disuccinimidyl glutarate  (Millipore)


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    Structured Review

    Millipore disuccinimidyl glutarate
    Disuccinimidyl Glutarate, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/disuccinimidyl glutarate/product/Millipore
    Average 94 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    disuccinimidyl glutarate - by Bioz Stars, 2020-05
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
    Article Snippet: .. Briefly, cells (1–2 × 108 ) were fixed in 2 mM Di(N-succinimidyl) glutarate (DSG) (80424, Sigma) in PBS at room temperature (RT) for 45 min. After washing in PBS, cells were fixed again in 1% formaldehyde in TS base media at RT for 12 min. Fixation was stopped by adding glycine to a final concentration of 0.125 M. Cells were washed twice in PBS and resuspended in wash buffer 1 (10 mM Hepes pH 7.5, 10 mM EDTA, 0.5 mM EGTA and 0.75% Triton X-100) and incubated at 4 °C for 10 min. After pelleting, cells were resuspended in wash buffer 2 (10 mM Hepes pH 7.5, 200 mM NaCl, 1 mM EDTA and 0.5 mM EGTA) and incubated at 4 °C for 10 min. After pelleting, cells were lysed in the lysis/sonication buffer (150 mM NaCl, 25 mM Tris pH 7.5, 5 mM EDTA, 0.1% Triton, 1% SDS and 0.5% sodium deoxycholate) with complete protease inhibitors (Roche) on ice for 30 min. Chromatin was sonicated 30 s on/30 off for 25–30 cycles using the BioRuptor (Diagenode) to the average 300-bp fragments. ..

    Article Title: Signal Integration of IFN-I and IFN-II With TLR4 Involves Sequential Recruitment of STAT1-Complexes and NFκB to Enhance Pro-inflammatory Transcription
    Article Snippet: .. Briefly, primary WT and STAT1−/− VSMC treated as described above were double-cross-linked with 0.5 M DSG (Sigma-Aldrich, 80424) for 45 min followed by 1% formaldehyde (TFS, 28906) for 10 min. Glycine (Sigma-Aldrich, G7126) was added for 10 min in 125 mM final concentration to stop cross-linking process. .. After fixation, nuclei were isolated by addition of ChIP Lysis Buffer (1% Triton X-100 (Bio-Shop, TRX777), 0.1% SDS (Bio-Shop, SDS001), 150 mM NaCl (Sigma-Aldrich, S9888), 1 mM EDTA (TFS, 15575-038), and 20 mM Tris, pH 8.0 (TFS, 15568-025).

    Incubation:

    Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
    Article Snippet: .. Briefly, cells (1–2 × 108 ) were fixed in 2 mM Di(N-succinimidyl) glutarate (DSG) (80424, Sigma) in PBS at room temperature (RT) for 45 min. After washing in PBS, cells were fixed again in 1% formaldehyde in TS base media at RT for 12 min. Fixation was stopped by adding glycine to a final concentration of 0.125 M. Cells were washed twice in PBS and resuspended in wash buffer 1 (10 mM Hepes pH 7.5, 10 mM EDTA, 0.5 mM EGTA and 0.75% Triton X-100) and incubated at 4 °C for 10 min. After pelleting, cells were resuspended in wash buffer 2 (10 mM Hepes pH 7.5, 200 mM NaCl, 1 mM EDTA and 0.5 mM EGTA) and incubated at 4 °C for 10 min. After pelleting, cells were lysed in the lysis/sonication buffer (150 mM NaCl, 25 mM Tris pH 7.5, 5 mM EDTA, 0.1% Triton, 1% SDS and 0.5% sodium deoxycholate) with complete protease inhibitors (Roche) on ice for 30 min. Chromatin was sonicated 30 s on/30 off for 25–30 cycles using the BioRuptor (Diagenode) to the average 300-bp fragments. ..

    Chromatin Immunoprecipitation:

    Article Title: Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells
    Article Snippet: .. For TET1 ChIP, cells were fixed with an initial cross-linking step of 45 min with mM Di(N-succinimidyl)glutarate (Sigma-Aldrich, #80424) in PBS at room temperature followed by formaldehyde fixation, as previously described [ ]. .. Each pellet containing 1 × 107 cells was lysed, resuspended in 1 mL of sonication buffer on ice (10 mM Tris at pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.1% NaDOC, 0.25% NLS, and protease inhibitors), transferred to TC 12 × 12 tubes (Covaris) and sonicated (Covaris settings 20 min, 5% duty cycle, 140 W, 200 cycles).

    Article Title: EZH2 regulates neuroepithelium structure and neuroblast proliferation by repressing p21
    Article Snippet: .. Basically, cells were fixed with 1% formaldehyde for 10 min at room temperature; in addition, for EZH2 ChIP a previous fixation step with 2 mM DSG (Sigma 80424) was performed for 40 min at room temperature. ..

    Article Title: SMADs and YAP Compete to Control Elongation of β-Catenin:LEF-1-Recruited RNAPII during hESC Differentiation
    Article Snippet: .. For the ChIP experiments, 3 × 106 hESCs were double-crosslinked with 0.2 mM di (N-succinimidyl) glutarate (DSG, Sigma, 80424) followed by 1% formaldehyde. .. The cell lysate was precleared and incubated with antibody overnight at 4°C.

    Lysis:

    Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
    Article Snippet: .. Briefly, cells (1–2 × 108 ) were fixed in 2 mM Di(N-succinimidyl) glutarate (DSG) (80424, Sigma) in PBS at room temperature (RT) for 45 min. After washing in PBS, cells were fixed again in 1% formaldehyde in TS base media at RT for 12 min. Fixation was stopped by adding glycine to a final concentration of 0.125 M. Cells were washed twice in PBS and resuspended in wash buffer 1 (10 mM Hepes pH 7.5, 10 mM EDTA, 0.5 mM EGTA and 0.75% Triton X-100) and incubated at 4 °C for 10 min. After pelleting, cells were resuspended in wash buffer 2 (10 mM Hepes pH 7.5, 200 mM NaCl, 1 mM EDTA and 0.5 mM EGTA) and incubated at 4 °C for 10 min. After pelleting, cells were lysed in the lysis/sonication buffer (150 mM NaCl, 25 mM Tris pH 7.5, 5 mM EDTA, 0.1% Triton, 1% SDS and 0.5% sodium deoxycholate) with complete protease inhibitors (Roche) on ice for 30 min. Chromatin was sonicated 30 s on/30 off for 25–30 cycles using the BioRuptor (Diagenode) to the average 300-bp fragments. ..

    Sonication:

    Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
    Article Snippet: .. Briefly, cells (1–2 × 108 ) were fixed in 2 mM Di(N-succinimidyl) glutarate (DSG) (80424, Sigma) in PBS at room temperature (RT) for 45 min. After washing in PBS, cells were fixed again in 1% formaldehyde in TS base media at RT for 12 min. Fixation was stopped by adding glycine to a final concentration of 0.125 M. Cells were washed twice in PBS and resuspended in wash buffer 1 (10 mM Hepes pH 7.5, 10 mM EDTA, 0.5 mM EGTA and 0.75% Triton X-100) and incubated at 4 °C for 10 min. After pelleting, cells were resuspended in wash buffer 2 (10 mM Hepes pH 7.5, 200 mM NaCl, 1 mM EDTA and 0.5 mM EGTA) and incubated at 4 °C for 10 min. After pelleting, cells were lysed in the lysis/sonication buffer (150 mM NaCl, 25 mM Tris pH 7.5, 5 mM EDTA, 0.1% Triton, 1% SDS and 0.5% sodium deoxycholate) with complete protease inhibitors (Roche) on ice for 30 min. Chromatin was sonicated 30 s on/30 off for 25–30 cycles using the BioRuptor (Diagenode) to the average 300-bp fragments. ..

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  • 90
    Millipore disuccinimidyl glutarate
    Disuccinimidyl Glutarate, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/disuccinimidyl glutarate/product/Millipore
    Average 90 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    disuccinimidyl glutarate - by Bioz Stars, 2020-05
    90/100 stars
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