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Agilent technologies agilent 2100 bioanalyzer
Agilent 2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 9673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Agilent technologies
Average 99 stars, based on 9673 article reviews
Price from $9.99 to $1999.99
agilent 2100 bioanalyzer - by Bioz Stars, 2020-07
99/100 stars

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RNA Sequencing Assay:

Article Title: Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis)
Article Snippet: .. Reverse transcription PCR In addition to the assessment of RNA quality based on the bacterial and eukaryotic ribosomal RNAs using Agilent Bioanalyzer 2100, one-step RT-PCR was applied to the RNA extracted from microdissection tissues in order to evaluate the quality of bacterial mRNA for RNA-seq analysis. .. The one-step RT-PCR (Qiagen®) was used with specific primers (Additional file : Table S1) targeting three housekeeping genes of L. intracellularis .

Article Title: Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization
Article Snippet: .. RNA-seq analysis Total RNA was isolated from HUVECs, and RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies). .. RNA-seq libraries were constructed using the SENSE mRNA-Seq Library Prep Kit (Lexogen), according to the manufacturer’s instructions, and were sequenced as 100 bp paired-end runs on the HiSeq 2000 platform (Illumina).

Polymerase Chain Reaction:

Article Title: Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis)
Article Snippet: .. Reverse transcription PCR In addition to the assessment of RNA quality based on the bacterial and eukaryotic ribosomal RNAs using Agilent Bioanalyzer 2100, one-step RT-PCR was applied to the RNA extracted from microdissection tissues in order to evaluate the quality of bacterial mRNA for RNA-seq analysis. .. The one-step RT-PCR (Qiagen®) was used with specific primers (Additional file : Table S1) targeting three housekeeping genes of L. intracellularis .

Isolation:

Article Title: Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization
Article Snippet: .. RNA-seq analysis Total RNA was isolated from HUVECs, and RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies). .. RNA-seq libraries were constructed using the SENSE mRNA-Seq Library Prep Kit (Lexogen), according to the manufacturer’s instructions, and were sequenced as 100 bp paired-end runs on the HiSeq 2000 platform (Illumina).

Laser Capture Microdissection:

Article Title: Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis)
Article Snippet: .. Reverse transcription PCR In addition to the assessment of RNA quality based on the bacterial and eukaryotic ribosomal RNAs using Agilent Bioanalyzer 2100, one-step RT-PCR was applied to the RNA extracted from microdissection tissues in order to evaluate the quality of bacterial mRNA for RNA-seq analysis. .. The one-step RT-PCR (Qiagen®) was used with specific primers (Additional file : Table S1) targeting three housekeeping genes of L. intracellularis .

Reverse Transcription Polymerase Chain Reaction:

Article Title: Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis)
Article Snippet: .. Reverse transcription PCR In addition to the assessment of RNA quality based on the bacterial and eukaryotic ribosomal RNAs using Agilent Bioanalyzer 2100, one-step RT-PCR was applied to the RNA extracted from microdissection tissues in order to evaluate the quality of bacterial mRNA for RNA-seq analysis. .. The one-step RT-PCR (Qiagen®) was used with specific primers (Additional file : Table S1) targeting three housekeeping genes of L. intracellularis .

Generated:

Article Title: A qualitative assessment of direct-labeled cDNA products prior to microarray analysis
Article Snippet: .. Fully degraded, partially degraded and intact RNA, generated by titrating varying amounts of RNase, were used to prepare samples for measuring Cy5 incorporation directly into the cDNA by reverse transcription of RNA by the Agilent 2100 Bioanalyzer. .. It should be noted, however, that RNase treatment can be unpredictable, since even slight deviations in temperature and timing of incubation can result in varying degrees of degradation.

Formalin-fixed Paraffin-Embedded:

Article Title: Biobanking: Objectives, Requirements, and Future Challenges—Experiences from the Munich Vascular Biobank
Article Snippet: .. Analysis of RNA Quality from FFPE Biospecimens by RIN and RNA Fragmentation RNA integrity number (RIN) was determined by Agilent 2100 Bioanalyzer and the RNA 6000 Nano Kit (Agilent Technologies, Waldbronn, Germany) in accordance with the manufacturer’s instructions. ..

Sequencing:

Article Title: Decreasing miRNA sequencing bias using a single adapter and circularization approach
Article Snippet: .. Using RealSeq®-AC and TruSeq®, we prepared sequencing libraries from a reference sample of total RNA (Agilent) obtained from nine different human tissues and cell lines. .. We sequenced both libraries to a coverage of ten million reads and counted the number of miRNAs identified (with ten or more reads of each) by each kit at different sequencing coverages by random subsampling (Additional file : Figure S6).

Chromatin Immunoprecipitation:

Article Title: Characterization of human plasma-derived exosomal RNAs by deep sequencing
Article Snippet: .. The quantity and quality of the RNA were determined by Agilent Bioanalyzer 2100 with a Small RNA Chip for exosomal RNA, and a RNA 6000 Pico Kit for cellular RNA (Agilent Technologies, Santa Clara, CA, USA). .. Enzyme protection assay RNA isolated from the plasma exosomes was first incubated at room temperature, either with 30 units/μL of DNase I (QIAGEN) for 10 min or with 10 μg/mL RNase A for 30 min.

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    Agilent technologies disposable rna chips agilent rna 6000 nano labchip kit agilent technologies waldbrunn germany
    Disposable Rna Chips Agilent Rna 6000 Nano Labchip Kit Agilent Technologies Waldbrunn Germany, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/disposable rna chips agilent rna 6000 nano labchip kit agilent technologies waldbrunn germany/product/Agilent technologies
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    disposable rna chips agilent rna 6000 nano labchip kit agilent technologies waldbrunn germany - by Bioz Stars, 2020-07
    88/100 stars
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