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Eppendorf AG dntp
Dntp, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dntp - by Bioz Stars, 2020-04
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DNA Extraction:

Article Title: Integrated Operational Taxonomic Units (IOTUs) in Echolocating Bats: A Bridge between Molecular and Traditional Taxonomy
Article Snippet: Paragraph title: DNA Extraction, PCR Conditions, DNA Sequencing and Alignment ... To amplify the 658 bp target region of coxI , we used primers VF1d (5′-TTCTCAACCAACCACAAR GAYATYGG-3′) and VR1d (5′-TAGACTTCTGGGTGGCCRAARAAYCA-3′) in a 20-µl PCR containing 1X MasterTaq buffer with 1.5 mM MgCl2 (Eppendorf AG, Hamburg, Germany), 0.2 mM of each dNTP, 1 µM of each primer, 1 U of MasterTaq DNA polymerase (Eppendorf AG, Hamburg, Germany) and 1–10 ng of template DNA.

Article Title: Desert Springs: Deep Phylogeographic Structure in an Ancient Endemic Crustacean (Phreatomerus latipes)
Article Snippet: Paragraph title: DNA Isolation and Amplification of Mitochondrial DNA ... Polymerase Chain Reaction (PCR) amplification of all sequences involved an initial cycle of denaturation at 95°C for 2 min, and 35 subsequent cycles of 94°C for 30 seconds (s), 50°C for 30 s and 72°C for 1 min. PCR was carried out in 25 µl reactions containing 10×Eppendorf Hotmaster® Taq Buffer (Eppendorf, Westbury, NY, USA) containing 2.5 mM Mg2+ , 2.5 mM of each dNTP, 5.0 µM of each primer, 0.1 units of Eppendorf Hotmaster® Taq Polymerase and ∼1 ng of DNA.

Article Title: Lack of Association Between Transforming Growth Factor Beta 1 -509C/T and +915G/C Polymorphisms and Chronic Hepatitis B in Iranian Patients
Article Snippet: Paragraph title: 3.2. DNA Extraction and Genotyping ... Each single reaction used 100 ng DNA, 2.5 µL buffer, 1.5 µM MgCl2 , 25 µM of each dNTP and 1.25 U Taq polymerase (Eppendorf AG, Hamburg, Germany).

Clone Assay:

Article Title: Screening and Transcriptional Analysis of Polyketide Synthases and Non-ribosomal Peptide Synthetases in Bacterial Strains From Krubera–Voronja Cave
Article Snippet: Amplification was carried out in 50 μL of reaction mixture containing PCR buffer with (NH4 )2 SO4 , 2 mM MgCl2 , 0.2 mM each dNTP, 0.25 μM of each primer, 1.25 U recombinant Taq DNA Polymerase, and 10 ng of bacterial genomic DNA in an Eppendorf Mastercycler EP Gradient (Eppendorf, Hamburg, Germany). .. PCR products of the correct size were purified using either the GeneJET PCR Purification Kit or the GeneJET Gel Extraction Kit (Thermo Fisher Scientific), and cloned into E. coli DH5α using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.

Article Title: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
Article Snippet: .. Polymerase chain reactions (final volumes 50 μl) containing 1× HiFi Buffer (Eppendorf, Westbury, NY), 0.2 mM each dNTP, 0.25 uM each primer, 50 ng of the template plasmids, and 2 U Triple Master DNA Polymerase (Eppendorf, Westbury, NY) were carried out to obtain the CHD7 novel isoform coding sequences from clones M1, M2 and M3. ..

Article Title: Smenamides A and B, Chlorinated Peptide/Polyketide Hybrids Containing a Dolapyrrolidinone Unit from the Caribbean Sponge Smenospongia aurea. Evaluation of Their Role as Leads in Antitumor Drug Research
Article Snippet: The reaction mixture (25 µL) contained: 14.65 µL H2 O, 0.5 µL DMSO, 1.5 µL dNTP (10 mM), 2.5 µL Taq buffer advanced (Eppendorf, Hamburg, Germany), 2.5 µL forward primer CYA106F (10 µM), 2.5 µL reverse primer CYA781R(a) or CYA781R(b) (10 µM), 0.35 µL RBC Taq DNA polymerase (5 U/µL, RBC Bioscience, New Taipei City, Taiwan), 0.5 µL DNA. .. The PCR products of the expected size (~670 bp) were purified from the agarose gel using QIAquick gel ex kit (Qiagen, Venlo, The Netherlands), subcloned via T/A cloning into pBluescriptII SK(+) (Stratagene California, La Jolla, CA, USA), and transferred to E. coli XL1 Blue MRF' (Stratagene California, La Jolla, CA, USA) electrocompetent cells.

Centrifugation:

Article Title: Expression of Connexin36 in Cone Pedicles and OFF-Cone Bipolar Cells of the Mouse Retina
Article Snippet: Subsequently, the tip of the pipette was broken into a PCR tube containing 20 U of RNase inhibitor (Rnasin; Promega, Madison, WI), and the sample (∼8 μl) was subjected to a brief centrifugation. .. This included 2 and 6 μl retinal and photoreceptor cDNA, respectively, 1× reaction buffer (Promega), 1.25 m m MgCl2 , 0.2 m m of each dNTP (Eppendorf), 0.8 μ m of each primer (MWG Biotech, Ebersberg, Germany), and 1 U of Taq polymerase (Promega).

Amplification:

Article Title: Screening and Transcriptional Analysis of Polyketide Synthases and Non-ribosomal Peptide Synthetases in Bacterial Strains From Krubera–Voronja Cave
Article Snippet: .. Amplification was carried out in 50 μL of reaction mixture containing PCR buffer with (NH4 )2 SO4 , 2 mM MgCl2 , 0.2 mM each dNTP, 0.25 μM of each primer, 1.25 U recombinant Taq DNA Polymerase, and 10 ng of bacterial genomic DNA in an Eppendorf Mastercycler EP Gradient (Eppendorf, Hamburg, Germany). .. Amplification conditions for each primer pair were selected according to the references listed in .

Article Title: Integrated Operational Taxonomic Units (IOTUs) in Echolocating Bats: A Bridge between Molecular and Traditional Taxonomy
Article Snippet: To amplify the 658 bp target region of coxI , we used primers VF1d (5′-TTCTCAACCAACCACAAR GAYATYGG-3′) and VR1d (5′-TAGACTTCTGGGTGGCCRAARAAYCA-3′) in a 20-µl PCR containing 1X MasterTaq buffer with 1.5 mM MgCl2 (Eppendorf AG, Hamburg, Germany), 0.2 mM of each dNTP, 1 µM of each primer, 1 U of MasterTaq DNA polymerase (Eppendorf AG, Hamburg, Germany) and 1–10 ng of template DNA. .. PCR products were gel-purified using the Perfectprep Gel Cleanup (Eppendorf AG, Hamburg, Germany) and sequenced directly on an ABI3730XL automated sequencer (Macrogen Inc., Korea) with both PCR amplification primers.

Article Title: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
Article Snippet: Cloning of the CHD7 CRA_e isoform coding sequence into a bicistronic lentiviral expression vector The CHD7 CRA_e isoform coding sequence from clones M1, M2 and M3 were amplified by PCR from pGEMT-M1, pGEM-M2 and pGEM-M3 vectors and sub-cloned into the p156RRLsinPPTCMVIRESPRE third generation transfer bicistronic lentiviral vector [ ] (heretofore referred to as pLV-EGFP). .. Polymerase chain reactions (final volumes 50 μl) containing 1× HiFi Buffer (Eppendorf, Westbury, NY), 0.2 mM each dNTP, 0.25 uM each primer, 50 ng of the template plasmids, and 2 U Triple Master DNA Polymerase (Eppendorf, Westbury, NY) were carried out to obtain the CHD7 novel isoform coding sequences from clones M1, M2 and M3.

Article Title: The role of Rhodotorula mucilaginosa in selected biological process of wild fish
Article Snippet: Cultures were incubated on the YEPD medium (1.0% yeast extract, 2.0%—peptone, 2.0%—glucose; Scharlau) at 22 °C for 24 h. Genetic differentiation analysis was carried out with the random amplified polymorphic DNA method (RAPD-PCR). .. The RAPD-PCR reaction was conducted in 25 μL of the reaction mixture containing the following: 500 mM KCl, 100 mM Tris–HCl (pH 8.3 at 25 °C), 1.25 mM MgCl2 , 0.3 mM dNTP, 20 pmol/μL of each starter, 1 U Taq DNA polymerase (Eppendorf), and 20 ng DNA template.

Article Title: Expression of Connexin36 in Cone Pedicles and OFF-Cone Bipolar Cells of the Mouse Retina
Article Snippet: Contaminating genomic DNA was digested with DNase I (Amplification Grade; Invitrogen, Carlsbad, CA) according to the protocol of the manufacturer. cDNA synthesis from 2–4 μg of retinal RNA and from the photoreceptor samples was performed in final volumes of 50 and 25 μl, respectively. .. This included 2 and 6 μl retinal and photoreceptor cDNA, respectively, 1× reaction buffer (Promega), 1.25 m m MgCl2 , 0.2 m m of each dNTP (Eppendorf), 0.8 μ m of each primer (MWG Biotech, Ebersberg, Germany), and 1 U of Taq polymerase (Promega).

Article Title: Smenamides A and B, Chlorinated Peptide/Polyketide Hybrids Containing a Dolapyrrolidinone Unit from the Caribbean Sponge Smenospongia aurea. Evaluation of Their Role as Leads in Antitumor Drug Research
Article Snippet: Paragraph title: 3.6. PCR Amplification and Clone Library Construction ... The reaction mixture (25 µL) contained: 14.65 µL H2 O, 0.5 µL DMSO, 1.5 µL dNTP (10 mM), 2.5 µL Taq buffer advanced (Eppendorf, Hamburg, Germany), 2.5 µL forward primer CYA106F (10 µM), 2.5 µL reverse primer CYA781R(a) or CYA781R(b) (10 µM), 0.35 µL RBC Taq DNA polymerase (5 U/µL, RBC Bioscience, New Taipei City, Taiwan), 0.5 µL DNA.

Article Title: Antimicrobial Activity and Genetic Profile of Enteroccoci Isolated from Hoopoes Uropygial Gland
Article Snippet: A 700-bp fragment of the 16S rRNA gene, which included variable regions V1 to V4, was amplified for representative strains of each RAPD genotype. .. The PCR was carried out using a 50 µl (total volume) mixture containing 5 µl of 10× Taq reaction buffer, 10 µl of 5× Taq Enhancer, 1.5 mM magnesium diacetate, each dNTP at a concentration of 400 µM, 0.4 µM primer WO1 (5′-AGAGTTTGATC[AC]TGGCTC-3′), 0.4 µM primer WO12 (5′-TACGCATTTCACC[GT]CTACA-3′), 1 U of Eppendorf Master Taq polymerase, and 1 µl of template DNA.

Article Title: Desert Springs: Deep Phylogeographic Structure in an Ancient Endemic Crustacean (Phreatomerus latipes)
Article Snippet: .. Polymerase Chain Reaction (PCR) amplification of all sequences involved an initial cycle of denaturation at 95°C for 2 min, and 35 subsequent cycles of 94°C for 30 seconds (s), 50°C for 30 s and 72°C for 1 min. PCR was carried out in 25 µl reactions containing 10×Eppendorf Hotmaster® Taq Buffer (Eppendorf, Westbury, NY, USA) containing 2.5 mM Mg2+ , 2.5 mM of each dNTP, 5.0 µM of each primer, 0.1 units of Eppendorf Hotmaster® Taq Polymerase and ∼1 ng of DNA. .. These PCR products were sequenced using the ABI PRISM Big Dye Terminator Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 3700 DNA analyzer.

Article Title: Traceability of Plant Diet Contents in Raw Cow Milk Samples
Article Snippet: .. Each PCR-based DNA amplification was performed in a total volume of 30 μL, in 1 × Taq Advanced buffer (Eppendorf), 2 mM MgCl2 , 200 μM of each dNTP, 1 μM of each Primer and 1 U of Taq DNA Polymerase (Eppendorf). ..

Article Title: A set of microsatellite markers with long core repeat optimized for grape (Vitis spp.) genotyping
Article Snippet: PCR primer testing Four genotypes, the Vitis vinifera cultivars Chardonnay and Cabernet Sauvignon, and the Vitis hybrids Bianca and 20/3, were used for a preliminary test of PCR amplification of the 94 markers initially selected. .. PCR reactions were carried in 10 μL using 200 μM each dNTP, 0.2 μM each primer, 10 ng genomic DNA, and 0.2 U of HotMaster Taq polymerase (Eppendorf).

Article Title: Native Bacterial Endophytes Promote Host Growth in a Species-Specific Manner; Phytohormone Manipulations Do Not Result in Common Growth Responses
Article Snippet: .. Amplification of 16S rDNA was performed in a 10 µl final volume containing 1 µl of total DNA, 10 µM of primer F27 ( 5′-AGAGTTTATCMTGGCTCAG-3′ ) and R1492 ( 5′-GRTACCTTGTTACGACTT-3′ ) , 10 mM of each dNTP, 5 mM MgCl2 and 0.05U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). ..

Agarose Gel Electrophoresis:

Article Title: The role of Rhodotorula mucilaginosa in selected biological process of wild fish
Article Snippet: The RAPD-PCR reaction was conducted in 25 μL of the reaction mixture containing the following: 500 mM KCl, 100 mM Tris–HCl (pH 8.3 at 25 °C), 1.25 mM MgCl2 , 0.3 mM dNTP, 20 pmol/μL of each starter, 1 U Taq DNA polymerase (Eppendorf), and 20 ng DNA template. .. Amplification products were separated electrophoretically in 2.0% agarose gel (Prona Agarose Plus) with ethidium bromide (0.5 μL/mL) (Bio-Rad, USA).

Article Title: Smenamides A and B, Chlorinated Peptide/Polyketide Hybrids Containing a Dolapyrrolidinone Unit from the Caribbean Sponge Smenospongia aurea. Evaluation of Their Role as Leads in Antitumor Drug Research
Article Snippet: The reaction mixture (25 µL) contained: 14.65 µL H2 O, 0.5 µL DMSO, 1.5 µL dNTP (10 mM), 2.5 µL Taq buffer advanced (Eppendorf, Hamburg, Germany), 2.5 µL forward primer CYA106F (10 µM), 2.5 µL reverse primer CYA781R(a) or CYA781R(b) (10 µM), 0.35 µL RBC Taq DNA polymerase (5 U/µL, RBC Bioscience, New Taipei City, Taiwan), 0.5 µL DNA. .. The PCR products of the expected size (~670 bp) were purified from the agarose gel using QIAquick gel ex kit (Qiagen, Venlo, The Netherlands), subcloned via T/A cloning into pBluescriptII SK(+) (Stratagene California, La Jolla, CA, USA), and transferred to E. coli XL1 Blue MRF' (Stratagene California, La Jolla, CA, USA) electrocompetent cells.

Polymerase Chain Reaction:

Article Title: Screening and Transcriptional Analysis of Polyketide Synthases and Non-ribosomal Peptide Synthetases in Bacterial Strains From Krubera–Voronja Cave
Article Snippet: .. Amplification was carried out in 50 μL of reaction mixture containing PCR buffer with (NH4 )2 SO4 , 2 mM MgCl2 , 0.2 mM each dNTP, 0.25 μM of each primer, 1.25 U recombinant Taq DNA Polymerase, and 10 ng of bacterial genomic DNA in an Eppendorf Mastercycler EP Gradient (Eppendorf, Hamburg, Germany). .. Amplification conditions for each primer pair were selected according to the references listed in .

Article Title: Integrated Operational Taxonomic Units (IOTUs) in Echolocating Bats: A Bridge between Molecular and Traditional Taxonomy
Article Snippet: .. To amplify the 658 bp target region of coxI , we used primers VF1d (5′-TTCTCAACCAACCACAAR GAYATYGG-3′) and VR1d (5′-TAGACTTCTGGGTGGCCRAARAAYCA-3′) in a 20-µl PCR containing 1X MasterTaq buffer with 1.5 mM MgCl2 (Eppendorf AG, Hamburg, Germany), 0.2 mM of each dNTP, 1 µM of each primer, 1 U of MasterTaq DNA polymerase (Eppendorf AG, Hamburg, Germany) and 1–10 ng of template DNA. ..

Article Title: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
Article Snippet: Cloning of the CHD7 CRA_e isoform coding sequence into a bicistronic lentiviral expression vector The CHD7 CRA_e isoform coding sequence from clones M1, M2 and M3 were amplified by PCR from pGEMT-M1, pGEM-M2 and pGEM-M3 vectors and sub-cloned into the p156RRLsinPPTCMVIRESPRE third generation transfer bicistronic lentiviral vector [ ] (heretofore referred to as pLV-EGFP). .. Polymerase chain reactions (final volumes 50 μl) containing 1× HiFi Buffer (Eppendorf, Westbury, NY), 0.2 mM each dNTP, 0.25 uM each primer, 50 ng of the template plasmids, and 2 U Triple Master DNA Polymerase (Eppendorf, Westbury, NY) were carried out to obtain the CHD7 novel isoform coding sequences from clones M1, M2 and M3.

Article Title: The role of Rhodotorula mucilaginosa in selected biological process of wild fish
Article Snippet: Species identity of selected strains (Rhodotorula mucilaginosa and Rhodotorula glutinis ) was confirmed with the PCR method, accordingly to White et al. ( ). .. The RAPD-PCR reaction was conducted in 25 μL of the reaction mixture containing the following: 500 mM KCl, 100 mM Tris–HCl (pH 8.3 at 25 °C), 1.25 mM MgCl2 , 0.3 mM dNTP, 20 pmol/μL of each starter, 1 U Taq DNA polymerase (Eppendorf), and 20 ng DNA template.

Article Title: Expression of Connexin36 in Cone Pedicles and OFF-Cone Bipolar Cells of the Mouse Retina
Article Snippet: PCR reactions were performed in a total volume of 25 μl. .. This included 2 and 6 μl retinal and photoreceptor cDNA, respectively, 1× reaction buffer (Promega), 1.25 m m MgCl2 , 0.2 m m of each dNTP (Eppendorf), 0.8 μ m of each primer (MWG Biotech, Ebersberg, Germany), and 1 U of Taq polymerase (Promega).

Article Title: Smenamides A and B, Chlorinated Peptide/Polyketide Hybrids Containing a Dolapyrrolidinone Unit from the Caribbean Sponge Smenospongia aurea. Evaluation of Their Role as Leads in Antitumor Drug Research
Article Snippet: Paragraph title: 3.6. PCR Amplification and Clone Library Construction ... The reaction mixture (25 µL) contained: 14.65 µL H2 O, 0.5 µL DMSO, 1.5 µL dNTP (10 mM), 2.5 µL Taq buffer advanced (Eppendorf, Hamburg, Germany), 2.5 µL forward primer CYA106F (10 µM), 2.5 µL reverse primer CYA781R(a) or CYA781R(b) (10 µM), 0.35 µL RBC Taq DNA polymerase (5 U/µL, RBC Bioscience, New Taipei City, Taiwan), 0.5 µL DNA.

Article Title: Antimicrobial Activity and Genetic Profile of Enteroccoci Isolated from Hoopoes Uropygial Gland
Article Snippet: .. The PCR was carried out using a 50 µl (total volume) mixture containing 5 µl of 10× Taq reaction buffer, 10 µl of 5× Taq Enhancer, 1.5 mM magnesium diacetate, each dNTP at a concentration of 400 µM, 0.4 µM primer WO1 (5′-AGAGTTTGATC[AC]TGGCTC-3′), 0.4 µM primer WO12 (5′-TACGCATTTCACC[GT]CTACA-3′), 1 U of Eppendorf Master Taq polymerase, and 1 µl of template DNA. .. The amplification program consisted of an initial denaturing step of 94°C for 4 min, followed by amplification using 30 cycles of 30 s at 94°C, 30 s at 50°C, and 60 s at 72°C and then a final extension at 72°C for 2 min. PCR products were purified with a Perfectprep gel cleanup kit (Eppendorf, Hamburg, Germany).

Article Title: Desert Springs: Deep Phylogeographic Structure in an Ancient Endemic Crustacean (Phreatomerus latipes)
Article Snippet: .. Polymerase Chain Reaction (PCR) amplification of all sequences involved an initial cycle of denaturation at 95°C for 2 min, and 35 subsequent cycles of 94°C for 30 seconds (s), 50°C for 30 s and 72°C for 1 min. PCR was carried out in 25 µl reactions containing 10×Eppendorf Hotmaster® Taq Buffer (Eppendorf, Westbury, NY, USA) containing 2.5 mM Mg2+ , 2.5 mM of each dNTP, 5.0 µM of each primer, 0.1 units of Eppendorf Hotmaster® Taq Polymerase and ∼1 ng of DNA. .. These PCR products were sequenced using the ABI PRISM Big Dye Terminator Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 3700 DNA analyzer.

Article Title: Lack of Association Between Transforming Growth Factor Beta 1 -509C/T and +915G/C Polymorphisms and Chronic Hepatitis B in Iranian Patients
Article Snippet: The total volume for a single PCR cycle of either polymorphism was 25 µL. .. Each single reaction used 100 ng DNA, 2.5 µL buffer, 1.5 µM MgCl2 , 25 µM of each dNTP and 1.25 U Taq polymerase (Eppendorf AG, Hamburg, Germany).

Article Title: Traceability of Plant Diet Contents in Raw Cow Milk Samples
Article Snippet: .. Each PCR-based DNA amplification was performed in a total volume of 30 μL, in 1 × Taq Advanced buffer (Eppendorf), 2 mM MgCl2 , 200 μM of each dNTP, 1 μM of each Primer and 1 U of Taq DNA Polymerase (Eppendorf). ..

Article Title: A set of microsatellite markers with long core repeat optimized for grape (Vitis spp.) genotyping
Article Snippet: .. PCR reactions were carried in 10 μL using 200 μM each dNTP, 0.2 μM each primer, 10 ng genomic DNA, and 0.2 U of HotMaster Taq polymerase (Eppendorf). ..

Isolation:

Article Title: The role of Rhodotorula mucilaginosa in selected biological process of wild fish
Article Snippet: Genomic DNA was isolated following the protocol of the QIAamp DNA Mini Kit (Qiagen) using lyticase (Sigma-Aldrich). .. The RAPD-PCR reaction was conducted in 25 μL of the reaction mixture containing the following: 500 mM KCl, 100 mM Tris–HCl (pH 8.3 at 25 °C), 1.25 mM MgCl2 , 0.3 mM dNTP, 20 pmol/μL of each starter, 1 U Taq DNA polymerase (Eppendorf), and 20 ng DNA template.

Article Title: Antimicrobial Activity and Genetic Profile of Enteroccoci Isolated from Hoopoes Uropygial Gland
Article Snippet: Identification of Bacterial Isolates We performed a preliminary identification of the isolates based on a few phenotypic traits for the colonies isolated from samples of 2003 and 2005 breeding seasons (N = 45). .. The PCR was carried out using a 50 µl (total volume) mixture containing 5 µl of 10× Taq reaction buffer, 10 µl of 5× Taq Enhancer, 1.5 mM magnesium diacetate, each dNTP at a concentration of 400 µM, 0.4 µM primer WO1 (5′-AGAGTTTGATC[AC]TGGCTC-3′), 0.4 µM primer WO12 (5′-TACGCATTTCACC[GT]CTACA-3′), 1 U of Eppendorf Master Taq polymerase, and 1 µl of template DNA.

Article Title: Native Bacterial Endophytes Promote Host Growth in a Species-Specific Manner; Phytohormone Manipulations Do Not Result in Common Growth Responses
Article Snippet: Identification of endophytic bacterial isolates by 16S rRNA gene sequencing Total bacterial DNA was isolated from 1-day-old cultures on agar plates. .. Amplification of 16S rDNA was performed in a 10 µl final volume containing 1 µl of total DNA, 10 µM of primer F27 ( 5′-AGAGTTTATCMTGGCTCAG-3′ ) and R1492 ( 5′-GRTACCTTGTTACGACTT-3′ ) , 10 mM of each dNTP, 5 mM MgCl2 and 0.05U of Taq DNA polymerase (Eppendorf, Hamburg, Germany).

Transferring:

Article Title: Expression of Connexin36 in Cone Pedicles and OFF-Cone Bipolar Cells of the Mouse Retina
Article Snippet: Subsequently, the tip of the pipette was broken into a PCR tube containing 20 U of RNase inhibitor (Rnasin; Promega, Madison, WI), and the sample (∼8 μl) was subjected to a brief centrifugation. .. This included 2 and 6 μl retinal and photoreceptor cDNA, respectively, 1× reaction buffer (Promega), 1.25 m m MgCl2 , 0.2 m m of each dNTP (Eppendorf), 0.8 μ m of each primer (MWG Biotech, Ebersberg, Germany), and 1 U of Taq polymerase (Promega).

Size-exclusion Chromatography:

Article Title: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
Article Snippet: Polymerase chain reactions (final volumes 50 μl) containing 1× HiFi Buffer (Eppendorf, Westbury, NY), 0.2 mM each dNTP, 0.25 uM each primer, 50 ng of the template plasmids, and 2 U Triple Master DNA Polymerase (Eppendorf, Westbury, NY) were carried out to obtain the CHD7 novel isoform coding sequences from clones M1, M2 and M3. .. The cycling parameters were as follows: 2 min at 94°C (initial denaturation); 24 cycles of 30 sec at 94°C, 30 sec at 58°C and 4 min at 68°C; and 10 min at 68°C (final extension).

Blocking Assay:

Article Title: Traceability of Plant Diet Contents in Raw Cow Milk Samples
Article Snippet: Each PCR-based DNA amplification was performed in a total volume of 30 μL, in 1 × Taq Advanced buffer (Eppendorf), 2 mM MgCl2 , 200 μM of each dNTP, 1 μM of each Primer and 1 U of Taq DNA Polymerase (Eppendorf). .. All PCR reactions were “hot started”: PCR tubes were placed in the PCR thermocycler machine when the block temperature reached 99 °C and then the normal temperature cycle profile was started as follow: 5 min at 95 °C for the initial denaturation, 45 cycles of amplification (30 s at 95 °C, 40 s at 60 °C, and 30 s at 72 °C), and 5 min at 72 °C for the final extension.

Purification:

Article Title: Screening and Transcriptional Analysis of Polyketide Synthases and Non-ribosomal Peptide Synthetases in Bacterial Strains From Krubera–Voronja Cave
Article Snippet: Amplification was carried out in 50 μL of reaction mixture containing PCR buffer with (NH4 )2 SO4 , 2 mM MgCl2 , 0.2 mM each dNTP, 0.25 μM of each primer, 1.25 U recombinant Taq DNA Polymerase, and 10 ng of bacterial genomic DNA in an Eppendorf Mastercycler EP Gradient (Eppendorf, Hamburg, Germany). .. PCR products of the correct size were purified using either the GeneJET PCR Purification Kit or the GeneJET Gel Extraction Kit (Thermo Fisher Scientific), and cloned into E. coli DH5α using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.

Article Title: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
Article Snippet: Polymerase chain reactions (final volumes 50 μl) containing 1× HiFi Buffer (Eppendorf, Westbury, NY), 0.2 mM each dNTP, 0.25 uM each primer, 50 ng of the template plasmids, and 2 U Triple Master DNA Polymerase (Eppendorf, Westbury, NY) were carried out to obtain the CHD7 novel isoform coding sequences from clones M1, M2 and M3. .. The purified PCR products were digested by XhoI and BamHI and sub-cloned into the pLV-EGFP vector digested with the same enzymes.

Article Title: Smenamides A and B, Chlorinated Peptide/Polyketide Hybrids Containing a Dolapyrrolidinone Unit from the Caribbean Sponge Smenospongia aurea. Evaluation of Their Role as Leads in Antitumor Drug Research
Article Snippet: The reaction mixture (25 µL) contained: 14.65 µL H2 O, 0.5 µL DMSO, 1.5 µL dNTP (10 mM), 2.5 µL Taq buffer advanced (Eppendorf, Hamburg, Germany), 2.5 µL forward primer CYA106F (10 µM), 2.5 µL reverse primer CYA781R(a) or CYA781R(b) (10 µM), 0.35 µL RBC Taq DNA polymerase (5 U/µL, RBC Bioscience, New Taipei City, Taiwan), 0.5 µL DNA. .. The PCR products of the expected size (~670 bp) were purified from the agarose gel using QIAquick gel ex kit (Qiagen, Venlo, The Netherlands), subcloned via T/A cloning into pBluescriptII SK(+) (Stratagene California, La Jolla, CA, USA), and transferred to E. coli XL1 Blue MRF' (Stratagene California, La Jolla, CA, USA) electrocompetent cells.

Article Title: Antimicrobial Activity and Genetic Profile of Enteroccoci Isolated from Hoopoes Uropygial Gland
Article Snippet: The PCR was carried out using a 50 µl (total volume) mixture containing 5 µl of 10× Taq reaction buffer, 10 µl of 5× Taq Enhancer, 1.5 mM magnesium diacetate, each dNTP at a concentration of 400 µM, 0.4 µM primer WO1 (5′-AGAGTTTGATC[AC]TGGCTC-3′), 0.4 µM primer WO12 (5′-TACGCATTTCACC[GT]CTACA-3′), 1 U of Eppendorf Master Taq polymerase, and 1 µl of template DNA. .. The amplification program consisted of an initial denaturing step of 94°C for 4 min, followed by amplification using 30 cycles of 30 s at 94°C, 30 s at 50°C, and 60 s at 72°C and then a final extension at 72°C for 2 min. PCR products were purified with a Perfectprep gel cleanup kit (Eppendorf, Hamburg, Germany).

Sequencing:

Article Title: Integrated Operational Taxonomic Units (IOTUs) in Echolocating Bats: A Bridge between Molecular and Traditional Taxonomy
Article Snippet: To amplify the 658 bp target region of coxI , we used primers VF1d (5′-TTCTCAACCAACCACAAR GAYATYGG-3′) and VR1d (5′-TAGACTTCTGGGTGGCCRAARAAYCA-3′) in a 20-µl PCR containing 1X MasterTaq buffer with 1.5 mM MgCl2 (Eppendorf AG, Hamburg, Germany), 0.2 mM of each dNTP, 1 µM of each primer, 1 U of MasterTaq DNA polymerase (Eppendorf AG, Hamburg, Germany) and 1–10 ng of template DNA. .. Sequences were checked by eye and edited manually with BioEdit sequence alignment editor (version 7.0.5 ), and trimmed to yield the same length for all entries in the final alignment.

Article Title: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
Article Snippet: Paragraph title: Cloning of the CHD7 CRA_e isoform coding sequence into a bicistronic lentiviral expression vector ... Polymerase chain reactions (final volumes 50 μl) containing 1× HiFi Buffer (Eppendorf, Westbury, NY), 0.2 mM each dNTP, 0.25 uM each primer, 50 ng of the template plasmids, and 2 U Triple Master DNA Polymerase (Eppendorf, Westbury, NY) were carried out to obtain the CHD7 novel isoform coding sequences from clones M1, M2 and M3.

Article Title: Expression of Connexin36 in Cone Pedicles and OFF-Cone Bipolar Cells of the Mouse Retina
Article Snippet: This included 2 and 6 μl retinal and photoreceptor cDNA, respectively, 1× reaction buffer (Promega), 1.25 m m MgCl2 , 0.2 m m of each dNTP (Eppendorf), 0.8 μ m of each primer (MWG Biotech, Ebersberg, Germany), and 1 U of Taq polymerase (Promega). .. The specific primer set for the detection of rhodopsin included the sense primer (5′-CATTGAGCGCTAC GTGGTGGTC-3′) and the antisense primer (5′-ATGAAGATGGGGCCGAAGTTGG-3′), both of which were designed according to the mouse rhodopsin coding sequence (GenBank accession number ; the predicted size of the amplicon was 766 bp).

Article Title: Smenamides A and B, Chlorinated Peptide/Polyketide Hybrids Containing a Dolapyrrolidinone Unit from the Caribbean Sponge Smenospongia aurea. Evaluation of Their Role as Leads in Antitumor Drug Research
Article Snippet: The reaction mixture (25 µL) contained: 14.65 µL H2 O, 0.5 µL DMSO, 1.5 µL dNTP (10 mM), 2.5 µL Taq buffer advanced (Eppendorf, Hamburg, Germany), 2.5 µL forward primer CYA106F (10 µM), 2.5 µL reverse primer CYA781R(a) or CYA781R(b) (10 µM), 0.35 µL RBC Taq DNA polymerase (5 U/µL, RBC Bioscience, New Taipei City, Taiwan), 0.5 µL DNA. .. After blue white screening, plasmid preps were sent to GATC Biotech AG (Köln, Germany) for single read sequencing using the T7 primer.

Article Title: Antimicrobial Activity and Genetic Profile of Enteroccoci Isolated from Hoopoes Uropygial Gland
Article Snippet: The PCR was carried out using a 50 µl (total volume) mixture containing 5 µl of 10× Taq reaction buffer, 10 µl of 5× Taq Enhancer, 1.5 mM magnesium diacetate, each dNTP at a concentration of 400 µM, 0.4 µM primer WO1 (5′-AGAGTTTGATC[AC]TGGCTC-3′), 0.4 µM primer WO12 (5′-TACGCATTTCACC[GT]CTACA-3′), 1 U of Eppendorf Master Taq polymerase, and 1 µl of template DNA. .. The sequence of the 16S rRNA was determined by using CEQ 2000 dye terminator cycle sequencing with a Quick Start kit (Beckman Coulter, California, USA) according to the manufacturer's instructions.

Article Title: Desert Springs: Deep Phylogeographic Structure in an Ancient Endemic Crustacean (Phreatomerus latipes)
Article Snippet: Polymerase Chain Reaction (PCR) amplification of all sequences involved an initial cycle of denaturation at 95°C for 2 min, and 35 subsequent cycles of 94°C for 30 seconds (s), 50°C for 30 s and 72°C for 1 min. PCR was carried out in 25 µl reactions containing 10×Eppendorf Hotmaster® Taq Buffer (Eppendorf, Westbury, NY, USA) containing 2.5 mM Mg2+ , 2.5 mM of each dNTP, 5.0 µM of each primer, 0.1 units of Eppendorf Hotmaster® Taq Polymerase and ∼1 ng of DNA. .. These PCR products were sequenced using the ABI PRISM Big Dye Terminator Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and the ABI PRISM 3700 DNA analyzer.

Article Title: Native Bacterial Endophytes Promote Host Growth in a Species-Specific Manner; Phytohormone Manipulations Do Not Result in Common Growth Responses
Article Snippet: Paragraph title: Identification of endophytic bacterial isolates by 16S rRNA gene sequencing ... Amplification of 16S rDNA was performed in a 10 µl final volume containing 1 µl of total DNA, 10 µM of primer F27 ( 5′-AGAGTTTATCMTGGCTCAG-3′ ) and R1492 ( 5′-GRTACCTTGTTACGACTT-3′ ) , 10 mM of each dNTP, 5 mM MgCl2 and 0.05U of Taq DNA polymerase (Eppendorf, Hamburg, Germany).

Concentration Assay:

Article Title: Antimicrobial Activity and Genetic Profile of Enteroccoci Isolated from Hoopoes Uropygial Gland
Article Snippet: .. The PCR was carried out using a 50 µl (total volume) mixture containing 5 µl of 10× Taq reaction buffer, 10 µl of 5× Taq Enhancer, 1.5 mM magnesium diacetate, each dNTP at a concentration of 400 µM, 0.4 µM primer WO1 (5′-AGAGTTTGATC[AC]TGGCTC-3′), 0.4 µM primer WO12 (5′-TACGCATTTCACC[GT]CTACA-3′), 1 U of Eppendorf Master Taq polymerase, and 1 µl of template DNA. .. The amplification program consisted of an initial denaturing step of 94°C for 4 min, followed by amplification using 30 cycles of 30 s at 94°C, 30 s at 50°C, and 60 s at 72°C and then a final extension at 72°C for 2 min. PCR products were purified with a Perfectprep gel cleanup kit (Eppendorf, Hamburg, Germany).

Incubation:

Article Title: The role of Rhodotorula mucilaginosa in selected biological process of wild fish
Article Snippet: Cultures were incubated on the YEPD medium (1.0% yeast extract, 2.0%—peptone, 2.0%—glucose; Scharlau) at 22 °C for 24 h. Genetic differentiation analysis was carried out with the random amplified polymorphic DNA method (RAPD-PCR). .. The RAPD-PCR reaction was conducted in 25 μL of the reaction mixture containing the following: 500 mM KCl, 100 mM Tris–HCl (pH 8.3 at 25 °C), 1.25 mM MgCl2 , 0.3 mM dNTP, 20 pmol/μL of each starter, 1 U Taq DNA polymerase (Eppendorf), and 20 ng DNA template.

Article Title: Expression of Connexin36 in Cone Pedicles and OFF-Cone Bipolar Cells of the Mouse Retina
Article Snippet: After primer annealing for 10 min at 72°C, the samples were briefly chilled on ice and incubated for 2 min at 42°C, before 0.3 U/μl avian myeloblastosis virus reverse transcriptase (Promega) were added. cDNA synthesis was performed for 1 hr at 42°C and stopped by incubating the samples for 5 min at 95°C. cDNA was stored at –20°C. .. This included 2 and 6 μl retinal and photoreceptor cDNA, respectively, 1× reaction buffer (Promega), 1.25 m m MgCl2 , 0.2 m m of each dNTP (Eppendorf), 0.8 μ m of each primer (MWG Biotech, Ebersberg, Germany), and 1 U of Taq polymerase (Promega).

DNA Sequencing:

Article Title: Integrated Operational Taxonomic Units (IOTUs) in Echolocating Bats: A Bridge between Molecular and Traditional Taxonomy
Article Snippet: Paragraph title: DNA Extraction, PCR Conditions, DNA Sequencing and Alignment ... To amplify the 658 bp target region of coxI , we used primers VF1d (5′-TTCTCAACCAACCACAAR GAYATYGG-3′) and VR1d (5′-TAGACTTCTGGGTGGCCRAARAAYCA-3′) in a 20-µl PCR containing 1X MasterTaq buffer with 1.5 mM MgCl2 (Eppendorf AG, Hamburg, Germany), 0.2 mM of each dNTP, 1 µM of each primer, 1 U of MasterTaq DNA polymerase (Eppendorf AG, Hamburg, Germany) and 1–10 ng of template DNA.

Expressing:

Article Title: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
Article Snippet: Paragraph title: Cloning of the CHD7 CRA_e isoform coding sequence into a bicistronic lentiviral expression vector ... Polymerase chain reactions (final volumes 50 μl) containing 1× HiFi Buffer (Eppendorf, Westbury, NY), 0.2 mM each dNTP, 0.25 uM each primer, 50 ng of the template plasmids, and 2 U Triple Master DNA Polymerase (Eppendorf, Westbury, NY) were carried out to obtain the CHD7 novel isoform coding sequences from clones M1, M2 and M3.

Modification:

Article Title: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
Article Snippet: This pLV-EGFP bicistronic vector was kindly provided by Prof. Inder Verma (The Salk Institute, San Diego, California) and further modified in our lab by Dr. Juan Carlos Bustos Valenzuela to add the 5'-XbaI-EcoRV-MluI-NheI-PstI-XhoI-BamHI-3' multiple cloning site. .. Polymerase chain reactions (final volumes 50 μl) containing 1× HiFi Buffer (Eppendorf, Westbury, NY), 0.2 mM each dNTP, 0.25 uM each primer, 50 ng of the template plasmids, and 2 U Triple Master DNA Polymerase (Eppendorf, Westbury, NY) were carried out to obtain the CHD7 novel isoform coding sequences from clones M1, M2 and M3.

Gel Extraction:

Article Title: Screening and Transcriptional Analysis of Polyketide Synthases and Non-ribosomal Peptide Synthetases in Bacterial Strains From Krubera–Voronja Cave
Article Snippet: Amplification was carried out in 50 μL of reaction mixture containing PCR buffer with (NH4 )2 SO4 , 2 mM MgCl2 , 0.2 mM each dNTP, 0.25 μM of each primer, 1.25 U recombinant Taq DNA Polymerase, and 10 ng of bacterial genomic DNA in an Eppendorf Mastercycler EP Gradient (Eppendorf, Hamburg, Germany). .. PCR products of the correct size were purified using either the GeneJET PCR Purification Kit or the GeneJET Gel Extraction Kit (Thermo Fisher Scientific), and cloned into E. coli DH5α using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.

Recombinant:

Article Title: Screening and Transcriptional Analysis of Polyketide Synthases and Non-ribosomal Peptide Synthetases in Bacterial Strains From Krubera–Voronja Cave
Article Snippet: .. Amplification was carried out in 50 μL of reaction mixture containing PCR buffer with (NH4 )2 SO4 , 2 mM MgCl2 , 0.2 mM each dNTP, 0.25 μM of each primer, 1.25 U recombinant Taq DNA Polymerase, and 10 ng of bacterial genomic DNA in an Eppendorf Mastercycler EP Gradient (Eppendorf, Hamburg, Germany). .. Amplification conditions for each primer pair were selected according to the references listed in .

Plasmid Preparation:

Article Title: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
Article Snippet: Paragraph title: Cloning of the CHD7 CRA_e isoform coding sequence into a bicistronic lentiviral expression vector ... Polymerase chain reactions (final volumes 50 μl) containing 1× HiFi Buffer (Eppendorf, Westbury, NY), 0.2 mM each dNTP, 0.25 uM each primer, 50 ng of the template plasmids, and 2 U Triple Master DNA Polymerase (Eppendorf, Westbury, NY) were carried out to obtain the CHD7 novel isoform coding sequences from clones M1, M2 and M3.

Article Title: Smenamides A and B, Chlorinated Peptide/Polyketide Hybrids Containing a Dolapyrrolidinone Unit from the Caribbean Sponge Smenospongia aurea. Evaluation of Their Role as Leads in Antitumor Drug Research
Article Snippet: The reaction mixture (25 µL) contained: 14.65 µL H2 O, 0.5 µL DMSO, 1.5 µL dNTP (10 mM), 2.5 µL Taq buffer advanced (Eppendorf, Hamburg, Germany), 2.5 µL forward primer CYA106F (10 µM), 2.5 µL reverse primer CYA781R(a) or CYA781R(b) (10 µM), 0.35 µL RBC Taq DNA polymerase (5 U/µL, RBC Bioscience, New Taipei City, Taiwan), 0.5 µL DNA. .. After blue white screening, plasmid preps were sent to GATC Biotech AG (Köln, Germany) for single read sequencing using the T7 primer.

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