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TaKaRa dnapol
Dnapol, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dnapol/product/TaKaRa
Average 94 stars, based on 2 article reviews
Price from $9.99 to $1999.99
dnapol - by Bioz Stars, 2020-04
94/100 stars

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Clone Assay:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Article Title: Survivin regulates chromosome segregation by modulating the phosphorylation of Aurora B during porcine oocyte meiosis
Article Snippet: .. Survivin and CCNB1 genes were cloned with DNA polymerase (Takara, Prime STAR Mix, DP214-02). .. The products were used for A-tailing and TA cloning with DNA A-Tailing kit (Takara, 6109), then transformed into competent Escherichia coli cells.

Article Title: High-level expression of Thermomyces dupontii thermo-alkaline lipase in Pichia pastoris under the control of different promoters
Article Snippet: .. DNA polymerase (PrimeSTARTMHS ), restriction enzymes (EcoRI, NotI and SacI), in-fusion cloning kit and T4 -DNA ligase were purchased from Takara Biotechnology (Dalian, China). .. The gene of TDL (GenBank: ) without signal sequence was optimized according to the preference of P. pastoris and synthesized by the Genewiz (Suzhou, China).

Article Title: Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
Article Snippet: The fragment was cloned into pcDNA3.1 (+) vector to generate the plasmid pS. .. Pyrobest DNA polymerase (Takara, Japan) was used in all PCRs.

Amplification:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: The target genes were amplified from the genomic DNA of strain PmCQ2. .. DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in .

Article Title: Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
Article Snippet: The SARS-CoV S gene was amplified by PCR using the following primers: 5 0 -CGGGATCCA ACGAACATGTTTATTTTCTTATTATTTC-3 0 and 5 0 -CGGAATT CGTTTATGTGTAATGTAATTTGACACCC-3 0 . .. Pyrobest DNA polymerase (Takara, Japan) was used in all PCRs.

Article Title: The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1″-pase catalytic-site, critical for viral RNA replication.
Article Snippet: The polymerase chainreaction (PCR) was carried out in a 50 μl reaction volume, using 10 ng of replicon DNA, appropriate amounts of primers, dNTP mix, DNA polymerase and polymerase buffer under thermal conditions as per the manufacturer's manual (TaKaRa Bio Inc., Japan). .. Further, each amplicon was digested with Dpn I (Invitrogen, USA) in a 10 μl reaction volume at 37°C for 1.5 h. The digested mix (5 μl each) was transformed into DH5α XL-blue competent cells (Strata gene, USA) by the heat-shock method and plated on ampicillin-containing agar plates.

Synthesized:

Article Title: High-level expression of Thermomyces dupontii thermo-alkaline lipase in Pichia pastoris under the control of different promoters
Article Snippet: DNA polymerase (PrimeSTARTMHS ), restriction enzymes (EcoRI, NotI and SacI), in-fusion cloning kit and T4 -DNA ligase were purchased from Takara Biotechnology (Dalian, China). .. The gene of TDL (GenBank: ) without signal sequence was optimized according to the preference of P. pastoris and synthesized by the Genewiz (Suzhou, China).

Article Title: Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
Article Snippet: In order to create a codon optimized S gene, 60 overlapping primers were synthesized and assembled by overlapping PCR. .. Pyrobest DNA polymerase (Takara, Japan) was used in all PCRs.

TA Cloning:

Article Title: Survivin regulates chromosome segregation by modulating the phosphorylation of Aurora B during porcine oocyte meiosis
Article Snippet: Survivin and CCNB1 genes were cloned with DNA polymerase (Takara, Prime STAR Mix, DP214-02). .. The products were used for A-tailing and TA cloning with DNA A-Tailing kit (Takara, 6109), then transformed into competent Escherichia coli cells.

Construct:

Article Title: The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1″-pase catalytic-site, critical for viral RNA replication.
Article Snippet: Construction of X-domain mutant-replicons ORF1 X-domain a.a. mutants (pSK-GFP-Asn806Ala, pSK-GFP-Asn809Ala, pSK-GFP-His812Leu, pSK-GFP-Gly815Ala, pSK-GFP-Gly816Ala and pSK-GFP-Gly817Ala) were constructed in HEV1-SAR55 full-length (7.2 kb) genomic replicon (pSK-GFP) backbone (generous gift of Dr. Suzanne Emerson, National Institutes of Health, Bethesda, MD, USA) by site-directed mutagenesis as described previously (Parvez, 2013b) . .. The polymerase chainreaction (PCR) was carried out in a 50 μl reaction volume, using 10 ng of replicon DNA, appropriate amounts of primers, dNTP mix, DNA polymerase and polymerase buffer under thermal conditions as per the manufacturer's manual (TaKaRa Bio Inc., Japan).

Incubation:

Article Title: The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1″-pase catalytic-site, critical for viral RNA replication.
Article Snippet: The polymerase chainreaction (PCR) was carried out in a 50 μl reaction volume, using 10 ng of replicon DNA, appropriate amounts of primers, dNTP mix, DNA polymerase and polymerase buffer under thermal conditions as per the manufacturer's manual (TaKaRa Bio Inc., Japan). .. Following an overnight incubation at 37°C, bacterial colonies were picked and plasmids (Qiagen Plasmid Mini-prep Kit, Germany) were screened by restriction digestion.

Expressing:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: Paragraph title: Protein expression and purification ... DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in .

Article Title: High-level expression of Thermomyces dupontii thermo-alkaline lipase in Pichia pastoris under the control of different promoters
Article Snippet: P. pastoris X33 was purchased from Invitrogen (Carlsbad, CA, USA) and used as host for the expression of TDL. .. DNA polymerase (PrimeSTARTMHS ), restriction enzymes (EcoRI, NotI and SacI), in-fusion cloning kit and T4 -DNA ligase were purchased from Takara Biotechnology (Dalian, China).

Modification:

Article Title: Molecular mechanism of the synergistic activity of ethambutol and isoniazid against Mycobacterium tuberculosis
Article Snippet: .. DNA polymerase, dNTPs, restriction enzymes, modification enzymes, T4 ligase, and all antibiotics were obtained from TaKaRa Biotech (Shiga, Japan). .. Ni2+ -NTA–agarose, sensor chip CM5 was purchased from Qiagen (Hilden, Germany).

Transformation Assay:

Article Title: Survivin regulates chromosome segregation by modulating the phosphorylation of Aurora B during porcine oocyte meiosis
Article Snippet: Survivin and CCNB1 genes were cloned with DNA polymerase (Takara, Prime STAR Mix, DP214-02). .. The products were used for A-tailing and TA cloning with DNA A-Tailing kit (Takara, 6109), then transformed into competent Escherichia coli cells.

Article Title: The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1″-pase catalytic-site, critical for viral RNA replication.
Article Snippet: The polymerase chainreaction (PCR) was carried out in a 50 μl reaction volume, using 10 ng of replicon DNA, appropriate amounts of primers, dNTP mix, DNA polymerase and polymerase buffer under thermal conditions as per the manufacturer's manual (TaKaRa Bio Inc., Japan). .. Further, each amplicon was digested with Dpn I (Invitrogen, USA) in a 10 μl reaction volume at 37°C for 1.5 h. The digested mix (5 μl each) was transformed into DH5α XL-blue competent cells (Strata gene, USA) by the heat-shock method and plated on ampicillin-containing agar plates.

Derivative Assay:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: PCR was conducted to amplify the target region with genomic DNA derived from the cells. .. DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR.

High Performance Liquid Chromatography:

Article Title: Conditional control of RNA-guided nucleic acid cleavage and gene editing
Article Snippet: Pyrobest™ DNA Polymerase and PrimeSTAR HS DNA Polymerase were purchased from TaKaRa Shuzo Co. Ltd. (Tokyo, Japan). .. The oligonucleotides at HPLC purity were obtained from TaKaRa company (Dalian, China).

Electroporation:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: HepG2 cells were seeded in a 24-well plate 24 h prior to transfection and transfected with the pGK1.1-U6-IGF-1RgRNA plasmid via the electroporation method using a Neon® Transfection system (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR.

Cell Culture:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Sequencing:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR. .. Amplicons were sent to the Beijing Genomics Institute (Beijing, China) for deep sequencing.

Article Title: High-level expression of Thermomyces dupontii thermo-alkaline lipase in Pichia pastoris under the control of different promoters
Article Snippet: DNA polymerase (PrimeSTARTMHS ), restriction enzymes (EcoRI, NotI and SacI), in-fusion cloning kit and T4 -DNA ligase were purchased from Takara Biotechnology (Dalian, China). .. The gene of TDL (GenBank: ) without signal sequence was optimized according to the preference of P. pastoris and synthesized by the Genewiz (Suzhou, China).

Article Title: Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
Article Snippet: Pyrobest DNA polymerase (Takara, Japan) was used in all PCRs. .. In the first forward primer, tissue plasminogen activator (Tpa) signal sequence (MDAMKRGLCCVLLLCGAVFVSA) was introduced to replace the original signal peptide of the S gene (MFIFLLFLTLTSG).

Recombinant:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

DNA Extraction:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: After cell cultures were 70% confluent, they were harvested and the genomic DNA was extracted using DNA Extraction kits (Genloci Biotechnologies, Inc.). .. DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR.

Mutagenesis:

Article Title: The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1″-pase catalytic-site, critical for viral RNA replication.
Article Snippet: Paragraph title: Construction of X-domain mutant-replicons ... The polymerase chainreaction (PCR) was carried out in a 50 μl reaction volume, using 10 ng of replicon DNA, appropriate amounts of primers, dNTP mix, DNA polymerase and polymerase buffer under thermal conditions as per the manufacturer's manual (TaKaRa Bio Inc., Japan).

Isolation:

Article Title: Survivin regulates chromosome segregation by modulating the phosphorylation of Aurora B during porcine oocyte meiosis
Article Snippet: The cDNA of porcine oocytes, as the templates of S urvivin and CCNB1 genes cloning, were obtained with the method of RNA isolation and reverse transcription as previously mentioned. .. Survivin and CCNB1 genes were cloned with DNA polymerase (Takara, Prime STAR Mix, DP214-02).

Size-exclusion Chromatography:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR. .. PCR was performed using the following thermocycling conditions: 95°C for 10 sec, 60°C for 10 sec, 72°C for 20 sec for 30 cycles.

Purification:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: Paragraph title: Protein expression and purification ... DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in .

Polymerase Chain Reaction:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: .. DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR. ..

Article Title: Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
Article Snippet: In order to create a codon optimized S gene, 60 overlapping primers were synthesized and assembled by overlapping PCR. .. Pyrobest DNA polymerase (Takara, Japan) was used in all PCRs.

Article Title: The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1″-pase catalytic-site, critical for viral RNA replication.
Article Snippet: .. The polymerase chainreaction (PCR) was carried out in a 50 μl reaction volume, using 10 ng of replicon DNA, appropriate amounts of primers, dNTP mix, DNA polymerase and polymerase buffer under thermal conditions as per the manufacturer's manual (TaKaRa Bio Inc., Japan). .. The amplicons (5.0 μl each) were verified by agarose gel electrophoresis to confirm the correct size of the plasmid.

Affinity Chromatography:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Chromatin Immunoprecipitation:

Article Title: Molecular mechanism of the synergistic activity of ethambutol and isoniazid against Mycobacterium tuberculosis
Article Snippet: DNA polymerase, dNTPs, restriction enzymes, modification enzymes, T4 ligase, and all antibiotics were obtained from TaKaRa Biotech (Shiga, Japan). .. Ni2+ -NTA–agarose, sensor chip CM5 was purchased from Qiagen (Hilden, Germany).

Plasmid Preparation:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: HepG2 cells were seeded in a 24-well plate 24 h prior to transfection and transfected with the pGK1.1-U6-IGF-1RgRNA plasmid via the electroporation method using a Neon® Transfection system (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR.

Article Title: Survivin regulates chromosome segregation by modulating the phosphorylation of Aurora B during porcine oocyte meiosis
Article Snippet: Paragraph title: Vector construction and transcription in vitro ... Survivin and CCNB1 genes were cloned with DNA polymerase (Takara, Prime STAR Mix, DP214-02).

Article Title: High-level expression of Thermomyces dupontii thermo-alkaline lipase in Pichia pastoris under the control of different promoters
Article Snippet: The vector pPICZαA was also purchased from Invitrogen. .. DNA polymerase (PrimeSTARTMHS ), restriction enzymes (EcoRI, NotI and SacI), in-fusion cloning kit and T4 -DNA ligase were purchased from Takara Biotechnology (Dalian, China).

Article Title: Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
Article Snippet: The fragment was cloned into pcDNA3.1 (+) vector to generate the plasmid pS. .. Pyrobest DNA polymerase (Takara, Japan) was used in all PCRs.

Article Title: The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1″-pase catalytic-site, critical for viral RNA replication.
Article Snippet: The polymerase chainreaction (PCR) was carried out in a 50 μl reaction volume, using 10 ng of replicon DNA, appropriate amounts of primers, dNTP mix, DNA polymerase and polymerase buffer under thermal conditions as per the manufacturer's manual (TaKaRa Bio Inc., Japan). .. The amplicons (5.0 μl each) were verified by agarose gel electrophoresis to confirm the correct size of the plasmid.

Positron Emission Tomography:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Agarose Gel Electrophoresis:

Article Title: The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1″-pase catalytic-site, critical for viral RNA replication.
Article Snippet: The polymerase chainreaction (PCR) was carried out in a 50 μl reaction volume, using 10 ng of replicon DNA, appropriate amounts of primers, dNTP mix, DNA polymerase and polymerase buffer under thermal conditions as per the manufacturer's manual (TaKaRa Bio Inc., Japan). .. The amplicons (5.0 μl each) were verified by agarose gel electrophoresis to confirm the correct size of the plasmid.

In Vitro:

Article Title: Survivin regulates chromosome segregation by modulating the phosphorylation of Aurora B during porcine oocyte meiosis
Article Snippet: Paragraph title: Vector construction and transcription in vitro ... Survivin and CCNB1 genes were cloned with DNA polymerase (Takara, Prime STAR Mix, DP214-02).

Knock-Out:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: Paragraph title: Preparation of IGF-1R knockout cells ... DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR.

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  • 99
    TaKaRa taq dna polymerase
    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic <t>DNA.</t> PCR was performed on the isolated genomic DNA using <t>taq</t> DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for
    Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/TaKaRa
    Average 99 stars, based on 1599 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    TaKaRa thermostable dna polymerases
    Relative yield of the modified DNAs generated by PCR using various triphosphate analogs together with ( A ) <t>Taq</t> <t>DNA</t> polymerase, ( B ) Tth DNA polymerase, ( C ) Vent(exo-) DNA polymerase, ( D ) KOD Dash DNA polymerase and ( E ) KOD(exo-) DNA polymerase. The x -axis indicates the kind of triphosphate analog used, and the y -axis indicates the relative yield of the PCR product. The black and white bars indicate the relative yield of the PCR product generated from amplifying regions I and II, respectively. The relative standard deviations were less than ±6% for all reactions.
    Thermostable Dna Polymerases, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermostable dna polymerases/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thermostable dna polymerases - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Journal: Neuroscience letters

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain

    doi: 10.1016/j.neulet.2010.12.060

    Figure Lengend Snippet: (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Article Snippet: PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′).

    Techniques: Mutagenesis, Mouse Assay, Polymerase Chain Reaction, Isolation

    Blocking efficiency of three kinds of blocked primers in PCR amplifications mediated by Taq DNA polymerase and high-fidelity DNA polymerase. (a) Primer and template sets. WT and Mu indicate wild-type and mutant types, respectively. The 3'-blocked forward primer completely matches the WT sequence but forms a mismatch with the Mu sequence (Mu*1) at the 3'-end. The reverse primer matches both the WT and Mu sequences. The 3'-terminal nucleotide of the blocked primer is blocked with-Pi or-Amino C6, or replaced with ddCTP. (b) Blocking efficiency of-Pi,-Amino C6 or-ddC in PCR mediated by Taq DNA polymerase. (c) Blocking efficiency of-Pi,-Amino C6 or-ddC in PCR mediated by high-fidelity DNA polymerase (KOD FX). (d) Discrimination efficiency of the typical proofreading-PCR medicated by various amounts (0.5, 1, 1.5 and 2 U) of KOD FX DNA polymerase using the ddC-blocked primer. All PCR amplifications were performed in a total volume of 20 μL containing 1 U of Taq or KOD FX DNA polymerase (Panel b and c) or various amounts of KOD FX DNA polymerase (Panel d). The cycling conditions consisted of pre-denaturation at 94°C for 2 min, followed by 30 cycles of denaturation at 94°C for 20 s, annealing at 56°C for 20 s and extension at 72°C for 25 s. NTC: no-template control.

    Journal: PLoS ONE

    Article Title: Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer

    doi: 10.1371/journal.pone.0123468

    Figure Lengend Snippet: Blocking efficiency of three kinds of blocked primers in PCR amplifications mediated by Taq DNA polymerase and high-fidelity DNA polymerase. (a) Primer and template sets. WT and Mu indicate wild-type and mutant types, respectively. The 3'-blocked forward primer completely matches the WT sequence but forms a mismatch with the Mu sequence (Mu*1) at the 3'-end. The reverse primer matches both the WT and Mu sequences. The 3'-terminal nucleotide of the blocked primer is blocked with-Pi or-Amino C6, or replaced with ddCTP. (b) Blocking efficiency of-Pi,-Amino C6 or-ddC in PCR mediated by Taq DNA polymerase. (c) Blocking efficiency of-Pi,-Amino C6 or-ddC in PCR mediated by high-fidelity DNA polymerase (KOD FX). (d) Discrimination efficiency of the typical proofreading-PCR medicated by various amounts (0.5, 1, 1.5 and 2 U) of KOD FX DNA polymerase using the ddC-blocked primer. All PCR amplifications were performed in a total volume of 20 μL containing 1 U of Taq or KOD FX DNA polymerase (Panel b and c) or various amounts of KOD FX DNA polymerase (Panel d). The cycling conditions consisted of pre-denaturation at 94°C for 2 min, followed by 30 cycles of denaturation at 94°C for 20 s, annealing at 56°C for 20 s and extension at 72°C for 25 s. NTC: no-template control.

    Article Snippet: The results showed that the use of 0.1–0.15 U of high-fidelity DNA polymerase combined with 1 U of Taq DNA polymerase in per 20 μL reaction resulted in the best discrimination and amplification effects ( ).

    Techniques: Blocking Assay, Polymerase Chain Reaction, Mutagenesis, Sequencing

    Optimization of annealing temperature and amount of high-fidelity DNA polymerase in the modified PR-PCR. (a) Discrimination efficiency of the modified PR-PCR for known mutations using a mixture of 1 U of Taq DNA polymerase and various amount (0.05, 0.1 and 0.3 U) of KOD FX DNA polymerase in the reaction. The PCR amplifications were performed in a total volume of 20 μL under cycling conditions consisting of pre-denaturation at 94°C for 2 min, followed by 30 cycles of denaturation at 94°C for 20 s, annealing at 56°C for 20 s and extension at 72°C for 25 s. (b) Optimization of annealing temperature for the modified PR-PCR using a mixture of 1 U of Taq DNA polymerase and 0.1 U of KOD FX DNA polymerase. As the optimal discrimination efficiency was obtained when the annealing temperature was within a scope of 61.8°C–62.6°C, the results from 50°C to 61.2°C were not shown. The temperature 62.2°C was selected for subsequent experiments. (c) Optimization of amount of high-fidelity DNA polymerase for the modified PR-PCR with an input of 1 U of Taq DNA polymerase at an annealing temperature of 62.2°C.

    Journal: PLoS ONE

    Article Title: Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer

    doi: 10.1371/journal.pone.0123468

    Figure Lengend Snippet: Optimization of annealing temperature and amount of high-fidelity DNA polymerase in the modified PR-PCR. (a) Discrimination efficiency of the modified PR-PCR for known mutations using a mixture of 1 U of Taq DNA polymerase and various amount (0.05, 0.1 and 0.3 U) of KOD FX DNA polymerase in the reaction. The PCR amplifications were performed in a total volume of 20 μL under cycling conditions consisting of pre-denaturation at 94°C for 2 min, followed by 30 cycles of denaturation at 94°C for 20 s, annealing at 56°C for 20 s and extension at 72°C for 25 s. (b) Optimization of annealing temperature for the modified PR-PCR using a mixture of 1 U of Taq DNA polymerase and 0.1 U of KOD FX DNA polymerase. As the optimal discrimination efficiency was obtained when the annealing temperature was within a scope of 61.8°C–62.6°C, the results from 50°C to 61.2°C were not shown. The temperature 62.2°C was selected for subsequent experiments. (c) Optimization of amount of high-fidelity DNA polymerase for the modified PR-PCR with an input of 1 U of Taq DNA polymerase at an annealing temperature of 62.2°C.

    Article Snippet: The results showed that the use of 0.1–0.15 U of high-fidelity DNA polymerase combined with 1 U of Taq DNA polymerase in per 20 μL reaction resulted in the best discrimination and amplification effects ( ).

    Techniques: Modification, Polymerase Chain Reaction

    Sensitivity and selectivity of the modified PR-PCR for mutation detection using a fusion-blocked primer and adaptor. (a) Diagram of the fusion-blocked primer and adaptor used in the reactions. (b) Primer and adaptor sequences. (c) Sensitivity of the modified PR-PCR. (d) Selectivity of the modified PR-PCR. All reactions were performed in a total volume of 25 μL containing a mixture of 1 μL of gDNA template at 50 ng/μL, 2.5 μL of (10×) Taq PCR buffer, 0.2 mM dNTPs, 0.15 U of PrimeSTAR HS DNA polymerase, 0.75 U of Taq DNA polymerase, 1 μL of DMSO and 0.3 μM each of the reverse primer, fusion-blocked forward primer and adaptor. The DNA templates were prepared by mixing different amounts of MCF-7 (WT) gDNA (from 0 to 50 ng) among HCC1937 (mutant type, MT) gDNA (from 50 to 0 ng) with concentrations from 0 to 100%. The reactions were performed under cycling conditions consisting of pre-denaturation at 94°C for 2 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 59°C for 30 s and extension at 72°C for 20 s.

    Journal: PLoS ONE

    Article Title: Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer

    doi: 10.1371/journal.pone.0123468

    Figure Lengend Snippet: Sensitivity and selectivity of the modified PR-PCR for mutation detection using a fusion-blocked primer and adaptor. (a) Diagram of the fusion-blocked primer and adaptor used in the reactions. (b) Primer and adaptor sequences. (c) Sensitivity of the modified PR-PCR. (d) Selectivity of the modified PR-PCR. All reactions were performed in a total volume of 25 μL containing a mixture of 1 μL of gDNA template at 50 ng/μL, 2.5 μL of (10×) Taq PCR buffer, 0.2 mM dNTPs, 0.15 U of PrimeSTAR HS DNA polymerase, 0.75 U of Taq DNA polymerase, 1 μL of DMSO and 0.3 μM each of the reverse primer, fusion-blocked forward primer and adaptor. The DNA templates were prepared by mixing different amounts of MCF-7 (WT) gDNA (from 0 to 50 ng) among HCC1937 (mutant type, MT) gDNA (from 50 to 0 ng) with concentrations from 0 to 100%. The reactions were performed under cycling conditions consisting of pre-denaturation at 94°C for 2 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 59°C for 30 s and extension at 72°C for 20 s.

    Article Snippet: The results showed that the use of 0.1–0.15 U of high-fidelity DNA polymerase combined with 1 U of Taq DNA polymerase in per 20 μL reaction resulted in the best discrimination and amplification effects ( ).

    Techniques: Modification, Polymerase Chain Reaction, Mutagenesis

    Screening of DNA polymerases for single-nucleotide insertion. ( A ) The reaction for ImO N :NaN O base pair. ( B ) The reaction for ImN O :NaO N base pair. All reactions used the primer-template combination 5′-FITC-GTTCTGGATGGTCAGCGCAC-3′ (20mer) and 3′-CAAGACCTACCAGTCGCGTG X GAACGGGTG-5′ (30mer, X = ImO N , NaN O , ImN O or NaO N ). Lanes 1 and 14 indicate the 20-mer primer; lanes 2, 8, 15 and 21 indicate the results of KF (exo − ); lanes 3, 9 16, and 22 indicate those of Taq DNA polymerase; lanes 4, 10, 17 and 23 indicate those of Tth DNA polymerase; lanes 5, 11, 18 and 24 indicate those of Vent (exo − ) DNA polymerase; lanes 6, 12, 19 and 25 indicate those of Deep Vent (exo − ) DNA polymerase; and lanes 7, 13, 20 and 26 indicate those of KOD Dash DNA polymerase.

    Journal: Nucleic Acids Research

    Article Title: Unnatural imidazopyridopyrimidine:naphthyridine base pairs: selective incorporation and extension reaction by Deep Vent (exo− ) DNA polymerase

    doi: 10.1093/nar/gkp611

    Figure Lengend Snippet: Screening of DNA polymerases for single-nucleotide insertion. ( A ) The reaction for ImO N :NaN O base pair. ( B ) The reaction for ImN O :NaO N base pair. All reactions used the primer-template combination 5′-FITC-GTTCTGGATGGTCAGCGCAC-3′ (20mer) and 3′-CAAGACCTACCAGTCGCGTG X GAACGGGTG-5′ (30mer, X = ImO N , NaN O , ImN O or NaO N ). Lanes 1 and 14 indicate the 20-mer primer; lanes 2, 8, 15 and 21 indicate the results of KF (exo − ); lanes 3, 9 16, and 22 indicate those of Taq DNA polymerase; lanes 4, 10, 17 and 23 indicate those of Tth DNA polymerase; lanes 5, 11, 18 and 24 indicate those of Vent (exo − ) DNA polymerase; lanes 6, 12, 19 and 25 indicate those of Deep Vent (exo − ) DNA polymerase; and lanes 7, 13, 20 and 26 indicate those of KOD Dash DNA polymerase.

    Article Snippet: Taq DNA polymerase was purchased from TaKaRa.

    Techniques:

    Relative yield of the modified DNAs generated by PCR using various triphosphate analogs together with ( A ) Taq DNA polymerase, ( B ) Tth DNA polymerase, ( C ) Vent(exo-) DNA polymerase, ( D ) KOD Dash DNA polymerase and ( E ) KOD(exo-) DNA polymerase. The x -axis indicates the kind of triphosphate analog used, and the y -axis indicates the relative yield of the PCR product. The black and white bars indicate the relative yield of the PCR product generated from amplifying regions I and II, respectively. The relative standard deviations were less than ±6% for all reactions.

    Journal: Nucleic Acids Research

    Article Title: Systematic characterization of 2?-deoxynucleoside- 5?-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA

    doi: 10.1093/nar/gkl637

    Figure Lengend Snippet: Relative yield of the modified DNAs generated by PCR using various triphosphate analogs together with ( A ) Taq DNA polymerase, ( B ) Tth DNA polymerase, ( C ) Vent(exo-) DNA polymerase, ( D ) KOD Dash DNA polymerase and ( E ) KOD(exo-) DNA polymerase. The x -axis indicates the kind of triphosphate analog used, and the y -axis indicates the relative yield of the PCR product. The black and white bars indicate the relative yield of the PCR product generated from amplifying regions I and II, respectively. The relative standard deviations were less than ±6% for all reactions.

    Article Snippet: Materials The following commercial available thermostable DNA polymerases were purchased: Taq (Takara Bio), Tth (Toyobo), Vent(exo-) (New England Biolabs) and KOD Dash (Toyobo).

    Techniques: Modification, Generated, Polymerase Chain Reaction

    Representative ultraviolet images from ethidium bromide-stained PAGE gels of the 110 nt PCR product derived from pUC18 (amplifying region I) and the C5-modified nucleoside triphosphates. ( A–C ) The reaction mixtures containing dATP, dGTP, dCTP and T AL (lane 3), T AC (lane 4), T AF (lane 5), T PA (lane 6), T PN (lane 7), T PR (lane 8), T A2 (lane 11), T A4 (lane 12), T A6 (lane 13), T G6 (lane 14), T ME (lane 15), T CN (lane 16), or T DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures do not contain natural TTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and dCTP; lanes 1 and 9). ( D–F ) Reaction mixtures containing dATP, dGTP, TTP and C AL (lane 3), C AC (lane 4), C AF (lane 5), C PA (lane 6), C PN (lane 7), C PR (lane 8), C A2 (lane 11), C A4 (lane 12), C A6 (lane 13), C G6 (lane 14), C ME (lane 15), C CN (lane 16) or C DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures did not contain natural dCTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and TTP; lanes 1 and 9). The thermostable DNA polymerases used were Taq (A, D), Vent(exo-) (B and E), and KOD Dash (C and F).

    Journal: Nucleic Acids Research

    Article Title: Systematic characterization of 2?-deoxynucleoside- 5?-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA

    doi: 10.1093/nar/gkl637

    Figure Lengend Snippet: Representative ultraviolet images from ethidium bromide-stained PAGE gels of the 110 nt PCR product derived from pUC18 (amplifying region I) and the C5-modified nucleoside triphosphates. ( A–C ) The reaction mixtures containing dATP, dGTP, dCTP and T AL (lane 3), T AC (lane 4), T AF (lane 5), T PA (lane 6), T PN (lane 7), T PR (lane 8), T A2 (lane 11), T A4 (lane 12), T A6 (lane 13), T G6 (lane 14), T ME (lane 15), T CN (lane 16), or T DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures do not contain natural TTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and dCTP; lanes 1 and 9). ( D–F ) Reaction mixtures containing dATP, dGTP, TTP and C AL (lane 3), C AC (lane 4), C AF (lane 5), C PA (lane 6), C PN (lane 7), C PR (lane 8), C A2 (lane 11), C A4 (lane 12), C A6 (lane 13), C G6 (lane 14), C ME (lane 15), C CN (lane 16) or C DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures did not contain natural dCTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and TTP; lanes 1 and 9). The thermostable DNA polymerases used were Taq (A, D), Vent(exo-) (B and E), and KOD Dash (C and F).

    Article Snippet: Materials The following commercial available thermostable DNA polymerases were purchased: Taq (Takara Bio), Tth (Toyobo), Vent(exo-) (New England Biolabs) and KOD Dash (Toyobo).

    Techniques: Staining, Polyacrylamide Gel Electrophoresis, Polymerase Chain Reaction, Derivative Assay, Modification, Electrophoresis, Positive Control, Generated, Negative Control