disuccinimidyl glutarate  (Millipore)


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    Structured Review

    Millipore disuccinimidyl glutarate
    Disuccinimidyl Glutarate, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/disuccinimidyl glutarate/product/Millipore
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    disuccinimidyl glutarate - by Bioz Stars, 2019-10
    94/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription
    Article Snippet: For JMJD1a, ChIP cells were crosslinked with disuccinimidyl glutarate (Sigma) for 45 min followed by 10 min incubation in 1% PFAH. .. Crosslinking reaction was stopped with 0.125 M Glycine by 5-min incubation.

    Article Title: Signal Transducer and Activator of Transcription 1 Plays a Pivotal Role in RET/PTC3 Oncogene-induced Expression of Indoleamine 2,3-Dioxygenase 1
    Article Snippet: In the first instance, disuccinimidyl glutarate (Sigma) was added to a final concentration of 2 m m and cells were incubated 45 min at room temperature for cross-linking. .. After harvesting, cells were lysed in 700 μl of ice-cold cell lysis buffer (5 m m HEPES, pH 8.0, 85 m m KCl, 0.5% Nonidet P-40, 1 m m PMSF, 1× protease inhibitor mixture (Roche)) for 10 min on ice.

    Article Title: Brg1 Enables Rapid Growth of the Early Embryo by Suppressing Genes That Regulate Apoptosis and Cell Growth Arrest
    Article Snippet: For the CHD4 ChIP, formaldehyde fixation was followed by a 45-min incubation in 2 mM disuccinimidyl glutarate (DSG; catalog number 80424-50MG-F; Sigma). .. The cells were lysed in cold lysis buffer 1 (50 mM HEPES-KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1× protease inhibitors) and gently rocked at 4°C for 10 min in 14-ml conical tubes.

    Amplification:

    Article Title: Chromatin regulation by Brg1 underlies heart muscle development and disease
    Article Snippet: Adult hearts were minced before fixing with 1% PFA (for Brg1 or PARP1 ChIP) or with 1% PFA and 2 mM disuccinimidyl glutarate (Sigma) for HDAC ChIP. .. Chromatin was sonicated to generate average fragment sizes of 100-300 bp, and immunoprecipitated using anti-Brg1 J1 antibody , anti-PARP1 antibody (N-20, sc-1561, Santa Cruz Biotechnology, Santa Cruz, CA), anti-HDAC2 (H54; Santa Cruz Biotechnology, Santa Cruz, CA), HDAC9 (ab59718; Abcam, Cambridge, MA), or anti-HRP (AffiniPure rabbit anti-horse radish peroxidase, 323-005-021, Jackson Immunoresearch, West Grove, PA).

    Construct:

    Article Title: Regulator of G Protein Signaling 7 (RGS7) Can Exist in a Homo-oligomeric Form That Is Regulated by Gαo and R7-binding Protein
    Article Snippet: HEK293T cells were transfected with RGS7 constructs together with Gβ5 and other RGS7-interacting proteins depending on the purpose of the experiment. .. Alternatively, cells were treated with 2 μ m disuccinimidyl glutarate (DSG, Sigma) in PBS for 30 min.

    Real-time Polymerase Chain Reaction:

    Article Title: Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity
    Article Snippet: The cells were incubated with 2 mM disuccinimidyl glutarate (Sigma-Aldrich) for 25 min at room temperature and then cross-linked with 1% formaldehyde in medium for 10 min. .. The sheared chromatin was in the range of 300–800 bp and was immunoprecipitated with 3 µg anti-Tle3 antibody (Cat. No. 11372-1-AP, rabbit polyclonal antibody, Proteintech Group) or control rabbit IgG, followed by steps of washing as previously described ( ).

    Article Title: Cell of origin in AML: Susceptibility to MN1-induced transformation is regulated by the MEIS1/AbdB-like HOX protein complex
    Article Snippet: The gene set enrichment analysis software ( ) ( ) was used to compare enrichment of curated gene sets (dataset C2) and Gene Ontology gene sets (dataset C5, available from the Molecular Signature database v2.5 ( ). .. Chromatin immunoprecipitation assays were performed using sequential cross-linking with disuccinimidyl glutarate (Sigma) and formaldehyde followed by immunoprecipitation (see ) using an anti-HA antibody (Abcam ab9110). qPCR was performed to verify enrichment, and Illumina DNA library construction for next generation sequencing was performed as described previously ( ). .. Cluster generation and 50bp sequencing on the Illumina Genome Analyzer IIx (Illumina, San Diego, CA) was performed following the manufacturer’s instructions.

    Incubation:

    Article Title: UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs
    Article Snippet: For SMARCA4, CHD4 and UTX ChIP–qPCR, 416B cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 10 min at RT. .. For SMARCA4, CHD4 and UTX ChIP–qPCR, 416B cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 10 min at RT.

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: CD45.2+ KLRG1hi IL-7Rαlo CD8+ TEFF cells were sorted from CD45.1+ recipient mice on 8 dpi in adoptive transfer and LCMV-Arm infection experiments. .. The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium. .. The fixed cells were processed with a truChIP Chromatin Shearing Reagent Kit (Covaris) and sonicated for 5 min on Covaris S2 ultrasonicator.

    Article Title: Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription
    Article Snippet: Protein–chromatin complexes were crosslinked with 1% paraformaldehyde (PFAH) for 10 min at RT (in case of H3K9me2). .. For JMJD1a, ChIP cells were crosslinked with disuccinimidyl glutarate (Sigma) for 45 min followed by 10 min incubation in 1% PFAH. .. Disuccinimidyl glutarate was used for JMJD1a ChIP since crosslinkers with longer linker arms have been used for JMJD1a ChIP .

    Article Title: Signal Transducer and Activator of Transcription 1 Plays a Pivotal Role in RET/PTC3 Oncogene-induced Expression of Indoleamine 2,3-Dioxygenase 1
    Article Snippet: The cells were washed twice with PBS at room temperature and resuspended in PBS containing 1 m m MgCl2 . .. In the first instance, disuccinimidyl glutarate (Sigma) was added to a final concentration of 2 m m and cells were incubated 45 min at room temperature for cross-linking. .. In the second instance, formaldehyde was added to a final concentration of 1% (v/v) and cells were incubated for 15 min at room temperature for dual cross-linking.

    Article Title: Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity
    Article Snippet: Total thymocytes from VavCre+ Tle3 −/− mice were used as negative control. .. The cells were incubated with 2 mM disuccinimidyl glutarate (Sigma-Aldrich) for 25 min at room temperature and then cross-linked with 1% formaldehyde in medium for 10 min. .. The fixed cells were processed using truChIP Chromatin Shearing Reagent kit (Covaris) and sonicated for 6 min on Covaris S2 ultrasonicator.

    Article Title: Brg1 Enables Rapid Growth of the Early Embryo by Suppressing Genes That Regulate Apoptosis and Cell Growth Arrest
    Article Snippet: Cells were cross-linked by adding 13.5 μl of 36.6% formaldehyde (catalog number 8775; Sigma) per 500 μl of sample for 15 min at room temperature. .. For the CHD4 ChIP, formaldehyde fixation was followed by a 45-min incubation in 2 mM disuccinimidyl glutarate (DSG; catalog number 80424-50MG-F; Sigma). .. The cells were lysed in cold lysis buffer 1 (50 mM HEPES-KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1× protease inhibitors) and gently rocked at 4°C for 10 min in 14-ml conical tubes.

    Article Title: Regulator of G Protein Signaling 7 (RGS7) Can Exist in a Homo-oligomeric Form That Is Regulated by Gαo and R7-binding Protein
    Article Snippet: Two days later, cells were washed two times with phosphate-buffered saline (PBS) and incubated with 1% paraformaldehyde (PFA) in PBS for 30 min. .. Alternatively, cells were treated with 2 μ m disuccinimidyl glutarate (DSG, Sigma) in PBS for 30 min.

    Expressing:

    Article Title: Signal Transducer and Activator of Transcription 1 Plays a Pivotal Role in RET/PTC3 Oncogene-induced Expression of Indoleamine 2,3-Dioxygenase 1
    Article Snippet: Briefly, PTC3.5 cells were treated for 24 h with Dox to induce expression of the RET/PTC3 oncogene. .. In the first instance, disuccinimidyl glutarate (Sigma) was added to a final concentration of 2 m m and cells were incubated 45 min at room temperature for cross-linking.

    Modification:

    Article Title: UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs
    Article Snippet: For SMARCA4, CHD4 and UTX ChIP–qPCR, 416B cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 10 min at RT. .. Cells were resuspended in ChIP Lysis Buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8, and protease inhibitors) and sonicated in a Bioruptor Pico instrument (Diagenode) for ten cycles.

    Transfection:

    Article Title: Regulator of G Protein Signaling 7 (RGS7) Can Exist in a Homo-oligomeric Form That Is Regulated by Gαo and R7-binding Protein
    Article Snippet: HEK293T cells were transfected with RGS7 constructs together with Gβ5 and other RGS7-interacting proteins depending on the purpose of the experiment. .. Alternatively, cells were treated with 2 μ m disuccinimidyl glutarate (DSG, Sigma) in PBS for 30 min.

    Concentration Assay:

    Article Title: Signal Transducer and Activator of Transcription 1 Plays a Pivotal Role in RET/PTC3 Oncogene-induced Expression of Indoleamine 2,3-Dioxygenase 1
    Article Snippet: The cells were washed twice with PBS at room temperature and resuspended in PBS containing 1 m m MgCl2 . .. In the first instance, disuccinimidyl glutarate (Sigma) was added to a final concentration of 2 m m and cells were incubated 45 min at room temperature for cross-linking. .. In the second instance, formaldehyde was added to a final concentration of 1% (v/v) and cells were incubated for 15 min at room temperature for dual cross-linking.

    Article Title: Regulator of G Protein Signaling 7 (RGS7) Can Exist in a Homo-oligomeric Form That Is Regulated by Gαo and R7-binding Protein
    Article Snippet: Alternatively, cells were treated with 2 μ m disuccinimidyl glutarate (DSG, Sigma) in PBS for 30 min. .. Alternatively, cells were treated with 2 μ m disuccinimidyl glutarate (DSG, Sigma) in PBS for 30 min.

    Article Title: The WD40-Repeat Proteins NFC101 and NFC102 Regulate Different Aspects of Maize Development through Chromatin Modification
    Article Snippet: This procedure relies on a first step to cross-link the protein of interest to protein complexes and a second step, with formaldehyde treatment, to cross-link the complexes to DNA. .. Disuccinimidyl glutarate (Sigma-Aldrich) was freshly prepared at 0.5 M in DMSO immediately prior to use and was added to each 50-mL Falcon tube, containing samples in MC buffer, to 2 mM final concentration. .. Fixation was performed for 30 min with vacuum infiltration at room temperature, followed by three sequential washes with 30 mL of MC buffer at room temperature and with gentle rotation.

    Protease Inhibitor:

    Article Title: Signal Transducer and Activator of Transcription 1 Plays a Pivotal Role in RET/PTC3 Oncogene-induced Expression of Indoleamine 2,3-Dioxygenase 1
    Article Snippet: In the first instance, disuccinimidyl glutarate (Sigma) was added to a final concentration of 2 m m and cells were incubated 45 min at room temperature for cross-linking. .. Finally, glycine was added to a final concentration of 125 m m and cells incubated for 5 min at room temperature to quench formaldehyde cross-linking.

    Article Title: Brg1 Enables Rapid Growth of the Early Embryo by Suppressing Genes That Regulate Apoptosis and Cell Growth Arrest
    Article Snippet: For the CHD4 ChIP, formaldehyde fixation was followed by a 45-min incubation in 2 mM disuccinimidyl glutarate (DSG; catalog number 80424-50MG-F; Sigma). .. The cells were lysed in cold lysis buffer 1 (50 mM HEPES-KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1× protease inhibitors) and gently rocked at 4°C for 10 min in 14-ml conical tubes.

    Dissection:

    Article Title: The WD40-Repeat Proteins NFC101 and NFC102 Regulate Different Aspects of Maize Development through Chromatin Modification
    Article Snippet: and leaf blade samples used for chromatin extraction were obtained by manual dissection of V3/V4 seedlings, cut with a razor blade and immediately stored in MC buffer (10 mM NaPi, pH 7, 50 mM NaCl, and 0.1 M Suc) at 4°C for not more than 5 h before cross-linking. .. Disuccinimidyl glutarate (Sigma-Aldrich) was freshly prepared at 0.5 M in DMSO immediately prior to use and was added to each 50-mL Falcon tube, containing samples in MC buffer, to 2 mM final concentration.

    Infection:

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: CD45.2+ KLRG1hi IL-7Rαlo CD8+ TEFF cells were sorted from CD45.1+ recipient mice on 8 dpi in adoptive transfer and LCMV-Arm infection experiments. .. The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium.

    Adoptive Transfer Assay:

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: CD45.2+ KLRG1hi IL-7Rαlo CD8+ TEFF cells were sorted from CD45.1+ recipient mice on 8 dpi in adoptive transfer and LCMV-Arm infection experiments. .. The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium.

    Polymerase Chain Reaction:

    Article Title: Chromatin regulation by Brg1 underlies heart muscle development and disease
    Article Snippet: Adult hearts were minced before fixing with 1% PFA (for Brg1 or PARP1 ChIP) or with 1% PFA and 2 mM disuccinimidyl glutarate (Sigma) for HDAC ChIP. .. Adult hearts were minced before fixing with 1% PFA (for Brg1 or PARP1 ChIP) or with 1% PFA and 2 mM disuccinimidyl glutarate (Sigma) for HDAC ChIP.

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium. .. The sheared chromatin from 3–5 × 106 CD8+ T cells was immunoprecipitated with 5 μg of purified anti-CBFβ antibody and was washed as described .

    Sonication:

    Article Title: Chromatin regulation by Brg1 underlies heart muscle development and disease
    Article Snippet: Adult hearts were minced before fixing with 1% PFA (for Brg1 or PARP1 ChIP) or with 1% PFA and 2 mM disuccinimidyl glutarate (Sigma) for HDAC ChIP. .. Nuclei were isolated by disruption using a B dounce, and washed with SDS lysis buffer (1% SDS, 10mM EDTA and 50mM Tris, pH 8.1).

    Article Title: UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs
    Article Snippet: For SMARCA4, CHD4 and UTX ChIP–qPCR, 416B cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 10 min at RT. .. For SMARCA4, CHD4 and UTX ChIP–qPCR, 416B cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 10 min at RT.

    Article Title: Signal Transducer and Activator of Transcription 1 Plays a Pivotal Role in RET/PTC3 Oncogene-induced Expression of Indoleamine 2,3-Dioxygenase 1
    Article Snippet: In the first instance, disuccinimidyl glutarate (Sigma) was added to a final concentration of 2 m m and cells were incubated 45 min at room temperature for cross-linking. .. After harvesting, cells were lysed in 700 μl of ice-cold cell lysis buffer (5 m m HEPES, pH 8.0, 85 m m KCl, 0.5% Nonidet P-40, 1 m m PMSF, 1× protease inhibitor mixture (Roche)) for 10 min on ice.

    Article Title: Brg1 Enables Rapid Growth of the Early Embryo by Suppressing Genes That Regulate Apoptosis and Cell Growth Arrest
    Article Snippet: For the CHD4 ChIP, formaldehyde fixation was followed by a 45-min incubation in 2 mM disuccinimidyl glutarate (DSG; catalog number 80424-50MG-F; Sigma). .. The cells were pelleted by centrifugation at 1,350 × g at 40°C in a tabletop centrifuge and resuspended in cold lysis buffer 2 (10 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1× protease inhibitor) and gently rocked at 4°C for 10 min in 14-ml conical tubes.

    Article Title: The WD40-Repeat Proteins NFC101 and NFC102 Regulate Different Aspects of Maize Development through Chromatin Modification
    Article Snippet: Disuccinimidyl glutarate (Sigma-Aldrich) was freshly prepared at 0.5 M in DMSO immediately prior to use and was added to each 50-mL Falcon tube, containing samples in MC buffer, to 2 mM final concentration. .. Disuccinimidyl glutarate (Sigma-Aldrich) was freshly prepared at 0.5 M in DMSO immediately prior to use and was added to each 50-mL Falcon tube, containing samples in MC buffer, to 2 mM final concentration.

    Binding Assay:

    Article Title: Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity
    Article Snippet: The cells were incubated with 2 mM disuccinimidyl glutarate (Sigma-Aldrich) for 25 min at room temperature and then cross-linked with 1% formaldehyde in medium for 10 min. .. The cells were incubated with 2 mM disuccinimidyl glutarate (Sigma-Aldrich) for 25 min at room temperature and then cross-linked with 1% formaldehyde in medium for 10 min.

    Article Title: The WD40-Repeat Proteins NFC101 and NFC102 Regulate Different Aspects of Maize Development through Chromatin Modification
    Article Snippet: To improve fixation of DNA-protein binding for proteins, such as NFC101/NFC102 and Rpd3, which do not bind DNA directly, a two-step fixation technique was adopted ( ). .. Disuccinimidyl glutarate (Sigma-Aldrich) was freshly prepared at 0.5 M in DMSO immediately prior to use and was added to each 50-mL Falcon tube, containing samples in MC buffer, to 2 mM final concentration.

    ChIP-sequencing:

    Article Title: UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs
    Article Snippet: Paragraph title: ChIP-Seq and ChIP-qPCR ... The cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 30 min at 4 °C.

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: Paragraph title: ChIP-Seq of CBFβ in CD8+ TEFF cells and data processing ... The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium.

    Article Title: Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes
    Article Snippet: Paragraph title: T Cell Purification, Chromatin Preparation, ChIP, and ChIP-Seq ... Resting T cells or cells activated using CD3 and CD28 antibody beads ( ) were treated with 2 mM disuccinimidyl glutarate (Sigma) for 45 minute at room temperature to preserve the protein-protein interactions, prior to the cross-linking with 1% formaldehyde at room temperature for 10 minute.

    Article Title: Cell of origin in AML: Susceptibility to MN1-induced transformation is regulated by the MEIS1/AbdB-like HOX protein complex
    Article Snippet: Paragraph title: Chromatin immunoprecipitation (ChIP) assay and ChIP-sequencing ... Chromatin immunoprecipitation assays were performed using sequential cross-linking with disuccinimidyl glutarate (Sigma) and formaldehyde followed by immunoprecipitation (see ) using an anti-HA antibody (Abcam ab9110). qPCR was performed to verify enrichment, and Illumina DNA library construction for next generation sequencing was performed as described previously ( ).

    Isolation:

    Article Title: Chromatin regulation by Brg1 underlies heart muscle development and disease
    Article Snippet: Adult hearts were minced before fixing with 1% PFA (for Brg1 or PARP1 ChIP) or with 1% PFA and 2 mM disuccinimidyl glutarate (Sigma) for HDAC ChIP. .. Cells were lysed by cell lysis buffer (10 mM Hepes pH 7.5, 85 mM KCl, 0.5% NP-40, protease inhibitor (#78410, Pierce, Rockford, IL).

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: hCD2-Cre+ Rosa26GFP Cbfb FL/FL (Cbfb −/− ) naïve CD8+ T cells were isolated by negative selection from uninfected mice. .. The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium.

    Purification:

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium. .. The fixed cells were processed with a truChIP Chromatin Shearing Reagent Kit (Covaris) and sonicated for 5 min on Covaris S2 ultrasonicator.

    Article Title: Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes
    Article Snippet: Paragraph title: T Cell Purification, Chromatin Preparation, ChIP, and ChIP-Seq ... Resting T cells or cells activated using CD3 and CD28 antibody beads ( ) were treated with 2 mM disuccinimidyl glutarate (Sigma) for 45 minute at room temperature to preserve the protein-protein interactions, prior to the cross-linking with 1% formaldehyde at room temperature for 10 minute.

    Sequencing:

    Article Title: Signal Transducer and Activator of Transcription 1 Plays a Pivotal Role in RET/PTC3 Oncogene-induced Expression of Indoleamine 2,3-Dioxygenase 1
    Article Snippet: At variance, for IDO1, 2000 bp of the promoter, localized upstream of the starting codon, and the sequence of the first intron, were analyzed. .. In the first instance, disuccinimidyl glutarate (Sigma) was added to a final concentration of 2 m m and cells were incubated 45 min at room temperature for cross-linking.

    Mouse Assay:

    Article Title: Chromatin regulation by Brg1 underlies heart muscle development and disease
    Article Snippet: Chromatin Immunoprecipitation (ChIP) Hearts from approximately eight litters of E10.5-E11.5 Swiss-Webster mice (80-100 embryonic hearts per ChIP experiment) were dissected in chilled PBS, and subsequently fixed with 1% PFA and washed with 0.125M glycine. .. Adult hearts were minced before fixing with 1% PFA (for Brg1 or PARP1 ChIP) or with 1% PFA and 2 mM disuccinimidyl glutarate (Sigma) for HDAC ChIP.

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: CD45.2+ KLRG1hi IL-7Rαlo CD8+ TEFF cells were sorted from CD45.1+ recipient mice on 8 dpi in adoptive transfer and LCMV-Arm infection experiments. .. The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium.

    Article Title: Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity
    Article Snippet: Total thymocytes from VavCre+ Tle3 −/− mice were used as negative control. .. The cells were incubated with 2 mM disuccinimidyl glutarate (Sigma-Aldrich) for 25 min at room temperature and then cross-linked with 1% formaldehyde in medium for 10 min.

    Chromatin Immunoprecipitation:

    Article Title: Chromatin regulation by Brg1 underlies heart muscle development and disease
    Article Snippet: Chromatin Immunoprecipitation (ChIP) Hearts from approximately eight litters of E10.5-E11.5 Swiss-Webster mice (80-100 embryonic hearts per ChIP experiment) were dissected in chilled PBS, and subsequently fixed with 1% PFA and washed with 0.125M glycine. .. Adult hearts were minced before fixing with 1% PFA (for Brg1 or PARP1 ChIP) or with 1% PFA and 2 mM disuccinimidyl glutarate (Sigma) for HDAC ChIP. .. Cells were lysed by cell lysis buffer (10 mM Hepes pH 7.5, 85 mM KCl, 0.5% NP-40, protease inhibitor (#78410, Pierce, Rockford, IL).

    Article Title: UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs
    Article Snippet: Paragraph title: ChIP-Seq and ChIP-qPCR ... The cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 30 min at 4 °C.

    Article Title: UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs
    Article Snippet: Primer sequences used for ChIP–qPCR are listed in . .. For SMARCA4, CHD4 and UTX ChIP–qPCR, 416B cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 10 min at RT. .. Cross-linking was stopped by addition of 125 mM glycine.

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium. .. The sheared chromatin from 3–5 × 106 CD8+ T cells was immunoprecipitated with 5 μg of purified anti-CBFβ antibody and was washed as described .

    Article Title: Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription
    Article Snippet: Protein–chromatin complexes were crosslinked with 1% paraformaldehyde (PFAH) for 10 min at RT (in case of H3K9me2). .. For JMJD1a, ChIP cells were crosslinked with disuccinimidyl glutarate (Sigma) for 45 min followed by 10 min incubation in 1% PFAH. .. Disuccinimidyl glutarate was used for JMJD1a ChIP since crosslinkers with longer linker arms have been used for JMJD1a ChIP .

    Article Title: Signal Transducer and Activator of Transcription 1 Plays a Pivotal Role in RET/PTC3 Oncogene-induced Expression of Indoleamine 2,3-Dioxygenase 1
    Article Snippet: Paragraph title: Chromatin Immunoprecipitation (ChIP) Assay ... In the first instance, disuccinimidyl glutarate (Sigma) was added to a final concentration of 2 m m and cells were incubated 45 min at room temperature for cross-linking.

    Article Title: MLL1 and MLL1 fusion proteins have distinct functions in regulating leukemic transcription program
    Article Snippet: Paragraph title: Chromatin immunoprecipitation experiment ... After treatments, cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma 80424, St Louis, MO, USA) for 30 min at room temperature.

    Article Title: Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity
    Article Snippet: Paragraph title: ChIP ... The cells were incubated with 2 mM disuccinimidyl glutarate (Sigma-Aldrich) for 25 min at room temperature and then cross-linked with 1% formaldehyde in medium for 10 min.

    Article Title: Brg1 Enables Rapid Growth of the Early Embryo by Suppressing Genes That Regulate Apoptosis and Cell Growth Arrest
    Article Snippet: Cells were cross-linked by adding 13.5 μl of 36.6% formaldehyde (catalog number 8775; Sigma) per 500 μl of sample for 15 min at room temperature. .. For the CHD4 ChIP, formaldehyde fixation was followed by a 45-min incubation in 2 mM disuccinimidyl glutarate (DSG; catalog number 80424-50MG-F; Sigma). .. The cells were lysed in cold lysis buffer 1 (50 mM HEPES-KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1× protease inhibitors) and gently rocked at 4°C for 10 min in 14-ml conical tubes.

    Article Title: The WD40-Repeat Proteins NFC101 and NFC102 Regulate Different Aspects of Maize Development through Chromatin Modification
    Article Snippet: Paragraph title: Assays ... Disuccinimidyl glutarate (Sigma-Aldrich) was freshly prepared at 0.5 M in DMSO immediately prior to use and was added to each 50-mL Falcon tube, containing samples in MC buffer, to 2 mM final concentration.

    Article Title: Ligand Binding Shifts Highly Mobile Retinoid X Receptor to the Chromatin-Bound State in a Coactivator-Dependent Manner, as Revealed by Single-Cell Imaging
    Article Snippet: Paragraph title: ChIP. ... Briefly, cross-linking was carried out by disuccinimidyl glutarate for 30 min and by formaldehyde (Sigma) treatment for 10 min and was followed by RXR immunoprecipitation.

    Article Title: Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes
    Article Snippet: Paragraph title: T Cell Purification, Chromatin Preparation, ChIP, and ChIP-Seq ... Resting T cells or cells activated using CD3 and CD28 antibody beads ( ) were treated with 2 mM disuccinimidyl glutarate (Sigma) for 45 minute at room temperature to preserve the protein-protein interactions, prior to the cross-linking with 1% formaldehyde at room temperature for 10 minute.

    Article Title: Cell of origin in AML: Susceptibility to MN1-induced transformation is regulated by the MEIS1/AbdB-like HOX protein complex
    Article Snippet: The gene set enrichment analysis software ( ) ( ) was used to compare enrichment of curated gene sets (dataset C2) and Gene Ontology gene sets (dataset C5, available from the Molecular Signature database v2.5 ( ). .. Chromatin immunoprecipitation assays were performed using sequential cross-linking with disuccinimidyl glutarate (Sigma) and formaldehyde followed by immunoprecipitation (see ) using an anti-HA antibody (Abcam ab9110). qPCR was performed to verify enrichment, and Illumina DNA library construction for next generation sequencing was performed as described previously ( ). .. Cluster generation and 50bp sequencing on the Illumina Genome Analyzer IIx (Illumina, San Diego, CA) was performed following the manufacturer’s instructions.

    Negative Control:

    Article Title: Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity
    Article Snippet: Total thymocytes from VavCre+ Tle3 −/− mice were used as negative control. .. The cells were incubated with 2 mM disuccinimidyl glutarate (Sigma-Aldrich) for 25 min at room temperature and then cross-linked with 1% formaldehyde in medium for 10 min.

    Selection:

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: hCD2-Cre+ Rosa26GFP Cbfb FL/FL (Cbfb −/− ) naïve CD8+ T cells were isolated by negative selection from uninfected mice. .. The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium.

    Next-Generation Sequencing:

    Article Title: Cell of origin in AML: Susceptibility to MN1-induced transformation is regulated by the MEIS1/AbdB-like HOX protein complex
    Article Snippet: The gene set enrichment analysis software ( ) ( ) was used to compare enrichment of curated gene sets (dataset C2) and Gene Ontology gene sets (dataset C5, available from the Molecular Signature database v2.5 ( ). .. Chromatin immunoprecipitation assays were performed using sequential cross-linking with disuccinimidyl glutarate (Sigma) and formaldehyde followed by immunoprecipitation (see ) using an anti-HA antibody (Abcam ab9110). qPCR was performed to verify enrichment, and Illumina DNA library construction for next generation sequencing was performed as described previously ( ). .. Cluster generation and 50bp sequencing on the Illumina Genome Analyzer IIx (Illumina, San Diego, CA) was performed following the manufacturer’s instructions.

    Immunoprecipitation:

    Article Title: Chromatin regulation by Brg1 underlies heart muscle development and disease
    Article Snippet: Adult hearts were minced before fixing with 1% PFA (for Brg1 or PARP1 ChIP) or with 1% PFA and 2 mM disuccinimidyl glutarate (Sigma) for HDAC ChIP. .. Nuclei were isolated by disruption using a B dounce, and washed with SDS lysis buffer (1% SDS, 10mM EDTA and 50mM Tris, pH 8.1).

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: The cells were incubated with 2 mM disuccinimidyl glutarate (DSG, Sigma Aldrich) at room temperature for 45 min, and then cross-linked for 10 min with 1% formaldehyde in medium. .. The fixed cells were processed with a truChIP Chromatin Shearing Reagent Kit (Covaris) and sonicated for 5 min on Covaris S2 ultrasonicator.

    Article Title: MLL1 and MLL1 fusion proteins have distinct functions in regulating leukemic transcription program
    Article Snippet: After treatments, cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma 80424, St Louis, MO, USA) for 30 min at room temperature. .. Following two washes with 1× phosphate-buffered saline, cells were incubated for 10 min with 1% formaldehyde.

    Article Title: Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity
    Article Snippet: The cells were incubated with 2 mM disuccinimidyl glutarate (Sigma-Aldrich) for 25 min at room temperature and then cross-linked with 1% formaldehyde in medium for 10 min. .. The fixed cells were processed using truChIP Chromatin Shearing Reagent kit (Covaris) and sonicated for 6 min on Covaris S2 ultrasonicator.

    Article Title: Regulator of G Protein Signaling 7 (RGS7) Can Exist in a Homo-oligomeric Form That Is Regulated by Gαo and R7-binding Protein
    Article Snippet: Alternatively, cells were treated with 2 μ m disuccinimidyl glutarate (DSG, Sigma) in PBS for 30 min. .. Alternatively, cells were treated with 2 μ m disuccinimidyl glutarate (DSG, Sigma) in PBS for 30 min.

    Article Title: Ligand Binding Shifts Highly Mobile Retinoid X Receptor to the Chromatin-Bound State in a Coactivator-Dependent Manner, as Revealed by Single-Cell Imaging
    Article Snippet: ChIP was performed as previously described , with minor modifications. .. Briefly, cross-linking was carried out by disuccinimidyl glutarate for 30 min and by formaldehyde (Sigma) treatment for 10 min and was followed by RXR immunoprecipitation. .. After fixation, chromatin was sonicated with a Diagenode Bioruptor to generate 200- to 1,000-bp fragments.

    Article Title: Cell of origin in AML: Susceptibility to MN1-induced transformation is regulated by the MEIS1/AbdB-like HOX protein complex
    Article Snippet: The gene set enrichment analysis software ( ) ( ) was used to compare enrichment of curated gene sets (dataset C2) and Gene Ontology gene sets (dataset C5, available from the Molecular Signature database v2.5 ( ). .. Chromatin immunoprecipitation assays were performed using sequential cross-linking with disuccinimidyl glutarate (Sigma) and formaldehyde followed by immunoprecipitation (see ) using an anti-HA antibody (Abcam ab9110). qPCR was performed to verify enrichment, and Illumina DNA library construction for next generation sequencing was performed as described previously ( ). .. Cluster generation and 50bp sequencing on the Illumina Genome Analyzer IIx (Illumina, San Diego, CA) was performed following the manufacturer’s instructions.

    Lysis:

    Article Title: Chromatin regulation by Brg1 underlies heart muscle development and disease
    Article Snippet: Adult hearts were minced before fixing with 1% PFA (for Brg1 or PARP1 ChIP) or with 1% PFA and 2 mM disuccinimidyl glutarate (Sigma) for HDAC ChIP. .. Cells were lysed by cell lysis buffer (10 mM Hepes pH 7.5, 85 mM KCl, 0.5% NP-40, protease inhibitor (#78410, Pierce, Rockford, IL).

    Article Title: UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs
    Article Snippet: For SMARCA4, CHD4 and UTX ChIP–qPCR, 416B cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 10 min at RT. .. For SMARCA4, CHD4 and UTX ChIP–qPCR, 416B cells were cross-linked with 2 mM disuccinimidyl glutarate (Sigma) for 30 min at RT, and this was followed by a second cross-linking with 1% FA for 10 min at RT.

    Article Title: Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription
    Article Snippet: For JMJD1a, ChIP cells were crosslinked with disuccinimidyl glutarate (Sigma) for 45 min followed by 10 min incubation in 1% PFAH. .. Cells were detached with scraper, collected by centrifugation (300g , 10 min) and washed once with pre-lysis buffer I (10 mM EDTA, 0.5 mM EGTA, 10 mM HEPES and 0.25% Triton X) and once with pre-lysis buffer II (1 mM EDTA, 0.5 mM EGTA, 10 mM HEPES and 100 mM NaCl).

    Article Title: Signal Transducer and Activator of Transcription 1 Plays a Pivotal Role in RET/PTC3 Oncogene-induced Expression of Indoleamine 2,3-Dioxygenase 1
    Article Snippet: In the first instance, disuccinimidyl glutarate (Sigma) was added to a final concentration of 2 m m and cells were incubated 45 min at room temperature for cross-linking. .. Finally, glycine was added to a final concentration of 125 m m and cells incubated for 5 min at room temperature to quench formaldehyde cross-linking.

    Article Title: Brg1 Enables Rapid Growth of the Early Embryo by Suppressing Genes That Regulate Apoptosis and Cell Growth Arrest
    Article Snippet: For the CHD4 ChIP, formaldehyde fixation was followed by a 45-min incubation in 2 mM disuccinimidyl glutarate (DSG; catalog number 80424-50MG-F; Sigma). .. The cells were lysed in cold lysis buffer 1 (50 mM HEPES-KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1× protease inhibitors) and gently rocked at 4°C for 10 min in 14-ml conical tubes.

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    Millipore monoclonal anti caspase 7 antibody
    Monoclonal Anti Caspase 7 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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