dna exonuclease digestions  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Exonuclease I 20 U µL
    Description:
    Thermo Scientific Exonuclease I ExoI degrades single stranded DNA in a 3 →5 direction It releases deoxyribonucleoside 5 monophosphates in a stepwise manner and leaves 5 terminal dinucleotides intact It does not cleave DNA strands with terminal 3 OH groups blocked by phosphoryl or acetyl groups Highlights• Active in PCR buffersApplications• Primer removal from PCR mixtures • prior to PCR product sequencing• for one tube megaprimer PCR mutagenesis• Removal of single stranded DNA containing a 3 hydroxyl terminus from nucleic acid mixtures• Assay for the presence of single stranded DNA with a 3 hydroxyl terminusNoteThe enzyme is not suitable for removing 3 overhangs of dsDNA
    Catalog Number:
    en0581
    Price:
    None
    Applications:
    Cloning|PCR Cloning|Mutagenesis
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher dna exonuclease digestions
    Thermo Scientific Exonuclease I ExoI degrades single stranded DNA in a 3 →5 direction It releases deoxyribonucleoside 5 monophosphates in a stepwise manner and leaves 5 terminal dinucleotides intact It does not cleave DNA strands with terminal 3 OH groups blocked by phosphoryl or acetyl groups Highlights• Active in PCR buffersApplications• Primer removal from PCR mixtures • prior to PCR product sequencing• for one tube megaprimer PCR mutagenesis• Removal of single stranded DNA containing a 3 hydroxyl terminus from nucleic acid mixtures• Assay for the presence of single stranded DNA with a 3 hydroxyl terminusNoteThe enzyme is not suitable for removing 3 overhangs of dsDNA
    https://www.bioz.com/result/dna exonuclease digestions/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna exonuclease digestions - by Bioz Stars, 2020-07
    99/100 stars

    Related Products / Commonly Used Together

    bigdye protocol

    Images

    Related Articles

    Hydrolysis Assay:

    Article Title: An exonuclease I hydrolysis assay for evaluating G-quadruplex stabilization by small molecules
    Article Snippet: .. Exonuclease I hydrolysis assay Oligonucleotides were labeled at the 5′ end with [γ-32 P]ATP using T4 polynucleotide kinase (Fermentas, Lithuania). ..

    Southern Blot:

    Article Title: Defects in coding joint formation in vivo in developing ATM-deficient B and T lymphocytes
    Article Snippet: .. For exonuclease V digestion, 15 μg of thymocyte DNA was treated with increasing concentrations of exonuclease V, using the manufacturer's buffer conditions (USB Corporation), for 1 h at 37°C before restriction enzyme digestion and Southern blot analyses. .. For mung bean nuclease assays, 15 μg of thymocyte DNA was digested with 12.5 U of mung bean nuclease (New England Biolabs, Inc.) in 200 μl using the manufacturer's buffer supplemented with 0.4 mM ZnSO4 at 25°C for 1 h before treatment with exonuclease V, as described in this section.

    Ethanol Precipitation:

    Article Title: Transcription-induced formation of extrachromosomal DNA during yeast ageing
    Article Snippet: .. A total of 6 μl 10 mM ATP, 1 μl 10x CutSmart, 1 μl Exonuclease V, (RecBCD, M0345) and 1 μl Exonuclease I (M0293) was added to each digest and incubated over night at 37°C before extraction with phenol chloroform and ethanol precipitation in the presence of 1 μl GlycoBlue (AM9516 ThermoFisher Scientific, UK). .. DNA pellets were dissolved in 45 μl 0.1x TE, then 6 μl 10x CutSmart, 6 μl 10 mM ATP, 1 μl Exonuclease V, 1 μl Exonuclease I, and 1 μl same restriction enzyme as previous day were added and samples again incubated over night at 37°C before extraction with phenol chloroform and ethanol precipitation.

    Article Title: Mutations in STN1 cause Coats plus syndrome and are associated with genomic and telomere defects
    Article Snippet: .. Exonuclease I treatment was performed overnight at 37°C with 200 U exonuclease I (Thermo Fisher Scientific) per 5 µg DNA in 500 µl, followed by ethanol precipitation. ..

    Labeling:

    Article Title: An exonuclease I hydrolysis assay for evaluating G-quadruplex stabilization by small molecules
    Article Snippet: .. Exonuclease I hydrolysis assay Oligonucleotides were labeled at the 5′ end with [γ-32 P]ATP using T4 polynucleotide kinase (Fermentas, Lithuania). ..

    SYBR Green Assay:

    Article Title: Terminal hairpin in oligonucleotide dominantly prioritizes intramolecular cyclization by T4 ligase over intermolecular polymerization: an exclusive methodology for producing ssDNA rings
    Article Snippet: .. T4 DNA ligase, Exonuclease I and SYBR Green II were also obtained from Thermo Scientific (Pittsburgh, PA, USA). .. EvaGreen with the concentration of 20× was purchased from Biotium (Fremont, CA, USA).

    Concentration Assay:

    Article Title: MHC Multimer-Guided and Cell Culture-Independent Isolation of Functional T Cell Receptors from Single Cells Facilitates TCR Identification for Immunotherapy
    Article Snippet: .. For primer exonuclease I digest, reaction volume was filled up to 1 µl resulting in a final concentration of 67 mM glycine-KOH (pH 9.5), 6.7 mM MgCl2 , 10 mM DTT and 1 U/ µl Exonuclease I (Fermentas). .. Exonuclease-I digest was performed for 30 min at 37°C and enzyme was inactivated for 20 min at 70°C on an AmpliSpeed PCR cycler.

    Incubation:

    Article Title: Transcription-induced formation of extrachromosomal DNA during yeast ageing
    Article Snippet: .. A total of 6 μl 10 mM ATP, 1 μl 10x CutSmart, 1 μl Exonuclease V, (RecBCD, M0345) and 1 μl Exonuclease I (M0293) was added to each digest and incubated over night at 37°C before extraction with phenol chloroform and ethanol precipitation in the presence of 1 μl GlycoBlue (AM9516 ThermoFisher Scientific, UK). .. DNA pellets were dissolved in 45 μl 0.1x TE, then 6 μl 10x CutSmart, 6 μl 10 mM ATP, 1 μl Exonuclease V, 1 μl Exonuclease I, and 1 μl same restriction enzyme as previous day were added and samples again incubated over night at 37°C before extraction with phenol chloroform and ethanol precipitation.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Thermo Fisher phire plant direct pcr kit
    Phire Plant Direct Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phire plant direct pcr kit/product/Thermo Fisher
    Average 95 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    phire plant direct pcr kit - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    Image Search Results