dnase i protease inhibitor cocktail set iii  (Millipore)


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    Name:
    DNase I
    Description:
    Deoxyribonuclease I DNase I is an endonuclease isolated from bovine pancreas that digests double and single stranded DNA into oligo and mononucleotides Amplification Grade DNase I has been purified to remove RNase activity and is suitable for eliminating DNA from RNA preparations prior to sensitive applications such as RT PCR Reverse Transcriptase Polymerase Chain Reaction DNase I digests double and single stranded DNA into oligo and mononucleotides Using the Reaction Buffer provided DNA is removed from RNA preparations in a 15 minute digestion at room temperature The DNase I is then inactivated by heating with the Stop Solution Heating also denatures hairpins in the RNA so the RNA can be used directly in reverse transcription No current RNA isolation procedure removes 100 of the DNA Many commercial DNase I formulations are contaminated with residual RNases This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription Laboratory comparisons have shown that Sigma s Amplification Grade DNase I demonstrates lower RNase activity than that from several leading molecular biology product suppliers
    Catalog Number:
    ampd1
    Price:
    None
    Applications:
    Amplification grade DNase I has been used for the digestion of DNA during isolation and purification of RNA. The purified RNA can be used for the synthesis of cDNA using RNA reverse transcriptase.
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    Structured Review

    Millipore dnase i protease inhibitor cocktail set iii
    DNase I
    Deoxyribonuclease I DNase I is an endonuclease isolated from bovine pancreas that digests double and single stranded DNA into oligo and mononucleotides Amplification Grade DNase I has been purified to remove RNase activity and is suitable for eliminating DNA from RNA preparations prior to sensitive applications such as RT PCR Reverse Transcriptase Polymerase Chain Reaction DNase I digests double and single stranded DNA into oligo and mononucleotides Using the Reaction Buffer provided DNA is removed from RNA preparations in a 15 minute digestion at room temperature The DNase I is then inactivated by heating with the Stop Solution Heating also denatures hairpins in the RNA so the RNA can be used directly in reverse transcription No current RNA isolation procedure removes 100 of the DNA Many commercial DNase I formulations are contaminated with residual RNases This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription Laboratory comparisons have shown that Sigma s Amplification Grade DNase I demonstrates lower RNase activity than that from several leading molecular biology product suppliers
    https://www.bioz.com/result/dnase i protease inhibitor cocktail set iii/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i protease inhibitor cocktail set iii - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: DNA and a CpG Oligonucleotide Derived from Babesia bovis Are Mitogenic for Bovine B Cells
    Article Snippet: .. CM antigen was incubated for 2 h at 37°C at a concentration of 1 mg of protein per ml of DNase I buffer containing 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2 , and 0.5 mg of BSA per ml with a final concentration of 1 mg of DNase I (Sigma) per ml. .. Purified DNA (150 to 200 μg/ml) was similarly treated with DNase for 2 h at 37°C and then stored at −20°C until use.

    Incubation:

    Article Title: DNA and a CpG Oligonucleotide Derived from Babesia bovis Are Mitogenic for Bovine B Cells
    Article Snippet: .. CM antigen was incubated for 2 h at 37°C at a concentration of 1 mg of protein per ml of DNase I buffer containing 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2 , and 0.5 mg of BSA per ml with a final concentration of 1 mg of DNase I (Sigma) per ml. .. Purified DNA (150 to 200 μg/ml) was similarly treated with DNase for 2 h at 37°C and then stored at −20°C until use.

    Article Title: A critical role for the chromatin remodeller CHD7 in anterior mesoderm during cardiovascular development
    Article Snippet: .. 4.6 DNase I hypersensitivity assay Individual E11.5 hearts were dissected in cold PBS and incubated in 0.05% Trypsin-EDTA (Life Technologies) containing 10 µg/ml DNase I (Sigma) at 37 °C for 45 min. Trypsin was inhibited with 10% FBS (Life Technologies) and samples passed through a 23G needle three times. .. Cells were pelleted by centrifugation and nuclei released in lysis buffer (10 mM Tris pH7.4, 10 mM NaCl, 3 mM MgCl2 , 0.1% NP40).

    Article Title: Obesity Risk Gene TMEM18 Encodes a Sequence-Specific DNA-Binding Protein
    Article Snippet: .. Dnase I treatment of the matrix was done as follows: the matrix was washed three times with 10 mM Tris pH 7.5, 0.2 M NaCl and incubated with 10 mg/ml of Dnase I (Sigma) in 10 mM Hepes pH 7.9, 10 mM KCl, 1.5 mM MgCl2 for 15 minutes at room temperature (RT). ..

    Article Title: Polarization of macrophages in the tumor microenvironment is influenced by EGFR signaling within colon cancer cells
    Article Snippet: .. Briefly, colon tissues were cut into small pieces (1-2 mm) and incubated in 10 mL PRMI 1640 medium with 10 mM HEPES and 5% fetal bovine serum containing 10 mg (1 mg/mL) collagenase D (Sigma-Aldrich), 10 mg (1 mg/mL) dispase II (Roche, Germany), and 100 μL 10 mg/ml DNase I (100 μg/mL) (Sigma-Aldrich) for 30-45 min in a shaking incubator at 37°C. .. For Ana-1 and BMDM cells, single-cell suspensions from mouse colon tissues were incubated with APC-anti-mouse CD11b and FITC-anti-mouse F4/80 (Affymetrix eBioscience, USA), or APC-anti-mouse F4/80 and FITC-anti-mouse CD206 (MMR) (Biolegend, San Diego, CA) antibodies.

    Lysis:

    Article Title: GATA-1 Utilizes Ikaros and Polycomb Repressive Complex 2 To Suppress Hes1 and To Promote Erythropoiesis
    Article Snippet: .. For DNase I, RNase I, and ethidium bromide (EtBr) IP assays, DNase (1 μg/ml; Sigma), RNase (1 μg/ml; Sigma), and EtBr (50 μg/ml; Sigma) were added to the lysis buffer. ..

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    Millipore dnase i protease inhibitor cocktail set iii
    Dnase I Protease Inhibitor Cocktail Set Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i protease inhibitor cocktail set iii/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i protease inhibitor cocktail set iii - by Bioz Stars, 2020-09
    99/100 stars
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