dmem f12 hepes gibco  (Thermo Fisher)

Journal: iScience

Article Title: Pancreatic cancer organoid-screening captures personalized sensitivity and chemoresistance suppression upon cytochrome P450 3A5-targeted inhibition

doi: 10.1016/j.isci.2024.110289

Figure Lengend Snippet:

Article Snippet: The organoid medium formulation consisted of Gibco Advanced DMEM/F12 (cat. no. 12634028) supplemented with 10 mmol/L HEPES (cat. no. 15630080), 1X GlutaMAX (cat. no. 35050061), 1X B27 supplement (cat. no. 17504044), and 1X penicillin/streptomycin (cat. no. 15140122) (all from Thermo Fisher Scientific); 500 nmol/L A 83-01, 50 ng/mL EGF, 100 ng/mL noggin, 100 ng/mL FGF-10, 10 nmol/L gastrin, 1.25 mmol/L N-acetylcysteine, 10 mmol/L nicotinamide (all from the ATCC Organoid Growth Kit, cat. no. ACS-7101); 50% Wnt-3A (ATCC, cat. no CRL-2647) and 10% R-spondin (Trevigen, cat. no. 3710-001-01) conditioned medium; and 10 μmol/L ROCK Inhibitor Y-27632 (Sigma-Aldrich, cat. no. 688000).

Techniques: Recombinant, Membrane, Saline, Electron Microscopy, Staining, Lysis, Extraction, Protease Inhibitor, Sequencing, Modification, Bicinchoninic Acid Protein Assay, Software, High Content Screening