Structured Review

Biotools B & M Labs dna polymerase
Dna Polymerase, supplied by Biotools B & M Labs, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna polymerase/product/Biotools B & M Labs
Average 96 stars, based on 14 article reviews
Price from $9.99 to $1999.99
dna polymerase - by Bioz Stars, 2020-03
96/100 stars

Images

Related Articles

Clone Assay:

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: Suchwise, two «replicate» 96-well plates are prepared simultaneously: the first one to be used for actual screening by PCR ( screen plate ), and the second one serving as a handy living stock of all the clones that underwent PCR ( stock plate ), allowing for easy and error-free maintenance and expansion of the selected clones once screen PCR results become available. .. The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase.

Article Title: Allelic Variation, Alternative Splicing and Expression Analysis of Psy1 Gene in Hordeum chilense Roem. et Schult
Article Snippet: For the synthesis of first-strand cDNA, 4 µg of total RNA was reverse transcribed using oligo (dT) primer and M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA) in 20 µl total volume according to the manufacturer's instructions. cDNA samples were diluted with additional 230 µl of water. cDNA PCR amplifications with primer combinations HcPsy1-CDS3F/HcPsy1-CDS5R (primer pair 1) or HcPsy1-CDS4F/HcPsy1-CDS4R (primer pair 2) were performed in 25 µl reactions consisting of 0.625 units of DNA polymerase (Biotools B & M Labs, Madrid), 1X PCR buffer, 2 mM MgCl2 , 320 µM dNTPs (Promega, Madison, WI, USA), 1 M betaine (Sigma-Aldrich, St. Louis, MO), 0.6 µM of each primer and 1.2 µl of the cDNA dilution. .. Full-length Psy1 cDNA sequences were obtained by cloning and sequencing the obtained PCR fragments as specified before.

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: Suchwise, two «replicate» 96-well plates are prepared simultaneously: the first one to be used for actual screening by PCR (screen plate ), and the second one serving as a handy living stock of all the clones that underwent PCR (stock plate ), allowing for easy and error-free maintenance and expansion of the selected clones once screen PCR results become available. .. The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase.

Amplification:

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: DNA was amplified with the following primers: [5′-TAG GCT GAA AGG GGG AAA GC-3′ (sense), 5′-CTG TTC ACC TCA ACT GCT CC-3′ (antisense)]. .. PCR was carried out in a volume of 15 μ L containing 100 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain), 3.5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S.A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland).

Article Title: DNA sequence analysis suggests that cytb-nd1 PCR-RFLP may not be applicable to sandfly species identification throughout the Mediterranean region
Article Snippet: The mitochondrial DNA target encompassing the cytb -nd1 region was amplified according to Latrofa et al. ( ) with minor modifications. .. Two microliters of DNA was used in a 25-μL final volume PCR reaction, including standard reaction buffer 2 mM MgCl2 , 0.2 mM each dNTP, and 0.7 U of Thermus sp. DNA polymerase (Biotools, B & M Labs, Spain), and 15 pmol of each primer PhleF (5′-AAT AAA TTA GGA GGA GTA ATT GC-3′) and PhleR (5′-GCC TCG AWT TCG WTT ATG ATA AAT T-3′) (Sigma-Genosys).

Article Title: Allelic Variation, Alternative Splicing and Expression Analysis of Psy1 Gene in Hordeum chilense Roem. et Schult
Article Snippet: Paragraph title: Amplification of CDS and full-length genomic sequence ... For the synthesis of first-strand cDNA, 4 µg of total RNA was reverse transcribed using oligo (dT) primer and M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA) in 20 µl total volume according to the manufacturer's instructions. cDNA samples were diluted with additional 230 µl of water. cDNA PCR amplifications with primer combinations HcPsy1-CDS3F/HcPsy1-CDS5R (primer pair 1) or HcPsy1-CDS4F/HcPsy1-CDS4R (primer pair 2) were performed in 25 µl reactions consisting of 0.625 units of DNA polymerase (Biotools B & M Labs, Madrid), 1X PCR buffer, 2 mM MgCl2 , 320 µM dNTPs (Promega, Madison, WI, USA), 1 M betaine (Sigma-Aldrich, St. Louis, MO), 0.6 µM of each primer and 1.2 µl of the cDNA dilution.

Article Title: Increased AGE-RAGE ratio in idiopathic pulmonary fibrosis
Article Snippet: PCR was performed with DNA polymerase (Biotools B & M Labs, Spain) following the manufacturer’s advice; cDNA was mixed with RAGE primers (164 bp): sense 5′-CAGGACCAGGGAACCTACAG-3′ and antisense 5′-CATGTGTTGGGGGCTATCTT-3′; and β-actin primers (302 bp) were used as a housekeeping gene: sense 5′-GCACTCTTCCAGCCTTCCTTCC-3′ and antisense 5′-TGCTTGCTGATCCACATCTGCT-3′ (Sigma-Aldrich). .. Amplified samples ran in 2 % agarose gel electrophoresis with ethidium bromide.

Article Title: Hordeum chilense genome, a useful tool to investigate the endosperm yellow pigment content in the Triticeae
Article Snippet: .. PCR amplification and polymorphism detection PCR amplifications were performed in 25 μl reactions consisting of 0.625 units of DNA polymerase (Biotools B & M Labs, Madrid, Spain), 1× PCR buffer, 1.6 mM MgCl2 , 320 mM dNTPs (Promega, Madison, WI, USA), 0.6 mM of each primer and 50 ng of genomic DNA. .. PCR were carried out as follows: 5 min at 94°C, 35 cycles of 30 s at 94°C, 30 s at 58°C (54°C for Zds ) and 1 min at 72°C followed by 7 min at 72°C.

Article Title: Identification of circo-like virus-Brazil genomic sequences in raw sewage from the metropolitan area of São Paulo: evidence of circulation two and three years after the first detection
Article Snippet: DNA polymerase (Biotools B & M Labs) was employed in all PCR assays (each with 35 cycles), and the annealing temperatures were calculated using the PrimerSelect program in the Lasergene package (DNASTAR Inc). .. All the first-round PCR products were reamplified (5 µL of the first-round amplification product was added to the second-round amplification mixture) because the expected bands were visible only after a second round of PCR.

Article Title: Morphological and molecular identification to secure cultivar maintenance and management of self-sterile Rubus arcticus
Article Snippet: The PCR reactions were performed in a 10-μL reaction mixture containing 20–30 ng template DNA, 75 m m Tris-HCl (pH 9·0), 2 m m MgCl2 , 50 m m KCl, 20 m m (NH4 )2 SO4 , 0·2 µ m of each forward and reverse primer, 200 µ m dNTP and 0·5 U DNA polymerase (Biotools, B & M Labs, S.A., Spain). .. Amplification was carried out in 96-well plates in a PTC-100 Programmable Thermal Controller (MJ Research Inc., Bio-Rad Laboratories, Hercules, CA, USA).

Article Title: Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (Pinus sylvestris L.) Seedlings Using UPLC–Orbitrap–MS
Article Snippet: The nucleotide sequence of the Internal Transcribed Spacer (ITS) region of fungal ribosomal DNA (rDNA) was analysed in Macrogen Inc. (Amsterdam) from polymerase chain reaction (PCR) product amplified with ITS1 and ITS4 primer pair [ ]. .. The reaction mixture of 50 µL included, 10x enzyme buffer (Biotools B & M Labs, S.A. Madrid, Spain), 0.5 µM each primers, 0.2 µM dNTP mix, DNA Polymerase (5 U/µL) (Biotools B & M Labs, S.A. Madrid, Spain) and 1 µL DNA template.

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: DNA was amplified with the following primers: [5′-ACT AGG CCT CAC CTG ATA CG-3′ (sense), 5′-AGG TGT CGA TTT GCA GTG AC-3′ (antisense)] as a product with 646 bp length. .. PCR was carried out in a volume of 15 μ L containing 50 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S. A., Madrid, Spain), 5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S. A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland).

Article Title: Panmixia and dispersal from the Mediterranean Basin to Macaronesian Islands of a macrolichen species
Article Snippet: Paragraph title: MAT genes: primers design and amplification ... To identify the mating-type idiomorph of each sample, we performed multiplexed PCR reactions in a total volume of 25 μL with 0.65 μL of each MAT1-1 primer (10 μM), 0.6 μL of each MAT1-2 primer (10 μM), 0.4 μL dNTP’s (10 mM; Biotools B & M Labs, Madrid, Spain) and 0.5 μL DNA polymerase (1 U/μL, Biotools B & M Labs, Madrid, Spain) and 2.5 μL of 10–50 ng DNA.

Filtration:

Article Title: Identification of circo-like virus-Brazil genomic sequences in raw sewage from the metropolitan area of São Paulo: evidence of circulation two and three years after the first detection
Article Snippet: Viruses were concentrated from 15 L of raw sewage and 100 L of reclaimed water by filtration through Zeta Plus 60S positively charged microporous filter membranes (Cuno Inc.) followed by ultracentrifugation as previously described ( , , resulting in final concentration factors of 8,000 and 50,000 times, respectively ( . .. DNA polymerase (Biotools B & M Labs) was employed in all PCR assays (each with 35 cycles), and the annealing temperatures were calculated using the PrimerSelect program in the Lasergene package (DNASTAR Inc).

Electrophoresis:

Article Title: Morphological and molecular identification to secure cultivar maintenance and management of self-sterile Rubus arcticus
Article Snippet: The PCR reactions were performed in a 10-μL reaction mixture containing 20–30 ng template DNA, 75 m m Tris-HCl (pH 9·0), 2 m m MgCl2 , 50 m m KCl, 20 m m (NH4 )2 SO4 , 0·2 µ m of each forward and reverse primer, 200 µ m dNTP and 0·5 U DNA polymerase (Biotools, B & M Labs, S.A., Spain). .. Labelled (Hex, Tet or Fam) amplification products were detected by capillary electrophoresis using a MegaBACE 1000 DNA sequencer with ET400-R as size standard (GE Healthcare, Little Chalfont, UK).

Article Title: CIP2A Promotes Proliferation of Spermatogonial Progenitor Cells and Spermatogenesis in Mice
Article Snippet: Genotyping One microgram genomic DNA from mouse ear was added to the 50 µl PCR containing 75 mM Tris HCl (pH 9.0); 50 mM KCl; 2 mM MgCl2; 20 mM (NH4)2SO4; 0.2 µM of each primer, 0.2 mM dNTPs, and 2.5 U DNA polymerase (Biotools Native DNA Polymerase, BIOTOOLS B & M Labs, S.A., Spain). .. The resulting PCR products were analyzed by electrophoresis on 2% agarose gel, and the fragments were UV visualized with ethidium bromide.

Incubation:

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: The stock plate was covered with a lid and placed onto a thermoshaker (Thermomixer Comfort, Eppendorf AG, Germany) for incubation at +37°C and 400 RPM for 8-12 h; after that the plate was transferred to +4°C and kept until further use. .. The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase.

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: The restriction was performed in a volume of 12 μ L containing 7 μ L of the PCR product, 10x NeBuffer 4, and 4 U of BsmFI enzyme (New England Biolabs, Hitchin, UK) and incubated for 4 hours at 65°C. .. PCR was carried out in a volume of 15 μ L containing 100 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain), 3.5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S.A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland).

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: The stock plate was covered with a lid and placed onto a thermoshaker (Thermomixer Comfort, Eppendorf AG, Germany) for incubation at +37°C and 400 RPM for 8-12 h; after that the plate was transferred to +4°C and kept until further use. .. The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase.

Modification:

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: VNTR (IL-4 70-bp repeat) polymorphism in intron 3 of the IL-4 gene was detected by a modification of the PCR method described by Mout et al. [ ]. .. PCR was carried out in a volume of 15 μ L containing 100 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain), 3.5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S.A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland).

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: The IL-4 SNP polymorphism at position -590C/T (rs2243250) was genotyped by PCR-RFLP analysis according to previously published methods [ ] with slight modification. .. PCR was carried out in a volume of 15 μ L containing 50 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S. A., Madrid, Spain), 5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S. A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland).

Chloramphenicol Acetyltransferase Assay:

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: A toothpick with bacteria attached to its tip was initially dipped for 1-2 s into one well of 96 - well PCR plate (4titude UK; Cat4ti-0740) prefilled with 5 μl of ultrapure water supplemented with 20 μg/ml of RNase A (Sigma, Cat# R5503), then removed and dipped for another few seconds into a corresponding single well of a 96 - well polystyrene round-bottom plate for suspension cultures (Greiner; Cat#650185) prefilled with 150 μl/well of LB medium supplemented with appropriate selection agent. .. The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase.

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: A toothpick with bacteria attached to its tip was initially dipped for 1-2 s into one well of 96 - well PCR plate (4titude UK; Cat4ti-0740) prefilled with 5 μl of ultrapure water supplemented with 20 μg/ml of RNase A (Sigma, Cat# R5503), then removed and dipped for another few seconds into a corresponding single well of a 96 - well polystyrene round-bottom plate for suspension cultures (Greiner; Cat#650185) prefilled with 150 μl/well of LB medium supplemented with appropriate selection agent. .. The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase.

Sequencing:

Article Title: Allelic Variation, Alternative Splicing and Expression Analysis of Psy1 Gene in Hordeum chilense Roem. et Schult
Article Snippet: Paragraph title: Amplification of CDS and full-length genomic sequence ... For the synthesis of first-strand cDNA, 4 µg of total RNA was reverse transcribed using oligo (dT) primer and M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA) in 20 µl total volume according to the manufacturer's instructions. cDNA samples were diluted with additional 230 µl of water. cDNA PCR amplifications with primer combinations HcPsy1-CDS3F/HcPsy1-CDS5R (primer pair 1) or HcPsy1-CDS4F/HcPsy1-CDS4R (primer pair 2) were performed in 25 µl reactions consisting of 0.625 units of DNA polymerase (Biotools B & M Labs, Madrid), 1X PCR buffer, 2 mM MgCl2 , 320 µM dNTPs (Promega, Madison, WI, USA), 1 M betaine (Sigma-Aldrich, St. Louis, MO), 0.6 µM of each primer and 1.2 µl of the cDNA dilution.

Article Title: Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (Pinus sylvestris L.) Seedlings Using UPLC–Orbitrap–MS
Article Snippet: The nucleotide sequence of the Internal Transcribed Spacer (ITS) region of fungal ribosomal DNA (rDNA) was analysed in Macrogen Inc. (Amsterdam) from polymerase chain reaction (PCR) product amplified with ITS1 and ITS4 primer pair [ ]. .. The reaction mixture of 50 µL included, 10x enzyme buffer (Biotools B & M Labs, S.A. Madrid, Spain), 0.5 µM each primers, 0.2 µM dNTP mix, DNA Polymerase (5 U/µL) (Biotools B & M Labs, S.A. Madrid, Spain) and 1 µL DNA template.

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: Genetic Analysis The GenBank accession number was AF395008 for the reference genomic sequence used for IL-4 alignments. .. PCR was carried out in a volume of 15 μ L containing 50 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S. A., Madrid, Spain), 5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S. A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland).

DNA Extraction:

Article Title: DNA sequence analysis suggests that cytb-nd1 PCR-RFLP may not be applicable to sandfly species identification throughout the Mediterranean region
Article Snippet: Paragraph title: DNA extraction and cytb-nd1 PCR from assembly 1 ... Two microliters of DNA was used in a 25-μL final volume PCR reaction, including standard reaction buffer 2 mM MgCl2 , 0.2 mM each dNTP, and 0.7 U of Thermus sp. DNA polymerase (Biotools, B & M Labs, Spain), and 15 pmol of each primer PhleF (5′-AAT AAA TTA GGA GGA GTA ATT GC-3′) and PhleR (5′-GCC TCG AWT TCG WTT ATG ATA AAT T-3′) (Sigma-Genosys).

Article Title: Vitamin D receptor gene polymorphisms and susceptibility of hand osteoarthritis in Finnish women
Article Snippet: Genotyping analysis All DNA samples were extracted from lymphocytes by a DNA extraction kit (PUREGENE® DNA Purification Kit; Gentra Systems, Plymouth, MN, USA). .. Briefly, the PCR reactions were set up as follows: 50–100 ng template, 1 U DNA polymerase (Biotools; B & M Labs, SA, Madrid, Spain), 0.2 mM dNTPs, 0.5 μM each primer and 1.5 mM MgCl2 in the magnesium-free PCR buffer (Biotools; B & M Labs, SA).

MTT Assay:

Article Title: Morphological and molecular identification to secure cultivar maintenance and management of self-sterile Rubus arcticus
Article Snippet: 26, 126, 157, 223, 262, 277 and 280, developed for raspberry SSR loci , were initially tested with 24 DNA samples extracted from arctic bramble cultivars and open-pollinated arctic bramble and hybrid arctic bramble seedlings at Agrifood Research Finland MTT. .. The PCR reactions were performed in a 10-μL reaction mixture containing 20–30 ng template DNA, 75 m m Tris-HCl (pH 9·0), 2 m m MgCl2 , 50 m m KCl, 20 m m (NH4 )2 SO4 , 0·2 µ m of each forward and reverse primer, 200 µ m dNTP and 0·5 U DNA polymerase (Biotools, B & M Labs, S.A., Spain).

Screening Assay:

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase. .. The primers for the screening assay were purposely designed to either produce no product in the absence of the correct insert (e.g., with one primer landing in the backbone and the other having a complementary region within the insert) or to produce products of sensibly different size for cases of no insert/insert present.

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase. .. The primers for the screening assay were purposely designed to either produce no product in the absence of the correct insert (e.g., with one primer landing in the backbone and the other having a complementary region within the insert) or to produce products of sensibly different size for cases of no insert/insert present.

Isolation:

Article Title: Allelic Variation, Alternative Splicing and Expression Analysis of Psy1 Gene in Hordeum chilense Roem. et Schult
Article Snippet: Total RNA was isolated from seeds at 18 and 28 DPA in lines H1 and H7 and from leaves of lines H1, H7 and H16 using the TRIzol® Reagent (Invitrogen, Carlsbad, CA) according to manufacturer's instructions. .. For the synthesis of first-strand cDNA, 4 µg of total RNA was reverse transcribed using oligo (dT) primer and M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA) in 20 µl total volume according to the manufacturer's instructions. cDNA samples were diluted with additional 230 µl of water. cDNA PCR amplifications with primer combinations HcPsy1-CDS3F/HcPsy1-CDS5R (primer pair 1) or HcPsy1-CDS4F/HcPsy1-CDS4R (primer pair 2) were performed in 25 µl reactions consisting of 0.625 units of DNA polymerase (Biotools B & M Labs, Madrid), 1X PCR buffer, 2 mM MgCl2 , 320 µM dNTPs (Promega, Madison, WI, USA), 1 M betaine (Sigma-Aldrich, St. Louis, MO), 0.6 µM of each primer and 1.2 µl of the cDNA dilution.

Article Title: Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (Pinus sylvestris L.) Seedlings Using UPLC–Orbitrap–MS
Article Snippet: Paragraph title: 3.2. Endophytic Fungi Isolation and Identification ... The reaction mixture of 50 µL included, 10x enzyme buffer (Biotools B & M Labs, S.A. Madrid, Spain), 0.5 µM each primers, 0.2 µM dNTP mix, DNA Polymerase (5 U/µL) (Biotools B & M Labs, S.A. Madrid, Spain) and 1 µL DNA template.

Size-exclusion Chromatography:

Article Title: Panmixia and dispersal from the Mediterranean Basin to Macaronesian Islands of a macrolichen species
Article Snippet: To identify the mating-type idiomorph of each sample, we performed multiplexed PCR reactions in a total volume of 25 μL with 0.65 μL of each MAT1-1 primer (10 μM), 0.6 μL of each MAT1-2 primer (10 μM), 0.4 μL dNTP’s (10 mM; Biotools B & M Labs, Madrid, Spain) and 0.5 μL DNA polymerase (1 U/μL, Biotools B & M Labs, Madrid, Spain) and 2.5 μL of 10–50 ng DNA. .. PCR reactions were performed with an initial denaturalization at 94 °C for 4 min, followed by 10 cycles of denaturalization step at 95 °C for 10 sec, annealing at 55 to 50 °C for 20 sec and extension at 72 °C for 30 sec, followed by 30 additional cycles with annealing temperature of 50 °C for 20 sec and an additional extension at 72 °C for 5 min. We tested if both idiomorphs were evenly distributed in the entire area and in each individual sampling locality with χ2 tests.

Article Title: CIP2A Promotes Proliferation of Spermatogonial Progenitor Cells and Spermatogenesis in Mice
Article Snippet: Genotyping One microgram genomic DNA from mouse ear was added to the 50 µl PCR containing 75 mM Tris HCl (pH 9.0); 50 mM KCl; 2 mM MgCl2; 20 mM (NH4)2SO4; 0.2 µM of each primer, 0.2 mM dNTPs, and 2.5 U DNA polymerase (Biotools Native DNA Polymerase, BIOTOOLS B & M Labs, S.A., Spain). .. The DNA was denatured at 96°C for 4 min, followed by PCR at 96 C for 45 sec, 57 C for 30 sec, and 72 C for 1 min for 25 cycles and completed by a final elongation step at 72 C for 10 min.

Polymerase Chain Reaction:

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: Paragraph title: Bacterial colonies screening by PCR ... The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase.

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: .. PCR was carried out in a volume of 15 μ L containing 100 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain), 3.5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S.A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland). ..

Article Title: DNA sequence analysis suggests that cytb-nd1 PCR-RFLP may not be applicable to sandfly species identification throughout the Mediterranean region
Article Snippet: .. Two microliters of DNA was used in a 25-μL final volume PCR reaction, including standard reaction buffer 2 mM MgCl2 , 0.2 mM each dNTP, and 0.7 U of Thermus sp. DNA polymerase (Biotools, B & M Labs, Spain), and 15 pmol of each primer PhleF (5′-AAT AAA TTA GGA GGA GTA ATT GC-3′) and PhleR (5′-GCC TCG AWT TCG WTT ATG ATA AAT T-3′) (Sigma-Genosys). .. Amplification was performed on a 9800 Fast Thermal Cycler (Applied Biosystems), standard ramp enabled, with the following conditions: initial denaturation of 5 min at 94 °C, 40 cycles at 94 °C for 1 min, 52 °C for 1 min, and 72 °C for 1 min, and final extension at 72 °C for 10 min. All the amplified products were resolved on 2 % agarose gels stained with Pronasafe Nucleic Acid Staining (Laboratorios CONDA, Spain) and visualized under UV light.

Article Title: Allelic Variation, Alternative Splicing and Expression Analysis of Psy1 Gene in Hordeum chilense Roem. et Schult
Article Snippet: .. For the synthesis of first-strand cDNA, 4 µg of total RNA was reverse transcribed using oligo (dT) primer and M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA) in 20 µl total volume according to the manufacturer's instructions. cDNA samples were diluted with additional 230 µl of water. cDNA PCR amplifications with primer combinations HcPsy1-CDS3F/HcPsy1-CDS5R (primer pair 1) or HcPsy1-CDS4F/HcPsy1-CDS4R (primer pair 2) were performed in 25 µl reactions consisting of 0.625 units of DNA polymerase (Biotools B & M Labs, Madrid), 1X PCR buffer, 2 mM MgCl2 , 320 µM dNTPs (Promega, Madison, WI, USA), 1 M betaine (Sigma-Aldrich, St. Louis, MO), 0.6 µM of each primer and 1.2 µl of the cDNA dilution. ..

Article Title: Increased AGE-RAGE ratio in idiopathic pulmonary fibrosis
Article Snippet: .. PCR was performed with DNA polymerase (Biotools B & M Labs, Spain) following the manufacturer’s advice; cDNA was mixed with RAGE primers (164 bp): sense 5′-CAGGACCAGGGAACCTACAG-3′ and antisense 5′-CATGTGTTGGGGGCTATCTT-3′; and β-actin primers (302 bp) were used as a housekeeping gene: sense 5′-GCACTCTTCCAGCCTTCCTTCC-3′ and antisense 5′-TGCTTGCTGATCCACATCTGCT-3′ (Sigma-Aldrich). .. Amplified samples ran in 2 % agarose gel electrophoresis with ethidium bromide.

Article Title: Hordeum chilense genome, a useful tool to investigate the endosperm yellow pigment content in the Triticeae
Article Snippet: .. PCR amplification and polymorphism detection PCR amplifications were performed in 25 μl reactions consisting of 0.625 units of DNA polymerase (Biotools B & M Labs, Madrid, Spain), 1× PCR buffer, 1.6 mM MgCl2 , 320 mM dNTPs (Promega, Madison, WI, USA), 0.6 mM of each primer and 50 ng of genomic DNA. .. PCR were carried out as follows: 5 min at 94°C, 35 cycles of 30 s at 94°C, 30 s at 58°C (54°C for Zds ) and 1 min at 72°C followed by 7 min at 72°C.

Article Title: Identification of circo-like virus-Brazil genomic sequences in raw sewage from the metropolitan area of São Paulo: evidence of circulation two and three years after the first detection
Article Snippet: .. DNA polymerase (Biotools B & M Labs) was employed in all PCR assays (each with 35 cycles), and the annealing temperatures were calculated using the PrimerSelect program in the Lasergene package (DNASTAR Inc). .. All the first-round PCR products were reamplified (5 µL of the first-round amplification product was added to the second-round amplification mixture) because the expected bands were visible only after a second round of PCR.

Article Title: Morphological and molecular identification to secure cultivar maintenance and management of self-sterile Rubus arcticus
Article Snippet: .. The PCR reactions were performed in a 10-μL reaction mixture containing 20–30 ng template DNA, 75 m m Tris-HCl (pH 9·0), 2 m m MgCl2 , 50 m m KCl, 20 m m (NH4 )2 SO4 , 0·2 µ m of each forward and reverse primer, 200 µ m dNTP and 0·5 U DNA polymerase (Biotools, B & M Labs, S.A., Spain). .. Amplification was carried out in 96-well plates in a PTC-100 Programmable Thermal Controller (MJ Research Inc., Bio-Rad Laboratories, Hercules, CA, USA).

Article Title: Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (Pinus sylvestris L.) Seedlings Using UPLC–Orbitrap–MS
Article Snippet: The nucleotide sequence of the Internal Transcribed Spacer (ITS) region of fungal ribosomal DNA (rDNA) was analysed in Macrogen Inc. (Amsterdam) from polymerase chain reaction (PCR) product amplified with ITS1 and ITS4 primer pair [ ]. .. The reaction mixture of 50 µL included, 10x enzyme buffer (Biotools B & M Labs, S.A. Madrid, Spain), 0.5 µM each primers, 0.2 µM dNTP mix, DNA Polymerase (5 U/µL) (Biotools B & M Labs, S.A. Madrid, Spain) and 1 µL DNA template.

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: .. PCR was carried out in a volume of 15 μ L containing 50 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S. A., Madrid, Spain), 5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S. A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland). ..

Article Title: Panmixia and dispersal from the Mediterranean Basin to Macaronesian Islands of a macrolichen species
Article Snippet: .. To identify the mating-type idiomorph of each sample, we performed multiplexed PCR reactions in a total volume of 25 μL with 0.65 μL of each MAT1-1 primer (10 μM), 0.6 μL of each MAT1-2 primer (10 μM), 0.4 μL dNTP’s (10 mM; Biotools B & M Labs, Madrid, Spain) and 0.5 μL DNA polymerase (1 U/μL, Biotools B & M Labs, Madrid, Spain) and 2.5 μL of 10–50 ng DNA. .. PCR reactions were performed with an initial denaturalization at 94 °C for 4 min, followed by 10 cycles of denaturalization step at 95 °C for 10 sec, annealing at 55 to 50 °C for 20 sec and extension at 72 °C for 30 sec, followed by 30 additional cycles with annealing temperature of 50 °C for 20 sec and an additional extension at 72 °C for 5 min. We tested if both idiomorphs were evenly distributed in the entire area and in each individual sampling locality with χ2 tests.

Article Title: CIP2A Promotes Proliferation of Spermatogonial Progenitor Cells and Spermatogenesis in Mice
Article Snippet: .. Genotyping One microgram genomic DNA from mouse ear was added to the 50 µl PCR containing 75 mM Tris HCl (pH 9.0); 50 mM KCl; 2 mM MgCl2; 20 mM (NH4)2SO4; 0.2 µM of each primer, 0.2 mM dNTPs, and 2.5 U DNA polymerase (Biotools Native DNA Polymerase, BIOTOOLS B & M Labs, S.A., Spain). .. The DNA was denatured at 96°C for 4 min, followed by PCR at 96 C for 45 sec, 57 C for 30 sec, and 72 C for 1 min for 25 cycles and completed by a final elongation step at 72 C for 10 min.

Activated Clotting Time Assay:

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: DNA was amplified with the following primers: [5′-TAG GCT GAA AGG GGG AAA GC-3′ (sense), 5′-CTG TTC ACC TCA ACT GCT CC-3′ (antisense)]. .. PCR was carried out in a volume of 15 μ L containing 100 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain), 3.5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S.A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland).

Selection:

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: A toothpick with bacteria attached to its tip was initially dipped for 1-2 s into one well of 96 - well PCR plate (4titude UK; Cat#4ti-0740) prefilled with 5 μl of ultrapure water supplemented with 20 μg/ml of RNase A (Sigma, Cat# R5503), then removed and dipped for another few seconds into a corresponding single well of a 96 - well polystyrene round-bottom plate for suspension cultures (Greiner; Cat#650185) prefilled with 150 μl/well of LB medium supplemented with appropriate selection agent. .. The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase.

Article Title: Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
Article Snippet: A toothpick with bacteria attached to its tip was initially dipped for 1-2 s into one well of 96 - well PCR plate (4titude UK; Cat#4ti-0740) prefilled with 5 μl of ultrapure water supplemented with 20 μg/ml of RNase A (Sigma, Cat# R5503), then removed and dipped for another few seconds into a corresponding single well of a 96 - well polystyrene round-bottom plate for suspension cultures (Greiner; Cat#650185) prefilled with 150 μl/well of LB medium supplemented with appropriate selection agent. .. The screen plate was further spiked with 10 μl/well of master mix, yielding a final reactions volume of 15 μl/well, containing 0.01U/μl of DNA polymerase (Biotools, B & M Labs, S.A., Cat# 10002-4100), 333 μM of dNTPs, 0.167 μM of forward and reverse primers in 1x standard buffer for the polymerase.

Agarose Gel Electrophoresis:

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: The PCR products were then digested with BsmFI restriction enzyme and separated on 3% agarose gel. .. PCR was carried out in a volume of 15 μ L containing 100 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain), 3.5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S.A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland).

Article Title: Increased AGE-RAGE ratio in idiopathic pulmonary fibrosis
Article Snippet: PCR was performed with DNA polymerase (Biotools B & M Labs, Spain) following the manufacturer’s advice; cDNA was mixed with RAGE primers (164 bp): sense 5′-CAGGACCAGGGAACCTACAG-3′ and antisense 5′-CATGTGTTGGGGGCTATCTT-3′; and β-actin primers (302 bp) were used as a housekeeping gene: sense 5′-GCACTCTTCCAGCCTTCCTTCC-3′ and antisense 5′-TGCTTGCTGATCCACATCTGCT-3′ (Sigma-Aldrich). .. Amplified samples ran in 2 % agarose gel electrophoresis with ethidium bromide.

Article Title: CIP2A Promotes Proliferation of Spermatogonial Progenitor Cells and Spermatogenesis in Mice
Article Snippet: Genotyping One microgram genomic DNA from mouse ear was added to the 50 µl PCR containing 75 mM Tris HCl (pH 9.0); 50 mM KCl; 2 mM MgCl2; 20 mM (NH4)2SO4; 0.2 µM of each primer, 0.2 mM dNTPs, and 2.5 U DNA polymerase (Biotools Native DNA Polymerase, BIOTOOLS B & M Labs, S.A., Spain). .. The resulting PCR products were analyzed by electrophoresis on 2% agarose gel, and the fragments were UV visualized with ethidium bromide.

In Vitro:

Article Title: Morphological and molecular identification to secure cultivar maintenance and management of self-sterile Rubus arcticus
Article Snippet: The samples used were from: field and in vitro culture collections in the Kuopio Research Garden of the University of Eastern Finland from 2009 and 2010; in vitro culture collection of Agrifood Research Finland MTT Laukaa from 2011; frozen leaf samples from the commercial nursery Biotaimi Ltd from 2006; research fields of farmers in the North-Savo region from 2009; and dried leaf samples from Agrifood Research Finland MTT Sotkamo and from Savo Vocational College Muuruvesi, both from 1995. .. The PCR reactions were performed in a 10-μL reaction mixture containing 20–30 ng template DNA, 75 m m Tris-HCl (pH 9·0), 2 m m MgCl2 , 50 m m KCl, 20 m m (NH4 )2 SO4 , 0·2 µ m of each forward and reverse primer, 200 µ m dNTP and 0·5 U DNA polymerase (Biotools, B & M Labs, S.A., Spain).

Sampling:

Article Title: Panmixia and dispersal from the Mediterranean Basin to Macaronesian Islands of a macrolichen species
Article Snippet: To identify the mating-type idiomorph of each sample, we performed multiplexed PCR reactions in a total volume of 25 μL with 0.65 μL of each MAT1-1 primer (10 μM), 0.6 μL of each MAT1-2 primer (10 μM), 0.4 μL dNTP’s (10 mM; Biotools B & M Labs, Madrid, Spain) and 0.5 μL DNA polymerase (1 U/μL, Biotools B & M Labs, Madrid, Spain) and 2.5 μL of 10–50 ng DNA. .. PCR reactions were performed with an initial denaturalization at 94 °C for 4 min, followed by 10 cycles of denaturalization step at 95 °C for 10 sec, annealing at 55 to 50 °C for 20 sec and extension at 72 °C for 30 sec, followed by 30 additional cycles with annealing temperature of 50 °C for 20 sec and an additional extension at 72 °C for 5 min. We tested if both idiomorphs were evenly distributed in the entire area and in each individual sampling locality with χ2 tests.

Concentration Assay:

Article Title: Identification of circo-like virus-Brazil genomic sequences in raw sewage from the metropolitan area of São Paulo: evidence of circulation two and three years after the first detection
Article Snippet: Viruses were concentrated from 15 L of raw sewage and 100 L of reclaimed water by filtration through Zeta Plus 60S positively charged microporous filter membranes (Cuno Inc.) followed by ultracentrifugation as previously described ( , , resulting in final concentration factors of 8,000 and 50,000 times, respectively ( . .. DNA polymerase (Biotools B & M Labs) was employed in all PCR assays (each with 35 cycles), and the annealing temperatures were calculated using the PrimerSelect program in the Lasergene package (DNASTAR Inc).

DNA Purification:

Article Title: Vitamin D receptor gene polymorphisms and susceptibility of hand osteoarthritis in Finnish women
Article Snippet: Genotyping analysis All DNA samples were extracted from lymphocytes by a DNA extraction kit (PUREGENE® DNA Purification Kit; Gentra Systems, Plymouth, MN, USA). .. Briefly, the PCR reactions were set up as follows: 50–100 ng template, 1 U DNA polymerase (Biotools; B & M Labs, SA, Madrid, Spain), 0.2 mM dNTPs, 0.5 μM each primer and 1.5 mM MgCl2 in the magnesium-free PCR buffer (Biotools; B & M Labs, SA).

CTG Assay:

Article Title: The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Article Snippet: DNA was amplified with the following primers: [5′-ACT AGG CCT CAC CTG ATA CG-3′ (sense), 5′-AGG TGT CGA TTT GCA GTG AC-3′ (antisense)] as a product with 646 bp length. .. PCR was carried out in a volume of 15 μ L containing 50 ng of genomic DNA, 0.3 μ M of each primer, 0.5 U of DNA polymerase (Biotools B & M Labs S. A., Madrid, Spain), 5 mM of MgCl2 , 10x reaction buffer MgCl2 free (Biotools B & M Labs S. A., Madrid, Spain), and 0.5 mM of deoxyribonucleoside triphosphate mix (Roche, Basel, Switzerland).

Staining:

Article Title: DNA sequence analysis suggests that cytb-nd1 PCR-RFLP may not be applicable to sandfly species identification throughout the Mediterranean region
Article Snippet: Two microliters of DNA was used in a 25-μL final volume PCR reaction, including standard reaction buffer 2 mM MgCl2 , 0.2 mM each dNTP, and 0.7 U of Thermus sp. DNA polymerase (Biotools, B & M Labs, Spain), and 15 pmol of each primer PhleF (5′-AAT AAA TTA GGA GGA GTA ATT GC-3′) and PhleR (5′-GCC TCG AWT TCG WTT ATG ATA AAT T-3′) (Sigma-Genosys). .. Amplification was performed on a 9800 Fast Thermal Cycler (Applied Biosystems), standard ramp enabled, with the following conditions: initial denaturation of 5 min at 94 °C, 40 cycles at 94 °C for 1 min, 52 °C for 1 min, and 72 °C for 1 min, and final extension at 72 °C for 10 min. All the amplified products were resolved on 2 % agarose gels stained with Pronasafe Nucleic Acid Staining (Laboratorios CONDA, Spain) and visualized under UV light.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 82
    Biotools B & M Labs enzyme biotools dna polymerase
    1% agarose gel electrophoresis of PCR products of UBP4 ′′ a : M-marker (bp) GeneRuler <t>DNA</t> Ladder Mix (Fermentas Life Sciences), lane 1: UBP4 ′′ a gene (540 bp UBP4 ′′ a length and 26 bp for restriction enzymes sequence = 566 bp). PCR reaction with <t>Biotools</t> DNA polymerase and 23 cycles.
    Enzyme Biotools Dna Polymerase, supplied by Biotools B & M Labs, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme biotools dna polymerase/product/Biotools B & M Labs
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzyme biotools dna polymerase - by Bioz Stars, 2020-03
    82/100 stars
      Buy from Supplier

    96
    Biotools B & M Labs dna polymerase
    1% agarose gel electrophoresis of PCR products of UBP4 ′′ a : M-marker (bp) GeneRuler <t>DNA</t> Ladder Mix (Fermentas Life Sciences), lane 1: UBP4 ′′ a gene (540 bp UBP4 ′′ a length and 26 bp for restriction enzymes sequence = 566 bp). PCR reaction with <t>Biotools</t> DNA polymerase and 23 cycles.
    Dna Polymerase, supplied by Biotools B & M Labs, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase/product/Biotools B & M Labs
    Average 96 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2020-03
    96/100 stars
      Buy from Supplier

    87
    Biotools B & M Labs biotools taq dna polymerase
    1% agarose gel electrophoresis of PCR products of UBP4 ′′ a : M-marker (bp) GeneRuler <t>DNA</t> Ladder Mix (Fermentas Life Sciences), lane 1: UBP4 ′′ a gene (540 bp UBP4 ′′ a length and 26 bp for restriction enzymes sequence = 566 bp). PCR reaction with <t>Biotools</t> DNA polymerase and 23 cycles.
    Biotools Taq Dna Polymerase, supplied by Biotools B & M Labs, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotools taq dna polymerase/product/Biotools B & M Labs
    Average 87 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotools taq dna polymerase - by Bioz Stars, 2020-03
    87/100 stars
      Buy from Supplier

    Image Search Results


    1% agarose gel electrophoresis of PCR products of UBP4 ′′ a : M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lane 1: UBP4 ′′ a gene (540 bp UBP4 ′′ a length and 26 bp for restriction enzymes sequence = 566 bp). PCR reaction with Biotools DNA polymerase and 23 cycles.

    Journal: BioMed Research International

    Article Title: DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

    doi: 10.1155/2015/413262

    Figure Lengend Snippet: 1% agarose gel electrophoresis of PCR products of UBP4 ′′ a : M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lane 1: UBP4 ′′ a gene (540 bp UBP4 ′′ a length and 26 bp for restriction enzymes sequence = 566 bp). PCR reaction with Biotools DNA polymerase and 23 cycles.

    Article Snippet: The PCR was performed in a 50μ L reaction volume with a buffer containing 50 mM KCl, 2 mM MgCl2, 0.02 mM of each dNTP, 75 mM Tris-HCl (pH 8.9), 20 mM (NH4)2SO4, 25 pM of each primer: Ubp4′Rev, Ubp4′For (with additional sequence for restriction enzymes: Nde I, EcoR I, and BamH I), the enzyme Biotools DNA polymerase (Biotools B & M Labs.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Marker, Sequencing

    PCR products of UBP4′: M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lanes 1–8 UBP4′ gene (276 bp UBP4′ length and 26 bp for restriction enzymes sequence = 302 bp) PCR product: lanes 1, 2: PCR reaction with Biotools DNA polymerase and 23 cycles, lanes 3, 4: PCR reaction with Biotools DNA polymerase and 29 cycles, lanes 5, 6: PCR reaction with Biotools DNA polymerase and 29 cycles, and lanes 7, 8: PCR reaction with Taq DNA polymerase and 29 cycles.

    Journal: BioMed Research International

    Article Title: DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

    doi: 10.1155/2015/413262

    Figure Lengend Snippet: PCR products of UBP4′: M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lanes 1–8 UBP4′ gene (276 bp UBP4′ length and 26 bp for restriction enzymes sequence = 302 bp) PCR product: lanes 1, 2: PCR reaction with Biotools DNA polymerase and 23 cycles, lanes 3, 4: PCR reaction with Biotools DNA polymerase and 29 cycles, lanes 5, 6: PCR reaction with Biotools DNA polymerase and 29 cycles, and lanes 7, 8: PCR reaction with Taq DNA polymerase and 29 cycles.

    Article Snippet: The PCR was performed in a 50μ L reaction volume with a buffer containing 50 mM KCl, 2 mM MgCl2, 0.02 mM of each dNTP, 75 mM Tris-HCl (pH 8.9), 20 mM (NH4)2SO4, 25 pM of each primer: Ubp4′Rev, Ubp4′For (with additional sequence for restriction enzymes: Nde I, EcoR I, and BamH I), the enzyme Biotools DNA polymerase (Biotools B & M Labs.

    Techniques: Polymerase Chain Reaction, Marker, Sequencing

    Analysis of the products was performed by 8% acrylamide gel electrophoresis. PCR products of UBP4 ′′ b : M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lane 1: UBP4 ′′ b gene (543 bp UBP4 ′′ b length and 26 bp for restriction enzymes sequence = 569 bp). PCR reaction with Biotools DNA polymerase and 23 cycles.

    Journal: BioMed Research International

    Article Title: DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

    doi: 10.1155/2015/413262

    Figure Lengend Snippet: Analysis of the products was performed by 8% acrylamide gel electrophoresis. PCR products of UBP4 ′′ b : M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lane 1: UBP4 ′′ b gene (543 bp UBP4 ′′ b length and 26 bp for restriction enzymes sequence = 569 bp). PCR reaction with Biotools DNA polymerase and 23 cycles.

    Article Snippet: The PCR was performed in a 50μ L reaction volume with a buffer containing 50 mM KCl, 2 mM MgCl2, 0.02 mM of each dNTP, 75 mM Tris-HCl (pH 8.9), 20 mM (NH4)2SO4, 25 pM of each primer: Ubp4′Rev, Ubp4′For (with additional sequence for restriction enzymes: Nde I, EcoR I, and BamH I), the enzyme Biotools DNA polymerase (Biotools B & M Labs.

    Techniques: Acrylamide Gel Assay, Electrophoresis, Polymerase Chain Reaction, Marker, Sequencing