dntp mix  (Roche)


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    Structured Review

    Roche dntp mix
    Dntp Mix, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp mix/product/Roche
    Average 94 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Effect of endometriosis on the fecal bacteriota composition of mice during the acute phase of lesion formation
    Article Snippet: .. The PCR mixture consisted of 0.5 μL of each primer (Integrated DNA Technologies, Leuven, Belgium) (10 μM), 0.6 μL of dNTP-Mix (10 mM, each), 5 μL 5x KAPA Hifi Puffer including 20 mM MgCl2 , 0.1 μL KAPA Hifi Polymerase (Roche, Mannheim, Germany), 1 μL DNA template and was filled up to a final volume of 25 μL with nuclease free water. .. PCR reactions were performed in a T100 Thermal Cycler (Bio-Rad Laboratories, Munich, Germany) using the following thermal profile: 3 min at 95°C for initial denaturation, 25 cycles of 30 s at 95°C for denaturation, 30 s at 55°C for annealing and 45 s at 72°C for elongation, followed by a final elongation step for 5 min at 72°C.

    Article Title: Epigenetic regulation of leptin affects MMP-13 expression in osteoarthritic chondrocytes: possible molecular target for osteoarthritis therapeutic intervention
    Article Snippet: .. LightCycler‐FastStart DNA master SYBR Green, which contains Taq DNA polymerase, dNTP mix, SYBR Green 1 dye and MgCl2 (Roche), was used as a reaction mix for PCR. .. LightCycler Primer set (Roche, Indianapolis, IN) was used for the primers for porphobilinogen deaminase (PBGD) as a housekeeping gene.

    Article Title: Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution
    Article Snippet: .. The dNTP mix, FastStart 10× buffer with MgCl2 and FastStart HiFi polymerase were included in a FastStart High Fidelity PCR System, dNTP Pack (Roche Applied Science, Penzberg, Germany). .. The PCR conditions were as follows: 95°C for 2 min, 30 cycles of 95°C for 20 s, 56°C for 30 s, 72°C for 5 min and final step at 4°C.

    Article Title: Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting
    Article Snippet: .. The PCR mixture with a final volume of 25 µl consisted of 0·5 µl of each primer (10 µm ), 0·6 µl of dNTP-mix (10 mm each), 5 µl 5× KAPA Hifi Puffer including 20 mm -MgCl2 (Roche), 0·1 µl KAPA Hifi Polymerase (Roche), 1 µl DNA isolate and filled up with nuclease-free water. .. PCR reactions were performed in a T100 Thermal Cycler (Bio-Rad Laboratories) using the following programme: 3 min at 95°C for initial denaturation, twenty-five cycles of 30 s at 95°C for denaturation, 30 s at 55°C for annealing, and 45 s at 72°C for elongation, followed by a final elongation step for 5 min at 72°C.

    Article Title: Effect of endometriosis on the fecal bacteriota composition of mice during the acute phase of lesion formation
    Article Snippet: .. The index PCR reaction included 5 μL of Nextera XT (Illumina) Index Primer 1 and 5 μL of Nextera XT Index Primer 2 with 1.2 μL of dNTP-Mix (10 mM each), 10 μL 5x KAPA Hifi Puffer including 20 mM MgCl2 , 0.2 μL KAPA Hifi Polymerase (Roche), 5 μL amplicon DNA and was filled up to 50 μL with nuclease free water. .. PCR reactions were performed in a T100 Thermal Cycler (Bio-Rad Laboratories) using the program detailed above, albeit with 8 cycles.

    Article Title: Acquired resistance to PI3K/mTOR inhibition is associated with mitochondrial DNA mutation and glycolysis
    Article Snippet: .. The PCR mix comprised 100 ng DNA, 2.5 μl of PCR buffer with MgCl2 , 0.5 μl of DNTP mix, 0.2 μl of Taq Polymerase (Roche), and 2.5 μl of forward and reverse primer (IDT) in a total volume of 25μl. .. The primers were as follows: Nuclear DNA Forward: 5′-CAT TGC TCC TCC TGA GCG CAA-3′, Nuclear DNA Reverse: 5′-GCT GTC ACC TTC ACC GTT CCA-3′, Mitochondrial DNA Forward: 5′-ACA ATA GCT AAG ACC CAA ACT GGG-3′, Mitochondrial DNA Reverse: 5′-CCA TTT CTT GCC ACC TCA TGG GC-3′.

    SYBR Green Assay:

    Article Title: Epigenetic regulation of leptin affects MMP-13 expression in osteoarthritic chondrocytes: possible molecular target for osteoarthritis therapeutic intervention
    Article Snippet: .. LightCycler‐FastStart DNA master SYBR Green, which contains Taq DNA polymerase, dNTP mix, SYBR Green 1 dye and MgCl2 (Roche), was used as a reaction mix for PCR. .. LightCycler Primer set (Roche, Indianapolis, IN) was used for the primers for porphobilinogen deaminase (PBGD) as a housekeeping gene.

    Amplification:

    Article Title: Effect of endometriosis on the fecal bacteriota composition of mice during the acute phase of lesion formation
    Article Snippet: .. The index PCR reaction included 5 μL of Nextera XT (Illumina) Index Primer 1 and 5 μL of Nextera XT Index Primer 2 with 1.2 μL of dNTP-Mix (10 mM each), 10 μL 5x KAPA Hifi Puffer including 20 mM MgCl2 , 0.2 μL KAPA Hifi Polymerase (Roche), 5 μL amplicon DNA and was filled up to 50 μL with nuclease free water. .. PCR reactions were performed in a T100 Thermal Cycler (Bio-Rad Laboratories) using the program detailed above, albeit with 8 cycles.

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  • 92
    Roche dntps mix
    Dntps Mix, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps mix/product/Roche
    Average 92 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    dntps mix - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    94
    Roche dntp mix
    Dntp Mix, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp mix/product/Roche
    Average 94 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    91
    Roche deoxynucleoside triphosphate dntp mix
    Deoxynucleoside Triphosphate Dntp Mix, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate dntp mix/product/Roche
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate dntp mix - by Bioz Stars, 2020-09
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      Buy from Supplier

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