dnase i fitc  (Thermo Fisher)


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  • 80

    Structured Review

    Thermo Fisher dnase i fitc
    The spatial distribution of actin in RBC ghosts revealed by fluorescent probes of phalloidin-rhodamine and DNase <t>I–FITC.</t> The spatial distribution of the fluorescent probes was analyzed via a three-dimensional optical sectioning by a confocal inverted laser scanning microscope. ( A ) Optical sections ( a–h , 0.6 μm apart) of RBC ghost labeled by phalloidin-rhodamine (4.4 μM). Each square of the gallery of images possesses a dimension of 9.5 μm × 9.5 μm. ( B ) The distribution of phalloidin-rhodamine and DNase I–FITC in RBC ghosts in an optical section taken at the middle (half thickness) of the RBC ghost. Inset , The separate distribution of phalloidin-rhodamine ( red ) and <t>DNase</t> I ( green ) in a RBC ghost that was labeled with both phalloidin-rhodamine and DNase I–FITC. Bars: ( A ) 5 μm; ( B ) 10 μm.
    Dnase I Fitc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i fitc/product/Thermo Fisher
    Average 80 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    dnase i fitc - by Bioz Stars, 2020-03
    80/100 stars

    Related Products / Commonly Used Together

    phalloidin-rhodamine
    rbc ghosts
    kcl solution
    g-actin

    Images

    1) Product Images from "Mechanical Fluctuations of the Membrane-Skeleton Are Dependent on F-Actin ATPase in Human Erythrocytes "

    Article Title: Mechanical Fluctuations of the Membrane-Skeleton Are Dependent on F-Actin ATPase in Human Erythrocytes

    Journal: The Journal of Cell Biology

    doi:

    The spatial distribution of actin in RBC ghosts revealed by fluorescent probes of phalloidin-rhodamine and DNase I–FITC. The spatial distribution of the fluorescent probes was analyzed via a three-dimensional optical sectioning by a confocal inverted laser scanning microscope. ( A ) Optical sections ( a–h , 0.6 μm apart) of RBC ghost labeled by phalloidin-rhodamine (4.4 μM). Each square of the gallery of images possesses a dimension of 9.5 μm × 9.5 μm. ( B ) The distribution of phalloidin-rhodamine and DNase I–FITC in RBC ghosts in an optical section taken at the middle (half thickness) of the RBC ghost. Inset , The separate distribution of phalloidin-rhodamine ( red ) and DNase I ( green ) in a RBC ghost that was labeled with both phalloidin-rhodamine and DNase I–FITC. Bars: ( A ) 5 μm; ( B ) 10 μm.
    Figure Legend Snippet: The spatial distribution of actin in RBC ghosts revealed by fluorescent probes of phalloidin-rhodamine and DNase I–FITC. The spatial distribution of the fluorescent probes was analyzed via a three-dimensional optical sectioning by a confocal inverted laser scanning microscope. ( A ) Optical sections ( a–h , 0.6 μm apart) of RBC ghost labeled by phalloidin-rhodamine (4.4 μM). Each square of the gallery of images possesses a dimension of 9.5 μm × 9.5 μm. ( B ) The distribution of phalloidin-rhodamine and DNase I–FITC in RBC ghosts in an optical section taken at the middle (half thickness) of the RBC ghost. Inset , The separate distribution of phalloidin-rhodamine ( red ) and DNase I ( green ) in a RBC ghost that was labeled with both phalloidin-rhodamine and DNase I–FITC. Bars: ( A ) 5 μm; ( B ) 10 μm.

    Techniques Used: Laser-Scanning Microscopy, Labeling

    Related Articles

    Incubation:

    Article Title: Mechanical Fluctuations of the Membrane-Skeleton Are Dependent on F-Actin ATPase in Human Erythrocytes
    Article Snippet: .. The RBC ghosts were washed twice with a KCl solution, and then incubated with 4.4 μM phalloidin-rhodamine (Molecular Probes, Inc., Eugene, OR) alone or with a combination of 4.4 μM phalloidin-rhodamine and 35 nM DNase I–FITC (Molecular Probes, Inc.). .. The incubation with the fluorescent probes was carried out for 20 min followed by an extensive fivefold slow wash of the RBC ghosts with a KCl solution.

    Article Title: Gelsolin overexpression alters actin dynamics and tyrosine phosphorylation of lipid raft-associated proteins in Jurkat T cells
    Article Snippet: .. For determination of G-actin, samples were incubated with 5 μg DNase I-FITC (Molecular Probes) for 2 h at RT in the dark. .. After incubation, samples were washed twice with 1x PBS.

    Laser-Scanning Microscopy:

    Article Title: Mechanical Fluctuations of the Membrane-Skeleton Are Dependent on F-Actin ATPase in Human Erythrocytes
    Article Snippet: The RBC ghosts were washed twice with a KCl solution, and then incubated with 4.4 μM phalloidin-rhodamine (Molecular Probes, Inc., Eugene, OR) alone or with a combination of 4.4 μM phalloidin-rhodamine and 35 nM DNase I–FITC (Molecular Probes, Inc.). .. The spatial distribution of the fluorescent probes was analyzed via a three-dimensional optical sectioning using a confocal inverted laser scanning microscope (LSM 410; Carl Zeiss Inc. , Thornwood, NY).

    Quantitation Assay:

    Article Title: Gelsolin overexpression alters actin dynamics and tyrosine phosphorylation of lipid raft-associated proteins in Jurkat T cells
    Article Snippet: Paragraph title: 2.3. Quantitation of F- and G-actin ... For determination of G-actin, samples were incubated with 5 μg DNase I-FITC (Molecular Probes) for 2 h at RT in the dark.

    Labeling:

    Article Title: Mechanical Fluctuations of the Membrane-Skeleton Are Dependent on F-Actin ATPase in Human Erythrocytes
    Article Snippet: The labeling was carried out on RBC ghosts adhered to the cover-glass, prepared by the procedure described above. .. The RBC ghosts were washed twice with a KCl solution, and then incubated with 4.4 μM phalloidin-rhodamine (Molecular Probes, Inc., Eugene, OR) alone or with a combination of 4.4 μM phalloidin-rhodamine and 35 nM DNase I–FITC (Molecular Probes, Inc.).