dna manipulation  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dna manipulation
    Dna Manipulation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna manipulation/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna manipulation - by Bioz Stars, 2020-05
    91/100 stars

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    Related Articles

    Clone Assay:

    Article Title: HBX-Mediated Migration of HBV-Replicating HepG2 Cells: Insights on Development of Hepatocellular Carcinoma
    Article Snippet: .. Plasmids Construction and DNA Manipulation The replicative HBV genome comprising a 3.5kb linear fragment was constructed as described [ ] in mammalian expression vector pcDNA3.1 (Invitrogen, USA), using a two step cloning strategy. .. Wild-type replicative HBV genome was used as template to generate proline to alanine mutation by site-directed mutagenesis as described in [ ].

    Amplification:

    Article Title: mgr, a Novel Global Regulator in Staphylococcus aureus
    Article Snippet: .. The enzymes used in DNA manipulation were obtained from GIBCO-BRL (Gaithersburg, Md.), New England Biolabs, Inc. (Beverly, Mass.), or Promega Corp. PCR amplification was carried out with the Advantage cDNA PCR kit (Clontech, Palo Alto, Calif.). ..

    Article Title: Engineering biosynthesis of the anticancer alkaloid noscapine in yeast
    Article Snippet: .. For DNA manipulation and amplification, we used chemically competent or electrocompetent E. coli strains TOP10 and ccdB Survival 2 T1R purchased from Life Technologies. .. E. coli strains were grown at 37 °C in lysogeny broth medium obtained from Fisher Scientific supplemented with 100 mg ml−1 ampicillin (EMD Chemicals) or 50 mg ml−1 kanamycin (Fisher Scientific) for plasmid maintenance.

    Construct:

    Article Title: HBX-Mediated Migration of HBV-Replicating HepG2 Cells: Insights on Development of Hepatocellular Carcinoma
    Article Snippet: .. Plasmids Construction and DNA Manipulation The replicative HBV genome comprising a 3.5kb linear fragment was constructed as described [ ] in mammalian expression vector pcDNA3.1 (Invitrogen, USA), using a two step cloning strategy. .. Wild-type replicative HBV genome was used as template to generate proline to alanine mutation by site-directed mutagenesis as described in [ ].

    Expressing:

    Article Title: HBX-Mediated Migration of HBV-Replicating HepG2 Cells: Insights on Development of Hepatocellular Carcinoma
    Article Snippet: .. Plasmids Construction and DNA Manipulation The replicative HBV genome comprising a 3.5kb linear fragment was constructed as described [ ] in mammalian expression vector pcDNA3.1 (Invitrogen, USA), using a two step cloning strategy. .. Wild-type replicative HBV genome was used as template to generate proline to alanine mutation by site-directed mutagenesis as described in [ ].

    Polymerase Chain Reaction:

    Article Title: mgr, a Novel Global Regulator in Staphylococcus aureus
    Article Snippet: .. The enzymes used in DNA manipulation were obtained from GIBCO-BRL (Gaithersburg, Md.), New England Biolabs, Inc. (Beverly, Mass.), or Promega Corp. PCR amplification was carried out with the Advantage cDNA PCR kit (Clontech, Palo Alto, Calif.). ..

    Plasmid Preparation:

    Article Title: p19-Targeting ILP Protein Blockers of IL-23/Th-17 Pro-Inflammatory Axis Displayed on Engineered Bacteria of Food Origin
    Article Snippet: .. DNA Manipulation and Plasmid Construction Restriction enzymes and T4 DNA ligase were purchased from Fermentas or New England Biolabs. .. PCR amplifications were performed with Taq polymerase (Fermentas, Waltham, MA, USA) or Phusion Hot Start polymerase (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer´s protocols.

    Article Title: HBX-Mediated Migration of HBV-Replicating HepG2 Cells: Insights on Development of Hepatocellular Carcinoma
    Article Snippet: .. Plasmids Construction and DNA Manipulation The replicative HBV genome comprising a 3.5kb linear fragment was constructed as described [ ] in mammalian expression vector pcDNA3.1 (Invitrogen, USA), using a two step cloning strategy. .. Wild-type replicative HBV genome was used as template to generate proline to alanine mutation by site-directed mutagenesis as described in [ ].

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  • 93
    Thermo Fisher dna manipulations
    Dna Manipulations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna manipulations/product/Thermo Fisher
    Average 93 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    dna manipulations - by Bioz Stars, 2020-05
    93/100 stars
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