dl dithiothreitol  (Millipore)


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  • 99
    Name:
    DL Dithiothreitol
    Description:

    Catalog Number:
    d3801
    Price:
    None
    Applications:
    Additive for ethyl acetate.
    Buy from Supplier


    Structured Review

    Millipore dl dithiothreitol
    DL Dithiothreitol

    https://www.bioz.com/result/dl dithiothreitol/product/Millipore
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    dl dithiothreitol - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Effects of Transglutaminase on the Protein Network and In Vitro Starch Digestibility of Asian Wheat Noodles"

    Article Title: Effects of Transglutaminase on the Protein Network and In Vitro Starch Digestibility of Asian Wheat Noodles

    Journal: Foods

    doi: 10.3390/foods8120607

    Protein solubility of ( A ) raw and ( B ) cooked noodles with 0%, 0.5%, 1.0%, 1.5% and 2.0% transglutaminase in extracting reagents phosphate buffer (PB; 0.1 M; pH 6.8), 8 M urea in PB (PB + Urea), 0.05 M dithiothreitol in PB (PB + DTT) and 0.05 M sodium dodecyl sulphate in PB (PB + SDS); * indicates significant difference ( p
    Figure Legend Snippet: Protein solubility of ( A ) raw and ( B ) cooked noodles with 0%, 0.5%, 1.0%, 1.5% and 2.0% transglutaminase in extracting reagents phosphate buffer (PB; 0.1 M; pH 6.8), 8 M urea in PB (PB + Urea), 0.05 M dithiothreitol in PB (PB + DTT) and 0.05 M sodium dodecyl sulphate in PB (PB + SDS); * indicates significant difference ( p

    Techniques Used: Solubility

    2) Product Images from "Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation"

    Article Title: Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation

    Journal: Redox Biology

    doi: 10.1016/j.redox.2020.101432

    Ibrutinib promotes electrical remodeling associated with atrial fibrillation (AF) development. (A–B) Representative recordings of transient Ca 2+ changes (n = 10 cells per group, Student's t-test) and the sarcoplasmic reticulum (SR) Ca 2+ content (n = 5 cells per group, Student's t-test). (C–G) Quantification of the amplitude, decay time, time to peak of calcium release, and the amplitude of caffeine-induced Ca 2+ release between the control and ibrutinib-treated groups (n = 10 cells per group; Student's t-test). (H–K) Representative line-scan confocal images and the quantification of Ca 2+ sparks (CaSF) in control group mice and ibrutinib group mice (n = 10 cells per group; Student's t-test). FDHM, spark duration; FWHD, spark width. (L) Representative images showing the MitoSOX fluorescence intensity in a single atrial myocyte at different times (n = 5 cells per group). Scale bar: 50 μm. (M) Quantification of mitochondrial reactive oxygen species (ROS) production in the control group, ibrutinib group, and DL-Dithiothreitol (DTT) group (n = 5 cells per group; one way ANOVA). Values are presented as mean ± SD. *P
    Figure Legend Snippet: Ibrutinib promotes electrical remodeling associated with atrial fibrillation (AF) development. (A–B) Representative recordings of transient Ca 2+ changes (n = 10 cells per group, Student's t-test) and the sarcoplasmic reticulum (SR) Ca 2+ content (n = 5 cells per group, Student's t-test). (C–G) Quantification of the amplitude, decay time, time to peak of calcium release, and the amplitude of caffeine-induced Ca 2+ release between the control and ibrutinib-treated groups (n = 10 cells per group; Student's t-test). (H–K) Representative line-scan confocal images and the quantification of Ca 2+ sparks (CaSF) in control group mice and ibrutinib group mice (n = 10 cells per group; Student's t-test). FDHM, spark duration; FWHD, spark width. (L) Representative images showing the MitoSOX fluorescence intensity in a single atrial myocyte at different times (n = 5 cells per group). Scale bar: 50 μm. (M) Quantification of mitochondrial reactive oxygen species (ROS) production in the control group, ibrutinib group, and DL-Dithiothreitol (DTT) group (n = 5 cells per group; one way ANOVA). Values are presented as mean ± SD. *P

    Techniques Used: Mouse Assay, Fluorescence

    3) Product Images from "The orphan nuclear receptor SHP regulates ER stress response by inhibiting XBP1s degradation"

    Article Title: The orphan nuclear receptor SHP regulates ER stress response by inhibiting XBP1s degradation

    Journal: Genes & Development

    doi: 10.1101/gad.326868.119

    SHP expression in pancreatic acinar cells is induced by ER stress in an IRE1α–XBP1s-dependent manner. ( A ) Genomic tagging of endogenous Shp gene loci with 3XFlag in the 3XFlag-Shp knock-in (KI) mice ( top panel) and Western blot analysis of SHP expression in different tissues ( bottom panel). (Panc) Pancreas; (WAT) white adipose tissue; (BAT) brown adipose tissue; (WT) wild-type mice for negative control; (βactin) a loading control. ( B ) Quantitative PCR (qPCR) analysis of pancreatic Shp mRNA levels in WT mice treated with dithiothreitol (DTT) at 0.75 mmol per kilogram of body weight for 6 h. ( C , D ) qPCR analysis of Shp mRNA levels in AR42J acinar cells ( C ) or primary acinar cells ( D ) treated with vehicle (Veh), 100 nM thapsigargin (Tg), or 2 mM DTT for 4 h. ( E , F ) qPCR analysis of mRNA levels of Shp ( E ) and Erdj4 ( F ) in AR42J cells treated with vehicle, 30 µM IRE1α inhibitor 4µ8C, and/or 2 mM DTT for 4 h. ( G , H ) qPCR analysis of mRNA levels of Shp ( G ) and Erdj4 ( H ) in Xbp1 knockout AR42J cells (KO-1 and KO-2) or control cells (CON) with a nonspecific targeting guide, treated with vehicle or 2 mM DTT for 4 h. ( I ) Alignment of the human, rat, and mouse Shp promoters highlighting the putative XBP1s-binding site (underlined). ( J , K ) Luciferase assays using the mouse Shp promoter (from −492 to +36) showing that Shp is a direct transcriptional target of XBP1s. ( J ) Sequence of mouse Shp promoter with a mutated (mut) or deleted (del) putative XBP1s-binding site. ( K ) Cotransfection assay in HEK293T cells expressing luciferase reporter plasmids driven by Shp promoters as indicated and an XBP1s-expressing plasmid. ( L ) Chromatin immunoprecipitation (ChIP) and qPCR analysis showing enrichment of XBP1s at the Shp and BiP promoters. 266-6 pancreatic acinar cells were transfected with an XBP1s-Flag-expressing plasmid or empty plasmid (control [CON]). ChIP was performed with normal rabbit IgG (IgG) or anti-Flag antibody. The positions of qPCR amplicons relative to the transcription initiation site of each locus are indicated. Representative data of two experiments are shown. In B – K , data are represented as mean ± SEM. (*) P
    Figure Legend Snippet: SHP expression in pancreatic acinar cells is induced by ER stress in an IRE1α–XBP1s-dependent manner. ( A ) Genomic tagging of endogenous Shp gene loci with 3XFlag in the 3XFlag-Shp knock-in (KI) mice ( top panel) and Western blot analysis of SHP expression in different tissues ( bottom panel). (Panc) Pancreas; (WAT) white adipose tissue; (BAT) brown adipose tissue; (WT) wild-type mice for negative control; (βactin) a loading control. ( B ) Quantitative PCR (qPCR) analysis of pancreatic Shp mRNA levels in WT mice treated with dithiothreitol (DTT) at 0.75 mmol per kilogram of body weight for 6 h. ( C , D ) qPCR analysis of Shp mRNA levels in AR42J acinar cells ( C ) or primary acinar cells ( D ) treated with vehicle (Veh), 100 nM thapsigargin (Tg), or 2 mM DTT for 4 h. ( E , F ) qPCR analysis of mRNA levels of Shp ( E ) and Erdj4 ( F ) in AR42J cells treated with vehicle, 30 µM IRE1α inhibitor 4µ8C, and/or 2 mM DTT for 4 h. ( G , H ) qPCR analysis of mRNA levels of Shp ( G ) and Erdj4 ( H ) in Xbp1 knockout AR42J cells (KO-1 and KO-2) or control cells (CON) with a nonspecific targeting guide, treated with vehicle or 2 mM DTT for 4 h. ( I ) Alignment of the human, rat, and mouse Shp promoters highlighting the putative XBP1s-binding site (underlined). ( J , K ) Luciferase assays using the mouse Shp promoter (from −492 to +36) showing that Shp is a direct transcriptional target of XBP1s. ( J ) Sequence of mouse Shp promoter with a mutated (mut) or deleted (del) putative XBP1s-binding site. ( K ) Cotransfection assay in HEK293T cells expressing luciferase reporter plasmids driven by Shp promoters as indicated and an XBP1s-expressing plasmid. ( L ) Chromatin immunoprecipitation (ChIP) and qPCR analysis showing enrichment of XBP1s at the Shp and BiP promoters. 266-6 pancreatic acinar cells were transfected with an XBP1s-Flag-expressing plasmid or empty plasmid (control [CON]). ChIP was performed with normal rabbit IgG (IgG) or anti-Flag antibody. The positions of qPCR amplicons relative to the transcription initiation site of each locus are indicated. Representative data of two experiments are shown. In B – K , data are represented as mean ± SEM. (*) P

    Techniques Used: Expressing, Knock-In, Mouse Assay, Western Blot, Negative Control, Real-time Polymerase Chain Reaction, Knock-Out, Binding Assay, Luciferase, Sequencing, Cotransfection, Plasmid Preparation, Chromatin Immunoprecipitation, Transfection

    4) Product Images from "Redox Regulation of the Tumor Suppressor PTEN by Hydrogen Peroxide and Tert-Butyl Hydroperoxide"

    Article Title: Redox Regulation of the Tumor Suppressor PTEN by Hydrogen Peroxide and Tert-Butyl Hydroperoxide

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms18050982

    Effects of t -BHP on redox state of recombinant PTEN. Purified recombinant PTEN (oxidized form) was pre-reduced with 1 mM dithiothreitol (DTT) for 2 h in degassed assay buffer prior to being applied to NAP-5 desalting column equilibrated with degassed assay buffer to remove excess DTT. Samples were then incubated with the indicated concentration of t -BHP for 30 min ( A ), or for increasing periods of time with 0.5 mM t -BHP ( B ). After indicated treatment, samples were alkylated with 2 mM NEM. All samples were fractionated by non-reducing SDS-PAGE followed by immunoblot analysis with PTEN antibody. All blot data are representative of at least three separate experiments.
    Figure Legend Snippet: Effects of t -BHP on redox state of recombinant PTEN. Purified recombinant PTEN (oxidized form) was pre-reduced with 1 mM dithiothreitol (DTT) for 2 h in degassed assay buffer prior to being applied to NAP-5 desalting column equilibrated with degassed assay buffer to remove excess DTT. Samples were then incubated with the indicated concentration of t -BHP for 30 min ( A ), or for increasing periods of time with 0.5 mM t -BHP ( B ). After indicated treatment, samples were alkylated with 2 mM NEM. All samples were fractionated by non-reducing SDS-PAGE followed by immunoblot analysis with PTEN antibody. All blot data are representative of at least three separate experiments.

    Techniques Used: Recombinant, Purification, Incubation, Concentration Assay, SDS Page

    Related Articles

    MTT Assay:

    Article Title: Effects of copolymer component on the properties of phosphorylcholine micelles
    Article Snippet: .. 2-Bromoisobutyryl bromide (98%), stannous octoate (Sn(Oct)2 ; 95%), copper (I) bromide (CuBr; 99%), pentaerythritol, 2,2′-disulfanediyldiethanol, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), 2,2′-bipyridine (BPy), DL-dithiothreitol (DTT) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA) and used as received. ..

    In Vivo:

    Article Title: The orphan nuclear receptor SHP regulates ER stress response by inhibiting XBP1s degradation
    Article Snippet: .. To induce XBP1s and measure its decay in vivo, mice were injected intraperitoneally with DTT (Sigma, D9779) at 0.75 mmol per kilogram of body weight for 6 h, Tuni (Tocris, 3516) at 1 mg per kilogram of body weight for 4 h, and/or CHX (Millipore, 508739) at 2 mg per kilogram of body weight for 20 min. .. Acute pancreatitis was induced by the administration of six hourly intraperitoneal injections of cerulein (Tocris, 6264) at 50 mg per kilogram of body weight each time, and tissues were collected 1 h after the last injection.

    In Vitro:

    Article Title: In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages
    Article Snippet: .. Materials and instruments CDDP, N -hydroxysuccinimide (NHS), N -(3-dimethyl-aminopropyl)-N ′-ethylcarbodiimide hydrochloride (EDC·HCl), hydrazine monohydrate, N ,N ′-dicyclohexylcarbodiimide (DCC), N ,N -diisopropylethylamine (DIPEA), 9-mercapto-1-nonanol, 3-mercaptopropionic acid (3-MPA), 2-hydroxyethyl disulfide, DL-dithiothreitol (DTT), esterase from porcine liver, ultrapure nitric acid (65%, for ultratrace analysis), LDH in vitro toxicology assay kit, and McCoy’s 5A medium were purchased from Sigma-Aldrich. .. Nitric acid (65%) and sulfuric acid (98%) were purchased from Merck.

    Mouse Assay:

    Article Title: The orphan nuclear receptor SHP regulates ER stress response by inhibiting XBP1s degradation
    Article Snippet: .. To induce XBP1s and measure its decay in vivo, mice were injected intraperitoneally with DTT (Sigma, D9779) at 0.75 mmol per kilogram of body weight for 6 h, Tuni (Tocris, 3516) at 1 mg per kilogram of body weight for 4 h, and/or CHX (Millipore, 508739) at 2 mg per kilogram of body weight for 20 min. .. Acute pancreatitis was induced by the administration of six hourly intraperitoneal injections of cerulein (Tocris, 6264) at 50 mg per kilogram of body weight each time, and tissues were collected 1 h after the last injection.

    Droplet Countercurrent Chromatography:

    Article Title: In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages
    Article Snippet: .. Materials and instruments CDDP, N -hydroxysuccinimide (NHS), N -(3-dimethyl-aminopropyl)-N ′-ethylcarbodiimide hydrochloride (EDC·HCl), hydrazine monohydrate, N ,N ′-dicyclohexylcarbodiimide (DCC), N ,N -diisopropylethylamine (DIPEA), 9-mercapto-1-nonanol, 3-mercaptopropionic acid (3-MPA), 2-hydroxyethyl disulfide, DL-dithiothreitol (DTT), esterase from porcine liver, ultrapure nitric acid (65%, for ultratrace analysis), LDH in vitro toxicology assay kit, and McCoy’s 5A medium were purchased from Sigma-Aldrich. .. Nitric acid (65%) and sulfuric acid (98%) were purchased from Merck.

    Activated Clotting Time Assay:

    Article Title: A redox mechanism underlying nucleolar stress sensing by nucleophosmin
    Article Snippet: .. GSSG (G2140), H2 O2 (H1009), NAC (A9165), DTT (D0632) and Act.D (A4262) were purchased from Sigma-Aldrich. .. Nutlin-3 (3984) was obtained from Tocris Bioscience.

    Injection:

    Article Title: The orphan nuclear receptor SHP regulates ER stress response by inhibiting XBP1s degradation
    Article Snippet: .. To induce XBP1s and measure its decay in vivo, mice were injected intraperitoneally with DTT (Sigma, D9779) at 0.75 mmol per kilogram of body weight for 6 h, Tuni (Tocris, 3516) at 1 mg per kilogram of body weight for 4 h, and/or CHX (Millipore, 508739) at 2 mg per kilogram of body weight for 20 min. .. Acute pancreatitis was induced by the administration of six hourly intraperitoneal injections of cerulein (Tocris, 6264) at 50 mg per kilogram of body weight each time, and tissues were collected 1 h after the last injection.

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