dispase ii (Sanko Junyaku Co Ltd)

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Sanko Junyaku Co Ltd dispase ii
Dispase Ii, supplied by Sanko Junyaku Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dispase ii/product/Sanko Junyaku Co Ltd
Average 92 stars, based on 18 article reviews
Price from $9.99 to $1999.99

dispase ii - by Bioz Stars, 2021-01

92/100 stars

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enzymatic digestion
collagenase type iv

Images

Transplantation Assay:

Article Title: Cryopreserved Dental Pulp Tissues of Exfoliated Deciduous Teeth Is a Feasible Stem Cell Resource for Regenerative Medicine
Article Snippet: .. Eight weeks after the transplantation, the primary implants were harvested and treated with 0.4% dispase II (Sanko Junyaku) for 60 min at 37°C to obtain single cells from the implants. .. The cells were stained with R-PE-conjugated anti-human CD146 and magnetic-beads-conjugated anti-R-PE antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) and sorted magnetically to obtain human CD146-positive cells.

Incubation:

Article Title: Production of the medaka derived from vitrified whole testes by germ cell transplantation
Article Snippet: .. Testes of wild-type (WT) medaka were minced and incubated with 0.2% collagenase H (Roche Diagnostics, Mannheim, Germany) and 0.17% dispase II (Sanko Junyaku Co., Ltd., Tokyo, Japan) dissolved in L-15 medium (pH 7.8 with Hepes, Gibco Invitrogen Co., Grand Island NY, USA) containing 10% FBS (Gibco Invitrogen Co.) and 900 U ml−1 DNase I (Roche Diagnostics) for 1 h at 23 °C to prepare the testicular cell suspension. .. The cell suspension was rinsed with L-15 medium containing 10% FBS followed by filtration through a 42-μm pore nylon mesh (NBC Industries) to eliminate non-dissociated cell clumps.

Article Title: Successful Reconstruction of Tooth Germ with Cell Lines Requires Coordinated Gene Expressions from the Initiation Stage
Article Snippet: .. In brief, dental germs of lower first molars were dissected from embryos at day (E) 14.5, 15.5 and 16.5 and incubated in PBS containing 1000 U/mL dispase II (SANKOJUNYAKU CO. LTD., Tokyo, Japan) for 15 min at room temperature, and the epithelia were separated from the mesenchymal tissues with a fine needle. .. Epithelial fractions were treated with 100 U/mL collagenase type I (Worthington Biochemical Co., Lakewood, NJ, ISA) for 20 min at 37 °C.

other:

Article Title: Establishment of a Human Conjunctival Epithelial Cell Line Lacking the Functional Tacstd2 Gene (An American Ophthalmological Society Thesis)
Article Snippet: The conjunctival tissues were then soaked overnight in 1000 PU/mL of dispase (Dispase I; Sanko Junyaku Co, Ltd., Tokyo, Japan) at 4°C.

Article Title: Cryopreserved Dental Pulp Tissues of Exfoliated Deciduous Teeth Is a Feasible Stem Cell Resource for Regenerative Medicine
Article Snippet: Both cryopreserved and fresh dental pulp tissues were digested with 0.3% collagenase type I (Worthington Biochemicals, Lakewood, NJ) and 0.4% dispase II (Sanko Junyaku, Tokyo, Japan) in phosphate buffered saline (PBS, pH 7.4) for 60 min at 37°C.

Article Title: Transplantation of mesenchymal stem cells ameliorates secondary osteoporosis through interleukin-17-impaired functions of recipient bone marrow mesenchymal stem cells in MRL/lpr mice
Article Snippet: The dental pulp tissues of human exfoliated deciduous teeth were minced and then digested with 0.3 % collagenase type I (Worthington Biochemicals, Lakewood, NJ, USA) and 0.4 % dispase II (Sanko Junyaku, Tokyo, Japan) for 60 minutes at 37 °C as reported previously [ ].

Article Title: Dendritic Cells Express Hematopoietic Prostaglandin D Synthase and Function as a Source of Prostaglandin D2 in the Skin
Article Snippet: Keratinocytes were prepared by treating skin tissues with dispase (200 u/ml, Sankojunyaku Co. Ltd., Tokyo, Japan) overnight at 4°C followed by treatment with 0.05% trypsin-EDTA (Invitrogen Corp., Carlsbad, CA) for 10 minutes at 37°C.

Centrifugation:

Article Title: Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells
Article Snippet: .. After centrifugation, the remaining tissue was further treated with D-MEM/F12 1:1 containing 1.67 mg/mL Dispase II (Sanko Junyaku, Tokyo, Japan) for 60 min at 37 °C. .. The cell suspension was passed through a 70-μm Cell Strainer (BD Biosciences, Franklin Lakes, NJ, USA), and centrifuged.