dnase treatment  (Jena Bioscience)


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    Jena Bioscience dnase treatment
    Dnase Treatment, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase treatment/product/Jena Bioscience
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase treatment - by Bioz Stars, 2021-07
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Tyramine receptor drives olfactory response to (E)-2-decenal in the stink bug Halyomorpha halys
    Article Snippet: Synthesis of dsRNA and H. halys treatment For RNAi silencing, HhTAR1 and LacZ (control) amplicons, 400-500 bp long, were generated by PCR using primers with 5’ extensions containing T7 promoters ( ). .. These products were cloned into pJET 1.2 vector (Thermo Fisher Scientific) and then used as templates for in vitro dsRNA synthesis performed by T7 RNA Polymerase (Jena Bioscience, Jena, Germany), according to the manufacturer’s protocol. .. After one hour of synthesis at 37 °C, a DNase I (Thermo Fisher Scientific) treatment was performed and the dsRNA was clean up by ammonium acetate precipitation ( ).

    Plasmid Preparation:

    Article Title: Tyramine receptor drives olfactory response to (E)-2-decenal in the stink bug Halyomorpha halys
    Article Snippet: Synthesis of dsRNA and H. halys treatment For RNAi silencing, HhTAR1 and LacZ (control) amplicons, 400-500 bp long, were generated by PCR using primers with 5’ extensions containing T7 promoters ( ). .. These products were cloned into pJET 1.2 vector (Thermo Fisher Scientific) and then used as templates for in vitro dsRNA synthesis performed by T7 RNA Polymerase (Jena Bioscience, Jena, Germany), according to the manufacturer’s protocol. .. After one hour of synthesis at 37 °C, a DNase I (Thermo Fisher Scientific) treatment was performed and the dsRNA was clean up by ammonium acetate precipitation ( ).

    In Vitro:

    Article Title: Tyramine receptor drives olfactory response to (E)-2-decenal in the stink bug Halyomorpha halys
    Article Snippet: Synthesis of dsRNA and H. halys treatment For RNAi silencing, HhTAR1 and LacZ (control) amplicons, 400-500 bp long, were generated by PCR using primers with 5’ extensions containing T7 promoters ( ). .. These products were cloned into pJET 1.2 vector (Thermo Fisher Scientific) and then used as templates for in vitro dsRNA synthesis performed by T7 RNA Polymerase (Jena Bioscience, Jena, Germany), according to the manufacturer’s protocol. .. After one hour of synthesis at 37 °C, a DNase I (Thermo Fisher Scientific) treatment was performed and the dsRNA was clean up by ammonium acetate precipitation ( ).

    Article Title: Functional characterization of a novel non -coding mutation “Ghent +49A  >  G” in the iron-responsive element of L-ferritin causing hereditary hyperferritinaemia-cataract syndrome
    Article Snippet: The extent to which the non-labeled probes competed with the labeled WT probe for binding the IRP1 protein, provided a quantitative insight into IRP1/IRE binding affinity. .. The T7 in vitro transcription reaction for the labeled probes was performed in 1X transcription buffer, 0.33 mM rATP, rCTP, rGTP, 3.33 μM rUTP, 20 μM ATTO-680 aminoallyl-rUTP (Jena Bioscience), 240 units T7 RNA polymerase (EMBL Heidelberg core facility), 60 units RNasin Ribonuclease Inhibitor (Promega), and 30 mM DTT in a total reaction volume of 45 µL, using 6 µg of template DNA. .. After incubation for 4 h at 37 °C, template DNA was degraded by TURBO DNase (Ambion) treatment.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Establishment and Validation of Theileria annulata Sporozoite Ak-93 Infection in Laboratory-Reared Hyalomma anatolicum Tick Using In Vivo and In Vitro Assays
    Article Snippet: RNA extraction and cDNA synthesis Total RNA was extracted from the tick stabilate using the One Step RNA reagent (YTzol Pure RNA, Yekta Tajhiz Azma, Iran) in accordance with the manufacturer’s instruction. .. DNase treatment was done to assure the RNA response in RT-PCR assay (DNase I, Jena Bioscience, Germany). .. The cDNA synthesis and further PCR were performed using the MMuLV reverse transcriptase and PCR mix (Yekta Tajhiz Azma, Iran) according to the manufacturer’s instructions.

    Isolation:

    Article Title: Alternative promoter usage modulates miRNA-guided translation inhibition of a m6A reader in phosphate starvation
    Article Snippet: .. RNA isolation and RT-qPCRTotal RNA for RT-qPCR analysis was extracted from root, shoot and seedling using an RNA purification kit, as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.5 ug RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to genes of interest and ACT2, used for data normalization. ..

    Quantitative RT-PCR:

    Article Title: Alternative promoter usage modulates miRNA-guided translation inhibition of a m6A reader in phosphate starvation
    Article Snippet: .. RNA isolation and RT-qPCRTotal RNA for RT-qPCR analysis was extracted from root, shoot and seedling using an RNA purification kit, as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.5 ug RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to genes of interest and ACT2, used for data normalization. ..

    Purification:

    Article Title: Alternative promoter usage modulates miRNA-guided translation inhibition of a m6A reader in phosphate starvation
    Article Snippet: .. RNA isolation and RT-qPCRTotal RNA for RT-qPCR analysis was extracted from root, shoot and seedling using an RNA purification kit, as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.5 ug RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to genes of interest and ACT2, used for data normalization. ..

    Article Title: The transcription and export complex THO/TREX contributes to transcription termination in plants
    Article Snippet: Transformed protoplasts were harvested by centrifugation at 6000 xg for 1 min, and resuspended in 1X Passive Lysis buffer (Promega, E1941). .. The lysate was cleared by centrifugation and RNA was extracted using RNA purification kit as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.1 µg RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 as primer following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to transcripts of interest and firefly luciferase mRNA, used for data normalization. ..

    Synthesized:

    Article Title: Alternative promoter usage modulates miRNA-guided translation inhibition of a m6A reader in phosphate starvation
    Article Snippet: .. RNA isolation and RT-qPCRTotal RNA for RT-qPCR analysis was extracted from root, shoot and seedling using an RNA purification kit, as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.5 ug RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to genes of interest and ACT2, used for data normalization. ..

    Article Title: The transcription and export complex THO/TREX contributes to transcription termination in plants
    Article Snippet: Transformed protoplasts were harvested by centrifugation at 6000 xg for 1 min, and resuspended in 1X Passive Lysis buffer (Promega, E1941). .. The lysate was cleared by centrifugation and RNA was extracted using RNA purification kit as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.1 µg RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 as primer following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to transcripts of interest and firefly luciferase mRNA, used for data normalization. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Alternative promoter usage modulates miRNA-guided translation inhibition of a m6A reader in phosphate starvation
    Article Snippet: .. RNA isolation and RT-qPCRTotal RNA for RT-qPCR analysis was extracted from root, shoot and seedling using an RNA purification kit, as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.5 ug RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to genes of interest and ACT2, used for data normalization. ..

    Article Title: The transcription and export complex THO/TREX contributes to transcription termination in plants
    Article Snippet: Transformed protoplasts were harvested by centrifugation at 6000 xg for 1 min, and resuspended in 1X Passive Lysis buffer (Promega, E1941). .. The lysate was cleared by centrifugation and RNA was extracted using RNA purification kit as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.1 µg RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 as primer following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to transcripts of interest and firefly luciferase mRNA, used for data normalization. ..

    Centrifugation:

    Article Title: The transcription and export complex THO/TREX contributes to transcription termination in plants
    Article Snippet: Transformed protoplasts were harvested by centrifugation at 6000 xg for 1 min, and resuspended in 1X Passive Lysis buffer (Promega, E1941). .. The lysate was cleared by centrifugation and RNA was extracted using RNA purification kit as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.1 µg RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 as primer following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to transcripts of interest and firefly luciferase mRNA, used for data normalization. ..

    Luciferase:

    Article Title: The transcription and export complex THO/TREX contributes to transcription termination in plants
    Article Snippet: Transformed protoplasts were harvested by centrifugation at 6000 xg for 1 min, and resuspended in 1X Passive Lysis buffer (Promega, E1941). .. The lysate was cleared by centrifugation and RNA was extracted using RNA purification kit as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment. cDNA was synthesized from 0.1 µg RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 as primer following the manufacturer’s instructions. qPCR analysis was performed using SYBR select Master Mix (Applied Biosystems, 4472908) with primer pairs specific to transcripts of interest and firefly luciferase mRNA, used for data normalization. ..

    Labeling:

    Article Title: Functional characterization of a novel non -coding mutation “Ghent +49A  >  G” in the iron-responsive element of L-ferritin causing hereditary hyperferritinaemia-cataract syndrome
    Article Snippet: The extent to which the non-labeled probes competed with the labeled WT probe for binding the IRP1 protein, provided a quantitative insight into IRP1/IRE binding affinity. .. The T7 in vitro transcription reaction for the labeled probes was performed in 1X transcription buffer, 0.33 mM rATP, rCTP, rGTP, 3.33 μM rUTP, 20 μM ATTO-680 aminoallyl-rUTP (Jena Bioscience), 240 units T7 RNA polymerase (EMBL Heidelberg core facility), 60 units RNasin Ribonuclease Inhibitor (Promega), and 30 mM DTT in a total reaction volume of 45 µL, using 6 µg of template DNA. .. After incubation for 4 h at 37 °C, template DNA was degraded by TURBO DNase (Ambion) treatment.

    Article Title: Design and application of circular RNAs with protein-sponge function
    Article Snippet: In vitro transcription and circularization of short circRNAs RNAs were synthesized by in vitro transcription, using double-stranded DNA-oligonucleotide templates and the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB), and labeling internally by incorporation of azide-modified UTP analog (5-Azido-C3-UTP, 33%; Jena Bioscience), followed by RQ1 DNase treatment (10 U per 100 μl-reaction; Promega) and Sephadex column purification (Sigma-Aldrich). .. For circularization, T4 RNA ligase (100 U per 100 μl-reaction; Thermo Fisher Scientific) was used, followed by biotin labeling with copper-free Click Chemistry (DBCO-PEG4-Biotin conjugate; Jena Bioscience). .. HnRNP L sponging assays: biotin pull-down and RNA immunoprecipitation (RIP) HeLa cells were lysed in RIPA buffer [50 mM Tris–Cl pH 7.4, 150 mM NaCl, 5 mM EDTA, 1% NP-40 (v/v)].

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  • 86
    Jena Bioscience dnase treatment
    Dnase Treatment, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase treatment/product/Jena Bioscience
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase treatment - by Bioz Stars, 2021-07
    86/100 stars
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