dnase free proteinase k  (Millipore)


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    Structured Review

    Millipore dnase free proteinase k
    Dnase Free Proteinase K, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free proteinase k/product/Millipore
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dnase free proteinase k - by Bioz Stars, 2020-03
    96/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Oral treatment with a zinc complex of acetylsalicylic acid prevents diabetic cardiomyopathy in a rat model of type-2 diabetes: activation of the Akt pathway
    Article Snippet: Paragraph title: Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) ... Briefly, the rehydrated sections were digested with 20 μg/ml DNase-free Proteinase K (Sigma-Aldrich, Germany) to retrieve antigenic epitopes, and endogenous peroxidases were blocked with 3 % hydrogen peroxide (H2 O2 ).

    Article Title: An Altered Pattern of Myocardial Histopathological and Molecular Changes Underlies the Different Characteristics of Type-1 and Type-2 Diabetic Cardiac Dysfunction
    Article Snippet: Paragraph title: 2.7. Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick-End Labeling (TUNEL) Assay ... Rehydrated sections were treated with 20 μ g/mL DNase-free Proteinase-K (Sigma-Aldrich, Germany) to retrieve antigenic epitopes, followed by 3% hydrogen peroxide to quench endogenous peroxidase activity.

    Article Title: Upregulation of heme oxygenase-1 protected against brain damage induced by transient cerebral ischemia-reperfusion injury in rats
    Article Snippet: Paragraph title: Terminal deoxynucleotidyl-transferase-mediated dUTP nick end (TUNEL) staining ... Subsequently, brain tissue sections were dewaxed and rehydrated at 60°C for 2 h. These slides were incubated for 30 min at room temperature in a 20 µg/ml DNase-free proteinase K and rat terminal deoxynucleotidyl-transferase (both Sigma-Aldrich; Merck KGaA) reaction mix.

    Article Title: Differential expression of apoptosis-related factors induces the age-related apoptosis of the gracilis muscle in humans
    Article Snippet: Paragraph title: In situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) ... The dehydrated sections were treated with 20 μg/ml DNase-free proteinase K (Sigma-Aldrich Corp., St. Louis, MO, USA) to retrieve antigenic epitopes, followed by 3% H2 O2 to quench endogenous peroxidase activity.

    Nucleic Acid Electrophoresis:

    Article Title: Minichromosome Maintenance Proteins Cooperate with LANA during the G1/S Phase of the Cell Cycle To Support Viral DNA Replication
    Article Snippet: Paragraph title: Gardella gel electrophoresis. ... KSHV-positive BCBL-1 and BC3 cells depleted of MCM3 and MCM6 were loaded onto the agarose gel with a lysis plug containing DNase-free proteinase K (Sigma-Aldrich, St. Louis, MO, USA) and SDS, followed by electrophoresis in a Tris-borate-EDTA buffer.

    Agarose Gel Electrophoresis:

    Article Title: Minichromosome Maintenance Proteins Cooperate with LANA during the G1/S Phase of the Cell Cycle To Support Viral DNA Replication
    Article Snippet: .. KSHV-positive BCBL-1 and BC3 cells depleted of MCM3 and MCM6 were loaded onto the agarose gel with a lysis plug containing DNase-free proteinase K (Sigma-Aldrich, St. Louis, MO, USA) and SDS, followed by electrophoresis in a Tris-borate-EDTA buffer. .. The plugs were loaded on 0.8% Gardella gel and resolved at 108 V for 30 h. The gel was transferred onto a Hybond N+ membrane (GE Healthcare) and hybridized with 32 P-labeled TR probes to detect KSHV episome.

    Article Title: KSHV encoded LANA recruits Nucleosome Assembly Protein NAP1L1 for regulating viral DNA replication and transcription
    Article Snippet: .. KSHV-positive BCBL-1 and BC-3 cells depleted for LANA and NAP1L1 were loaded into the agarose gel with a lysis plug containing DNase-free Proteinase K (Sigma-Aldrich, St. Louis, MO, USA) and SDS followed by electrophoresis in a Tris-borate-EDTA buffer. .. The gels were run overnight at 15 V and then at 110 V for another 24 h. DNA was transferred to a Hybond N + membrane (GE Healthcare) and hybridized with 32 P-labeled TR probes to detect KSHV episome.

    Article Title: GREEN TEA POLYPHENOLS MEDIATED APOPTOSIS IN INTESTINAL EPITHELIAL CELLS BY A FADD-DEPENDENT PATHWAY
    Article Snippet: Cells were collected and lysed with 1.0ml of lysis buffer [100 mm NaCl, 10 mm Tris (pH 8.0), 25mm ethylenediaminetetraacetic acid, 0.5% sodium dodecyl sulphate (SDS), 200 µg/ml of DNase-free proteinase K (Sigma)] and treated with DNAse free RNase (40 µg/ml) in the presence of 0.1% SDS for 1h at 37 °C. .. Cells were collected and lysed with 1.0ml of lysis buffer [100 mm NaCl, 10 mm Tris (pH 8.0), 25mm ethylenediaminetetraacetic acid, 0.5% sodium dodecyl sulphate (SDS), 200 µg/ml of DNase-free proteinase K (Sigma)] and treated with DNAse free RNase (40 µg/ml) in the presence of 0.1% SDS for 1h at 37 °C.

    In Situ:

    Article Title: Differential expression of apoptosis-related factors induces the age-related apoptosis of the gracilis muscle in humans
    Article Snippet: Paragraph title: In situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) ... The dehydrated sections were treated with 20 μg/ml DNase-free proteinase K (Sigma-Aldrich Corp., St. Louis, MO, USA) to retrieve antigenic epitopes, followed by 3% H2 O2 to quench endogenous peroxidase activity.

    Fluorescence:

    Article Title: GREEN TEA POLYPHENOLS MEDIATED APOPTOSIS IN INTESTINAL EPITHELIAL CELLS BY A FADD-DEPENDENT PATHWAY
    Article Snippet: Cells were collected and lysed with 1.0ml of lysis buffer [100 mm NaCl, 10 mm Tris (pH 8.0), 25mm ethylenediaminetetraacetic acid, 0.5% sodium dodecyl sulphate (SDS), 200 µg/ml of DNase-free proteinase K (Sigma)] and treated with DNAse free RNase (40 µg/ml) in the presence of 0.1% SDS for 1h at 37 °C. .. Gels were stained with ethidium bromide and visualized by ultraviolet fluorescence and photographed with a Polaroid camera system.

    Article Title: Upregulation of heme oxygenase-1 protected against brain damage induced by transient cerebral ischemia-reperfusion injury in rats
    Article Snippet: Subsequently, brain tissue sections were dewaxed and rehydrated at 60°C for 2 h. These slides were incubated for 30 min at room temperature in a 20 µg/ml DNase-free proteinase K and rat terminal deoxynucleotidyl-transferase (both Sigma-Aldrich; Merck KGaA) reaction mix. .. The tissue slide was digitally photographed using an Olympus BX51 fluorescence microscope (magnification, ×400).

    Labeling:

    Article Title: Oral treatment with a zinc complex of acetylsalicylic acid prevents diabetic cardiomyopathy in a rat model of type-2 diabetes: activation of the Akt pathway
    Article Snippet: Briefly, the rehydrated sections were digested with 20 μg/ml DNase-free Proteinase K (Sigma-Aldrich, Germany) to retrieve antigenic epitopes, and endogenous peroxidases were blocked with 3 % hydrogen peroxide (H2 O2 ). .. The free 3ʹ-OH termini of the DNA ends were labeled with a reaction mixture of terminal deoxynucleotidyl transferase (TdT) and digoxigenin-dUTP at 37 °C for 1 h (Chemicon International, Temecula, CA, USA).

    Article Title: An Altered Pattern of Myocardial Histopathological and Molecular Changes Underlies the Different Characteristics of Type-1 and Type-2 Diabetic Cardiac Dysfunction
    Article Snippet: Paragraph title: 2.7. Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick-End Labeling (TUNEL) Assay ... Rehydrated sections were treated with 20 μ g/mL DNase-free Proteinase-K (Sigma-Aldrich, Germany) to retrieve antigenic epitopes, followed by 3% hydrogen peroxide to quench endogenous peroxidase activity.

    Article Title: Differential expression of apoptosis-related factors induces the age-related apoptosis of the gracilis muscle in humans
    Article Snippet: The dehydrated sections were treated with 20 μg/ml DNase-free proteinase K (Sigma-Aldrich Corp., St. Louis, MO, USA) to retrieve antigenic epitopes, followed by 3% H2 O2 to quench endogenous peroxidase activity. .. Free 3′-OH termini were labeled with digoxigenin-dUTP for 1 h at 37ºC utilizing a terminal deoxynucleotidyl transferase reaction mixture.

    Electrophoresis:

    Article Title: Minichromosome Maintenance Proteins Cooperate with LANA during the G1/S Phase of the Cell Cycle To Support Viral DNA Replication
    Article Snippet: .. KSHV-positive BCBL-1 and BC3 cells depleted of MCM3 and MCM6 were loaded onto the agarose gel with a lysis plug containing DNase-free proteinase K (Sigma-Aldrich, St. Louis, MO, USA) and SDS, followed by electrophoresis in a Tris-borate-EDTA buffer. .. The plugs were loaded on 0.8% Gardella gel and resolved at 108 V for 30 h. The gel was transferred onto a Hybond N+ membrane (GE Healthcare) and hybridized with 32 P-labeled TR probes to detect KSHV episome.

    Article Title: KSHV encoded LANA recruits Nucleosome Assembly Protein NAP1L1 for regulating viral DNA replication and transcription
    Article Snippet: .. KSHV-positive BCBL-1 and BC-3 cells depleted for LANA and NAP1L1 were loaded into the agarose gel with a lysis plug containing DNase-free Proteinase K (Sigma-Aldrich, St. Louis, MO, USA) and SDS followed by electrophoresis in a Tris-borate-EDTA buffer. .. The gels were run overnight at 15 V and then at 110 V for another 24 h. DNA was transferred to a Hybond N + membrane (GE Healthcare) and hybridized with 32 P-labeled TR probes to detect KSHV episome.

    Incubation:

    Article Title: Upregulation of heme oxygenase-1 protected against brain damage induced by transient cerebral ischemia-reperfusion injury in rats
    Article Snippet: .. Subsequently, brain tissue sections were dewaxed and rehydrated at 60°C for 2 h. These slides were incubated for 30 min at room temperature in a 20 µg/ml DNase-free proteinase K and rat terminal deoxynucleotidyl-transferase (both Sigma-Aldrich; Merck KGaA) reaction mix. .. Following rinsing with PBS 3 times for 15 min each time, these sections were treated with streptavidin horseradish peroxidase (1:200; Roche Diagnostics GmbH) at 37°C for 30 min and positive signals were enhanced with diaminobenzidine (1:200; Roche Diagnostics GmbH) at room temperature for 30 min.

    Activity Assay:

    Article Title: An Altered Pattern of Myocardial Histopathological and Molecular Changes Underlies the Different Characteristics of Type-1 and Type-2 Diabetic Cardiac Dysfunction
    Article Snippet: .. Rehydrated sections were treated with 20 μ g/mL DNase-free Proteinase-K (Sigma-Aldrich, Germany) to retrieve antigenic epitopes, followed by 3% hydrogen peroxide to quench endogenous peroxidase activity. .. Free 3′-OH termini were labeled with digoxigenin-dUTP for 1 h at 37°C utilizing a terminal deoxynucleotidyl transferase reaction mixture (Chemicon International, Temecula, CA, USA).

    Article Title: Differential expression of apoptosis-related factors induces the age-related apoptosis of the gracilis muscle in humans
    Article Snippet: .. The dehydrated sections were treated with 20 μg/ml DNase-free proteinase K (Sigma-Aldrich Corp., St. Louis, MO, USA) to retrieve antigenic epitopes, followed by 3% H2 O2 to quench endogenous peroxidase activity. .. Free 3′-OH termini were labeled with digoxigenin-dUTP for 1 h at 37ºC utilizing a terminal deoxynucleotidyl transferase reaction mixture.

    Microscopy:

    Article Title: Upregulation of heme oxygenase-1 protected against brain damage induced by transient cerebral ischemia-reperfusion injury in rats
    Article Snippet: Subsequently, brain tissue sections were dewaxed and rehydrated at 60°C for 2 h. These slides were incubated for 30 min at room temperature in a 20 µg/ml DNase-free proteinase K and rat terminal deoxynucleotidyl-transferase (both Sigma-Aldrich; Merck KGaA) reaction mix. .. The tissue slide was digitally photographed using an Olympus BX51 fluorescence microscope (magnification, ×400).

    DNA Laddering:

    Article Title: Characterization of protein marker expression, tumorigenicity, and doxorubicin chemoresistance in two new canine mammary tumor cell lines
    Article Snippet: .. DNA ladder assay After DOX (1000 nM) treatment, cells were collected and lysed at 55°C for 1 h in 500 μL lysis buffer that contained 100 mM NaCl, 10 mM Tris (pH 8.0), 25 mM EDTA, 0.5% SDS, 200 μg/mL DNase-free proteinase K (Sigma), and 50 μg/mL DNase-free RNase (Sigma). ..

    End Labeling:

    Article Title: Oral treatment with a zinc complex of acetylsalicylic acid prevents diabetic cardiomyopathy in a rat model of type-2 diabetes: activation of the Akt pathway
    Article Snippet: Paragraph title: Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) ... Briefly, the rehydrated sections were digested with 20 μg/ml DNase-free Proteinase K (Sigma-Aldrich, Germany) to retrieve antigenic epitopes, and endogenous peroxidases were blocked with 3 % hydrogen peroxide (H2 O2 ).

    Article Title: Differential expression of apoptosis-related factors induces the age-related apoptosis of the gracilis muscle in humans
    Article Snippet: Paragraph title: In situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) ... The dehydrated sections were treated with 20 μg/ml DNase-free proteinase K (Sigma-Aldrich Corp., St. Louis, MO, USA) to retrieve antigenic epitopes, followed by 3% H2 O2 to quench endogenous peroxidase activity.

    Lysis:

    Article Title: Minichromosome Maintenance Proteins Cooperate with LANA during the G1/S Phase of the Cell Cycle To Support Viral DNA Replication
    Article Snippet: .. KSHV-positive BCBL-1 and BC3 cells depleted of MCM3 and MCM6 were loaded onto the agarose gel with a lysis plug containing DNase-free proteinase K (Sigma-Aldrich, St. Louis, MO, USA) and SDS, followed by electrophoresis in a Tris-borate-EDTA buffer. .. The plugs were loaded on 0.8% Gardella gel and resolved at 108 V for 30 h. The gel was transferred onto a Hybond N+ membrane (GE Healthcare) and hybridized with 32 P-labeled TR probes to detect KSHV episome.

    Article Title: KSHV encoded LANA recruits Nucleosome Assembly Protein NAP1L1 for regulating viral DNA replication and transcription
    Article Snippet: .. KSHV-positive BCBL-1 and BC-3 cells depleted for LANA and NAP1L1 were loaded into the agarose gel with a lysis plug containing DNase-free Proteinase K (Sigma-Aldrich, St. Louis, MO, USA) and SDS followed by electrophoresis in a Tris-borate-EDTA buffer. .. The gels were run overnight at 15 V and then at 110 V for another 24 h. DNA was transferred to a Hybond N + membrane (GE Healthcare) and hybridized with 32 P-labeled TR probes to detect KSHV episome.

    Article Title: Characterization of protein marker expression, tumorigenicity, and doxorubicin chemoresistance in two new canine mammary tumor cell lines
    Article Snippet: .. DNA ladder assay After DOX (1000 nM) treatment, cells were collected and lysed at 55°C for 1 h in 500 μL lysis buffer that contained 100 mM NaCl, 10 mM Tris (pH 8.0), 25 mM EDTA, 0.5% SDS, 200 μg/mL DNase-free proteinase K (Sigma), and 50 μg/mL DNase-free RNase (Sigma). ..

    Article Title: GREEN TEA POLYPHENOLS MEDIATED APOPTOSIS IN INTESTINAL EPITHELIAL CELLS BY A FADD-DEPENDENT PATHWAY
    Article Snippet: .. Cells were collected and lysed with 1.0ml of lysis buffer [100 mm NaCl, 10 mm Tris (pH 8.0), 25mm ethylenediaminetetraacetic acid, 0.5% sodium dodecyl sulphate (SDS), 200 µg/ml of DNase-free proteinase K (Sigma)] and treated with DNAse free RNase (40 µg/ml) in the presence of 0.1% SDS for 1h at 37 °C. ..

    Real-time Polymerase Chain Reaction:

    Article Title: KSHV encoded LANA recruits Nucleosome Assembly Protein NAP1L1 for regulating viral DNA replication and transcription
    Article Snippet: KSHV-positive BCBL-1 and BC-3 cells depleted for LANA and NAP1L1 were loaded into the agarose gel with a lysis plug containing DNase-free Proteinase K (Sigma-Aldrich, St. Louis, MO, USA) and SDS followed by electrophoresis in a Tris-borate-EDTA buffer. .. Relative KSHV genome copies were analyzed by real-time PCR using a KSHV TR-specific primer.

    Staining:

    Article Title: Characterization of protein marker expression, tumorigenicity, and doxorubicin chemoresistance in two new canine mammary tumor cell lines
    Article Snippet: DNA ladder assay After DOX (1000 nM) treatment, cells were collected and lysed at 55°C for 1 h in 500 μL lysis buffer that contained 100 mM NaCl, 10 mM Tris (pH 8.0), 25 mM EDTA, 0.5% SDS, 200 μg/mL DNase-free proteinase K (Sigma), and 50 μg/mL DNase-free RNase (Sigma). .. DNA samples were then separated on l.6% agarose gels and stained with ethidium bromide.

    Article Title: GREEN TEA POLYPHENOLS MEDIATED APOPTOSIS IN INTESTINAL EPITHELIAL CELLS BY A FADD-DEPENDENT PATHWAY
    Article Snippet: Cells were collected and lysed with 1.0ml of lysis buffer [100 mm NaCl, 10 mm Tris (pH 8.0), 25mm ethylenediaminetetraacetic acid, 0.5% sodium dodecyl sulphate (SDS), 200 µg/ml of DNase-free proteinase K (Sigma)] and treated with DNAse free RNase (40 µg/ml) in the presence of 0.1% SDS for 1h at 37 °C. .. Gels were stained with ethidium bromide and visualized by ultraviolet fluorescence and photographed with a Polaroid camera system.

    Article Title: Upregulation of heme oxygenase-1 protected against brain damage induced by transient cerebral ischemia-reperfusion injury in rats
    Article Snippet: Paragraph title: Terminal deoxynucleotidyl-transferase-mediated dUTP nick end (TUNEL) staining ... Subsequently, brain tissue sections were dewaxed and rehydrated at 60°C for 2 h. These slides were incubated for 30 min at room temperature in a 20 µg/ml DNase-free proteinase K and rat terminal deoxynucleotidyl-transferase (both Sigma-Aldrich; Merck KGaA) reaction mix.

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  • Team
  • Advisors
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  • 99
    Millipore dnase proteinase k
    Dnase Proteinase K, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase proteinase k/product/Millipore
    Average 99 stars, based on 612 article reviews
    Price from $9.99 to $1999.99
    dnase proteinase k - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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