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US Biological Life Sciences dnaase i
Dnaase I, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dnaase i/product/US Biological Life Sciences
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dnaase i - by Bioz Stars, 2020-04
85/100 stars

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High Performance Liquid Chromatography:

Article Title: Role of human CYP1A1 and NAT2 in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mutagenicity and DNA adducts
Article Snippet: DNA samples (200 μg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-PhIP-D3) were digested at 37°C with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight. .. Samples were subjected to binary gradient HPLC and introduced into a Micromass Quattro LC triple quadrupole mass spectrometer using a custom-built nanospray as described previously ( ; ).

Article Title: Effect of Rapid Human N-acetyltransferase 2 Haplotype on DNA Damage and Mutagenesis Induced by 2-Amino-3-methylimidazo [4,5-f] quinoline (IQ) and 2-Amino-3,8-dimethylimidazo-[4,5-f]quinoxaline (MeIQx)
Article Snippet: DNA samples (200 μg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-MeIQx-d3) were digested at 37°C with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight. .. Samples were subjected to binary gradient HPLC and introduced into a Micromass Quattro LC triple quadrupole mass spectrometer using a custom-built nanospray as described previously [ , ].

Article Title: Differences between human slow N-acetyltransferase 2 alleles in levels of 4-aminobiphenyl-induced DNA adducts and mutations
Article Snippet: DNA samples (200 µg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-ABP-D5) were digested at 37ºC with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight. .. Samples were subjected to binary gradient high performance liquid chromatography (HPLC) and introduced into a Micromass Quattro LC triple quadrupole mass spectrometer using a custom-built nanospray as described previously [ , ].

DNA Extraction:

Article Title: NATb/NAT1*4 promotes greater arylamine N-acetyltransferase 1 mediated DNA adducts and mutations than NATa/NAT1*4 following exposure to 4-aminobiphenyl
Article Snippet: Paragraph title: DNA Isolation and dG-C8-ABP Quantitation ... Five hundred pg of internal standard (dG-C8-ABP-d5, Toronto Research Chemicals, North York, Ontario, Canada) was added to 30 μg of sample DNA, treated with 10 units DNase I (US Biological, Swampscott, MA) for 1 h at 37°C followed by treatment with 10 units nuclease P1 (Sigma) for 6 h. The reactions were then treated with 10 units of alkaline phosphatase (Sigma) overnight at 37°C.

Transfection:

Article Title: NATb/NAT1*4 promotes greater arylamine N-acetyltransferase 1 mediated DNA adducts and mutations than NATa/NAT1*4 following exposure to 4-aminobiphenyl
Article Snippet: Stably transfected cells grown to approximately 80% confluency in 15 cm dishes were incubated in complete α-MEM media containing 1.56, 3.13, 6.25, 12.5 μM ABP or vehicle alone (0.5% DMSO) at 37°C. .. Five hundred pg of internal standard (dG-C8-ABP-d5, Toronto Research Chemicals, North York, Ontario, Canada) was added to 30 μg of sample DNA, treated with 10 units DNase I (US Biological, Swampscott, MA) for 1 h at 37°C followed by treatment with 10 units nuclease P1 (Sigma) for 6 h. The reactions were then treated with 10 units of alkaline phosphatase (Sigma) overnight at 37°C.

Mass Spectrometry:

Article Title: Role of human CYP1A1 and NAT2 in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mutagenicity and DNA adducts
Article Snippet: DNA samples (200 μg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-PhIP-D3) were digested at 37°C with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight. .. Samples were subjected to binary gradient HPLC and introduced into a Micromass Quattro LC triple quadrupole mass spectrometer using a custom-built nanospray as described previously ( ; ).

Article Title: Effect of Rapid Human N-acetyltransferase 2 Haplotype on DNA Damage and Mutagenesis Induced by 2-Amino-3-methylimidazo [4,5-f] quinoline (IQ) and 2-Amino-3,8-dimethylimidazo-[4,5-f]quinoxaline (MeIQx)
Article Snippet: DNA samples (200 μg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-MeIQx-d3) were digested at 37°C with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight. .. Samples were subjected to binary gradient HPLC and introduced into a Micromass Quattro LC triple quadrupole mass spectrometer using a custom-built nanospray as described previously [ , ].

Article Title: NATb/NAT1*4 promotes greater arylamine N-acetyltransferase 1 mediated DNA adducts and mutations than NATa/NAT1*4 following exposure to 4-aminobiphenyl
Article Snippet: Five hundred pg of internal standard (dG-C8-ABP-d5, Toronto Research Chemicals, North York, Ontario, Canada) was added to 30 μg of sample DNA, treated with 10 units DNase I (US Biological, Swampscott, MA) for 1 h at 37°C followed by treatment with 10 units nuclease P1 (Sigma) for 6 h. The reactions were then treated with 10 units of alkaline phosphatase (Sigma) overnight at 37°C. .. The samples were reconstituted with 25 μL 5% ACN in 2.5 mM NH4 HCO3 just before analysis and 10 μL of the sample was analyzed by Accela LC System (Thermo Scientific, San Jose, CA) coupled with a LTQ-Orbitrap XL mass spectrometer (Thermo Scientific, San Jose, CA).

Article Title: Differences between human slow N-acetyltransferase 2 alleles in levels of 4-aminobiphenyl-induced DNA adducts and mutations
Article Snippet: DNA samples (200 µg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-ABP-D5) were digested at 37ºC with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight. .. Samples were subjected to binary gradient high performance liquid chromatography (HPLC) and introduced into a Micromass Quattro LC triple quadrupole mass spectrometer using a custom-built nanospray as described previously [ , ].

Stable Transfection:

Article Title: NATb/NAT1*4 promotes greater arylamine N-acetyltransferase 1 mediated DNA adducts and mutations than NATa/NAT1*4 following exposure to 4-aminobiphenyl
Article Snippet: Stably transfected cells grown to approximately 80% confluency in 15 cm dishes were incubated in complete α-MEM media containing 1.56, 3.13, 6.25, 12.5 μM ABP or vehicle alone (0.5% DMSO) at 37°C. .. Five hundred pg of internal standard (dG-C8-ABP-d5, Toronto Research Chemicals, North York, Ontario, Canada) was added to 30 μg of sample DNA, treated with 10 units DNase I (US Biological, Swampscott, MA) for 1 h at 37°C followed by treatment with 10 units nuclease P1 (Sigma) for 6 h. The reactions were then treated with 10 units of alkaline phosphatase (Sigma) overnight at 37°C.

Homogenization:

Article Title: NATb/NAT1*4 promotes greater arylamine N-acetyltransferase 1 mediated DNA adducts and mutations than NATa/NAT1*4 following exposure to 4-aminobiphenyl
Article Snippet: The cells were collected following 24 h of treatment, centrifuged for 5 min at 13,000xg, and the pellet was resuspended in 2 volumes of homogenization buffer (20 mM sodium phosphate pH 7.4, 1 mM EDTA), 0.1 volumes of 10% SDS and 0.1 volume of 20 mg/mL Proteinase K and allowed to incubate overnight at 37°C. .. Five hundred pg of internal standard (dG-C8-ABP-d5, Toronto Research Chemicals, North York, Ontario, Canada) was added to 30 μg of sample DNA, treated with 10 units DNase I (US Biological, Swampscott, MA) for 1 h at 37°C followed by treatment with 10 units nuclease P1 (Sigma) for 6 h. The reactions were then treated with 10 units of alkaline phosphatase (Sigma) overnight at 37°C.

Mutagenesis:

Article Title: Effect of Rapid Human N-acetyltransferase 2 Haplotype on DNA Damage and Mutagenesis Induced by 2-Amino-3-methylimidazo [4,5-f] quinoline (IQ) and 2-Amino-3,8-dimethylimidazo-[4,5-f]quinoxaline (MeIQx)
Article Snippet: Cells grown in 15-cm plates were treated separately with IQ or MeIQx as described above for the survival and mutagenesis assays. .. DNA samples (200 μg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-MeIQx-d3) were digested at 37°C with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight.

Isolation:

Article Title: Arterially Perfused Neurosphere-Derived Cells Distribute Outside the Ischemic Core in a Model of Transient Focal Ischemia and Reperfusion In Vitro
Article Snippet: Paragraph title: Neural Stem Cell preparation and perfusion in the isolated brain ... The tissue suspension was collected and treated with collagenase Type I (Gibco, Paisley Scotland, U.K.) and DNAase I (USBiological DBA Italia, Italy) for 30–45 minutes at 37°C in a humidified atmosphere containing 5% CO2 .

Transferring:

Article Title: Arterially Perfused Neurosphere-Derived Cells Distribute Outside the Ischemic Core in a Model of Transient Focal Ischemia and Reperfusion In Vitro
Article Snippet: The brain of each mouse was removed and mechanically triturated with a fire-polished Pasteur pipette. .. The tissue suspension was collected and treated with collagenase Type I (Gibco, Paisley Scotland, U.K.) and DNAase I (USBiological DBA Italia, Italy) for 30–45 minutes at 37°C in a humidified atmosphere containing 5% CO2 .

Quantitation Assay:

Article Title: Role of human CYP1A1 and NAT2 in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mutagenicity and DNA adducts
Article Snippet: Paragraph title: Identification and quantitation of PhIP- DNA adducts ... DNA samples (200 μg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-PhIP-D3) were digested at 37°C with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight.

Article Title: Effect of Rapid Human N-acetyltransferase 2 Haplotype on DNA Damage and Mutagenesis Induced by 2-Amino-3-methylimidazo [4,5-f] quinoline (IQ) and 2-Amino-3,8-dimethylimidazo-[4,5-f]quinoxaline (MeIQx)
Article Snippet: Paragraph title: 2.6 Identification and quantitation of IQ- and MeIQx-DNA adducts ... DNA samples (200 μg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-MeIQx-d3) were digested at 37°C with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight.

Article Title: NATb/NAT1*4 promotes greater arylamine N-acetyltransferase 1 mediated DNA adducts and mutations than NATa/NAT1*4 following exposure to 4-aminobiphenyl
Article Snippet: Paragraph title: DNA Isolation and dG-C8-ABP Quantitation ... Five hundred pg of internal standard (dG-C8-ABP-d5, Toronto Research Chemicals, North York, Ontario, Canada) was added to 30 μg of sample DNA, treated with 10 units DNase I (US Biological, Swampscott, MA) for 1 h at 37°C followed by treatment with 10 units nuclease P1 (Sigma) for 6 h. The reactions were then treated with 10 units of alkaline phosphatase (Sigma) overnight at 37°C.

Article Title: Differences between human slow N-acetyltransferase 2 alleles in levels of 4-aminobiphenyl-induced DNA adducts and mutations
Article Snippet: Paragraph title: 2.4. Identification and quantitation of ABP-DNA adducts ... DNA samples (200 µg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-ABP-D5) were digested at 37ºC with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight.

Spectrophotometry:

Article Title: NATb/NAT1*4 promotes greater arylamine N-acetyltransferase 1 mediated DNA adducts and mutations than NATa/NAT1*4 following exposure to 4-aminobiphenyl
Article Snippet: The DNA was quantitated by spectrophotometry at A260 . .. Five hundred pg of internal standard (dG-C8-ABP-d5, Toronto Research Chemicals, North York, Ontario, Canada) was added to 30 μg of sample DNA, treated with 10 units DNase I (US Biological, Swampscott, MA) for 1 h at 37°C followed by treatment with 10 units nuclease P1 (Sigma) for 6 h. The reactions were then treated with 10 units of alkaline phosphatase (Sigma) overnight at 37°C.

Mouse Assay:

Article Title: Arterially Perfused Neurosphere-Derived Cells Distribute Outside the Ischemic Core in a Model of Transient Focal Ischemia and Reperfusion In Vitro
Article Snippet: Neural Stem Cell preparation and perfusion in the isolated brain Neurosphere-derived cells (NC) were isolated from newborn mice C57BL/6 (Charles River, Calco, Italy). .. The tissue suspension was collected and treated with collagenase Type I (Gibco, Paisley Scotland, U.K.) and DNAase I (USBiological DBA Italia, Italy) for 30–45 minutes at 37°C in a humidified atmosphere containing 5% CO2 .

Incubation:

Article Title: NATb/NAT1*4 promotes greater arylamine N-acetyltransferase 1 mediated DNA adducts and mutations than NATa/NAT1*4 following exposure to 4-aminobiphenyl
Article Snippet: Stably transfected cells grown to approximately 80% confluency in 15 cm dishes were incubated in complete α-MEM media containing 1.56, 3.13, 6.25, 12.5 μM ABP or vehicle alone (0.5% DMSO) at 37°C. .. Five hundred pg of internal standard (dG-C8-ABP-d5, Toronto Research Chemicals, North York, Ontario, Canada) was added to 30 μg of sample DNA, treated with 10 units DNase I (US Biological, Swampscott, MA) for 1 h at 37°C followed by treatment with 10 units nuclease P1 (Sigma) for 6 h. The reactions were then treated with 10 units of alkaline phosphatase (Sigma) overnight at 37°C.

Cell Culture:

Article Title: Arterially Perfused Neurosphere-Derived Cells Distribute Outside the Ischemic Core in a Model of Transient Focal Ischemia and Reperfusion In Vitro
Article Snippet: The tissue suspension was collected and treated with collagenase Type I (Gibco, Paisley Scotland, U.K.) and DNAase I (USBiological DBA Italia, Italy) for 30–45 minutes at 37°C in a humidified atmosphere containing 5% CO2 . .. Pellets were dissociated in a 200 µl pipette tip, plated in an untreated 25 cm2 tissue culture flasks and cultured in medium optimized for NCs growth in neurospheres Neurospheres were harvested and dissociated at single cells with collagenase I and re-plated under the conditions described above.

Spectroscopy:

Article Title: Role of human CYP1A1 and NAT2 in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mutagenicity and DNA adducts
Article Snippet: DNA quality was monitored by UV spectroscopy using A260/280 nm and this ratio was consistently above 1.9. .. DNA samples (200 μg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-PhIP-D3) were digested at 37°C with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight.

Article Title: Effect of Rapid Human N-acetyltransferase 2 Haplotype on DNA Damage and Mutagenesis Induced by 2-Amino-3-methylimidazo [4,5-f] quinoline (IQ) and 2-Amino-3,8-dimethylimidazo-[4,5-f]quinoxaline (MeIQx)
Article Snippet: DNA quality was monitored by UV spectroscopy using A260/280 nm and this ratio was consistently above 1.9. .. DNA samples (200 μg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-MeIQx-d3) were digested at 37°C with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight.

Article Title: Differences between human slow N-acetyltransferase 2 alleles in levels of 4-aminobiphenyl-induced DNA adducts and mutations
Article Snippet: DNA quality was monitored by UV spectroscopy using A260/280 nm and this ratio was consistently above 1.9. .. DNA samples (200 µg) added to 1 ng (3.3 adducts per 106 DNA bases) deuterated internal standard (dG-C8-ABP-D5) were digested at 37ºC with 10 units DNAse I (US Biological) for 1 h followed by 5 units micrococcal nuclease (Sigma), 5 units nuclease P1 (US Biological), 0.01 units spleen phosphodiesterase (Sigma), and 0.01 units snake venom phosphodiesterase (Sigma) for 6 h followed by 5 units alkaline phosphatase (Sigma) overnight.

Centrifugation:

Article Title: NATb/NAT1*4 promotes greater arylamine N-acetyltransferase 1 mediated DNA adducts and mutations than NATa/NAT1*4 following exposure to 4-aminobiphenyl
Article Snippet: Five hundred pg of internal standard (dG-C8-ABP-d5, Toronto Research Chemicals, North York, Ontario, Canada) was added to 30 μg of sample DNA, treated with 10 units DNase I (US Biological, Swampscott, MA) for 1 h at 37°C followed by treatment with 10 units nuclease P1 (Sigma) for 6 h. The reactions were then treated with 10 units of alkaline phosphatase (Sigma) overnight at 37°C. .. The samples were then loaded onto PepClean C-18 Spin Columns (Thermo Fisher Scientific), washed with 10% acetonitrile (ACN), eluted with 50% ACN by centrifugation at 2000xg and dried.

Generated:

Article Title: Arterially Perfused Neurosphere-Derived Cells Distribute Outside the Ischemic Core in a Model of Transient Focal Ischemia and Reperfusion In Vitro
Article Snippet: The tissue suspension was collected and treated with collagenase Type I (Gibco, Paisley Scotland, U.K.) and DNAase I (USBiological DBA Italia, Italy) for 30–45 minutes at 37°C in a humidified atmosphere containing 5% CO2 . .. After performing this procedure twice in order to eliminate short-term dividing precursors, bulk cultures were generated by passing the cells at higher density in the same growth medium every 10 days.

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    US Biological Life Sciences dnase i
    Dnase I, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/US Biological Life Sciences
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    85
    US Biological Life Sciences dnase type i
    Dnase Type I, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase type i/product/US Biological Life Sciences
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase type i - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

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