dntp set  (Thermo Fisher)


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    Name:
    dNTP Set
    Description:
    The 100 mM dNTP Set consists of four deoxynucleotides dATP dCTP dGTP dTTP each at a concentration of 100 mM The deoxynucleotides are suitable for use in polymerase chain reaction PCR sequencing fill in nick translation cDNA synthesis and TdT tailing reactions The nucleotides are supplied as ready to use solutions
    Catalog Number:
    10297018
    Price:
    None
    Applications:
    ChIP-on-Chip|PCR|PCR & Real-Time PCR|PCR Buffers, dNTPs, MgCl2 & General Reagents|RNAi, Epigenetics & Non-Coding RNA Research|Chromatin Biology
    Category:
    Oligos Primers Probes Nucleotides
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    Structured Review

    Thermo Fisher dntp set
    The 100 mM dNTP Set consists of four deoxynucleotides dATP dCTP dGTP dTTP each at a concentration of 100 mM The deoxynucleotides are suitable for use in polymerase chain reaction PCR sequencing fill in nick translation cDNA synthesis and TdT tailing reactions The nucleotides are supplied as ready to use solutions
    https://www.bioz.com/result/dntp set/product/Thermo Fisher
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    dntp set - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Reciprocal intronic and exonic histone modification regions in humans
    Article Snippet: .. For each qPCR sample, 0.5 μl of 50 μl eluted ChIP DNA was amplified in 25 μl volume reactions containing 250 μM dNTPs, 1 × (NH4 )2 SO4 buffer (Fermentas), 0.5 μM primer, 1.5 mM MgCl2 , 1.25 units Dynazyme II (Finnzymes), and Sybr Green I fluorescent dye (Sigma). .. Fluorescence was measured on a BioRad CFX-96 machine using standard cycling conditions (3 min at 95°C, 40 cycles of 15 s at 95°C, 30 s at 55°C, and 15 s at 72°C).

    Amplification:

    Article Title: Reciprocal intronic and exonic histone modification regions in humans
    Article Snippet: .. For each qPCR sample, 0.5 μl of 50 μl eluted ChIP DNA was amplified in 25 μl volume reactions containing 250 μM dNTPs, 1 × (NH4 )2 SO4 buffer (Fermentas), 0.5 μM primer, 1.5 mM MgCl2 , 1.25 units Dynazyme II (Finnzymes), and Sybr Green I fluorescent dye (Sigma). .. Fluorescence was measured on a BioRad CFX-96 machine using standard cycling conditions (3 min at 95°C, 40 cycles of 15 s at 95°C, 30 s at 55°C, and 15 s at 72°C).

    Article Title: A multicenter comparison of quantification methods for antisense oligonucleotide-induced DMD exon 51 skipping in Duchenne muscular dystrophy cell cultures
    Article Snippet: .. For the amplification 25 μl PCR reactions containing 1 x Supertaq PCR buffer (# TPRB, Sphaero Q), 0.2 mM of each dNTP (#10297018, Thermo Fisher Scientific), 0.4 μM forward primer (h47F1, Eurogentec, see ), 0.4 μM reverse primer (h54R, Eurogentec, see ), 0.025 U/μl Taq DNA polymerase (#11146165001, Roche) and 3 μl undiluted cDNA were prepared. .. Samples were run in a PCR machine for 5 min at 94°C, 20 cycles of 40 sec at 94°C, 40 sec at 60°C and 80 sec at 72°C, then 7 min at 72°C and cooled down to room temperature.

    Polymerase Chain Reaction:

    Article Title: Physiological and molecular alterations promoted by Schizotetranychus oryzae mite infestation in rice leaves
    Article Snippet: .. RT-qPCRs were carried out in 20 μl final volume composed of 10 μl of each reverse transcription sample diluted 100 times, 2 μl of 10X PCR buffer, 1.2 μl of 50 mM MgCl2, 0.1 μl of 5 mM dNTPs, 0.4 μl of 10 μM primer pairs, 4.25 μl of water, 2.0 μl of SYBR green (1:10,000, Molecular Probe), and 0.05 μl of Platinum Taq DNA polymerase (5 U/μl, Invitrogen, Carlsbad, CA, USA). .. Gene expression was evaluated using a modified 2−ΔCT method , which takes into account the PCR efficiencies of each primer pair (Relative expression TESTED GENE/CONTROL GENE = (PCReff CG)Ct CG /(PCReff TG)Ct TG ).

    Article Title: A multicenter comparison of quantification methods for antisense oligonucleotide-induced DMD exon 51 skipping in Duchenne muscular dystrophy cell cultures
    Article Snippet: .. For the amplification 25 μl PCR reactions containing 1 x Supertaq PCR buffer (# TPRB, Sphaero Q), 0.2 mM of each dNTP (#10297018, Thermo Fisher Scientific), 0.4 μM forward primer (h47F1, Eurogentec, see ), 0.4 μM reverse primer (h54R, Eurogentec, see ), 0.025 U/μl Taq DNA polymerase (#11146165001, Roche) and 3 μl undiluted cDNA were prepared. .. Samples were run in a PCR machine for 5 min at 94°C, 20 cycles of 40 sec at 94°C, 40 sec at 60°C and 80 sec at 72°C, then 7 min at 72°C and cooled down to room temperature.

    Article Title: A multicenter comparison of quantification methods for antisense oligonucleotide-induced DMD exon 51 skipping in Duchenne muscular dystrophy cell cultures
    Article Snippet: .. For the nested PCR, 1.5 μl of the first PCR product was mixed with 48.5 μl PCR master mix with final concentrations of 1 x Supertaq PCR buffer (# TPRB, Sphaero Q), 0.2 mM dNTPs (#10297018, Thermo Fisher Scientific), 0.4 μM forward primer (h47F2, Eurogentec, see ), 0.4 μM reverse primer (h53R2, Eurogentec, see ) and 0.025 U/μl Taq DNA polymerase (#11146165001, Roche). .. The 50 μl PCR reactions were run for 5 min at 94°C, 32 cycles of 40 sec at 94°C, 40 sec at 60°C and 60 sec at 72°C, then 7 min at 72°C and cooled down to room temperature.

    Article Title: Expression profiles of the immune genes CD4, CD8 β, IFN γ, IL-4, IL-6 and IL-10 in mitogen-stimulated koala lymphocytes (Phascolarctos cinereus) by qRT-PCR
    Article Snippet: .. PCR amplifications were carried out in an MJ MiniTM thermal cycler (Bio-Rad Laboratories Inc., CA, USA) in 25 μ l reactions containing 0.4 μ M each primer (Sigma-Aldrich, Castle Hill, NSW, Australia), 1 × High Fidelity PCR Buffer, 1.5 mM MgSO4 , 0.2 mM dNTPs, and 0.75 units of Platinum® Taq DNA Polymerase High Fidelity (Invitrogen, Mulgrave, VIC, Australia). cDNA (10–30 ng) was used for all PCR reactions with the exception of IL-6, where genomic DNA was used as primers designed were positioned within introns due to a scarcity of suitable conserved primer sites within the exons. .. Cycle conditions for touchdown PCR were: an initial activation step of 95°C for 5 min, then cycles of 94°C for 30 s (denaturation), highest annealing temperature for 30 s (annealing), 72°C for 30 s (extension) with the annealing temperature reducing −1°C per cycle until lowest annealing temperature was reached, followed by 20–25 cycles at the lowest annealing temperature, and a final extension at 72°C for 10 min. Amplicons were visualised by electrophoresis on a 1% agarose gel, and those of expected length were excised, purified using UltraClean GelSpin DNA Extraction Kit (Mo Bio, Calsbad, CA, USA) and sequenced commercially in forward and reverse strands (Macrogen, South Korea).

    Incubation:

    Article Title: LncRNAs expression signatures of human brain arteriovenous malformation revealed by microarray
    Article Snippet: .. Samples of cDNA were generated by reverse transcription with 5 μg total RNA, 50 ng random hexamerprimers, 10 nM dNTPs, incubated at 65°C for 5 minutes, and placed on ice for at least 1 minutes, with the addition of 40 URNase OUT, 200U Superscript III RT, 10 mM dithiothreitole, and 5 mM MgCl2 (Invitrogen), in a 20 μL reaction volume. ..

    SYBR Green Assay:

    Article Title: Reciprocal intronic and exonic histone modification regions in humans
    Article Snippet: .. For each qPCR sample, 0.5 μl of 50 μl eluted ChIP DNA was amplified in 25 μl volume reactions containing 250 μM dNTPs, 1 × (NH4 )2 SO4 buffer (Fermentas), 0.5 μM primer, 1.5 mM MgCl2 , 1.25 units Dynazyme II (Finnzymes), and Sybr Green I fluorescent dye (Sigma). .. Fluorescence was measured on a BioRad CFX-96 machine using standard cycling conditions (3 min at 95°C, 40 cycles of 15 s at 95°C, 30 s at 55°C, and 15 s at 72°C).

    Article Title: Physiological and molecular alterations promoted by Schizotetranychus oryzae mite infestation in rice leaves
    Article Snippet: .. RT-qPCRs were carried out in 20 μl final volume composed of 10 μl of each reverse transcription sample diluted 100 times, 2 μl of 10X PCR buffer, 1.2 μl of 50 mM MgCl2, 0.1 μl of 5 mM dNTPs, 0.4 μl of 10 μM primer pairs, 4.25 μl of water, 2.0 μl of SYBR green (1:10,000, Molecular Probe), and 0.05 μl of Platinum Taq DNA polymerase (5 U/μl, Invitrogen, Carlsbad, CA, USA). .. Gene expression was evaluated using a modified 2−ΔCT method , which takes into account the PCR efficiencies of each primer pair (Relative expression TESTED GENE/CONTROL GENE = (PCReff CG)Ct CG /(PCReff TG)Ct TG ).

    Nested PCR:

    Article Title: A multicenter comparison of quantification methods for antisense oligonucleotide-induced DMD exon 51 skipping in Duchenne muscular dystrophy cell cultures
    Article Snippet: .. For the nested PCR, 1.5 μl of the first PCR product was mixed with 48.5 μl PCR master mix with final concentrations of 1 x Supertaq PCR buffer (# TPRB, Sphaero Q), 0.2 mM dNTPs (#10297018, Thermo Fisher Scientific), 0.4 μM forward primer (h47F2, Eurogentec, see ), 0.4 μM reverse primer (h53R2, Eurogentec, see ) and 0.025 U/μl Taq DNA polymerase (#11146165001, Roche). .. The 50 μl PCR reactions were run for 5 min at 94°C, 32 cycles of 40 sec at 94°C, 40 sec at 60°C and 60 sec at 72°C, then 7 min at 72°C and cooled down to room temperature.

    Generated:

    Article Title: LncRNAs expression signatures of human brain arteriovenous malformation revealed by microarray
    Article Snippet: .. Samples of cDNA were generated by reverse transcription with 5 μg total RNA, 50 ng random hexamerprimers, 10 nM dNTPs, incubated at 65°C for 5 minutes, and placed on ice for at least 1 minutes, with the addition of 40 URNase OUT, 200U Superscript III RT, 10 mM dithiothreitole, and 5 mM MgCl2 (Invitrogen), in a 20 μL reaction volume. ..

    CTG Assay:

    Article Title: MGB probe assay for rapid detection of mtDNA11778 mutation in the Chinese LHON patients by real-time PCR
    Article Snippet: .. Primers of dNTPs (P1: 5′-CAT CCT CAT TAC TAT TCT GCC TAG CA-3′; P2: 5′-GGA GTA GAG TTT GAA GTC CTT GAG A-3′) and probe sequences (MT-1: 5′-FAM-CAC TCA CAG TCA CAT CA-MGB-3′; MT-2: 5′-VIC-AGG ATT ATG ATG CGA CTG T-MGB-3′) were provided by Invitrogen Corporation (Carlsbad, California, USA). ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: MGB probe assay for rapid detection of mtDNA11778 mutation in the Chinese LHON patients by real-time PCR
    Article Snippet: .. Primers of dNTPs (P1: 5′-CAT CCT CAT TAC TAT TCT GCC TAG CA-3′; P2: 5′-GGA GTA GAG TTT GAA GTC CTT GAG A-3′) and probe sequences (MT-1: 5′-FAM-CAC TCA CAG TCA CAT CA-MGB-3′; MT-2: 5′-VIC-AGG ATT ATG ATG CGA CTG T-MGB-3′) were provided by Invitrogen Corporation (Carlsbad, California, USA). ..

    Chromatin Immunoprecipitation:

    Article Title: Reciprocal intronic and exonic histone modification regions in humans
    Article Snippet: .. For each qPCR sample, 0.5 μl of 50 μl eluted ChIP DNA was amplified in 25 μl volume reactions containing 250 μM dNTPs, 1 × (NH4 )2 SO4 buffer (Fermentas), 0.5 μM primer, 1.5 mM MgCl2 , 1.25 units Dynazyme II (Finnzymes), and Sybr Green I fluorescent dye (Sigma). .. Fluorescence was measured on a BioRad CFX-96 machine using standard cycling conditions (3 min at 95°C, 40 cycles of 15 s at 95°C, 30 s at 55°C, and 15 s at 72°C).

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  • 99
    Thermo Fisher dntp set
    Dntp Set, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp set/product/Thermo Fisher
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    dntp set - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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