dl dithiothreitol  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Millipore dl dithiothreitol
    Dl Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dl dithiothreitol/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dl dithiothreitol - by Bioz Stars, 2020-03
    99/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus inhibits NF-κB signaling by means of its deubiquitinating activity.
    Article Snippet: .. At 30 h post transfection, cells were harvested by adding 250 L lysis buffer A (LBA) (65 mM Tris-HCl [pH 6.8], 4% sodium dodecyl sulfate, 3% DL-dithiothreitol, and 40% glycerol) containing 20 mM N-ethylmaleimide (NEM) (Sigma) and 20 mM iodoacetamine (Sigma). .. Cell lysates were then analyzed for expression of ubiquitin-conjugated proteins by Western blot with an anti-HA antibody (1:1,000) (MBL, Japan).

    Amplification:

    Article Title: N6-methyladenine DNA Modification in Glioblastoma
    Article Snippet: The samples were permeabilized in cold permabilization buffer (0.2% IGEPAL-CA630 (I8896, Sigma), 1 mM DTT (D9779, Sigma), Protease inhibitor (05056489001, Roche), 5% BSA (A7906, Sigma) in PBS (10010-23, Thermo Fisher Scientific)) for 10 minutes on the rotator in the cold room and centrifuged for 5 min at 500 xg at 4°C. .. The libraries were amplified using NEBNext High-Fidelity 2X PCR Master Mix (M0541, NEB) with primer extension at 72°C for 5 minutes, denaturation at 98°C for 30s, followed by 8 cycles of denaturation at 98°C for 10 s, annealing at 63°C for 30 seconds and extension at 72°C for 60 seconds.

    Sample Prep:

    Article Title: Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry.
    Article Snippet: Paragraph title: Sample preparation of cultured viruses ... A 200 l aliquot was mixed with 25 l of a 0.5% stock solution of RapiGest (Waters Corporation, Milford, CT, USA) and was incubated for 7 min at 95 • C. Next, DL-dithiothreitol (DTT, Sigma-Aldrich) and acetonitrile (LC-MS Chromasolv, Sigma-Aldrich) were added to 5 mM and to 25% final concentration, respectively.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry.
    Article Snippet: .. A 200 l aliquot was mixed with 25 l of a 0.5% stock solution of RapiGest (Waters Corporation, Milford, CT, USA) and was incubated for 7 min at 95 • C. Next, DL-dithiothreitol (DTT, Sigma-Aldrich) and acetonitrile (LC-MS Chromasolv, Sigma-Aldrich) were added to 5 mM and to 25% final concentration, respectively. ..

    Mutagenesis:

    Article Title: The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus inhibits NF-κB signaling by means of its deubiquitinating activity.
    Article Snippet: Assay of DUB activity HEK293 cells cultured in 60-mm dishes were cotransfected with 1.2 g of HA-Ub, HA-K48-Ub, or HA-K63-Ub plus appropriate amounts of vector encoding wild-type PRRSV Nsp11 or the indicated mutant using Lipofectamine 2000 (Invitrogen). .. At 30 h post transfection, cells were harvested by adding 250 L lysis buffer A (LBA) (65 mM Tris-HCl [pH 6.8], 4% sodium dodecyl sulfate, 3% DL-dithiothreitol, and 40% glycerol) containing 20 mM N-ethylmaleimide (NEM) (Sigma) and 20 mM iodoacetamine (Sigma).

    Protease Inhibitor:

    Article Title: N6-methyladenine DNA Modification in Glioblastoma
    Article Snippet: .. The samples were permeabilized in cold permabilization buffer (0.2% IGEPAL-CA630 (I8896, Sigma), 1 mM DTT (D9779, Sigma), Protease inhibitor (05056489001, Roche), 5% BSA (A7906, Sigma) in PBS (10010-23, Thermo Fisher Scientific)) for 10 minutes on the rotator in the cold room and centrifuged for 5 min at 500 xg at 4°C. .. The pellet was resuspended in cold tagmentation buffer (33 mM Tris-acetate (pH = 7.8) (BP-152, Thermo Fisher Scientific), 66 mM K-acetate (P5708, Sigma), 11 mM Mg-acetate (M2545, Sigma), 16 % DMF (DX1730, EMD Millipore) in Molecular biology water (46000-CM, Corning)) and incubated with Tagmentation enzyme (FC-121-1030; Illumina) at 37 °C for 30 min with shaking 500 rpm.

    Preserving:

    Article Title: Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry.
    Article Snippet: CyMol is a collection, transport, and preservation medium for cells (cytology analysis), viruses, and nucleic acids (PCR-based assays) (Luinstra et al., 2011a) . .. A 200 l aliquot was mixed with 25 l of a 0.5% stock solution of RapiGest (Waters Corporation, Milford, CT, USA) and was incubated for 7 min at 95 • C. Next, DL-dithiothreitol (DTT, Sigma-Aldrich) and acetonitrile (LC-MS Chromasolv, Sigma-Aldrich) were added to 5 mM and to 25% final concentration, respectively.

    Generated:

    Article Title: A comprehensive pipeline for translational top-down proteomics from a single blood draw
    Article Snippet: Sodium chloride (Sigma-Aldrich, cat. no. S9888) DL-Dithiothreitol (DTT; Sigma-Aldrich, cat. no. D0632) !CAUTION Reagent is harmful if swallowed and may cause skin, eye, and respiratory irritation. .. Magnesium chloride (Sigma-Aldrich, cat. no. M8266) !CAUTION Avoid breathing dust or vapors generated by this product.

    Cell Culture:

    Article Title: A comprehensive pipeline for translational top-down proteomics from a single blood draw
    Article Snippet: FBS (USDA approved, sterile-filtered, suitable for cell culture; Sigma-Aldrich, cat. no. F0926) HyClone Dulbecco’s PBS (DPBS, without calcium, magnesium; GE Healthcare Life Science, cat. no. SH30028.02) DMSO for molecular biology (Sigma-Aldrich, cat. no. D8418) N-Lauroylsarcosine sodium salt (Sigma-Aldrich, cat. no. L9150–50G) !CAUTION Lauroylsarcosine sodium salt causes skin irritation and serious eye damage, and may be fatal if inhaled. .. Sodium chloride (Sigma-Aldrich, cat. no. S9888) DL-Dithiothreitol (DTT; Sigma-Aldrich, cat. no. D0632) !CAUTION Reagent is harmful if swallowed and may cause skin, eye, and respiratory irritation.

    Article Title: Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry.
    Article Snippet: Paragraph title: Sample preparation of cultured viruses ... A 200 l aliquot was mixed with 25 l of a 0.5% stock solution of RapiGest (Waters Corporation, Milford, CT, USA) and was incubated for 7 min at 95 • C. Next, DL-dithiothreitol (DTT, Sigma-Aldrich) and acetonitrile (LC-MS Chromasolv, Sigma-Aldrich) were added to 5 mM and to 25% final concentration, respectively.

    Article Title: The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus inhibits NF-κB signaling by means of its deubiquitinating activity.
    Article Snippet: Assay of DUB activity HEK293 cells cultured in 60-mm dishes were cotransfected with 1.2 g of HA-Ub, HA-K48-Ub, or HA-K63-Ub plus appropriate amounts of vector encoding wild-type PRRSV Nsp11 or the indicated mutant using Lipofectamine 2000 (Invitrogen). .. At 30 h post transfection, cells were harvested by adding 250 L lysis buffer A (LBA) (65 mM Tris-HCl [pH 6.8], 4% sodium dodecyl sulfate, 3% DL-dithiothreitol, and 40% glycerol) containing 20 mM N-ethylmaleimide (NEM) (Sigma) and 20 mM iodoacetamine (Sigma).

    Purification:

    Article Title: N6-methyladenine DNA Modification in Glioblastoma
    Article Snippet: The samples were permeabilized in cold permabilization buffer (0.2% IGEPAL-CA630 (I8896, Sigma), 1 mM DTT (D9779, Sigma), Protease inhibitor (05056489001, Roche), 5% BSA (A7906, Sigma) in PBS (10010-23, Thermo Fisher Scientific)) for 10 minutes on the rotator in the cold room and centrifuged for 5 min at 500 xg at 4°C. .. The tagementated DNA was purified using MinElute PCR purification kit (28004, Qiagen).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Ultrasensitive detection of circulating exosomes with a 3D-nanopatterned microfluidic chip
    Article Snippet: The ELISA kits for EpCAM, CD24 and FRα were ordered from R & D Systems. .. SβG and FDG were dissolved in PBS working solution (PBSW) at pH 7.4 which contain 0.5 mM DL-dithiothreitol (Sigma-Aldrich), 2 mM MgCl2 (Fluka Analytical), and 5% bovine serum albumin (BSA, Sigma-Aldrich).

    Concentration Assay:

    Article Title: Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry.
    Article Snippet: .. A 200 l aliquot was mixed with 25 l of a 0.5% stock solution of RapiGest (Waters Corporation, Milford, CT, USA) and was incubated for 7 min at 95 • C. Next, DL-dithiothreitol (DTT, Sigma-Aldrich) and acetonitrile (LC-MS Chromasolv, Sigma-Aldrich) were added to 5 mM and to 25% final concentration, respectively. ..

    Incubation:

    Article Title: Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry.
    Article Snippet: .. A 200 l aliquot was mixed with 25 l of a 0.5% stock solution of RapiGest (Waters Corporation, Milford, CT, USA) and was incubated for 7 min at 95 • C. Next, DL-dithiothreitol (DTT, Sigma-Aldrich) and acetonitrile (LC-MS Chromasolv, Sigma-Aldrich) were added to 5 mM and to 25% final concentration, respectively. ..

    Article Title: N6-methyladenine DNA Modification in Glioblastoma
    Article Snippet: The samples were permeabilized in cold permabilization buffer (0.2% IGEPAL-CA630 (I8896, Sigma), 1 mM DTT (D9779, Sigma), Protease inhibitor (05056489001, Roche), 5% BSA (A7906, Sigma) in PBS (10010-23, Thermo Fisher Scientific)) for 10 minutes on the rotator in the cold room and centrifuged for 5 min at 500 xg at 4°C. .. The pellet was resuspended in cold tagmentation buffer (33 mM Tris-acetate (pH = 7.8) (BP-152, Thermo Fisher Scientific), 66 mM K-acetate (P5708, Sigma), 11 mM Mg-acetate (M2545, Sigma), 16 % DMF (DX1730, EMD Millipore) in Molecular biology water (46000-CM, Corning)) and incubated with Tagmentation enzyme (FC-121-1030; Illumina) at 37 °C for 30 min with shaking 500 rpm.

    Activity Assay:

    Article Title: The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus inhibits NF-κB signaling by means of its deubiquitinating activity.
    Article Snippet: Paragraph title: Assay of DUB activity ... At 30 h post transfection, cells were harvested by adding 250 L lysis buffer A (LBA) (65 mM Tris-HCl [pH 6.8], 4% sodium dodecyl sulfate, 3% DL-dithiothreitol, and 40% glycerol) containing 20 mM N-ethylmaleimide (NEM) (Sigma) and 20 mM iodoacetamine (Sigma).

    Expressing:

    Article Title: The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus inhibits NF-κB signaling by means of its deubiquitinating activity.
    Article Snippet: At 30 h post transfection, cells were harvested by adding 250 L lysis buffer A (LBA) (65 mM Tris-HCl [pH 6.8], 4% sodium dodecyl sulfate, 3% DL-dithiothreitol, and 40% glycerol) containing 20 mM N-ethylmaleimide (NEM) (Sigma) and 20 mM iodoacetamine (Sigma). .. Cell lysates were then analyzed for expression of ubiquitin-conjugated proteins by Western blot with an anti-HA antibody (1:1,000) (MBL, Japan).

    Staining:

    Article Title: Ultrasensitive detection of circulating exosomes with a 3D-nanopatterned microfluidic chip
    Article Snippet: Streptavidin conjugated β-Galactosidase (SβG), fluorescein-di-β-D-galactopyranoside (FDG), 3,3’-dioctadecyloxacarbocyanine perchlorate (DiO) lipophilic dye, Vybrant™ CM-DiO cell staining solution, and fluorescent nanoparticles (5% solid, Ex/Em 540/560 nm, actual size 46 nm) were purchased from Life Technologies. .. SβG and FDG were dissolved in PBS working solution (PBSW) at pH 7.4 which contain 0.5 mM DL-dithiothreitol (Sigma-Aldrich), 2 mM MgCl2 (Fluka Analytical), and 5% bovine serum albumin (BSA, Sigma-Aldrich).

    Polymerase Chain Reaction:

    Article Title: Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry.
    Article Snippet: CyMol is a collection, transport, and preservation medium for cells (cytology analysis), viruses, and nucleic acids (PCR-based assays) (Luinstra et al., 2011a) . .. A 200 l aliquot was mixed with 25 l of a 0.5% stock solution of RapiGest (Waters Corporation, Milford, CT, USA) and was incubated for 7 min at 95 • C. Next, DL-dithiothreitol (DTT, Sigma-Aldrich) and acetonitrile (LC-MS Chromasolv, Sigma-Aldrich) were added to 5 mM and to 25% final concentration, respectively.

    Article Title: N6-methyladenine DNA Modification in Glioblastoma
    Article Snippet: The samples were permeabilized in cold permabilization buffer (0.2% IGEPAL-CA630 (I8896, Sigma), 1 mM DTT (D9779, Sigma), Protease inhibitor (05056489001, Roche), 5% BSA (A7906, Sigma) in PBS (10010-23, Thermo Fisher Scientific)) for 10 minutes on the rotator in the cold room and centrifuged for 5 min at 500 xg at 4°C. .. The tagementated DNA was purified using MinElute PCR purification kit (28004, Qiagen).

    Western Blot:

    Article Title: The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus inhibits NF-κB signaling by means of its deubiquitinating activity.
    Article Snippet: At 30 h post transfection, cells were harvested by adding 250 L lysis buffer A (LBA) (65 mM Tris-HCl [pH 6.8], 4% sodium dodecyl sulfate, 3% DL-dithiothreitol, and 40% glycerol) containing 20 mM N-ethylmaleimide (NEM) (Sigma) and 20 mM iodoacetamine (Sigma). .. Cell lysates were then analyzed for expression of ubiquitin-conjugated proteins by Western blot with an anti-HA antibody (1:1,000) (MBL, Japan).

    Lysis:

    Article Title: A comprehensive pipeline for translational top-down proteomics from a single blood draw
    Article Snippet: Paragraph title: PBMC collection and cell lysis (Reagent setup) ... Sodium chloride (Sigma-Aldrich, cat. no. S9888) DL-Dithiothreitol (DTT; Sigma-Aldrich, cat. no. D0632) !CAUTION Reagent is harmful if swallowed and may cause skin, eye, and respiratory irritation.

    Article Title: The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus inhibits NF-κB signaling by means of its deubiquitinating activity.
    Article Snippet: .. At 30 h post transfection, cells were harvested by adding 250 L lysis buffer A (LBA) (65 mM Tris-HCl [pH 6.8], 4% sodium dodecyl sulfate, 3% DL-dithiothreitol, and 40% glycerol) containing 20 mM N-ethylmaleimide (NEM) (Sigma) and 20 mM iodoacetamine (Sigma). .. Cell lysates were then analyzed for expression of ubiquitin-conjugated proteins by Western blot with an anti-HA antibody (1:1,000) (MBL, Japan).

    Plasmid Preparation:

    Article Title: The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus inhibits NF-κB signaling by means of its deubiquitinating activity.
    Article Snippet: Where applicable, the empty vector was supplemented to keep the total amount of DNA transfected constant. .. At 30 h post transfection, cells were harvested by adding 250 L lysis buffer A (LBA) (65 mM Tris-HCl [pH 6.8], 4% sodium dodecyl sulfate, 3% DL-dithiothreitol, and 40% glycerol) containing 20 mM N-ethylmaleimide (NEM) (Sigma) and 20 mM iodoacetamine (Sigma).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore dl dtt
    Dl Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dl dtt/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    dl dtt - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Millipore buffer
    Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer/product/Millipore
    Average 99 stars, based on 256 article reviews
    Price from $9.99 to $1999.99
    buffer - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results