dna extraction  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Qiagen dna extraction
    Dna Extraction, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna extraction/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna extraction - by Bioz Stars, 2021-04
    86/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
    Article Snippet: Thereafter, 16S rDNA PCR products were visualized using electrophoresis on 2% agarose gel and analysed by DGGE (Figures and ). .. Lanes exhibiting 16S rDNA PCR products obtained from easyMag® DNA were blank, while PCR products obtained from DNA extracted by Qiagen were clearly visible. ..

    Article Title: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
    Article Snippet: .. However, the DGGE gel lanes representing 16S rDNA PCR products of the DNA extracted by Qiagen revealed clearly more visible bands in comparison with DGGE gel lanes representing 16S rDNA PCR products derived from DNA extracted by easyMag®. .. The reason why Qiagen extracted DNA is of better quality may be found in the method used to extract fecal DNA.

    DNA Extraction:

    Article Title: Invasion of sorghum in the Americas by a new sugarcane aphid (Melanaphis sacchari) superclone
    Article Snippet: Destructive DNA extraction was performed according to the manufacturer’s protocol with modifications; the aphid was ground in a 1.5 mL Eppendorf tube with a TissueLyser II, Qiagen using glass beads for 3 min at 30 Hz, in 20 μL of the ready-to-use proteinase K solution of the Qiagen DNeasy Blood & Tissue Kits. .. The non-destructive DNA extraction allowed specimens to be analyzed for both microsatellite and morphometrics by slide mounting (‘lame’ mentioned in , column field code). .. Non-destructive DNA extraction was performed according to the manufacturer’s protocol and the insect body was retrieved from the first elution column for slide mounting (potassium chloride treatment, followed by a chloral hydrate plus phenol treatment).

    Article Title: Invasion of sorghum in the Americas by a new sugarcane aphid (Melanaphis sacchari) superclone
    Article Snippet: The non-destructive DNA extraction allowed specimens to be analyzed for both microsatellite and morphometrics by slide mounting (‘lame’ mentioned in , column field code). .. Non-destructive DNA extraction was performed according to the manufacturer’s protocol and the insect body was retrieved from the first elution column for slide mounting (potassium chloride treatment, followed by a chloral hydrate plus phenol treatment). ..

    Denaturing Gradient Gel Electrophoresis:

    Article Title: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
    Article Snippet: .. However, the DGGE gel lanes representing 16S rDNA PCR products of the DNA extracted by Qiagen revealed clearly more visible bands in comparison with DGGE gel lanes representing 16S rDNA PCR products derived from DNA extracted by easyMag®. .. The reason why Qiagen extracted DNA is of better quality may be found in the method used to extract fecal DNA.

    Article Title: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
    Article Snippet: As Figure shows, 16S rDNA PCR products obtained using easyMag® revealed more bands and showed bands with higher densities in the lanes where the DNA was diluted 10 and 15 times. .. Additionally, almost identical bands appear for each faecal sample in DGGE gel lane in both DNA extracts; diluted easyMag® DNA extracts and Qiagen DNA extracts (Figures and ). .. However, the lanes in DGGE gels representing DNA extracted by Qiagen show brightest bands in comparison to DNA extracted by easyMag®.

    Derivative Assay:

    Article Title: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
    Article Snippet: .. However, the DGGE gel lanes representing 16S rDNA PCR products of the DNA extracted by Qiagen revealed clearly more visible bands in comparison with DGGE gel lanes representing 16S rDNA PCR products derived from DNA extracted by easyMag®. .. The reason why Qiagen extracted DNA is of better quality may be found in the method used to extract fecal DNA.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Qiagen hbv dna extraction
    Between-run precision of COBAS Amplicor <t>HBV</t> Monitor assay. Fifty-two clinical samples were tested undiluted in duplicate in the Amplicor assay on separate days. The means and standard deviations of log HBV <t>DNA</t> copies/milliliter of the duplicate values
    Hbv Dna Extraction, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbv dna extraction/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbv dna extraction - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Qiagen extract dna
    Southern blot analysis of tail <t>DNA</t> from F 2 animals. Five micrograms of genomic DNA was digested with EcoRI or EcoRV, separated by agarose gel electrophoresis, and analyzed by Southern blot hybridization with a probe specific for sequences in the transgenic construct. Shown are wild-type FVB/Nj animals (wt), a <t>HuPAR-2</t> transgene-negative F 2 littermate, and a HuPAR-2 transgene-positive F 2 animal. EcoRI cuts twice within the transgene, and EcoRV cuts once; the relationship to the probe is shown below the image. The experiment was repeated twice on different littermates with similar results.
    Extract Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extract dna/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    extract dna - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Qiagen qiagen dna extracts
    Gel electrophoresis of nested PCR amplification products obtained from <t>DNA</t> extracted from horse pus and blood samples. DNA preparations were amplified with primers P3/2R8 (first round) and then diluted 1 in 10 and subjected to a second round of PCR amplification with PCR primers F5/2R5 to generate ITS gene products (514 bp) indicative of the presence of Histoplasma DNA. All amplification products were subsequently sequenced to confirm > 97% identity and the closest match to Histoplasma capsulatus ITS region DNA. Lanes 1 to 4, negative controls (DNA extracts from S. cerevisiae , E. coli , pus from a horse in the UK, and DNA- and RNA-free water, respectively); lane 5, H. capsulatum var. capsulatum control DNA; lane 6, H. capsulatum var. farciminosum control DNA; lanes 7 to 25, PCR amplicons of <t>Qiagen</t> DNA extracts of blood and pus from horses with suspected EZL (lanes 7 to 23, DNA extracts from pus, lanes 24 and 25, DNA extracts from blood); lanes MW, molecular weight markers).
    Qiagen Dna Extracts, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen dna extracts/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiagen dna extracts - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Qiagen genomic dna extraction
    <t>PCR-RFLP</t> method to identify A. cruentus . a. A single 278-bp fragment was amplified from three cultivated grain species of Amaranthus using primers specific for the SBE locus (see Materials and Methods for details). Markers represent a 100-pb <t>DNA</t> ladder. b. Schematic and result of PCR-RFLP for identifying A. cruentus using intron 11 of the SBE locus. Restriction profiles of PCR amplification of intron 11 of SBE followed by digestion with Mse I. Restriction enzyme cleavage site is shown in bold, and one-base substitution, T-C polymorphism is underlined. Markers represent a100-bp DNA ladder.
    Genomic Dna Extraction, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna extraction/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genomic dna extraction - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Between-run precision of COBAS Amplicor HBV Monitor assay. Fifty-two clinical samples were tested undiluted in duplicate in the Amplicor assay on separate days. The means and standard deviations of log HBV DNA copies/milliliter of the duplicate values

    Journal: Journal of Clinical Microbiology

    Article Title: Evaluation of the COBAS Amplicor HBV Monitor Assay and Comparison with the Ultrasensitive HBV Hybrid Capture 2 Assay for Quantification of Hepatitis B Virus DNA

    doi: 10.1128/JCM.43.2.596-603.2005

    Figure Lengend Snippet: Between-run precision of COBAS Amplicor HBV Monitor assay. Fifty-two clinical samples were tested undiluted in duplicate in the Amplicor assay on separate days. The means and standard deviations of log HBV DNA copies/milliliter of the duplicate values

    Article Snippet: HBV DNA extraction from clinical serum and plasma samples was achieved by using QIAamp DNA Blood Mini kit protease (QIAGEN Inc.), and buffers (AW1 and AW2) were reconstituted as per the manufacturer's instructions.

    Techniques:

    COBAS Amplicor HBV Monitor assay linearity. Eight samples were serially diluted in HBV-seronegative NHS and tested by the Amplicor assay in triplicate on the same day. The means of triplicate values were calculated and plotted as log HBV DNA copies/milliliter.

    Journal: Journal of Clinical Microbiology

    Article Title: Evaluation of the COBAS Amplicor HBV Monitor Assay and Comparison with the Ultrasensitive HBV Hybrid Capture 2 Assay for Quantification of Hepatitis B Virus DNA

    doi: 10.1128/JCM.43.2.596-603.2005

    Figure Lengend Snippet: COBAS Amplicor HBV Monitor assay linearity. Eight samples were serially diluted in HBV-seronegative NHS and tested by the Amplicor assay in triplicate on the same day. The means of triplicate values were calculated and plotted as log HBV DNA copies/milliliter.

    Article Snippet: HBV DNA extraction from clinical serum and plasma samples was achieved by using QIAamp DNA Blood Mini kit protease (QIAGEN Inc.), and buffers (AW1 and AW2) were reconstituted as per the manufacturer's instructions.

    Techniques:

    Deming regression comparison of Digene HC2 assay and COBAS Amplicor HBV Monitor assay. One-hundred samples previously tested using the HC2 assay were tested undiluted in the Amplicor assay. Samples with results greater than 200,000 HBV DNA copies/ml were

    Journal: Journal of Clinical Microbiology

    Article Title: Evaluation of the COBAS Amplicor HBV Monitor Assay and Comparison with the Ultrasensitive HBV Hybrid Capture 2 Assay for Quantification of Hepatitis B Virus DNA

    doi: 10.1128/JCM.43.2.596-603.2005

    Figure Lengend Snippet: Deming regression comparison of Digene HC2 assay and COBAS Amplicor HBV Monitor assay. One-hundred samples previously tested using the HC2 assay were tested undiluted in the Amplicor assay. Samples with results greater than 200,000 HBV DNA copies/ml were

    Article Snippet: HBV DNA extraction from clinical serum and plasma samples was achieved by using QIAamp DNA Blood Mini kit protease (QIAGEN Inc.), and buffers (AW1 and AW2) were reconstituted as per the manufacturer's instructions.

    Techniques: HC2 Assay

    Southern blot analysis of tail DNA from F 2 animals. Five micrograms of genomic DNA was digested with EcoRI or EcoRV, separated by agarose gel electrophoresis, and analyzed by Southern blot hybridization with a probe specific for sequences in the transgenic construct. Shown are wild-type FVB/Nj animals (wt), a HuPAR-2 transgene-negative F 2 littermate, and a HuPAR-2 transgene-positive F 2 animal. EcoRI cuts twice within the transgene, and EcoRV cuts once; the relationship to the probe is shown below the image. The experiment was repeated twice on different littermates with similar results.

    Journal: Journal of Virology

    Article Title: Mice Transgenic for a Human Porcine Endogenous Retrovirus Receptor Are Susceptible to Productive Viral Infection †

    doi: 10.1128/JVI.80.7.3135-3146.2006

    Figure Lengend Snippet: Southern blot analysis of tail DNA from F 2 animals. Five micrograms of genomic DNA was digested with EcoRI or EcoRV, separated by agarose gel electrophoresis, and analyzed by Southern blot hybridization with a probe specific for sequences in the transgenic construct. Shown are wild-type FVB/Nj animals (wt), a HuPAR-2 transgene-negative F 2 littermate, and a HuPAR-2 transgene-positive F 2 animal. EcoRI cuts twice within the transgene, and EcoRV cuts once; the relationship to the probe is shown below the image. The experiment was repeated twice on different littermates with similar results.

    Article Snippet: Cultured primary kidney cells from HuPAR-2 transgenics and HuPAR-2-negative littermates (105 cells/sample) were used to extract DNA with the DNAeasy kit (QIAGEN).

    Techniques: Southern Blot, Agarose Gel Electrophoresis, Hybridization, Transgenic Assay, Construct

    Gel electrophoresis of nested PCR amplification products obtained from DNA extracted from horse pus and blood samples. DNA preparations were amplified with primers P3/2R8 (first round) and then diluted 1 in 10 and subjected to a second round of PCR amplification with PCR primers F5/2R5 to generate ITS gene products (514 bp) indicative of the presence of Histoplasma DNA. All amplification products were subsequently sequenced to confirm > 97% identity and the closest match to Histoplasma capsulatus ITS region DNA. Lanes 1 to 4, negative controls (DNA extracts from S. cerevisiae , E. coli , pus from a horse in the UK, and DNA- and RNA-free water, respectively); lane 5, H. capsulatum var. capsulatum control DNA; lane 6, H. capsulatum var. farciminosum control DNA; lanes 7 to 25, PCR amplicons of Qiagen DNA extracts of blood and pus from horses with suspected EZL (lanes 7 to 23, DNA extracts from pus, lanes 24 and 25, DNA extracts from blood); lanes MW, molecular weight markers).

    Journal: Journal of Clinical Microbiology

    Article Title: Development and Evaluation of a Molecular Diagnostic Method for Rapid Detection of Histoplasma capsulatum var. farciminosum, the Causative Agent of Epizootic Lymphangitis, in Equine Clinical Samples

    doi: 10.1128/JCM.00896-16

    Figure Lengend Snippet: Gel electrophoresis of nested PCR amplification products obtained from DNA extracted from horse pus and blood samples. DNA preparations were amplified with primers P3/2R8 (first round) and then diluted 1 in 10 and subjected to a second round of PCR amplification with PCR primers F5/2R5 to generate ITS gene products (514 bp) indicative of the presence of Histoplasma DNA. All amplification products were subsequently sequenced to confirm > 97% identity and the closest match to Histoplasma capsulatus ITS region DNA. Lanes 1 to 4, negative controls (DNA extracts from S. cerevisiae , E. coli , pus from a horse in the UK, and DNA- and RNA-free water, respectively); lane 5, H. capsulatum var. capsulatum control DNA; lane 6, H. capsulatum var. farciminosum control DNA; lanes 7 to 25, PCR amplicons of Qiagen DNA extracts of blood and pus from horses with suspected EZL (lanes 7 to 23, DNA extracts from pus, lanes 24 and 25, DNA extracts from blood); lanes MW, molecular weight markers).

    Article Snippet: In total, 25 of the 27 EZL case horses tested positive on the basis of analysis of Qiagen DNA extracts from pus, and 17 of the 27 (63%) EZL case horses tested positive on the basis of analysis of Qiagen DNA extracts from blood samples; example results are presented in .

    Techniques: Nucleic Acid Electrophoresis, Nested PCR, Amplification, Polymerase Chain Reaction, Molecular Weight

    PCR-RFLP method to identify A. cruentus . a. A single 278-bp fragment was amplified from three cultivated grain species of Amaranthus using primers specific for the SBE locus (see Materials and Methods for details). Markers represent a 100-pb DNA ladder. b. Schematic and result of PCR-RFLP for identifying A. cruentus using intron 11 of the SBE locus. Restriction profiles of PCR amplification of intron 11 of SBE followed by digestion with Mse I. Restriction enzyme cleavage site is shown in bold, and one-base substitution, T-C polymorphism is underlined. Markers represent a100-bp DNA ladder.

    Journal: Breeding Science

    Article Title: A rapid and reliable PCR-restriction fragment length polymorphism (RFLP) marker for the identification of Amaranthus cruentus species

    doi: 10.1270/jsbbs.64.422

    Figure Lengend Snippet: PCR-RFLP method to identify A. cruentus . a. A single 278-bp fragment was amplified from three cultivated grain species of Amaranthus using primers specific for the SBE locus (see Materials and Methods for details). Markers represent a 100-pb DNA ladder. b. Schematic and result of PCR-RFLP for identifying A. cruentus using intron 11 of the SBE locus. Restriction profiles of PCR amplification of intron 11 of SBE followed by digestion with Mse I. Restriction enzyme cleavage site is shown in bold, and one-base substitution, T-C polymorphism is underlined. Markers represent a100-bp DNA ladder.

    Article Snippet: Genomic DNA extraction and PCR amplification Genomic DNA was extracted from young leaves using the CTAB method ( ) or the DNeasy Plant Mini kit (Qiagen, Hilden, Germany).

    Techniques: Polymerase Chain Reaction, Amplification