (MJ Research) as follows: 25 °C for 10 min, 42 °C for 60 min, and 85 °C for 5 min. PCRs were performed with a PTC-200 automated thermal cycler (MJ Research) in a final volume of 25 µL of PCR mixture containing 2.5 µL of 10× buffer (Qiagen), 0.5 µL of each primer (10 pM; Eurogentec), 2.5 µL of each dNTP at 2 mM (euromedex), 1 µL of MgCl2 at 1.5 mM (Qiagen), 0.25 µL of HotStar Taq polymerase (Qiagen), 13 µL of Dnase/Rnase-free distilled water (Gibco), and 5 µL of DNA template or cDNA template.

Agarose Gel Electrophoresis:

Article Title: Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses
Article Snippet: To identify gene origin (MO and EN), PCR was performed on 5 µL of cDNA in the presence of 200 ng of each primer, 20 mmol of dNTP (Euromedex), and 1.25 units of GoTaq polymerase (Promega) for 30 cycles of 1 min at 94 °C, 1 min at 58 °C or 60 °C, and 1 min at 72 °C ( ). .. The primers were designed to be specific for a targeted origin of HA, PB1, NP, NA, and NS gene with PfuUltra II DNA polymerase (Agilent Technologies) and enable amplification by PCR followed by direct identification of gene origin by 0.8% agarose gel electrophoresis.

Synthesized:

Article Title: Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses
Article Snippet: Then cDNA strands were synthesized with 10 units of M-MuLv RT (Euromedex) and 20 mmol of dNTP (Euromedex) in a final volume of 20 µL for1 h at 42 °C. .. To identify gene origin (MO and EN), PCR was performed on 5 µL of cDNA in the presence of 200 ng of each primer, 20 mmol of dNTP (Euromedex), and 1.25 units of GoTaq polymerase (Promega) for 30 cycles of 1 min at 94 °C, 1 min at 58 °C or 60 °C, and 1 min at 72 °C ( ).

Article Title: Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses
Article Snippet: .. Then cDNA strands were synthesized with 10 units of M-MuLv RT (Euromedex) and 20 mmol of dNTP (Euromedex) in a final volume of 20 µL for1 h at 42 °C. .. To identify gene origin (MO and EN), PCR was performed on 5 µL of cDNA in the presence of 200 ng of each primer, 20 mmol of dNTP (Euromedex), and 1.25 units of GoTaq polymerase (Promega) for 30 cycles of 1 min at 94 °C, 1 min at 58 °C or 60 °C, and 1 min at 72 °C ( ).

Mutagenesis:

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: Six hours posttransfection, cells were infected with WT or mutant virus at a m.o.i. of 2. .. The Uni12 primer (5′-AGC AAA AGC AGG-3′; 200 ng) was annealed to the viral RNA (8.5 µL) for 5 min at 70 °C, and then cDNA synthesis was performed with 11 units M-MuLV RT (Euromedex) and 1 mM dNTP (Euromedex) for 1 h at 42 °C in a final volume of 20 µL.

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: WT and mutant segments 2 and 8 were identified by PCR, using specific primer pairs. .. PCR reactions contained 5 µL cDNA, 200 ng each primer, 0.4 mM dNTP (Euromedex), and 1.25 unit GoTaq polymerase (Promega).

Isolation:

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: To determine the genotype of the viruses isolated from plaques, viral RNA was extracted using a RNeasy Mini kit (Qiagen), as described earlier. .. The Uni12 primer (5′-AGC AAA AGC AGG-3′; 200 ng) was annealed to the viral RNA (8.5 µL) for 5 min at 70 °C, and then cDNA synthesis was performed with 11 units M-MuLV RT (Euromedex) and 1 mM dNTP (Euromedex) for 1 h at 42 °C in a final volume of 20 µL.

Article Title: Deciphering the Anti-Aflatoxinogenic Properties of Eugenol Using a Large-Scale q-PCR Approach
Article Snippet: Paragraph title: 5.4. Isolation of Fungal RNA and Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) ... Reverse transcription took place in a 20 μL reaction mixture containing 10 μL of RNA, 200 U of RevertAid Reverse transcriptase, 4 μL of 5× Reaction Buffer, 20 U of RNase inhibitor (Thermo Scientific, Illkirch, France), 2 μL of 10 mM dNTP (Euromedex, Souffelweyersheim, France), 1 μL of sterile water, and 1 μL of oligo (dT) Bys 3' Primer: (5'-GCTGTCAACGATACGCTATAACGGCATGACAGTGTTTTTTTTTTTTTTT-3').

Polymerase Chain Reaction:

Article Title: Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses
Article Snippet: .. To identify gene origin (MO and EN), PCR was performed on 5 µL of cDNA in the presence of 200 ng of each primer, 20 mmol of dNTP (Euromedex), and 1.25 units of GoTaq polymerase (Promega) for 30 cycles of 1 min at 94 °C, 1 min at 58 °C or 60 °C, and 1 min at 72 °C ( ). .. The primers were designed to be specific for a targeted origin of HA, PB1, NP, NA, and NS gene with PfuUltra II DNA polymerase (Agilent Technologies) and enable amplification by PCR followed by direct identification of gene origin by 0.8% agarose gel electrophoresis.

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: The Uni12 primer (5′-AGC AAA AGC AGG-3′; 200 ng) was annealed to the viral RNA (8.5 µL) for 5 min at 70 °C, and then cDNA synthesis was performed with 11 units M-MuLV RT (Euromedex) and 1 mM dNTP (Euromedex) for 1 h at 42 °C in a final volume of 20 µL. .. WT and mutant segments 2 and 8 were identified by PCR, using specific primer pairs.

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: .. PCR reactions contained 5 µL cDNA, 200 ng each primer, 0.4 mM dNTP (Euromedex), and 1.25 unit GoTaq polymerase (Promega). ..

Article Title: Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses
Article Snippet: Paragraph title: PCR Screening of Gene Origins. ... Then cDNA strands were synthesized with 10 units of M-MuLv RT (Euromedex) and 20 mmol of dNTP (Euromedex) in a final volume of 20 µL for1 h at 42 °C.

Article Title: Structure of faustovirus, a large dsDNA virus
Article Snippet: .. The RT reaction was performed in the automated thermal cycler PTC-200 (MJ Research) as follows: 25 °C for 10 min, 42 °C for 60 min, and 85 °C for 5 min. PCRs were performed with a PTC-200 automated thermal cycler (MJ Research) in a final volume of 25 µL of PCR mixture containing 2.5 µL of 10× buffer (Qiagen), 0.5 µL of each primer (10 pM; Eurogentec), 2.5 µL of each dNTP at 2 mM (euromedex), 1 µL of MgCl2 at 1.5 mM (Qiagen), 0.25 µL of HotStar Taq polymerase (Qiagen), 13 µL of Dnase/Rnase-free distilled water (Gibco), and 5 µL of DNA template or cDNA template. .. Negative controls consisting of the PCR mixture without DNA template were added in each PCR run.

Infection:

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: Six hours posttransfection, cells were infected with WT or mutant virus at a m.o.i. of 2. .. The Uni12 primer (5′-AGC AAA AGC AGG-3′; 200 ng) was annealed to the viral RNA (8.5 µL) for 5 min at 70 °C, and then cDNA synthesis was performed with 11 units M-MuLV RT (Euromedex) and 1 mM dNTP (Euromedex) for 1 h at 42 °C in a final volume of 20 µL.

Purification:

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: At 20 hours postinfection (h.p.i.), supernatants were harvested, and individual viruses were purified from plaques as described earlier and amplified on MDCK cells. .. The Uni12 primer (5′-AGC AAA AGC AGG-3′; 200 ng) was annealed to the viral RNA (8.5 µL) for 5 min at 70 °C, and then cDNA synthesis was performed with 11 units M-MuLV RT (Euromedex) and 1 mM dNTP (Euromedex) for 1 h at 42 °C in a final volume of 20 µL.

Article Title: Structure of faustovirus, a large dsDNA virus
Article Snippet: The RT reaction was performed in the automated thermal cycler PTC-200 (MJ Research) as follows: 25 °C for 10 min, 42 °C for 60 min, and 85 °C for 5 min. PCRs were performed with a PTC-200 automated thermal cycler (MJ Research) in a final volume of 25 µL of PCR mixture containing 2.5 µL of 10× buffer (Qiagen), 0.5 µL of each primer (10 pM; Eurogentec), 2.5 µL of each dNTP at 2 mM (euromedex), 1 µL of MgCl2 at 1.5 mM (Qiagen), 0.25 µL of HotStar Taq polymerase (Qiagen), 13 µL of Dnase/Rnase-free distilled water (Gibco), and 5 µL of DNA template or cDNA template. .. PCR products were purified with the PCR filter plate Millipore NucleoFast 96 PCR kit as recommended by the manufacturer (Macherey–Nagel).

Article Title: Deciphering the Anti-Aflatoxinogenic Properties of Eugenol Using a Large-Scale q-PCR Approach
Article Snippet: The RNA was purified as recommended by the manufacturer from 100 mg of mycelium using a Qiagen RNeasy PlusMinikit (Qiagen, Hilden, Germany) and including a gDNA eliminator column. .. Reverse transcription took place in a 20 μL reaction mixture containing 10 μL of RNA, 200 U of RevertAid Reverse transcriptase, 4 μL of 5× Reaction Buffer, 20 U of RNase inhibitor (Thermo Scientific, Illkirch, France), 2 μL of 10 mM dNTP (Euromedex, Souffelweyersheim, France), 1 μL of sterile water, and 1 μL of oligo (dT) Bys 3' Primer: (5'-GCTGTCAACGATACGCTATAACGGCATGACAGTGTTTTTTTTTTTTTTT-3').

Reverse Transcription Polymerase Chain Reaction:

Article Title: Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses
Article Snippet: RT-PCR started with the denaturation of 8.5 µL of vRNA in the presence of 200 ng of the Uni12 primer (5′-AGC AAA AGC AGG-3′) for 5 min at 70 °C. .. To identify gene origin (MO and EN), PCR was performed on 5 µL of cDNA in the presence of 200 ng of each primer, 20 mmol of dNTP (Euromedex), and 1.25 units of GoTaq polymerase (Promega) for 30 cycles of 1 min at 94 °C, 1 min at 58 °C or 60 °C, and 1 min at 72 °C ( ).

Article Title: Deciphering the Anti-Aflatoxinogenic Properties of Eugenol Using a Large-Scale q-PCR Approach
Article Snippet: Paragraph title: 5.4. Isolation of Fungal RNA and Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) ... Reverse transcription took place in a 20 μL reaction mixture containing 10 μL of RNA, 200 U of RevertAid Reverse transcriptase, 4 μL of 5× Reaction Buffer, 20 U of RNase inhibitor (Thermo Scientific, Illkirch, France), 2 μL of 10 mM dNTP (Euromedex, Souffelweyersheim, France), 1 μL of sterile water, and 1 μL of oligo (dT) Bys 3' Primer: (5'-GCTGTCAACGATACGCTATAACGGCATGACAGTGTTTTTTTTTTTTTTT-3').

Incubation:

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: One hour later, cells were washed and incubated at 37 °C with EMEM supplemented with 1 μg/mL TPCK-trypsin. .. The Uni12 primer (5′-AGC AAA AGC AGG-3′; 200 ng) was annealed to the viral RNA (8.5 µL) for 5 min at 70 °C, and then cDNA synthesis was performed with 11 units M-MuLV RT (Euromedex) and 1 mM dNTP (Euromedex) for 1 h at 42 °C in a final volume of 20 µL.

Article Title: Deciphering the Anti-Aflatoxinogenic Properties of Eugenol Using a Large-Scale q-PCR Approach
Article Snippet: Isolation of Fungal RNA and Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) At the end of incubation, mycelia were separated from the medium and ground up under liquid nitrogen. .. Reverse transcription took place in a 20 μL reaction mixture containing 10 μL of RNA, 200 U of RevertAid Reverse transcriptase, 4 μL of 5× Reaction Buffer, 20 U of RNase inhibitor (Thermo Scientific, Illkirch, France), 2 μL of 10 mM dNTP (Euromedex, Souffelweyersheim, France), 1 μL of sterile water, and 1 μL of oligo (dT) Bys 3' Primer: (5'-GCTGTCAACGATACGCTATAACGGCATGACAGTGTTTTTTTTTTTTTTT-3').

Plasmid Preparation:

Article Title: A functional sequence-specific interaction between influenza A virus genomic RNA segments
Article Snippet: For tranfection/infection competition experiments, 108 293 T cells were transfected by mixing 0.5 µg plasmid and TransIT-LT1 reagent (Mirus), according to the manufacturer’s instructions. .. The Uni12 primer (5′-AGC AAA AGC AGG-3′; 200 ng) was annealed to the viral RNA (8.5 µL) for 5 min at 70 °C, and then cDNA synthesis was performed with 11 units M-MuLV RT (Euromedex) and 1 mM dNTP (Euromedex) for 1 h at 42 °C in a final volume of 20 µL.

Sequencing:

Article Title: Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses
Article Snippet: To identify gene origin (MO and EN), PCR was performed on 5 µL of cDNA in the presence of 200 ng of each primer, 20 mmol of dNTP (Euromedex), and 1.25 units of GoTaq polymerase (Promega) for 30 cycles of 1 min at 94 °C, 1 min at 58 °C or 60 °C, and 1 min at 72 °C ( ). .. MMO , MC3MO , and MC5MO screenings were realized by sequencing.

Article Title: Structure of faustovirus, a large dsDNA virus
Article Snippet: Primers for PCR amplification and sequencing ( ) were designed using PrimerQuest in the area containing the capsid sequences predicted by the preliminary bioinformatic study ( ). .. The RT reaction was performed in the automated thermal cycler PTC-200 (MJ Research) as follows: 25 °C for 10 min, 42 °C for 60 min, and 85 °C for 5 min. PCRs were performed with a PTC-200 automated thermal cycler (MJ Research) in a final volume of 25 µL of PCR mixture containing 2.5 µL of 10× buffer (Qiagen), 0.5 µL of each primer (10 pM; Eurogentec), 2.5 µL of each dNTP at 2 mM (euromedex), 1 µL of MgCl2 at 1.5 mM (Qiagen), 0.25 µL of HotStar Taq polymerase (Qiagen), 13 µL of Dnase/Rnase-free distilled water (Gibco), and 5 µL of DNA template or cDNA template.

Concentration Assay:

Article Title: Deciphering the Anti-Aflatoxinogenic Properties of Eugenol Using a Large-Scale q-PCR Approach
Article Snippet: The A260 /A280 ratio was measured [ ], and each sample was adjusted to a final RNA concentration of 300 ng/μL. .. Reverse transcription took place in a 20 μL reaction mixture containing 10 μL of RNA, 200 U of RevertAid Reverse transcriptase, 4 μL of 5× Reaction Buffer, 20 U of RNase inhibitor (Thermo Scientific, Illkirch, France), 2 μL of 10 mM dNTP (Euromedex, Souffelweyersheim, France), 1 μL of sterile water, and 1 μL of oligo (dT) Bys 3' Primer: (5'-GCTGTCAACGATACGCTATAACGGCATGACAGTGTTTTTTTTTTTTTTT-3').

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  • 90
    Euromedex dntp mixture
    Dntp Mixture, supplied by Euromedex, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp mixture/product/Euromedex
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dntp mixture - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    92
    Euromedex dntp
    Dntp, supplied by Euromedex, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp/product/Euromedex
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    dntp - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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