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2-O-methylmagnolol (MM1)/magnolol and sorafenib show a synergistic anti-hepatocellular carcinoma effect. (A,B) Huh7 and <t>HepG2</t> cells were treated with 40 μM magnolol/MM1 and 3.5 μM sorafenib, individually, or in combination. The cell proliferation status was analyzed using an xCELLigence Real-Time Cell Analyzer. The data are expressed as the mean ± standard deviation from three independent experiments. (C) The cell cycle status in Huh7 cells treated with magnolol/MM1/sorafenib was examined by flow cytometry. (D) Effects of magnolol/MM1 and sorafenib alone or in combination on cell proliferation in human fibroblasts.
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1) Product Images from "2-O-Methylmagnolol, a Magnolol Derivative, Suppresses Hepatocellular Carcinoma Progression via Inhibiting Class I Histone Deacetylase Expression"

Article Title: 2-O-Methylmagnolol, a Magnolol Derivative, Suppresses Hepatocellular Carcinoma Progression via Inhibiting Class I Histone Deacetylase Expression

Journal: Frontiers in Oncology

doi: 10.3389/fonc.2020.01319

2-O-methylmagnolol (MM1)/magnolol and sorafenib show a synergistic anti-hepatocellular carcinoma effect. (A,B) Huh7 and HepG2 cells were treated with 40 μM magnolol/MM1 and 3.5 μM sorafenib, individually, or in combination. The cell proliferation status was analyzed using an xCELLigence Real-Time Cell Analyzer. The data are expressed as the mean ± standard deviation from three independent experiments. (C) The cell cycle status in Huh7 cells treated with magnolol/MM1/sorafenib was examined by flow cytometry. (D) Effects of magnolol/MM1 and sorafenib alone or in combination on cell proliferation in human fibroblasts.
Figure Legend Snippet: 2-O-methylmagnolol (MM1)/magnolol and sorafenib show a synergistic anti-hepatocellular carcinoma effect. (A,B) Huh7 and HepG2 cells were treated with 40 μM magnolol/MM1 and 3.5 μM sorafenib, individually, or in combination. The cell proliferation status was analyzed using an xCELLigence Real-Time Cell Analyzer. The data are expressed as the mean ± standard deviation from three independent experiments. (C) The cell cycle status in Huh7 cells treated with magnolol/MM1/sorafenib was examined by flow cytometry. (D) Effects of magnolol/MM1 and sorafenib alone or in combination on cell proliferation in human fibroblasts.

Techniques Used: Standard Deviation, Flow Cytometry

2-O-methylmagnolol (MM1) and magnolol inhibit hepatocellular carcinoma cell migration and invasion by suppressing epithelial-mesenchymal transition (EMT). (A,B) Comparisons of migration capacities of Huh7 and HepG2 cells treated with magnolol or MM1 in transwell assays. (C,D) Invasion assays using Matrigel-coated polyethylene terephthalate membrane inserts. (E,G) Western blotting showing the expression of EMT-related proteins in Huh7 and HepG2 cells after treatment with magnolol and MM1. Quantitative results are shown in (F,H) . The results are shown as the mean of three independent experiments. Significant differences compared with the vehicle control groups, * p
Figure Legend Snippet: 2-O-methylmagnolol (MM1) and magnolol inhibit hepatocellular carcinoma cell migration and invasion by suppressing epithelial-mesenchymal transition (EMT). (A,B) Comparisons of migration capacities of Huh7 and HepG2 cells treated with magnolol or MM1 in transwell assays. (C,D) Invasion assays using Matrigel-coated polyethylene terephthalate membrane inserts. (E,G) Western blotting showing the expression of EMT-related proteins in Huh7 and HepG2 cells after treatment with magnolol and MM1. Quantitative results are shown in (F,H) . The results are shown as the mean of three independent experiments. Significant differences compared with the vehicle control groups, * p

Techniques Used: Migration, Western Blot, Expressing

2-O-methylmagnolol (MM1) and magnolol inhibit class I histone deacetylase (HDAC) expression in hepatocellular carcinoma cell lines. (A,C) Huh7 and HepG2 cells were treated with magnolol, MM1, or vehicle for 48 h. The expression levels of HDACs 1, 2, 3, and 8 were determined by Western blotting. Quantitative results are shown (B,D) . (E) The levels of acetylated histone H3 in HepG2 cells were examined by Western blotting. The quantitative results are shown in (F) . The measurement data are expressed as the mean ± standard deviation of three independent experiments. * p
Figure Legend Snippet: 2-O-methylmagnolol (MM1) and magnolol inhibit class I histone deacetylase (HDAC) expression in hepatocellular carcinoma cell lines. (A,C) Huh7 and HepG2 cells were treated with magnolol, MM1, or vehicle for 48 h. The expression levels of HDACs 1, 2, 3, and 8 were determined by Western blotting. Quantitative results are shown (B,D) . (E) The levels of acetylated histone H3 in HepG2 cells were examined by Western blotting. The quantitative results are shown in (F) . The measurement data are expressed as the mean ± standard deviation of three independent experiments. * p

Techniques Used: Histone Deacetylase Assay, Expressing, Western Blot, Standard Deviation

2-O-methylmagnolol (MM1) and magnolol induce the expression of the tumor-suppressor gene p21 and the acetylation of p53. (A) Huh7 and HepG2 cells were treated with the indicated concentrations of magnolol or MM1 for 48 h. The p21 RNA levels were examined by quantitative real-time reverse transcription-polymerase chain reaction. (B) Expression levels of p21 and downstream proteins in Huh7 cells were analyzed by Western blot using β-actin as an internal control. Quantitative results are shown in (C) . * p
Figure Legend Snippet: 2-O-methylmagnolol (MM1) and magnolol induce the expression of the tumor-suppressor gene p21 and the acetylation of p53. (A) Huh7 and HepG2 cells were treated with the indicated concentrations of magnolol or MM1 for 48 h. The p21 RNA levels were examined by quantitative real-time reverse transcription-polymerase chain reaction. (B) Expression levels of p21 and downstream proteins in Huh7 cells were analyzed by Western blot using β-actin as an internal control. Quantitative results are shown in (C) . * p

Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Compared to magnolol, 2-O-methylmagnolol (MM1) demonstrates a greater ability to inhibit hepatocellular carcinoma (HCC) cell growth. (A) Chemical structures of MM1 and magnolol. (B–F) Human skin fibroblasts, Huh 7, and HepG2 cells were treated with different concentrations (0, 25, 50, 75, and 100 μM) of magnolol or MM1, and the cell proliferation status was analyzed using an xCELLigence Real-Time Cell Analyzer. The results are shown as the mean ± standard deviation of three independent experiments. Significant differences compared with the vehicle groups, ** p
Figure Legend Snippet: Compared to magnolol, 2-O-methylmagnolol (MM1) demonstrates a greater ability to inhibit hepatocellular carcinoma (HCC) cell growth. (A) Chemical structures of MM1 and magnolol. (B–F) Human skin fibroblasts, Huh 7, and HepG2 cells were treated with different concentrations (0, 25, 50, 75, and 100 μM) of magnolol or MM1, and the cell proliferation status was analyzed using an xCELLigence Real-Time Cell Analyzer. The results are shown as the mean ± standard deviation of three independent experiments. Significant differences compared with the vehicle groups, ** p

Techniques Used: Standard Deviation

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Amplification:

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Polymerase Chain Reaction:

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Purification:

Article Title: Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution
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Article Title: First molecular identification of Vorticella sp. from freshwater shrimps in Tainan, Taiwan
Article Snippet: .. The cycling parameters for the ITS1-5.8SITS2 sequence were as follows: 10 min of initial denaturation at 94 °C, followed by 35 cycles of denaturation for 30 s at 94 °C, primer annealing for 30 s at 56 °C, and extension for 30 s at 72 °C; a final extension step was at 72 °C for 10 min. DNA-view stained PCR products separated on gel after electrophoresis in 1.2% agarose (DNA-view, BIOTOOLS CO., LTD, Taiwan) and purified using an EasyPure PCR/Gel extraction Kit (Bioman Scientific Co. Ltd, New Taipei City, Taiwan). ..

Electrophoresis:

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Sequencing:

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Western Blot:

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Reverse Transcription Polymerase Chain Reaction:

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Lysis:

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Staining:

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