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MACHEREY NAGEL dnase i digestion
Dnase I Digestion, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dnase i digestion/product/MACHEREY NAGEL
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dnase i digestion - by Bioz Stars, 2020-04
92/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Aphid Acceptance of Barley Exposed to Volatile Phytochemicals Differs Between Plants Exposed in Daylight and Darkness
Article Snippet: From the plant material collected in 2006, total RNA was extracted from the tissue using NucleoSpin® RNA Plant kit which includes a DNase I digestion (Macherey-Nagel, Düren, Germany). .. Real time RT-PCR was performed and analysed with MyiQ™ Single-Color Real-Time PCR Detection System (Bio-Rad, California, USA) using the iScript™ One-Step RT-PCR Kit With SYBR® Green (Bio-Rad).

Article Title: PPARγ deacetylation dissociates thiazolidinedione’s metabolic benefits from its adverse effects
Article Snippet: After phase separation through the addition of chloroform, RNA was isolated using the NucleoSpin RNA Kit with DNase I digestion (Macherey-Nagel, Thermo Fisher Scientific). .. Quantitative real-time PCR (qPCR) was performed on a Bio-Rad CFX96 Real-Time PCR system by using the GoTaq qPCR Master Mix (Promega).

RNA Extraction:

Article Title: Arabidopsis AtCUL3a and AtCUL3b Form Complexes with Members of the BTB/POZ-MATH Protein Family 1
Article Snippet: .. Total RNA extraction and DNase I digestion were done using a NucleoSpin RNA Plant kit (Macherey-Nagel, Duren, Germany). .. RT-PCR was done using a One-Step RT-PCR kit (Qiagen, Valencia, CA).

Amplification:

Article Title: Directing Cardiomyogenic Differentiation of Human Pluripotent Stem Cells by Plasmid-Based Transient Overexpression of Cardiac Transcription Factors
Article Snippet: Total RNA was obtained by dissolving cells in Trizol® Reagent (Invitrogen, Karlsruhe, Germany) followed by isolation with a Nucleospin® RNA II Kit, including DNase I digestion, according to the manufacturer's instructions (Macherey Nagel, Düren, Germany). .. For conventional RT-PCR, 1 μL of cDNA was amplified by PCR with GoTaq polymerase (Promega, Madison, WI).

Synthesized:

Article Title: PPARγ deacetylation dissociates thiazolidinedione’s metabolic benefits from its adverse effects
Article Snippet: After phase separation through the addition of chloroform, RNA was isolated using the NucleoSpin RNA Kit with DNase I digestion (Macherey-Nagel, Thermo Fisher Scientific). .. To isolate a small amount of RNA from Tregs, the RNeasy Micro Kit (QIAGEN) was used instead. cDNA was synthesized from 1 μg total RNA by using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

Isolation:

Article Title: Directing Cardiomyogenic Differentiation of Human Pluripotent Stem Cells by Plasmid-Based Transient Overexpression of Cardiac Transcription Factors
Article Snippet: .. Total RNA was obtained by dissolving cells in Trizol® Reagent (Invitrogen, Karlsruhe, Germany) followed by isolation with a Nucleospin® RNA II Kit, including DNase I digestion, according to the manufacturer's instructions (Macherey Nagel, Düren, Germany). .. RNA quality was assessed by a Bioanalyzer 2100 (Agilent, Boeblingen, Germany).

Article Title: Ragweed (Ambrosia artemisiifolia) pollen allergenicity: SuperSAGE transcriptomic analysis upon elevated CO2 and drought stress
Article Snippet: The isolation was carried out by GenXPro GmbH (Frankfurt, Germany) using the InviTrap® Spin Plant RNA Mini Kit (STRATEC Molecular GmbH, Berlin, Germany). .. Purification of total RNA after DNAse I digestion was carried out with MACHEREY-NAGEL “NucleoSpinRNA Clean-up XS-Kit (MACHEREY-NAGEL, Düren, Germany).

Article Title: PPARγ deacetylation dissociates thiazolidinedione’s metabolic benefits from its adverse effects
Article Snippet: .. After phase separation through the addition of chloroform, RNA was isolated using the NucleoSpin RNA Kit with DNase I digestion (Macherey-Nagel, Thermo Fisher Scientific). .. To isolate a small amount of RNA from Tregs, the RNeasy Micro Kit (QIAGEN) was used instead. cDNA was synthesized from 1 μg total RNA by using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

Quantitative RT-PCR:

Article Title: Directing Cardiomyogenic Differentiation of Human Pluripotent Stem Cells by Plasmid-Based Transient Overexpression of Cardiac Transcription Factors
Article Snippet: Paragraph title: RNA isolation and quantitative RT-PCR ... Total RNA was obtained by dissolving cells in Trizol® Reagent (Invitrogen, Karlsruhe, Germany) followed by isolation with a Nucleospin® RNA II Kit, including DNase I digestion, according to the manufacturer's instructions (Macherey Nagel, Düren, Germany).

Article Title: Aphid Acceptance of Barley Exposed to Volatile Phytochemicals Differs Between Plants Exposed in Daylight and Darkness
Article Snippet: Paragraph title: Real time RT-PCR. ... From the plant material collected in 2006, total RNA was extracted from the tissue using NucleoSpin® RNA Plant kit which includes a DNase I digestion (Macherey-Nagel, Düren, Germany).

Purification:

Article Title: Ragweed (Ambrosia artemisiifolia) pollen allergenicity: SuperSAGE transcriptomic analysis upon elevated CO2 and drought stress
Article Snippet: .. Purification of total RNA after DNAse I digestion was carried out with MACHEREY-NAGEL “NucleoSpinRNA Clean-up XS-Kit (MACHEREY-NAGEL, Düren, Germany). .. The quality of total RNA was checked on a Bioanalyzer with a 2100 expert Plant RNA Nano chip (Agilent Technologies, Waldbronn, Germany).

SYBR Green Assay:

Article Title: Aphid Acceptance of Barley Exposed to Volatile Phytochemicals Differs Between Plants Exposed in Daylight and Darkness
Article Snippet: From the plant material collected in 2006, total RNA was extracted from the tissue using NucleoSpin® RNA Plant kit which includes a DNase I digestion (Macherey-Nagel, Düren, Germany). .. Real time RT-PCR was performed and analysed with MyiQ™ Single-Color Real-Time PCR Detection System (Bio-Rad, California, USA) using the iScript™ One-Step RT-PCR Kit With SYBR® Green (Bio-Rad).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Directing Cardiomyogenic Differentiation of Human Pluripotent Stem Cells by Plasmid-Based Transient Overexpression of Cardiac Transcription Factors
Article Snippet: Total RNA was obtained by dissolving cells in Trizol® Reagent (Invitrogen, Karlsruhe, Germany) followed by isolation with a Nucleospin® RNA II Kit, including DNase I digestion, according to the manufacturer's instructions (Macherey Nagel, Düren, Germany). .. For conventional RT-PCR, 1 μL of cDNA was amplified by PCR with GoTaq polymerase (Promega, Madison, WI).

Article Title: Aphid Acceptance of Barley Exposed to Volatile Phytochemicals Differs Between Plants Exposed in Daylight and Darkness
Article Snippet: From the plant material collected in 2006, total RNA was extracted from the tissue using NucleoSpin® RNA Plant kit which includes a DNase I digestion (Macherey-Nagel, Düren, Germany). .. Real time RT-PCR was performed and analysed with MyiQ™ Single-Color Real-Time PCR Detection System (Bio-Rad, California, USA) using the iScript™ One-Step RT-PCR Kit With SYBR® Green (Bio-Rad).

Article Title: Arabidopsis AtCUL3a and AtCUL3b Form Complexes with Members of the BTB/POZ-MATH Protein Family 1
Article Snippet: Paragraph title: RT-PCR Analysis ... Total RNA extraction and DNase I digestion were done using a NucleoSpin RNA Plant kit (Macherey-Nagel, Duren, Germany).

Expressing:

Article Title: Aphid Acceptance of Barley Exposed to Volatile Phytochemicals Differs Between Plants Exposed in Daylight and Darkness
Article Snippet: From the plant material collected in 2006, total RNA was extracted from the tissue using NucleoSpin® RNA Plant kit which includes a DNase I digestion (Macherey-Nagel, Düren, Germany). .. The constitutively expressed gene actin2 ( : ACT2.FOR; 5′-TTCTCGACTCTGGTGATGGTGT-3′; ACT2.REV; 5′-CAAGCTTCTCCTTGATGTCCCT-3′) was used as a reference gene to ensure normalization of expression levels.

Article Title: PPARγ deacetylation dissociates thiazolidinedione’s metabolic benefits from its adverse effects
Article Snippet: After phase separation through the addition of chloroform, RNA was isolated using the NucleoSpin RNA Kit with DNase I digestion (Macherey-Nagel, Thermo Fisher Scientific). .. Relative gene expression levels were calculated using the ΔΔCt method with TBP or Cyclophilin A as the reference genes.

Polymerase Chain Reaction:

Article Title: Directing Cardiomyogenic Differentiation of Human Pluripotent Stem Cells by Plasmid-Based Transient Overexpression of Cardiac Transcription Factors
Article Snippet: Total RNA was obtained by dissolving cells in Trizol® Reagent (Invitrogen, Karlsruhe, Germany) followed by isolation with a Nucleospin® RNA II Kit, including DNase I digestion, according to the manufacturer's instructions (Macherey Nagel, Düren, Germany). .. For conventional RT-PCR, 1 μL of cDNA was amplified by PCR with GoTaq polymerase (Promega, Madison, WI).

Lysis:

Article Title: Ragweed (Ambrosia artemisiifolia) pollen allergenicity: SuperSAGE transcriptomic analysis upon elevated CO2 and drought stress
Article Snippet: In detail: 20–30 mg pollen was added to 900 μl lysis solution DCT + 10 μl 2-mercaptoethanol and homogenized for 2× 1 min at 30 Hz using a TissueLyser II by Retsch (QIAGEN, Hilden, Germany). .. Purification of total RNA after DNAse I digestion was carried out with MACHEREY-NAGEL “NucleoSpinRNA Clean-up XS-Kit (MACHEREY-NAGEL, Düren, Germany).

Chromatin Immunoprecipitation:

Article Title: Ragweed (Ambrosia artemisiifolia) pollen allergenicity: SuperSAGE transcriptomic analysis upon elevated CO2 and drought stress
Article Snippet: Purification of total RNA after DNAse I digestion was carried out with MACHEREY-NAGEL “NucleoSpinRNA Clean-up XS-Kit (MACHEREY-NAGEL, Düren, Germany). .. The quality of total RNA was checked on a Bioanalyzer with a 2100 expert Plant RNA Nano chip (Agilent Technologies, Waldbronn, Germany).

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    MACHEREY NAGEL dnase i digestion
    Dnase I Digestion, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i digestion/product/MACHEREY NAGEL
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i digestion - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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