dmem medium  (atcc)


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    Structured Review

    atcc dmem medium
    Impact of peptides A9 and A14 on ACE2 levels in cells. ( A , B ) Immunoblots showing the changes in ACE2 levels of HEK293T cells after treatment with A9 and A14 at the different tested concentrations and ( C , D ) impact of A9 and A14 on cellular levels of ACE2 using an activity assay Kit (ab273297, Abcam, Toronto, ON). HEK293T cells were grown in <t>DMEM</t> complete media containing <t>10%</t> <t>FBS</t> with antibiotics. The cells were treated with vehicle (nuclease free water) or A9 or A14 at different concentrations (25, 50, and 100 µM) for 24 h. Thereafter, protein was extracted using RIPA buffer and was stored at −20 °C till further analysis. For immunoblotting, the results are expressed as change in ACE2 fold change with respect vehicle control (nuclease free water). For ACE2 activity assay, the results were expressed as nmol of ACE2 per µL of cell extract. Data expressed as mean ± SEM of n = 4–6. p values were determined by Analysis by one-way ANOVA followed by Bonferroni’s post hoc test for vehicle. * p
    Dmem Medium, supplied by atcc, used in various techniques. Bioz Stars score: 86/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem medium/product/atcc
    Average 86 stars, based on 40 article reviews
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    dmem medium - by Bioz Stars, 2022-11
    86/100 stars

    Images

    1) Product Images from "Structurally Modified Bioactive Peptide Inhibits SARS-CoV-2 Lentiviral Particles Expression"

    Article Title: Structurally Modified Bioactive Peptide Inhibits SARS-CoV-2 Lentiviral Particles Expression

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics14102045

    Impact of peptides A9 and A14 on ACE2 levels in cells. ( A , B ) Immunoblots showing the changes in ACE2 levels of HEK293T cells after treatment with A9 and A14 at the different tested concentrations and ( C , D ) impact of A9 and A14 on cellular levels of ACE2 using an activity assay Kit (ab273297, Abcam, Toronto, ON). HEK293T cells were grown in DMEM complete media containing 10% FBS with antibiotics. The cells were treated with vehicle (nuclease free water) or A9 or A14 at different concentrations (25, 50, and 100 µM) for 24 h. Thereafter, protein was extracted using RIPA buffer and was stored at −20 °C till further analysis. For immunoblotting, the results are expressed as change in ACE2 fold change with respect vehicle control (nuclease free water). For ACE2 activity assay, the results were expressed as nmol of ACE2 per µL of cell extract. Data expressed as mean ± SEM of n = 4–6. p values were determined by Analysis by one-way ANOVA followed by Bonferroni’s post hoc test for vehicle. * p
    Figure Legend Snippet: Impact of peptides A9 and A14 on ACE2 levels in cells. ( A , B ) Immunoblots showing the changes in ACE2 levels of HEK293T cells after treatment with A9 and A14 at the different tested concentrations and ( C , D ) impact of A9 and A14 on cellular levels of ACE2 using an activity assay Kit (ab273297, Abcam, Toronto, ON). HEK293T cells were grown in DMEM complete media containing 10% FBS with antibiotics. The cells were treated with vehicle (nuclease free water) or A9 or A14 at different concentrations (25, 50, and 100 µM) for 24 h. Thereafter, protein was extracted using RIPA buffer and was stored at −20 °C till further analysis. For immunoblotting, the results are expressed as change in ACE2 fold change with respect vehicle control (nuclease free water). For ACE2 activity assay, the results were expressed as nmol of ACE2 per µL of cell extract. Data expressed as mean ± SEM of n = 4–6. p values were determined by Analysis by one-way ANOVA followed by Bonferroni’s post hoc test for vehicle. * p

    Techniques Used: Western Blot, Activity Assay

    Impact of peptides A9 and A14 on TMPRSS2 and Furin levels in cells. Immunoblots showing the changes in ( A , B ) TMPRSS2 and ( C , D ) furin levels of HEK293T cells after treatment with A9 and A14 at the different tested concentrations. HEK293T cells were grown in DMEM complete media containing 10% FBS with antibiotics. The cells were treated with vehicle (nuclease free water) or A9 or A14 at different concentrations (25, 50, and 100 µM) for 24 h. Thereafter, protein was extracted using RIPA buffer and was stored at −20 °C till further analysis. The results are expressed as change in TMPRSS2 and furin fold change with respect vehicle control (nuclease free water). Data expressed as mean ± SEM of n = 3. p values were determined by Analysis by one-way ANOVA followed by Bonferroni’s post hoc test for vehicle. ** p
    Figure Legend Snippet: Impact of peptides A9 and A14 on TMPRSS2 and Furin levels in cells. Immunoblots showing the changes in ( A , B ) TMPRSS2 and ( C , D ) furin levels of HEK293T cells after treatment with A9 and A14 at the different tested concentrations. HEK293T cells were grown in DMEM complete media containing 10% FBS with antibiotics. The cells were treated with vehicle (nuclease free water) or A9 or A14 at different concentrations (25, 50, and 100 µM) for 24 h. Thereafter, protein was extracted using RIPA buffer and was stored at −20 °C till further analysis. The results are expressed as change in TMPRSS2 and furin fold change with respect vehicle control (nuclease free water). Data expressed as mean ± SEM of n = 3. p values were determined by Analysis by one-way ANOVA followed by Bonferroni’s post hoc test for vehicle. ** p

    Techniques Used: Western Blot

    Impact of peptides A9 and A14 on over expressing SARS-CoV-2 S (spike) protein in HEK293T cells. Immunoblots showing the changes in ( A ) SARS-CoV-2 S (spike) and ( B ) OXPHOS levels of HEK293T cells after treatment with A9 and A14 at the tested concentration. HEK293T cells were grown in DMEM complete media containing 10% FBS with antibiotics. The SARS-CoV-2 S (spike) protein was overexpressed in cells. These cells were treated with vehicle (nuclease free water) or A9 or A14 at 50 µM for 24 h. Thereafter, protein was extracted using RIPA buffer and was stored at −20 °C till further analysis. These experiments were repeated thrice independently. µM: micromolar.
    Figure Legend Snippet: Impact of peptides A9 and A14 on over expressing SARS-CoV-2 S (spike) protein in HEK293T cells. Immunoblots showing the changes in ( A ) SARS-CoV-2 S (spike) and ( B ) OXPHOS levels of HEK293T cells after treatment with A9 and A14 at the tested concentration. HEK293T cells were grown in DMEM complete media containing 10% FBS with antibiotics. The SARS-CoV-2 S (spike) protein was overexpressed in cells. These cells were treated with vehicle (nuclease free water) or A9 or A14 at 50 µM for 24 h. Thereafter, protein was extracted using RIPA buffer and was stored at −20 °C till further analysis. These experiments were repeated thrice independently. µM: micromolar.

    Techniques Used: Expressing, Western Blot, Concentration Assay

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