dntp mix promega u1515  (Promega)

 
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    Name:
    dNTP Mix
    Description:
    Premixed solution of dATP dCTP dGTP and dTTP in water
    Catalog Number:
    u1511
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis PCR Endpoint PCR
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    Structured Review

    Promega dntp mix promega u1515
    Premixed solution of dATP dCTP dGTP and dTTP in water
    https://www.bioz.com/result/dntp mix promega u1515/product/Promega
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dntp mix promega u1515 - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Selective killing of B-cell hybridomas targeting proteinase 3, Wegener's autoantigen
    Article Snippet: PCR was performed in 50-μl reaction mixtures applying buffer and dNTP mix from Promega (Mannheim, Germany). .. Products from this PCR were restricted with Sfi I and Not I and cloned in the multiple cloning site of pMS III/G, pMS NAng III/G and pMS Ang II/G by direct insertion using the Sfi I and Not I restriction sites.

    Article Title: Genome-wide development of transposable elements-based markers in foxtail millet and construction of an integrated database
    Article Snippet: The PCR amplification reactions were performed in a 25 µl reaction volume containing 100 ng of genomic DNA, 1× Taq buffer, 2 mM of MgCl2 , 0.2 mM dNTP mix (Promega), 0.5 mM each of the forward and reverse primers and four units of Taq polymerase (Biotools). .. The DNA bands were eluted from the gel using Real Genomics Hi Yield Gel/PCR Fragments Extraction Kit (Real Biotech Corporation) and cloned into pGEM® -T Easy vector (Promega) following the manufacturer's instructions.

    Amplification:

    Article Title: Genetic Analysis of 10 Unrelated Korean Families with p22-phox-deficient Chronic Granulomatous Disease: An Unusually Identical Mutation of the CYBA Gene on Jeju Island, Korea
    Article Snippet: .. The synthesized cDNA was amplified using primers (Forward, 5'-ATG GGG CAG ATC GAG TGG GC-3'and Reverse, 5'-TCA CAC GAC CTC GTC GGT CA-3'; CYBA accession number, NM_000101) in a 50-µL PCR reaction containing 0.2 mM dNTP mix, 0.4 µM primer, and 1.25 units of Taq polymerase (Promega). ..

    Article Title: Selective killing of B-cell hybridomas targeting proteinase 3, Wegener's autoantigen
    Article Snippet: Paragraph title: Amplification of PR3 cDNA and plasmid construction ... PCR was performed in 50-μl reaction mixtures applying buffer and dNTP mix from Promega (Mannheim, Germany).

    Article Title: A simple algorithm for quantifying DNA methylation levels on multiple independent CpG sites in bisulfite genomic sequencing electropherograms
    Article Snippet: Each 25-μl PCR reaction included 0.65 U of Hot Star Taq polymerase, 0.22 mM Promega dNTP mix (Promega, Madison, WI, USA) and 0.8 μM of each primer. .. The sequences amplified were from the mouse Avy allele of agouti ( ) (Genbank AR302985).

    Article Title: Angiopoietin-1 targeted RNA interference suppresses angiogenesis and tumor growth of esophageal cancer
    Article Snippet: Reverse transcription reactions were carried out for 1 h at 42°C with 1 μg of total RNA, 250 ng of oligo(dT), 1 × deoxynucleotide triphosphate mix, RNase inhibitor (Promega), 1 × RT buffer, and 200 units of SuperScript II RT (Invitrogen) in a total volume of 20 μL. .. Amplification of Ang-1 was performed in 50 μL of reaction mixture consisting of sense and antisense primers for Ang-1: 2 μg of cDNA, 1 × deoxynucleotide triphosphate mix, 1 × PCR buffer, 1.5 mmol/L MgCl2 , and 2.5 units of AmpliTaq Gold DNA Polymerase (Perkin-Elmer, Wellesley, MA).

    Article Title: Molecular Identification of Endophytic Fungi from Banana Leaves (Musa spp.)
    Article Snippet: ITS regions were amplified using ITS1 (5′-TCC GTA GGT GAA CCT GCG G- 3′) and ITS4 (5′-TCC TCC GCT TAT TGA TAT GC- 3′) primers ( ). .. PCR reaction mixture was prepared in 25 μl reaction containing 4 μL 1X PCR buffer, 4 μl 3.5 mM MgCl2 , 0.5 μl of 0.16 mM of dNTP mix (Promega, Seattle, WA, USA), 0.15 μl of 1.75 unit of GoTaq® DNA polymerase (Promega), 4 μl of 0.0275 μM ITS 1 primer, 4 μl of 0.028 μM ITS 4 primer, 0.3 μl template DNA and 8.05 μl of ddH2 O to make up a total volume of 25 μl.

    Article Title: A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W]A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W] [OA]
    Article Snippet: A cDNA template for PCR was generated with a single RT reaction: 1 μg of RNA was mixed with RNase-free water, 2.5 μL of random hexamers (0.1 μg/μL; Invitrogen), and 1 μL of deoxynucleotide triphosphate mix (10 μM) to a final volume of 12 μL, then incubated at 65°C for 5 min. To this, 4 μL of 5× first-strand buffer, 2 μL of 0.1 M DTT, 1 μL of RNasin Plus RNase inhibitor, and 40 units/μL (Promega) and 1 μL (200 units) of SuperScript II (Invitrogen) were added; the mixture was incubated at 42°C for 60 min. PCR was performed in a 10-μL reaction volume. .. The reaction was initiated by activation of the polymerase at 95°C for 10 min, followed by 45 two-step amplification cycles consisting of 15 s of denaturation at 95°C and a 60-s extension at 60°C.

    Article Title: Global Increase in Circular RNA Levels in Myotonic Dystrophy
    Article Snippet: PCR Assays Design and Validation For the experimental analysis of selected circRNAs, we designed PCR assays that allowed the amplification and parallel analysis of circRNAs and their linear counterparts. .. Briefly, PCR was performed in a 10-μl reaction composed of 0.3 μl of a 10-μM dilution of forward and reverse primers (0.6 μl in total; primers were synthesized by Sigma-Aldrich, Saint Louis, MO, USA), 0.125-μl deoxynucleotide triphosphate mix (concentration of each nucleotide was 10 mM) (Promega), 0.05-μl GoTaq DNA Polymerase (concentration 5 u/μl) (Promega), 2-μl 5× colorless GoTaq reaction buffer (containing 7.5 mM MgCl2 ) (Promega), 6.225-μl deionized water, and 1-μl cDNA template.

    Article Title: The intracellular innate immune sensor NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth
    Article Snippet: One Lachnospiraceae specific reverse primer was also create d: Lachno1261R (5′-TCG CTT CCC TTT GTT TAC GC- 3′), which was used with the 16S rRNA forward gene primer 8F (5′-AGA GTT TGA TCC TGG CTC AG- 3′) for PCR amplification. .. PCR was performed with 1 μl of template DNA (approximately 100 ng), 20 pmol of each primer, 8mM dNTP master mix (Promega-U1511), 1 unit GoTaq DNA polymerase (Promega- M3005), PCR buffer (Promega- M3005) and water in a total of 25 μl per reaction.

    Article Title: Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia
    Article Snippet: DNA extraction was carried out using Wizard™ Genomic DNA Purification Kit (Promega Corporation, USA) following the manufacturer's instructions prior to specific PCR amplification. .. The 25 µ L reaction mixtures included 5 µ L of 5x PCR buffer, 2.0 mM MgCl2 , 0.4 μ M of each primer pair, 0.2 mM of dNTP mix, 1.25 U of Taq DNA polymerase (Promega Corporation, USA), and 5 μ L of DNA template.

    Article Title: Genome-wide development of transposable elements-based markers in foxtail millet and construction of an integrated database
    Article Snippet: .. The PCR amplification reactions were performed in a 25 µl reaction volume containing 100 ng of genomic DNA, 1× Taq buffer, 2 mM of MgCl2 , 0.2 mM dNTP mix (Promega), 0.5 mM each of the forward and reverse primers and four units of Taq polymerase (Biotools). .. The PCR reactions were performed in iCycler thermal cycler (Bio-Rad) and with one cycle of 3 min at 94°C, 34 cycles of 60 s at 94°C, 60 s at 60°C, 1.30 min at 72°C and a final extension of 10 min at 72°C.

    Synthesized:

    Article Title: Genetic Analysis of 10 Unrelated Korean Families with p22-phox-deficient Chronic Granulomatous Disease: An Unusually Identical Mutation of the CYBA Gene on Jeju Island, Korea
    Article Snippet: .. The synthesized cDNA was amplified using primers (Forward, 5'-ATG GGG CAG ATC GAG TGG GC-3'and Reverse, 5'-TCA CAC GAC CTC GTC GGT CA-3'; CYBA accession number, NM_000101) in a 50-µL PCR reaction containing 0.2 mM dNTP mix, 0.4 µM primer, and 1.25 units of Taq polymerase (Promega). ..

    Article Title: Selective killing of B-cell hybridomas targeting proteinase 3, Wegener's autoantigen
    Article Snippet: The C-terminal fragments of the PR3 variants were synthesized using the reverse primer 5′-ctg-tct-aga-ctc-gag-t gc - ggc - cgc -GGG-GCG-GCC-CTT-GGC-CTC-CAC-ACG-3′ (bold letters indicate the Not I restriction site) combined with 5′-gtg-ctg-acg-gcc-gcg-CAA-tgc-ctg-cgg-gac-ata-cc-3′ (PR3H44Q), 5′-gag-aac-aaa-ctg-aac-GCC-gtt-ctc-ctc-atc-cag-3′ (PR3D91A) or 5′-ggc-atc-tgc-ttc-gga-gac-GCA-ggt-ggc-ccc-ctg-3′ (PR3S176A) as inner forward primers. .. PCR was performed in 50-μl reaction mixtures applying buffer and dNTP mix from Promega (Mannheim, Germany).

    Article Title: Global Increase in Circular RNA Levels in Myotonic Dystrophy
    Article Snippet: .. Briefly, PCR was performed in a 10-μl reaction composed of 0.3 μl of a 10-μM dilution of forward and reverse primers (0.6 μl in total; primers were synthesized by Sigma-Aldrich, Saint Louis, MO, USA), 0.125-μl deoxynucleotide triphosphate mix (concentration of each nucleotide was 10 mM) (Promega), 0.05-μl GoTaq DNA Polymerase (concentration 5 u/μl) (Promega), 2-μl 5× colorless GoTaq reaction buffer (containing 7.5 mM MgCl2 ) (Promega), 6.225-μl deionized water, and 1-μl cDNA template. ..

    SYBR Green Assay:

    Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
    Article Snippet: Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology). .. The reverse transcription reaction program consisted of three steps: 10 min at 25 °C, 50 min at 37 °C, and 15 min at 70 °C. mRNA expression was measured using a LightCycler 480 real-time PCR system and SYBR Green I Master mix® (Roche Diagnostics) (primers are listed in Additional file : Table S1).

    Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction
    Article Snippet: Complementary DNA (cDNA) was prepared from 1 mg of RNA using deoxynucleotide triphosphate mix, random primers, RNase Inhibitor, reaction buffer and reverse transcriptase as recommended by the manufacturer (Promega, Madison, WI). .. RNA expression was determined using an Applied Biosystems StepOnePlus™ Real-Time PCR System with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA).

    Incubation:

    Article Title: Molecular Identification of Endophytic Fungi from Banana Leaves (Musa spp.)
    Article Snippet: For DNA extraction, the fungal isolates were cultured on the surface of dialysis membrane on PDA, incubated for 5–7 days at 25±1°C or until there was visible mycelia growth. .. PCR reaction mixture was prepared in 25 μl reaction containing 4 μL 1X PCR buffer, 4 μl 3.5 mM MgCl2 , 0.5 μl of 0.16 mM of dNTP mix (Promega, Seattle, WA, USA), 0.15 μl of 1.75 unit of GoTaq® DNA polymerase (Promega), 4 μl of 0.0275 μM ITS 1 primer, 4 μl of 0.028 μM ITS 4 primer, 0.3 μl template DNA and 8.05 μl of ddH2 O to make up a total volume of 25 μl.

    Article Title: A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W]A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W] [OA]
    Article Snippet: .. A cDNA template for PCR was generated with a single RT reaction: 1 μg of RNA was mixed with RNase-free water, 2.5 μL of random hexamers (0.1 μg/μL; Invitrogen), and 1 μL of deoxynucleotide triphosphate mix (10 μM) to a final volume of 12 μL, then incubated at 65°C for 5 min. To this, 4 μL of 5× first-strand buffer, 2 μL of 0.1 M DTT, 1 μL of RNasin Plus RNase inhibitor, and 40 units/μL (Promega) and 1 μL (200 units) of SuperScript II (Invitrogen) were added; the mixture was incubated at 42°C for 60 min. PCR was performed in a 10-μL reaction volume. .. PCR plates (384 wells) were set up with a Freedom Evo liquid-handling robot (Tecan) and then run on a 7900HT sequence detection system (Applied Biosystems).

    Expressing:

    Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
    Article Snippet: Paragraph title: Gene expression ... Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology).

    Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction
    Article Snippet: Complementary DNA (cDNA) was prepared from 1 mg of RNA using deoxynucleotide triphosphate mix, random primers, RNase Inhibitor, reaction buffer and reverse transcriptase as recommended by the manufacturer (Promega, Madison, WI). .. Fold change in gene expression was determined using the ddCt method ( ).

    Countercurrent Chromatography:

    Article Title: The intracellular innate immune sensor NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth
    Article Snippet: One Lachnospiraceae specific reverse primer was also create d: Lachno1261R (5′-TCG CTT CCC TTT GTT TAC GC- 3′), which was used with the 16S rRNA forward gene primer 8F (5′-AGA GTT TGA TCC TGG CTC AG- 3′) for PCR amplification. .. PCR was performed with 1 μl of template DNA (approximately 100 ng), 20 pmol of each primer, 8mM dNTP master mix (Promega-U1511), 1 unit GoTaq DNA polymerase (Promega- M3005), PCR buffer (Promega- M3005) and water in a total of 25 μl per reaction.

    Concentration Assay:

    Article Title: Selective killing of B-cell hybridomas targeting proteinase 3, Wegener's autoantigen
    Article Snippet: PCR was performed in 50-μl reaction mixtures applying buffer and dNTP mix from Promega (Mannheim, Germany). .. Dimethyl sulphoxide was added to a final concentration of 2%.

    Article Title: Global Increase in Circular RNA Levels in Myotonic Dystrophy
    Article Snippet: .. Briefly, PCR was performed in a 10-μl reaction composed of 0.3 μl of a 10-μM dilution of forward and reverse primers (0.6 μl in total; primers were synthesized by Sigma-Aldrich, Saint Louis, MO, USA), 0.125-μl deoxynucleotide triphosphate mix (concentration of each nucleotide was 10 mM) (Promega), 0.05-μl GoTaq DNA Polymerase (concentration 5 u/μl) (Promega), 2-μl 5× colorless GoTaq reaction buffer (containing 7.5 mM MgCl2 ) (Promega), 6.225-μl deionized water, and 1-μl cDNA template. ..

    Serial Dilution:

    Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
    Article Snippet: Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology). .. Standard curves were generated for each run by serial dilution of control cDNA.

    Cell Culture:

    Article Title: Molecular Identification of Endophytic Fungi from Banana Leaves (Musa spp.)
    Article Snippet: For DNA extraction, the fungal isolates were cultured on the surface of dialysis membrane on PDA, incubated for 5–7 days at 25±1°C or until there was visible mycelia growth. .. PCR reaction mixture was prepared in 25 μl reaction containing 4 μL 1X PCR buffer, 4 μl 3.5 mM MgCl2 , 0.5 μl of 0.16 mM of dNTP mix (Promega, Seattle, WA, USA), 0.15 μl of 1.75 unit of GoTaq® DNA polymerase (Promega), 4 μl of 0.0275 μM ITS 1 primer, 4 μl of 0.028 μM ITS 4 primer, 0.3 μl template DNA and 8.05 μl of ddH2 O to make up a total volume of 25 μl.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Genetic Analysis of 10 Unrelated Korean Families with p22-phox-deficient Chronic Granulomatous Disease: An Unusually Identical Mutation of the CYBA Gene on Jeju Island, Korea
    Article Snippet: The synthesized cDNA was amplified using primers (Forward, 5'-ATG GGG CAG ATC GAG TGG GC-3'and Reverse, 5'-TCA CAC GAC CTC GTC GGT CA-3'; CYBA accession number, NM_000101) in a 50-µL PCR reaction containing 0.2 mM dNTP mix, 0.4 µM primer, and 1.25 units of Taq polymerase (Promega). .. The RT-PCR products were separated in a 2% agarose gel and stained with ethidium bromide for visualization.

    Article Title: Angiopoietin-1 targeted RNA interference suppresses angiogenesis and tumor growth of esophageal cancer
    Article Snippet: Paragraph title: RT-PCR for angiopoietin-1 ... Reverse transcription reactions were carried out for 1 h at 42°C with 1 μg of total RNA, 250 ng of oligo(dT), 1 × deoxynucleotide triphosphate mix, RNase inhibitor (Promega), 1 × RT buffer, and 200 units of SuperScript II RT (Invitrogen) in a total volume of 20 μL.

    Generated:

    Article Title: Selective killing of B-cell hybridomas targeting proteinase 3, Wegener's autoantigen
    Article Snippet: In a first step, the N-terminal and C-terminal portions of PR3 variants were generated separately using the outer primer 5′-act-ggt-gac-gc g-gcc-cag-ccg-gcc - ATC-GTG-GGC-GGG-CAC-GAG-3′ (underlined letters indicate the Sfi I restriction site) forward combined with 5′-gg-tat-gtc-ccg-cag-gca-TTG-cgc-ggc-cgt-cag-cac-3′ (PR3H44Q), 5′-ctg-gat-gag-gag-aac-GGC-gtt-cag-ttt-gtt-ctc-3′ (PR3D91A) or 5′-cag-ggg-gcc-acc-TGC-gtc-tcc-gaa-gca-gat-gcc-3′ (PR3S176A) as inner reverse primers. .. PCR was performed in 50-μl reaction mixtures applying buffer and dNTP mix from Promega (Mannheim, Germany).

    Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
    Article Snippet: Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology). .. Standard curves were generated for each run by serial dilution of control cDNA.

    Article Title: A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W]A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W] [OA]
    Article Snippet: .. A cDNA template for PCR was generated with a single RT reaction: 1 μg of RNA was mixed with RNase-free water, 2.5 μL of random hexamers (0.1 μg/μL; Invitrogen), and 1 μL of deoxynucleotide triphosphate mix (10 μM) to a final volume of 12 μL, then incubated at 65°C for 5 min. To this, 4 μL of 5× first-strand buffer, 2 μL of 0.1 M DTT, 1 μL of RNasin Plus RNase inhibitor, and 40 units/μL (Promega) and 1 μL (200 units) of SuperScript II (Invitrogen) were added; the mixture was incubated at 42°C for 60 min. PCR was performed in a 10-μL reaction volume. .. PCR plates (384 wells) were set up with a Freedom Evo liquid-handling robot (Tecan) and then run on a 7900HT sequence detection system (Applied Biosystems).

    Article Title: Global Increase in Circular RNA Levels in Myotonic Dystrophy
    Article Snippet: The only exceptions were assays designed for circCDR1as (circRNA generated from a single-exon transcript) and circMBNL1, which consisted of four primers (two for the circular transcript and two for the linear transcript). .. Briefly, PCR was performed in a 10-μl reaction composed of 0.3 μl of a 10-μM dilution of forward and reverse primers (0.6 μl in total; primers were synthesized by Sigma-Aldrich, Saint Louis, MO, USA), 0.125-μl deoxynucleotide triphosphate mix (concentration of each nucleotide was 10 mM) (Promega), 0.05-μl GoTaq DNA Polymerase (concentration 5 u/μl) (Promega), 2-μl 5× colorless GoTaq reaction buffer (containing 7.5 mM MgCl2 ) (Promega), 6.225-μl deionized water, and 1-μl cDNA template.

    other:

    Article Title: Potential role of oxidative stress-induced apoptosis in mediating chromosomal rearrangements in nasopharyngeal carcinoma
    Article Snippet: Nested IPCR Nested IPCR was performed with 200 ng of DNA template, 1X of HF buffer (containing 1.5 mM of MgCl2 ), 200 µM of dNTP mix, 0.5 µM of each reverse primer and forward primer, and 0.4 U of Phusion High-Fidelity DNA Polymerase.

    Sequencing:

    Article Title: Rapid DNA Amplification Using a Battery-Powered Thin-Film Resistive Thermocycler
    Article Snippet: EDAS 290 digital camera/stand, Kodak, Rochester NY. dNTP mix, PR-U1511, Promega Corp., Madison, WI. .. Oligonucleotide primers, custom sequence, Operon, Huntsville, AL.

    Article Title: A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W]A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W] [OA]
    Article Snippet: A cDNA template for PCR was generated with a single RT reaction: 1 μg of RNA was mixed with RNase-free water, 2.5 μL of random hexamers (0.1 μg/μL; Invitrogen), and 1 μL of deoxynucleotide triphosphate mix (10 μM) to a final volume of 12 μL, then incubated at 65°C for 5 min. To this, 4 μL of 5× first-strand buffer, 2 μL of 0.1 M DTT, 1 μL of RNasin Plus RNase inhibitor, and 40 units/μL (Promega) and 1 μL (200 units) of SuperScript II (Invitrogen) were added; the mixture was incubated at 42°C for 60 min. PCR was performed in a 10-μL reaction volume. .. PCR plates (384 wells) were set up with a Freedom Evo liquid-handling robot (Tecan) and then run on a 7900HT sequence detection system (Applied Biosystems).

    Article Title: Global Increase in Circular RNA Levels in Myotonic Dystrophy
    Article Snippet: Briefly, PCR was performed in a 10-μl reaction composed of 0.3 μl of a 10-μM dilution of forward and reverse primers (0.6 μl in total; primers were synthesized by Sigma-Aldrich, Saint Louis, MO, USA), 0.125-μl deoxynucleotide triphosphate mix (concentration of each nucleotide was 10 mM) (Promega), 0.05-μl GoTaq DNA Polymerase (concentration 5 u/μl) (Promega), 2-μl 5× colorless GoTaq reaction buffer (containing 7.5 mM MgCl2 ) (Promega), 6.225-μl deionized water, and 1-μl cDNA template. .. Additionally, the specificity of each product was confirmed by Sanger sequencing performed on an ABI Prism 3130 genetic analyzer (Applied Biosystems, Carlsbad, CA, USA) according to the manufacturer’s general recommendations.

    DNA Extraction:

    Article Title: Molecular Identification of Endophytic Fungi from Banana Leaves (Musa spp.)
    Article Snippet: Invisorb® Spin Plant Mini Kit (STRATEC Molecular GmbH, Berlin, Germany) was used for DNA extraction according to the manufacturers’ instructions. .. PCR reaction mixture was prepared in 25 μl reaction containing 4 μL 1X PCR buffer, 4 μl 3.5 mM MgCl2 , 0.5 μl of 0.16 mM of dNTP mix (Promega, Seattle, WA, USA), 0.15 μl of 1.75 unit of GoTaq® DNA polymerase (Promega), 4 μl of 0.0275 μM ITS 1 primer, 4 μl of 0.028 μM ITS 4 primer, 0.3 μl template DNA and 8.05 μl of ddH2 O to make up a total volume of 25 μl.

    Article Title: Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia
    Article Snippet: DNA extraction was carried out using Wizard™ Genomic DNA Purification Kit (Promega Corporation, USA) following the manufacturer's instructions prior to specific PCR amplification. .. The 25 µ L reaction mixtures included 5 µ L of 5x PCR buffer, 2.0 mM MgCl2 , 0.4 μ M of each primer pair, 0.2 mM of dNTP mix, 1.25 U of Taq DNA polymerase (Promega Corporation, USA), and 5 μ L of DNA template.

    Mutagenesis:

    Article Title: Selective killing of B-cell hybridomas targeting proteinase 3, Wegener's autoantigen
    Article Snippet: The inner primers inserted a specific point mutation to substitute the amino acids histidine, aspartic acid, or serine, which form the catalytic triad. .. PCR was performed in 50-μl reaction mixtures applying buffer and dNTP mix from Promega (Mannheim, Germany).

    Isolation:

    Article Title: Genetic Analysis of 10 Unrelated Korean Families with p22-phox-deficient Chronic Granulomatous Disease: An Unusually Identical Mutation of the CYBA Gene on Jeju Island, Korea
    Article Snippet: Paragraph title: Isolation of total RNA and RT-RCR ... The synthesized cDNA was amplified using primers (Forward, 5'-ATG GGG CAG ATC GAG TGG GC-3'and Reverse, 5'-TCA CAC GAC CTC GTC GGT CA-3'; CYBA accession number, NM_000101) in a 50-µL PCR reaction containing 0.2 mM dNTP mix, 0.4 µM primer, and 1.25 units of Taq polymerase (Promega).

    Article Title: Angiopoietin-1 targeted RNA interference suppresses angiogenesis and tumor growth of esophageal cancer
    Article Snippet: Total RNA was isolated from Eca109, Eca109/si and Eca109/Ang-1si cells using the Trizol protocol (Invitrogen Biotechnology). .. Reverse transcription reactions were carried out for 1 h at 42°C with 1 μg of total RNA, 250 ng of oligo(dT), 1 × deoxynucleotide triphosphate mix, RNase inhibitor (Promega), 1 × RT buffer, and 200 units of SuperScript II RT (Invitrogen) in a total volume of 20 μL.

    Size-exclusion Chromatography:

    Article Title: The intracellular innate immune sensor NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth
    Article Snippet: PCR was performed with 1 μl of template DNA (approximately 100 ng), 20 pmol of each primer, 8mM dNTP master mix (Promega-U1511), 1 unit GoTaq DNA polymerase (Promega- M3005), PCR buffer (Promega- M3005) and water in a total of 25 μl per reaction. .. PCR reaction was performed under the following cycling conditions: 95°C for 2 min, 30 cycles of 95°C for 30 sec, annealing at 57°C for 45 sec, and extension at 72°C for 90 sec, 72°C for 10 min.

    Article Title: Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia
    Article Snippet: The 25 µ L reaction mixtures included 5 µ L of 5x PCR buffer, 2.0 mM MgCl2 , 0.4 μ M of each primer pair, 0.2 mM of dNTP mix, 1.25 U of Taq DNA polymerase (Promega Corporation, USA), and 5 μ L of DNA template. .. The cycling conditions included initial denaturation at 95°C for 2 min, 35 cycles of each of denaturation at 95°C for 1 min, primer annealing at 55°C for 30 sec, and extension at 72°C for 1 min, further extension at 72°C for 5 min, and indefinite holding period at 4°C.

    Mouse Assay:

    Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction
    Article Snippet: Complementary DNA (cDNA) was prepared from 1 mg of RNA using deoxynucleotide triphosphate mix, random primers, RNase Inhibitor, reaction buffer and reverse transcriptase as recommended by the manufacturer (Promega, Madison, WI). .. The expression of genes of interest was normalized to the expression of GAPDH in humans and 18S ribosomal RNA in mice.

    Polymerase Chain Reaction:

    Article Title: Genetic Analysis of 10 Unrelated Korean Families with p22-phox-deficient Chronic Granulomatous Disease: An Unusually Identical Mutation of the CYBA Gene on Jeju Island, Korea
    Article Snippet: .. The synthesized cDNA was amplified using primers (Forward, 5'-ATG GGG CAG ATC GAG TGG GC-3'and Reverse, 5'-TCA CAC GAC CTC GTC GGT CA-3'; CYBA accession number, NM_000101) in a 50-µL PCR reaction containing 0.2 mM dNTP mix, 0.4 µM primer, and 1.25 units of Taq polymerase (Promega). ..

    Article Title: Selective killing of B-cell hybridomas targeting proteinase 3, Wegener's autoantigen
    Article Snippet: .. PCR was performed in 50-μl reaction mixtures applying buffer and dNTP mix from Promega (Mannheim, Germany). .. Dimethyl sulphoxide was added to a final concentration of 2%.

    Article Title: A simple algorithm for quantifying DNA methylation levels on multiple independent CpG sites in bisulfite genomic sequencing electropherograms
    Article Snippet: .. Each 25-μl PCR reaction included 0.65 U of Hot Star Taq polymerase, 0.22 mM Promega dNTP mix (Promega, Madison, WI, USA) and 0.8 μM of each primer. .. The sequences amplified were from the mouse Avy allele of agouti ( ) (Genbank AR302985).

    Article Title: Rapid DNA Amplification Using a Battery-Powered Thin-Film Resistive Thermocycler
    Article Snippet: Gen AMP PCR System 2400 thermocycler. .. EDAS 290 digital camera/stand, Kodak, Rochester NY. dNTP mix, PR-U1511, Promega Corp., Madison, WI.

    Article Title: Authentication of beef, carabeef, chevon, mutton and pork by a PCR-RFLP assay of mitochondrial cytb gene
    Article Snippet: .. The PCR was set up in a volume of 50 μL containing 5 μL of 10X assay buffer (160 m M (NH4)2SO4, 670 m M Tris-HCl, pH 8.8, 0.1 % tween-20, 25 m M MgCl2 , Bioron-GmbH, Ludwigshafen, Germany), 1 μL (200 μ M each) of dNTP mix (pH 7.5, Promega, Madison, Wisconsin, USA), 1 μL (25 pmol) each of forward and reverse primer (IDT, Iowa, USA), 2.5U Taq DNA polymerase (Bioron-GmbH, Ludwigshafen, Germany), 50 ng of purified DNA and nuclease free water (Merck, Darmstadt, Germany). .. The tubes were flash spun and the PCR was performed in a thermocycler (Gene AMP® PCR System 9700, Applied Biosystems, Foster City, California, USA).

    Article Title: Angiopoietin-1 targeted RNA interference suppresses angiogenesis and tumor growth of esophageal cancer
    Article Snippet: Reverse transcription reactions were carried out for 1 h at 42°C with 1 μg of total RNA, 250 ng of oligo(dT), 1 × deoxynucleotide triphosphate mix, RNase inhibitor (Promega), 1 × RT buffer, and 200 units of SuperScript II RT (Invitrogen) in a total volume of 20 μL. .. Amplification of Ang-1 was performed in 50 μL of reaction mixture consisting of sense and antisense primers for Ang-1: 2 μg of cDNA, 1 × deoxynucleotide triphosphate mix, 1 × PCR buffer, 1.5 mmol/L MgCl2 , and 2.5 units of AmpliTaq Gold DNA Polymerase (Perkin-Elmer, Wellesley, MA).

    Article Title: Molecular Identification of Endophytic Fungi from Banana Leaves (Musa spp.)
    Article Snippet: .. PCR reaction mixture was prepared in 25 μl reaction containing 4 μL 1X PCR buffer, 4 μl 3.5 mM MgCl2 , 0.5 μl of 0.16 mM of dNTP mix (Promega, Seattle, WA, USA), 0.15 μl of 1.75 unit of GoTaq® DNA polymerase (Promega), 4 μl of 0.0275 μM ITS 1 primer, 4 μl of 0.028 μM ITS 4 primer, 0.3 μl template DNA and 8.05 μl of ddH2 O to make up a total volume of 25 μl. ..

    Article Title: A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W]A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W] [OA]
    Article Snippet: .. A cDNA template for PCR was generated with a single RT reaction: 1 μg of RNA was mixed with RNase-free water, 2.5 μL of random hexamers (0.1 μg/μL; Invitrogen), and 1 μL of deoxynucleotide triphosphate mix (10 μM) to a final volume of 12 μL, then incubated at 65°C for 5 min. To this, 4 μL of 5× first-strand buffer, 2 μL of 0.1 M DTT, 1 μL of RNasin Plus RNase inhibitor, and 40 units/μL (Promega) and 1 μL (200 units) of SuperScript II (Invitrogen) were added; the mixture was incubated at 42°C for 60 min. PCR was performed in a 10-μL reaction volume. .. PCR plates (384 wells) were set up with a Freedom Evo liquid-handling robot (Tecan) and then run on a 7900HT sequence detection system (Applied Biosystems).

    Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction
    Article Snippet: Complementary DNA (cDNA) was prepared from 1 mg of RNA using deoxynucleotide triphosphate mix, random primers, RNase Inhibitor, reaction buffer and reverse transcriptase as recommended by the manufacturer (Promega, Madison, WI). .. RNA expression was determined using an Applied Biosystems StepOnePlus™ Real-Time PCR System with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA).

    Article Title: Global Increase in Circular RNA Levels in Myotonic Dystrophy
    Article Snippet: .. Briefly, PCR was performed in a 10-μl reaction composed of 0.3 μl of a 10-μM dilution of forward and reverse primers (0.6 μl in total; primers were synthesized by Sigma-Aldrich, Saint Louis, MO, USA), 0.125-μl deoxynucleotide triphosphate mix (concentration of each nucleotide was 10 mM) (Promega), 0.05-μl GoTaq DNA Polymerase (concentration 5 u/μl) (Promega), 2-μl 5× colorless GoTaq reaction buffer (containing 7.5 mM MgCl2 ) (Promega), 6.225-μl deionized water, and 1-μl cDNA template. ..

    Article Title: The intracellular innate immune sensor NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth
    Article Snippet: .. PCR was performed with 1 μl of template DNA (approximately 100 ng), 20 pmol of each primer, 8mM dNTP master mix (Promega-U1511), 1 unit GoTaq DNA polymerase (Promega- M3005), PCR buffer (Promega- M3005) and water in a total of 25 μl per reaction. .. PCR reaction was performed under the following cycling conditions: 95°C for 2 min, 30 cycles of 95°C for 30 sec, annealing at 57°C for 45 sec, and extension at 72°C for 90 sec, 72°C for 10 min.

    Article Title: Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia
    Article Snippet: .. The 25 µ L reaction mixtures included 5 µ L of 5x PCR buffer, 2.0 mM MgCl2 , 0.4 μ M of each primer pair, 0.2 mM of dNTP mix, 1.25 U of Taq DNA polymerase (Promega Corporation, USA), and 5 μ L of DNA template. .. The cycling conditions included initial denaturation at 95°C for 2 min, 35 cycles of each of denaturation at 95°C for 1 min, primer annealing at 55°C for 30 sec, and extension at 72°C for 1 min, further extension at 72°C for 5 min, and indefinite holding period at 4°C.

    Article Title: Genome-wide development of transposable elements-based markers in foxtail millet and construction of an integrated database
    Article Snippet: .. The PCR amplification reactions were performed in a 25 µl reaction volume containing 100 ng of genomic DNA, 1× Taq buffer, 2 mM of MgCl2 , 0.2 mM dNTP mix (Promega), 0.5 mM each of the forward and reverse primers and four units of Taq polymerase (Biotools). .. The PCR reactions were performed in iCycler thermal cycler (Bio-Rad) and with one cycle of 3 min at 94°C, 34 cycles of 60 s at 94°C, 60 s at 60°C, 1.30 min at 72°C and a final extension of 10 min at 72°C.

    Nested PCR:

    Article Title: A simple algorithm for quantifying DNA methylation levels on multiple independent CpG sites in bisulfite genomic sequencing electropherograms
    Article Snippet: Each 25-μl PCR reaction included 0.65 U of Hot Star Taq polymerase, 0.22 mM Promega dNTP mix (Promega, Madison, WI, USA) and 0.8 μM of each primer. .. Bisulfite-modified genomic DNA was amplified by nested PCR using two sets of primers for the Avy allele similar to that described by Rakyan et al . ( ).

    Activated Clotting Time Assay:

    Article Title: The intracellular innate immune sensor NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth
    Article Snippet: These forward primers were used with the 16S rRNA reverse gene primer 1492R (5′-GGT TAC CTT GTT ACG ACT T- 3′) for PCR amplification. .. PCR was performed with 1 μl of template DNA (approximately 100 ng), 20 pmol of each primer, 8mM dNTP master mix (Promega-U1511), 1 unit GoTaq DNA polymerase (Promega- M3005), PCR buffer (Promega- M3005) and water in a total of 25 μl per reaction.

    Purification:

    Article Title: Authentication of beef, carabeef, chevon, mutton and pork by a PCR-RFLP assay of mitochondrial cytb gene
    Article Snippet: .. The PCR was set up in a volume of 50 μL containing 5 μL of 10X assay buffer (160 m M (NH4)2SO4, 670 m M Tris-HCl, pH 8.8, 0.1 % tween-20, 25 m M MgCl2 , Bioron-GmbH, Ludwigshafen, Germany), 1 μL (200 μ M each) of dNTP mix (pH 7.5, Promega, Madison, Wisconsin, USA), 1 μL (25 pmol) each of forward and reverse primer (IDT, Iowa, USA), 2.5U Taq DNA polymerase (Bioron-GmbH, Ludwigshafen, Germany), 50 ng of purified DNA and nuclease free water (Merck, Darmstadt, Germany). .. The tubes were flash spun and the PCR was performed in a thermocycler (Gene AMP® PCR System 9700, Applied Biosystems, Foster City, California, USA).

    Plasmid Preparation:

    Article Title: Selective killing of B-cell hybridomas targeting proteinase 3, Wegener's autoantigen
    Article Snippet: Paragraph title: Amplification of PR3 cDNA and plasmid construction ... PCR was performed in 50-μl reaction mixtures applying buffer and dNTP mix from Promega (Mannheim, Germany).

    Article Title: Genome-wide development of transposable elements-based markers in foxtail millet and construction of an integrated database
    Article Snippet: The PCR amplification reactions were performed in a 25 µl reaction volume containing 100 ng of genomic DNA, 1× Taq buffer, 2 mM of MgCl2 , 0.2 mM dNTP mix (Promega), 0.5 mM each of the forward and reverse primers and four units of Taq polymerase (Biotools). .. The DNA bands were eluted from the gel using Real Genomics Hi Yield Gel/PCR Fragments Extraction Kit (Real Biotech Corporation) and cloned into pGEM® -T Easy vector (Promega) following the manufacturer's instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
    Article Snippet: Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology). .. The reverse transcription reaction program consisted of three steps: 10 min at 25 °C, 50 min at 37 °C, and 15 min at 70 °C. mRNA expression was measured using a LightCycler 480 real-time PCR system and SYBR Green I Master mix® (Roche Diagnostics) (primers are listed in Additional file : Table S1).

    Article Title: A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W]A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W] [OA]
    Article Snippet: Paragraph title: Real-Time PCR Quantitation and RNA Gel Blotting ... A cDNA template for PCR was generated with a single RT reaction: 1 μg of RNA was mixed with RNase-free water, 2.5 μL of random hexamers (0.1 μg/μL; Invitrogen), and 1 μL of deoxynucleotide triphosphate mix (10 μM) to a final volume of 12 μL, then incubated at 65°C for 5 min. To this, 4 μL of 5× first-strand buffer, 2 μL of 0.1 M DTT, 1 μL of RNasin Plus RNase inhibitor, and 40 units/μL (Promega) and 1 μL (200 units) of SuperScript II (Invitrogen) were added; the mixture was incubated at 42°C for 60 min. PCR was performed in a 10-μL reaction volume.

    Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction
    Article Snippet: Paragraph title: Real-time PCR ... Complementary DNA (cDNA) was prepared from 1 mg of RNA using deoxynucleotide triphosphate mix, random primers, RNase Inhibitor, reaction buffer and reverse transcriptase as recommended by the manufacturer (Promega, Madison, WI).

    RNA Expression:

    Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction
    Article Snippet: Complementary DNA (cDNA) was prepared from 1 mg of RNA using deoxynucleotide triphosphate mix, random primers, RNase Inhibitor, reaction buffer and reverse transcriptase as recommended by the manufacturer (Promega, Madison, WI). .. RNA expression was determined using an Applied Biosystems StepOnePlus™ Real-Time PCR System with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA).

    Agarose Gel Electrophoresis:

    Article Title: Genetic Analysis of 10 Unrelated Korean Families with p22-phox-deficient Chronic Granulomatous Disease: An Unusually Identical Mutation of the CYBA Gene on Jeju Island, Korea
    Article Snippet: The synthesized cDNA was amplified using primers (Forward, 5'-ATG GGG CAG ATC GAG TGG GC-3'and Reverse, 5'-TCA CAC GAC CTC GTC GGT CA-3'; CYBA accession number, NM_000101) in a 50-µL PCR reaction containing 0.2 mM dNTP mix, 0.4 µM primer, and 1.25 units of Taq polymerase (Promega). .. The RT-PCR products were separated in a 2% agarose gel and stained with ethidium bromide for visualization.

    Article Title: Authentication of beef, carabeef, chevon, mutton and pork by a PCR-RFLP assay of mitochondrial cytb gene
    Article Snippet: The PCR was set up in a volume of 50 μL containing 5 μL of 10X assay buffer (160 m M (NH4)2SO4, 670 m M Tris-HCl, pH 8.8, 0.1 % tween-20, 25 m M MgCl2 , Bioron-GmbH, Ludwigshafen, Germany), 1 μL (200 μ M each) of dNTP mix (pH 7.5, Promega, Madison, Wisconsin, USA), 1 μL (25 pmol) each of forward and reverse primer (IDT, Iowa, USA), 2.5U Taq DNA polymerase (Bioron-GmbH, Ludwigshafen, Germany), 50 ng of purified DNA and nuclease free water (Merck, Darmstadt, Germany). .. The amplicons were separated by agarose gel electrophoresis (2 %, prepared in 0.5X TBE buffer, 50 V for 1.5 h) and their size determined by comparing with 100 bp DNA ladder (M/s Bangalore Genei, Bangalore, India).

    Article Title: Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia
    Article Snippet: The 25 µ L reaction mixtures included 5 µ L of 5x PCR buffer, 2.0 mM MgCl2 , 0.4 μ M of each primer pair, 0.2 mM of dNTP mix, 1.25 U of Taq DNA polymerase (Promega Corporation, USA), and 5 μ L of DNA template. .. Electrophoresis was then carried out using 2% agarose gel in 1x TBE buffer.

    Article Title: Genome-wide development of transposable elements-based markers in foxtail millet and construction of an integrated database
    Article Snippet: The DNA was quantified using 0.8% agarose gel by comparing with λ-Hin dIII DNA (Fermentas) as marker. .. The PCR amplification reactions were performed in a 25 µl reaction volume containing 100 ng of genomic DNA, 1× Taq buffer, 2 mM of MgCl2 , 0.2 mM dNTP mix (Promega), 0.5 mM each of the forward and reverse primers and four units of Taq polymerase (Biotools).

    Electrophoresis:

    Article Title: Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia
    Article Snippet: The 25 µ L reaction mixtures included 5 µ L of 5x PCR buffer, 2.0 mM MgCl2 , 0.4 μ M of each primer pair, 0.2 mM of dNTP mix, 1.25 U of Taq DNA polymerase (Promega Corporation, USA), and 5 μ L of DNA template. .. Electrophoresis was then carried out using 2% agarose gel in 1x TBE buffer.

    Quantitation Assay:

    Article Title: A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W]A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W] [OA]
    Article Snippet: Paragraph title: Real-Time PCR Quantitation and RNA Gel Blotting ... A cDNA template for PCR was generated with a single RT reaction: 1 μg of RNA was mixed with RNase-free water, 2.5 μL of random hexamers (0.1 μg/μL; Invitrogen), and 1 μL of deoxynucleotide triphosphate mix (10 μM) to a final volume of 12 μL, then incubated at 65°C for 5 min. To this, 4 μL of 5× first-strand buffer, 2 μL of 0.1 M DTT, 1 μL of RNasin Plus RNase inhibitor, and 40 units/μL (Promega) and 1 μL (200 units) of SuperScript II (Invitrogen) were added; the mixture was incubated at 42°C for 60 min. PCR was performed in a 10-μL reaction volume.

    Spectrophotometry:

    Article Title: Rapid DNA Amplification Using a Battery-Powered Thin-Film Resistive Thermocycler
    Article Snippet: Ultraspec 3000 spectrophotometer (Pharmacia, Peapack NJ). .. EDAS 290 digital camera/stand, Kodak, Rochester NY. dNTP mix, PR-U1511, Promega Corp., Madison, WI.

    Article Title: The Role of Hepatocyte Nuclear Factor 4-Alpha in Perfluorooctanoic Acid- and Perfluorooctanesulfonic Acid-Induced Hepatocellular Dysfunction
    Article Snippet: A NanoDrop 1000 spectrophotometer (Thermo Scientific) was used to determine the quantity and quality of the RNA collected. .. Complementary DNA (cDNA) was prepared from 1 mg of RNA using deoxynucleotide triphosphate mix, random primers, RNase Inhibitor, reaction buffer and reverse transcriptase as recommended by the manufacturer (Promega, Madison, WI).

    Activation Assay:

    Article Title: A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W]A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W] [OA]
    Article Snippet: A cDNA template for PCR was generated with a single RT reaction: 1 μg of RNA was mixed with RNase-free water, 2.5 μL of random hexamers (0.1 μg/μL; Invitrogen), and 1 μL of deoxynucleotide triphosphate mix (10 μM) to a final volume of 12 μL, then incubated at 65°C for 5 min. To this, 4 μL of 5× first-strand buffer, 2 μL of 0.1 M DTT, 1 μL of RNasin Plus RNase inhibitor, and 40 units/μL (Promega) and 1 μL (200 units) of SuperScript II (Invitrogen) were added; the mixture was incubated at 42°C for 60 min. PCR was performed in a 10-μL reaction volume. .. The reaction was initiated by activation of the polymerase at 95°C for 10 min, followed by 45 two-step amplification cycles consisting of 15 s of denaturation at 95°C and a 60-s extension at 60°C.

    DNA Purification:

    Article Title: Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia
    Article Snippet: DNA extraction was carried out using Wizard™ Genomic DNA Purification Kit (Promega Corporation, USA) following the manufacturer's instructions prior to specific PCR amplification. .. The 25 µ L reaction mixtures included 5 µ L of 5x PCR buffer, 2.0 mM MgCl2 , 0.4 μ M of each primer pair, 0.2 mM of dNTP mix, 1.25 U of Taq DNA polymerase (Promega Corporation, USA), and 5 μ L of DNA template.

    Marker:

    Article Title: A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W]A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway [W] [OA]
    Article Snippet: To measure transcripts encoded by JA marker genes, TaqMan probe and primer assays were obtained from Applied Biosystems (custom assays): AOS (At5g42650, assay At02314438_s1); OPR3 (At2g06050, assay At02232505_m1); and VSP2 (At5g24770, assay At02304127_g1). .. A cDNA template for PCR was generated with a single RT reaction: 1 μg of RNA was mixed with RNase-free water, 2.5 μL of random hexamers (0.1 μg/μL; Invitrogen), and 1 μL of deoxynucleotide triphosphate mix (10 μM) to a final volume of 12 μL, then incubated at 65°C for 5 min. To this, 4 μL of 5× first-strand buffer, 2 μL of 0.1 M DTT, 1 μL of RNasin Plus RNase inhibitor, and 40 units/μL (Promega) and 1 μL (200 units) of SuperScript II (Invitrogen) were added; the mixture was incubated at 42°C for 60 min. PCR was performed in a 10-μL reaction volume.

    Article Title: Genome-wide development of transposable elements-based markers in foxtail millet and construction of an integrated database
    Article Snippet: The DNA was quantified using 0.8% agarose gel by comparing with λ-Hin dIII DNA (Fermentas) as marker. .. The PCR amplification reactions were performed in a 25 µl reaction volume containing 100 ng of genomic DNA, 1× Taq buffer, 2 mM of MgCl2 , 0.2 mM dNTP mix (Promega), 0.5 mM each of the forward and reverse primers and four units of Taq polymerase (Biotools).

    Staining:

    Article Title: Genetic Analysis of 10 Unrelated Korean Families with p22-phox-deficient Chronic Granulomatous Disease: An Unusually Identical Mutation of the CYBA Gene on Jeju Island, Korea
    Article Snippet: The synthesized cDNA was amplified using primers (Forward, 5'-ATG GGG CAG ATC GAG TGG GC-3'and Reverse, 5'-TCA CAC GAC CTC GTC GGT CA-3'; CYBA accession number, NM_000101) in a 50-µL PCR reaction containing 0.2 mM dNTP mix, 0.4 µM primer, and 1.25 units of Taq polymerase (Promega). .. The RT-PCR products were separated in a 2% agarose gel and stained with ethidium bromide for visualization.

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    Promega dntp master mix
    Dntp Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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