dispase ii solution  (Millipore)


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  • 99
    Name:
    Dispase I
    Description:

    Catalog Number:
    d4818
    Price:
    None
    Applications:
    Dispase I has been used in lung digestion and processing for flow staining, as well as for CD4 cell isolation in mice. The enzyme has also been used to digest excised wounds and a small amount of surrounding skin for the detection of GFP+ (green fluorescence protein) cells. This study to investigated the effect of differentiation and angiogenesis of bone marrow-derived mesenchymal stem cells on wound healing. It has also been used to remove the epidermis during the isolation of dermal fibroblasts from mice.
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    Structured Review

    Millipore dispase ii solution

    https://www.bioz.com/result/dispase ii solution/product/Millipore
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    dispase ii solution - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Orchestrated control of filaggrin–actin scaffolds underpins cornification
    Article Snippet: .. Epidermal sheets were isolated by overnight incubation in dispase (5 U/ml; Sigma-Aldrich, Gillingham, Dorset, UK) and separation of the epidermis from dermal tissues. .. For fluorescent antibody staining, these epidermal sheets were first incubated with 4% formaldehyde (Sigma-Aldrich, Gillingham, Dorset, UK), followed by 0.1% Triton X-100 (Sigma-Aldrich, Gillingham, Dorset, UK).

    Incubation:

    Article Title: Comparison of culture media indicates a role for autologous serum in enhancing phenotypic preservation of rabbit limbal stem cells in explant culture
    Article Snippet: .. After dispase II digestion, epithelial sheets detached from PET inserts were incubated with 0.25% trypsin-EDTA (Sigma) solution at 37 °C for ten min., with shaking at 80 rpm. ..

    Article Title: Orchestrated control of filaggrin–actin scaffolds underpins cornification
    Article Snippet: .. Epidermal sheets were isolated by overnight incubation in dispase (5 U/ml; Sigma-Aldrich, Gillingham, Dorset, UK) and separation of the epidermis from dermal tissues. .. For fluorescent antibody staining, these epidermal sheets were first incubated with 4% formaldehyde (Sigma-Aldrich, Gillingham, Dorset, UK), followed by 0.1% Triton X-100 (Sigma-Aldrich, Gillingham, Dorset, UK).

    Positron Emission Tomography:

    Article Title: Comparison of culture media indicates a role for autologous serum in enhancing phenotypic preservation of rabbit limbal stem cells in explant culture
    Article Snippet: .. After dispase II digestion, epithelial sheets detached from PET inserts were incubated with 0.25% trypsin-EDTA (Sigma) solution at 37 °C for ten min., with shaking at 80 rpm. ..

    Positive Control:

    Article Title: Ocular pathogenesis and immune reaction after intravitreal dispase injection in mice
    Article Snippet: .. Dispase was added as a stimulus at 0.02 U/ml; as a positive control, 2 μg/ml PHA (Fluka; Sigma, Louis, MO) were added in the absence of dispase. .. As a negative control, PBS was added in the absence of dispase.

    Concentration Assay:

    Article Title: A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors
    Article Snippet: .. NSCLC PDEC protocol We used 2692 µl HBSS (Thermo Fisher Scientific, cat. no. 14170112), 187.5 µl of 20 mg ml−1 pronase (Sigma Aldrich, cat. no. 10165921001) to a final concentration of 1,250 µg ml−1 , 27.6 µl of 1 mg ml−1 elastase (Thermo Fisher Scientific, cat. no. NC9301601) to a final concentration of 9.2 µg ml−1 , 30 µl of 10 mg ml−1 DNase I (Sigma Aldrich, cat. no. 11284932001) to a final concentration of 100 µg ml−1 , 30 µl of 10 mg ml−1 Dispase (Sigma Aldrich, cat. no. 4942078001) to a final concentration of 100 µg ml−1 , 30 µl of 150 mg ml−1 collagenase A (Sigma Aldrich, cat. no. 10103578001) to a final concentration of 1,500 µg ml−1 and 3 µl of 100 µg ml−1 collagenase IV (Thermo Fisher Scientific, cat. no. NC9836075) to a final concentration of 1,250 µg ml−1 . .. NSCLC LE protocol We used 4.7 ml RPMI 1640 (Thermo Fisher Scientific, cat. no. 11875093), 200 µl of 2.5 mg ml−1 Liberase TM (Millipore Sigma, cat. no. 5401119001) to a final concentration of 100 µg ml−1 , 50 µl of 10 mg ml−1 DNase I (Sigma Aldrich, cat. no. 11284932001) to a final concentration of 100 µg ml−1 and 46 µl of 1 mg ml−1 elastase (Thermo Fisher Scientific, cat. no. NC9301601) to a final concentration of 9.2 µg ml−1 .

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  • 99
    Millipore dispase ii solution
    Dispase Ii Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dispase ii solution/product/Millipore
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    dispase ii solution - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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