Structured Review

ATG:biosynthetics dna sequence
Dna Sequence, supplied by ATG:biosynthetics, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna sequence/product/ATG:biosynthetics
Average 93 stars, based on 7 article reviews
Price from $9.99 to $1999.99
dna sequence - by Bioz Stars, 2020-09
93/100 stars

Images

Related Articles

Clone Assay:

Article Title: Defining HIV-1 Envelope N-Glycan Microdomains through Site-Specific Heterogeneity Profiles
Article Snippet: .. Both DNA sequences coding for respective gp160 protein were codon optimized, synthesized by a commercial supplier (ATG:biosynthetics, Merzhausen, Germany), and cloned in frame into expression plasmid pcDNA3.1. .. Both gp160 amino acid sequences are numbered according to the alignment with the HIV-1 HXB2 sequence (GenBank number ).

Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
Article Snippet: .. The synthetic genes were cloned into the expression vector pET17b, and the DNA sequences were determined by means of sequencing (ATG Biosynthetics). .. For more information on expression and purification of Der p 2/1 mosaic proteins, see the Methods section in this article’s Online Repository.

Article Title: Impact of glycan positioning on HIV-1 Env glycan shield density, function, and antibody recognition
Article Snippet: .. Both DNA sequences coding for the respective gp160 protein were codon-optimized, synthesized by commercial supplier (ATG:biosynthetics, Merzhausen, Germany), and cloned in frame into expression plasmid pcDNA3.1 (yielding pcDNA3.1-WEAU gp160 clones that were used for production of Env-pseudotyped HIV-1). .. Region corresponding to gp120 amino-acid sequences shown in are numbered according to the alignment with HIV-1HXB2 sequence (GenBank accession number K03455 ).

In Vitro:

Article Title: Catalytic surface radical in dye-decolorizing peroxidase: a computational, spectroscopic and site-directed mutagenesis study
Article Snippet: .. DyP production, activation and purification The DNA sequence coding mature DyP-I from A. auricula-judae (GenBank® accession number JQ650250) [ ] was synthesized (ATG:biosynthetics), expressed in E. coli , activated in vitro , and purified as described in [ ] (see the Supplementary Methods for details). .. Site-directed mutagenesis and chemical modification of DyP Simple DyP variants were produced by PCR using the pET23a-DyPI vector harbouring the mature protein-coding sequence of A. auricula-judae DyP as a template.

Synthesized:

Article Title: Defining HIV-1 Envelope N-Glycan Microdomains through Site-Specific Heterogeneity Profiles
Article Snippet: .. Both DNA sequences coding for respective gp160 protein were codon optimized, synthesized by a commercial supplier (ATG:biosynthetics, Merzhausen, Germany), and cloned in frame into expression plasmid pcDNA3.1. .. Both gp160 amino acid sequences are numbered according to the alignment with the HIV-1 HXB2 sequence (GenBank number ).

Article Title: Impact of glycan positioning on HIV-1 Env glycan shield density, function, and antibody recognition
Article Snippet: .. Both DNA sequences coding for the respective gp160 protein were codon-optimized, synthesized by commercial supplier (ATG:biosynthetics, Merzhausen, Germany), and cloned in frame into expression plasmid pcDNA3.1 (yielding pcDNA3.1-WEAU gp160 clones that were used for production of Env-pseudotyped HIV-1). .. Region corresponding to gp120 amino-acid sequences shown in are numbered according to the alignment with HIV-1HXB2 sequence (GenBank accession number K03455 ).

Article Title: Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date
Article Snippet: .. Then, the DNA sequences encoding the most probable amino-acid sequences for the four reconstructed nodes, together with the two alternative sequences from the CaPo subsets, were synthesized by ATG:biosynthetics (Merzhausen, Germany) after optimizing the codon usage for high expression in E. coli using OPTIMIZER [ ]. ..

Article Title: Screening and Evaluation of New Hydroxymethylfurfural Oxidases for Furandicarboxylic Acid Production
Article Snippet: .. The codifying DNA sequences of the HMFOs from Methylovorus sp. M668, P. nitroreducens , Pseudomonas sp. 11/12A, Xanthobacter sp. 126, and Bradyrhizobium arachidis (NCBI accession numbers WP_013440946 , WP_024766380 , WP_047529632 , WP_024277017 , and WP_092217059 , respectively) were manually optimized for E. coli expression and synthesized by ATG:biosynthetics. .. The sequences were subcloned from the pGH vector into the pET23b(+) or pET28a(+) expression vector.

Article Title: Catalytic surface radical in dye-decolorizing peroxidase: a computational, spectroscopic and site-directed mutagenesis study
Article Snippet: .. DyP production, activation and purification The DNA sequence coding mature DyP-I from A. auricula-judae (GenBank® accession number JQ650250) [ ] was synthesized (ATG:biosynthetics), expressed in E. coli , activated in vitro , and purified as described in [ ] (see the Supplementary Methods for details). .. Site-directed mutagenesis and chemical modification of DyP Simple DyP variants were produced by PCR using the pET23a-DyPI vector harbouring the mature protein-coding sequence of A. auricula-judae DyP as a template.

Mutagenesis:

Article Title: Desensitization and Internalization of Endothelin Receptor A
Article Snippet: .. The mutant receptor constructs ETA -4PD, ETA -6PD, ETA -8PD, ETA -10PD, ETA -PDZPD, ETA -6E, and ETA -14PD were generated by the exchange of a C-terminal sequence of ETA with a corresponding synthetically generated DNA sequence (ATG:biosynthetics, Merzhausen, Germany) containing the respective point mutations via EcoRI (in the coding sequence of ETA ) and NotI. .. All other constructs were derived from these plasmids by site-directed mutagenesis using the QuikChange Lightning kit (Agilent Technologies, Santa Clara, CA). mRuby ( )-tagged receptor constructs were created by amplification of the respective receptor DNA lacking the stop codon with the use of oligonucleotides 5′-CTCGAGTATTTCCTCAAATTTGCCTCAAGATGGA-3′ and 5′-AAGACCGGTCCGTTCATGCTGTCCTTATGGCTGCTC-3′ (ETA -WT and ETA -PDZPD) or 5′-AAGACCGGTCCGTTCATGGCGTCCTTATGGGCG-3′ (receptor constructs containing S421A and S425A mutations), thereby flanking the PCR product with XhoI and AgeI restriction sites.

Construct:

Article Title: Desensitization and Internalization of Endothelin Receptor A
Article Snippet: .. The mutant receptor constructs ETA -4PD, ETA -6PD, ETA -8PD, ETA -10PD, ETA -PDZPD, ETA -6E, and ETA -14PD were generated by the exchange of a C-terminal sequence of ETA with a corresponding synthetically generated DNA sequence (ATG:biosynthetics, Merzhausen, Germany) containing the respective point mutations via EcoRI (in the coding sequence of ETA ) and NotI. .. All other constructs were derived from these plasmids by site-directed mutagenesis using the QuikChange Lightning kit (Agilent Technologies, Santa Clara, CA). mRuby ( )-tagged receptor constructs were created by amplification of the respective receptor DNA lacking the stop codon with the use of oligonucleotides 5′-CTCGAGTATTTCCTCAAATTTGCCTCAAGATGGA-3′ and 5′-AAGACCGGTCCGTTCATGCTGTCCTTATGGCTGCTC-3′ (ETA -WT and ETA -PDZPD) or 5′-AAGACCGGTCCGTTCATGGCGTCCTTATGGGCG-3′ (receptor constructs containing S421A and S425A mutations), thereby flanking the PCR product with XhoI and AgeI restriction sites.

Purification:

Article Title: Catalytic surface radical in dye-decolorizing peroxidase: a computational, spectroscopic and site-directed mutagenesis study
Article Snippet: .. DyP production, activation and purification The DNA sequence coding mature DyP-I from A. auricula-judae (GenBank® accession number JQ650250) [ ] was synthesized (ATG:biosynthetics), expressed in E. coli , activated in vitro , and purified as described in [ ] (see the Supplementary Methods for details). .. Site-directed mutagenesis and chemical modification of DyP Simple DyP variants were produced by PCR using the pET23a-DyPI vector harbouring the mature protein-coding sequence of A. auricula-judae DyP as a template.

Activation Assay:

Article Title: Catalytic surface radical in dye-decolorizing peroxidase: a computational, spectroscopic and site-directed mutagenesis study
Article Snippet: .. DyP production, activation and purification The DNA sequence coding mature DyP-I from A. auricula-judae (GenBank® accession number JQ650250) [ ] was synthesized (ATG:biosynthetics), expressed in E. coli , activated in vitro , and purified as described in [ ] (see the Supplementary Methods for details). .. Site-directed mutagenesis and chemical modification of DyP Simple DyP variants were produced by PCR using the pET23a-DyPI vector harbouring the mature protein-coding sequence of A. auricula-judae DyP as a template.

Generated:

Article Title: Desensitization and Internalization of Endothelin Receptor A
Article Snippet: .. The mutant receptor constructs ETA -4PD, ETA -6PD, ETA -8PD, ETA -10PD, ETA -PDZPD, ETA -6E, and ETA -14PD were generated by the exchange of a C-terminal sequence of ETA with a corresponding synthetically generated DNA sequence (ATG:biosynthetics, Merzhausen, Germany) containing the respective point mutations via EcoRI (in the coding sequence of ETA ) and NotI. .. All other constructs were derived from these plasmids by site-directed mutagenesis using the QuikChange Lightning kit (Agilent Technologies, Santa Clara, CA). mRuby ( )-tagged receptor constructs were created by amplification of the respective receptor DNA lacking the stop codon with the use of oligonucleotides 5′-CTCGAGTATTTCCTCAAATTTGCCTCAAGATGGA-3′ and 5′-AAGACCGGTCCGTTCATGCTGTCCTTATGGCTGCTC-3′ (ETA -WT and ETA -PDZPD) or 5′-AAGACCGGTCCGTTCATGGCGTCCTTATGGGCG-3′ (receptor constructs containing S421A and S425A mutations), thereby flanking the PCR product with XhoI and AgeI restriction sites.

Expressing:

Article Title: Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod–Zwilch–ZW10 (RZZ) complex
Article Snippet: .. RZZ complex production The DNA sequences of human Zwilch and Rod were subcloned into a pACEbac1 or pFL expression vector (ATG Biosynthetics, Merzhausen, Germany). .. Expression of Zwilch with two His residues at its N-terminus considerably enhanced its expression levels.

Article Title: Defining HIV-1 Envelope N-Glycan Microdomains through Site-Specific Heterogeneity Profiles
Article Snippet: .. Both DNA sequences coding for respective gp160 protein were codon optimized, synthesized by a commercial supplier (ATG:biosynthetics, Merzhausen, Germany), and cloned in frame into expression plasmid pcDNA3.1. .. Both gp160 amino acid sequences are numbered according to the alignment with the HIV-1 HXB2 sequence (GenBank number ).

Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
Article Snippet: .. The synthetic genes were cloned into the expression vector pET17b, and the DNA sequences were determined by means of sequencing (ATG Biosynthetics). .. For more information on expression and purification of Der p 2/1 mosaic proteins, see the Methods section in this article’s Online Repository.

Article Title: Impact of glycan positioning on HIV-1 Env glycan shield density, function, and antibody recognition
Article Snippet: .. Both DNA sequences coding for the respective gp160 protein were codon-optimized, synthesized by commercial supplier (ATG:biosynthetics, Merzhausen, Germany), and cloned in frame into expression plasmid pcDNA3.1 (yielding pcDNA3.1-WEAU gp160 clones that were used for production of Env-pseudotyped HIV-1). .. Region corresponding to gp120 amino-acid sequences shown in are numbered according to the alignment with HIV-1HXB2 sequence (GenBank accession number K03455 ).

Article Title: Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date
Article Snippet: .. Then, the DNA sequences encoding the most probable amino-acid sequences for the four reconstructed nodes, together with the two alternative sequences from the CaPo subsets, were synthesized by ATG:biosynthetics (Merzhausen, Germany) after optimizing the codon usage for high expression in E. coli using OPTIMIZER [ ]. ..

Article Title: Screening and Evaluation of New Hydroxymethylfurfural Oxidases for Furandicarboxylic Acid Production
Article Snippet: .. The codifying DNA sequences of the HMFOs from Methylovorus sp. M668, P. nitroreducens , Pseudomonas sp. 11/12A, Xanthobacter sp. 126, and Bradyrhizobium arachidis (NCBI accession numbers WP_013440946 , WP_024766380 , WP_047529632 , WP_024277017 , and WP_092217059 , respectively) were manually optimized for E. coli expression and synthesized by ATG:biosynthetics. .. The sequences were subcloned from the pGH vector into the pET23b(+) or pET28a(+) expression vector.

Sequencing:

Article Title: Desensitization and Internalization of Endothelin Receptor A
Article Snippet: .. The mutant receptor constructs ETA -4PD, ETA -6PD, ETA -8PD, ETA -10PD, ETA -PDZPD, ETA -6E, and ETA -14PD were generated by the exchange of a C-terminal sequence of ETA with a corresponding synthetically generated DNA sequence (ATG:biosynthetics, Merzhausen, Germany) containing the respective point mutations via EcoRI (in the coding sequence of ETA ) and NotI. .. All other constructs were derived from these plasmids by site-directed mutagenesis using the QuikChange Lightning kit (Agilent Technologies, Santa Clara, CA). mRuby ( )-tagged receptor constructs were created by amplification of the respective receptor DNA lacking the stop codon with the use of oligonucleotides 5′-CTCGAGTATTTCCTCAAATTTGCCTCAAGATGGA-3′ and 5′-AAGACCGGTCCGTTCATGCTGTCCTTATGGCTGCTC-3′ (ETA -WT and ETA -PDZPD) or 5′-AAGACCGGTCCGTTCATGGCGTCCTTATGGGCG-3′ (receptor constructs containing S421A and S425A mutations), thereby flanking the PCR product with XhoI and AgeI restriction sites.

Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
Article Snippet: .. The synthetic genes were cloned into the expression vector pET17b, and the DNA sequences were determined by means of sequencing (ATG Biosynthetics). .. For more information on expression and purification of Der p 2/1 mosaic proteins, see the Methods section in this article’s Online Repository.

Article Title: Catalytic surface radical in dye-decolorizing peroxidase: a computational, spectroscopic and site-directed mutagenesis study
Article Snippet: .. DyP production, activation and purification The DNA sequence coding mature DyP-I from A. auricula-judae (GenBank® accession number JQ650250) [ ] was synthesized (ATG:biosynthetics), expressed in E. coli , activated in vitro , and purified as described in [ ] (see the Supplementary Methods for details). .. Site-directed mutagenesis and chemical modification of DyP Simple DyP variants were produced by PCR using the pET23a-DyPI vector harbouring the mature protein-coding sequence of A. auricula-judae DyP as a template.

Plasmid Preparation:

Article Title: Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod–Zwilch–ZW10 (RZZ) complex
Article Snippet: .. RZZ complex production The DNA sequences of human Zwilch and Rod were subcloned into a pACEbac1 or pFL expression vector (ATG Biosynthetics, Merzhausen, Germany). .. Expression of Zwilch with two His residues at its N-terminus considerably enhanced its expression levels.

Article Title: Defining HIV-1 Envelope N-Glycan Microdomains through Site-Specific Heterogeneity Profiles
Article Snippet: .. Both DNA sequences coding for respective gp160 protein were codon optimized, synthesized by a commercial supplier (ATG:biosynthetics, Merzhausen, Germany), and cloned in frame into expression plasmid pcDNA3.1. .. Both gp160 amino acid sequences are numbered according to the alignment with the HIV-1 HXB2 sequence (GenBank number ).

Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
Article Snippet: .. The synthetic genes were cloned into the expression vector pET17b, and the DNA sequences were determined by means of sequencing (ATG Biosynthetics). .. For more information on expression and purification of Der p 2/1 mosaic proteins, see the Methods section in this article’s Online Repository.

Article Title: Impact of glycan positioning on HIV-1 Env glycan shield density, function, and antibody recognition
Article Snippet: .. Both DNA sequences coding for the respective gp160 protein were codon-optimized, synthesized by commercial supplier (ATG:biosynthetics, Merzhausen, Germany), and cloned in frame into expression plasmid pcDNA3.1 (yielding pcDNA3.1-WEAU gp160 clones that were used for production of Env-pseudotyped HIV-1). .. Region corresponding to gp120 amino-acid sequences shown in are numbered according to the alignment with HIV-1HXB2 sequence (GenBank accession number K03455 ).