dnase i inhibitor  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dnase i inhibitor
    Dnase I Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i inhibitor/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i inhibitor - by Bioz Stars, 2020-04
    79/100 stars

    Related Products / Commonly Used Together

    dnase i
    rna
    dnase i activity

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    Related Articles

    RNA Extraction:

    Article Title: Evaluating the Involvement of Alternative Sigma Factors SigF and SigG in Clostridium perfringens Sporulation and Enterotoxin Synthesis ▿
    Article Snippet: Paragraph title: RNA extraction and RT-PCR. ... All phenol-extracted samples were then treated with 2 U of DNase I (Ambion) at 37°C for 30 min. To stop this DNase I activity, a DNase I inhibitor (Ambion) was added to each reaction tube.

    Centrifugation:

    Article Title: Evaluating the Involvement of Alternative Sigma Factors SigF and SigG in Clostridium perfringens Sporulation and Enterotoxin Synthesis ▿
    Article Snippet: After centrifugation of each removed culture aliquot, total C. perfringens RNA was extracted from the pelleted cells by using saturated phenol (Fisher Scientific), as described in a previous study ( ). .. All phenol-extracted samples were then treated with 2 U of DNase I (Ambion) at 37°C for 30 min. To stop this DNase I activity, a DNase I inhibitor (Ambion) was added to each reaction tube.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Evaluating the Involvement of Alternative Sigma Factors SigF and SigG in Clostridium perfringens Sporulation and Enterotoxin Synthesis ▿
    Article Snippet: Paragraph title: RNA extraction and RT-PCR. ... All phenol-extracted samples were then treated with 2 U of DNase I (Ambion) at 37°C for 30 min. To stop this DNase I activity, a DNase I inhibitor (Ambion) was added to each reaction tube.

    Activity Assay:

    Article Title: Evaluating the Involvement of Alternative Sigma Factors SigF and SigG in Clostridium perfringens Sporulation and Enterotoxin Synthesis ▿
    Article Snippet: .. All phenol-extracted samples were then treated with 2 U of DNase I (Ambion) at 37°C for 30 min. To stop this DNase I activity, a DNase I inhibitor (Ambion) was added to each reaction tube. ..

    Expressing:

    Article Title: Evaluating the Involvement of Alternative Sigma Factors SigF and SigG in Clostridium perfringens Sporulation and Enterotoxin Synthesis ▿
    Article Snippet: To conduct a time course study of cpe gene expression during sporulation, a starter culture of wild-type SM101 was first grown overnight at 37°C in FTG. .. All phenol-extracted samples were then treated with 2 U of DNase I (Ambion) at 37°C for 30 min. To stop this DNase I activity, a DNase I inhibitor (Ambion) was added to each reaction tube.

    Polymerase Chain Reaction:

    Article Title: Evaluating the Involvement of Alternative Sigma Factors SigF and SigG in Clostridium perfringens Sporulation and Enterotoxin Synthesis ▿
    Article Snippet: All phenol-extracted samples were then treated with 2 U of DNase I (Ambion) at 37°C for 30 min. To stop this DNase I activity, a DNase I inhibitor (Ambion) was added to each reaction tube. .. Briefly, each RNA sample (100 ng) was reverse transcribed to cDNA at 45°C for 1 h, and the cDNA was then used as a template for PCR with primers targeting cpe sequences or spo0A (as a control housekeeping gene).

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  • 99
    Thermo Fisher deoxyribonuclease i
    Deoxyribonuclease I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxyribonuclease i/product/Thermo Fisher
    Average 99 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    deoxyribonuclease i - by Bioz Stars, 2020-04
    99/100 stars
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