dionex ultimate 3000 rslc nano lc  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dionex ultimate 3000 rslc nano lc
    Dionex Ultimate 3000 Rslc Nano Lc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dionex ultimate 3000 rslc nano lc/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    dionex ultimate 3000 rslc nano lc - by Bioz Stars, 2020-02
    90/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: The complementary role of histology and proteomics for diagnosis and typing of systemic amyloidosis
    Article Snippet: .. Each digested sample was reduced with dithiothreitol (50 μg) at 99 °C for 5 min, freeze dried, reconstituted in 0.1% (v/v) trifluoroacetic acid in HPLC grade water (20 μl) and analysed by HPLC–MSMS, using a Thermo Scientific Q‐Exactive Plus mass spectrometer (Thermo Scientific, Bremen, Germany) coupled to a Dionex Ultimate 3000 RSLC nanoLC (Dionex Softron GmbH, Bavaria, Germany) using a Thermo Easy‐spray Acclaim Pepmap (Fisher Scientific UK Ltd, Loughborough) column (75 μm × 15 cm, 3 μm/100 Å packing), as previously described , . .. MS raw data files were queried using Matrix Science's Mascot search engine ( http://www.matrixscience.com/ ) and the SwissProt database to assign peptide and protein probability scores.

    Flow Cytometry:

    Article Title: Identification of serum proteome signatures of locally advanced and metastatic gastric cancer: a pilot study
    Article Snippet: The analysis was performed on Dionex UltiMate 3000 RSLC nanoLC System connected to Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific); each sample was analyzed separately. .. Tryptic peptides (2.5 µg of peptides) were separated on reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 µm × 25 cm, 2 µm granulation) using the acetonitrile gradient (from 4 to 60 %, in 0.1 % formic acid) at 30 °C and a flow rate of 250 nL/min (for 230 min).

    Article Title: Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels
    Article Snippet: .. Mass Spectrometry Peptides were separated on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) and analysed as described in [ ]. ..

    Article Title: Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development
    Article Snippet: The analysis of phage proteins was performed by using Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) by using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 185 min).

    Article Title: Association of kidney fibrosis with urinary peptides: a path towards non-invasive liquid biopsies?
    Article Snippet: .. Peptide Sequencing To obtain the sequence information, tandem mass spectrometry was performed using a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , . .. Data files were analyzed using Proteome Discoverer 1.2 and were searched against the UniProt human non-redundant database without enzyme specificity and with hydroxylation of proline and lysine, as well as oxidation of methionine.

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: Mass spectrometry analysis Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 230 min).

    Article Title: Identification of Symptomatic Fetuses Infected with Cytomegalovirus Using Amniotic Fluid Peptide Biomarkers
    Article Snippet: .. LC-MS/MS analysis experiments were performed on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly UK). .. For CE-MS/MS, the samples were injected under constant flow and pressure conditions at a pH of 2.2 to ensure that all peptides are positively charged.

    Article Title: Partial-Body Irradiation in Patients with Prostate Cancer Treated with IMRT Has Little Effect on the Composition of Serum Proteome
    Article Snippet: The analysis was performed on Dionex UltiMate 3000 RSLC nanoLC System connected to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA). .. Tryptic peptides were separated on reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation, Thermo Scientific, Waltham, MA, USA) using 240 min acetonitrile gradient (from 4% to 60%, in 0.1% formic acid) at 30 °C and a flow rate of 250 nL∙min−1 .

    Article Title: Optimization of Data-Independent Acquisition Mass Spectrometry for Deep and Highly Sensitive Proteomic Analysis
    Article Snippet: LC-MS/MS Peptides were directly injected onto a 75 μm × 15 cm PicoFrit emitter (New Objective, Woburn, MA, USA) packed in house with C18 core-shell particles (CAPCELL CORE MP 2.7 μm, 160 Å material; Osaka Soda Co., Ltd., Osaka, Japan) and then separated by using an UltiMate 3000 RSLC nanoLC system (Thermo Fisher Scientific). .. In a test of flow rates of 75 to 300 nL/min, peptides were separated by using a 90 min gradient of solvents A (0.1% FA in water) and B (0.1% FA in 80% ACN) comprising 1% B from 0 min and 70% B from 90 min.

    Article Title: Identification of ageing-associated naturally occurring peptides in human urine
    Article Snippet: .. Peptide sequencing For sequencing of peptides the urine samples were analysed on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) as described in [ ]. .. Data files were analysed using Proteome Discoverer 1.2 (activation type: HCD; min-max precursor mass: 790-6,000; precursor mass tolerance: 10 ppm; fragment mass tolerance: 0.05 Da; S/N threshold: 1) and were searched against the Uniprot human non-redundant database without enzyme specificity.

    Article Title: Altered Levels of Proteins and Phosphoproteins, in the Absence of Early Causative Transcriptional Changes, Shape the Molecular Pathogenesis in the Brain of Young Presymptomatic Ki91 SCA3/MJD Mouse
    Article Snippet: Mass Spectrometry Analysis of the Proteome The analysis was performed with the use of Dionex UltiMate 3000 RSLC nanoLC system connected to Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30 °C and a flow rate of 300 nl/min (for 230 min).

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 230 min).

    Article Title: Urinary proteome signature of Renal Cysts and Diabetes syndrome in children
    Article Snippet: .. Briefly, to acquire the sequence information, urine samples were separated on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , . .. Thereafter, data files were examined against the UniProt human non-redundant database using Proteome Discoverer 1.2 (Thermo) and the SEQUEST search engine.

    Sequencing:

    Article Title: Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins
    Article Snippet: After destaining the gel pieces were reduced with DTT (10 mM), alkylated using iodoacetamide (55 mM), dehydrated with 100% acetonitrile and then subjected to overnight digestion at 37 °C with sequencing-grade modified trypsin (Promega, Madison, WI). .. Peptides were separated and analyzed on a Dionex Ultimate 3000 RSLC NanoLC system coupled to a Q-Exactive apparatus (Thermo Fisher, MA).

    Article Title: Association of kidney fibrosis with urinary peptides: a path towards non-invasive liquid biopsies?
    Article Snippet: .. Peptide Sequencing To obtain the sequence information, tandem mass spectrometry was performed using a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , . .. Data files were analyzed using Proteome Discoverer 1.2 and were searched against the UniProt human non-redundant database without enzyme specificity and with hydroxylation of proline and lysine, as well as oxidation of methionine.

    Article Title: Identification of Symptomatic Fetuses Infected with Cytomegalovirus Using Amniotic Fluid Peptide Biomarkers
    Article Snippet: Paragraph title: Sequencing of peptide biomarkers ... LC-MS/MS analysis experiments were performed on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly UK).

    Article Title: Identification of ageing-associated naturally occurring peptides in human urine
    Article Snippet: .. Peptide sequencing For sequencing of peptides the urine samples were analysed on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) as described in [ ]. .. Data files were analysed using Proteome Discoverer 1.2 (activation type: HCD; min-max precursor mass: 790-6,000; precursor mass tolerance: 10 ppm; fragment mass tolerance: 0.05 Da; S/N threshold: 1) and were searched against the Uniprot human non-redundant database without enzyme specificity.

    Article Title: Urinary proteome signature of Renal Cysts and Diabetes syndrome in children
    Article Snippet: .. Briefly, to acquire the sequence information, urine samples were separated on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , . .. Thereafter, data files were examined against the UniProt human non-redundant database using Proteome Discoverer 1.2 (Thermo) and the SEQUEST search engine.

    In Vitro:

    Article Title: Proteolytic signatures define unique thrombin-derived peptides present in human wound fluid in vivo
    Article Snippet: LC-MS/MS and data analysis After in vitro enzymatic digestion of thrombin, the reaction was stopped by acidifying the samples. .. Peptides were separated and analysed on a Dionex Ultimate 3000 RSLC nanoLC system coupled to a Q-Exactive tandem mass spectrometer (Thermo Fisher, MA).

    Fluorescence:

    Article Title: The complementary role of histology and proteomics for diagnosis and typing of systemic amyloidosis
    Article Snippet: In tissue samples where CR staining was equivocal or scanty, usually as a result of the presence of areas of amorphous eosinophilic material under brightfield light with seemingly white birefringence but bright fluorescence, such areas were captured by the same method. .. Each digested sample was reduced with dithiothreitol (50 μg) at 99 °C for 5 min, freeze dried, reconstituted in 0.1% (v/v) trifluoroacetic acid in HPLC grade water (20 μl) and analysed by HPLC–MSMS, using a Thermo Scientific Q‐Exactive Plus mass spectrometer (Thermo Scientific, Bremen, Germany) coupled to a Dionex Ultimate 3000 RSLC nanoLC (Dionex Softron GmbH, Bavaria, Germany) using a Thermo Easy‐spray Acclaim Pepmap (Fisher Scientific UK Ltd, Loughborough) column (75 μm × 15 cm, 3 μm/100 Å packing), as previously described , .

    Multiple Displacement Amplification:

    Article Title: Association of kidney fibrosis with urinary peptides: a path towards non-invasive liquid biopsies?
    Article Snippet: Peptide Sequencing To obtain the sequence information, tandem mass spectrometry was performed using a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , . .. Peptides were accepted only if they had a mass deviation below ±5 ppm and < 50 mDa for the fragment ions.

    Laser Capture Microdissection:

    Article Title: The complementary role of histology and proteomics for diagnosis and typing of systemic amyloidosis
    Article Snippet: Paragraph title: Laser capture microdissection and proteomic mass spectrometry analysis ... Each digested sample was reduced with dithiothreitol (50 μg) at 99 °C for 5 min, freeze dried, reconstituted in 0.1% (v/v) trifluoroacetic acid in HPLC grade water (20 μl) and analysed by HPLC–MSMS, using a Thermo Scientific Q‐Exactive Plus mass spectrometer (Thermo Scientific, Bremen, Germany) coupled to a Dionex Ultimate 3000 RSLC nanoLC (Dionex Softron GmbH, Bavaria, Germany) using a Thermo Easy‐spray Acclaim Pepmap (Fisher Scientific UK Ltd, Loughborough) column (75 μm × 15 cm, 3 μm/100 Å packing), as previously described , .

    Microscopy:

    Article Title: The complementary role of histology and proteomics for diagnosis and typing of systemic amyloidosis
    Article Snippet: Laser capture microdissection and proteomic mass spectrometry analysis For microdissection, stained CR sections were viewed under brightfield and florescence light using the Leica LMD7 laser capture microscope. .. Each digested sample was reduced with dithiothreitol (50 μg) at 99 °C for 5 min, freeze dried, reconstituted in 0.1% (v/v) trifluoroacetic acid in HPLC grade water (20 μl) and analysed by HPLC–MSMS, using a Thermo Scientific Q‐Exactive Plus mass spectrometer (Thermo Scientific, Bremen, Germany) coupled to a Dionex Ultimate 3000 RSLC nanoLC (Dionex Softron GmbH, Bavaria, Germany) using a Thermo Easy‐spray Acclaim Pepmap (Fisher Scientific UK Ltd, Loughborough) column (75 μm × 15 cm, 3 μm/100 Å packing), as previously described , .

    Activation Assay:

    Article Title: Identification of Symptomatic Fetuses Infected with Cytomegalovirus Using Amniotic Fluid Peptide Biomarkers
    Article Snippet: LC-MS/MS analysis experiments were performed on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly UK). .. All resultant MS/MS data were analysed using Proteome Discoverer 1.2 (activation type: HCD; min-max precursor mass: 790–6000; precursor mass tolerance: 10 ppm; fragment mass tolerance: 0.05 Da; S/N threshold: 1) and were searched against the Uniprot human non-redundant database without enzyme specificity.

    Article Title: Identification of ageing-associated naturally occurring peptides in human urine
    Article Snippet: Peptide sequencing For sequencing of peptides the urine samples were analysed on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) as described in [ ]. .. Data files were analysed using Proteome Discoverer 1.2 (activation type: HCD; min-max precursor mass: 790-6,000; precursor mass tolerance: 10 ppm; fragment mass tolerance: 0.05 Da; S/N threshold: 1) and were searched against the Uniprot human non-redundant database without enzyme specificity.

    Quantitation Assay:

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: .. Mass spectrometry analysis Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 230 min).

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: .. Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 230 min).

    Migration:

    Article Title: Urinary proteome signature of Renal Cysts and Diabetes syndrome in children
    Article Snippet: Briefly, to acquire the sequence information, urine samples were separated on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , . .. The matching of the peptide sequence obtained by MS/MS analysis to the CE-MS peaks was based on molecular mass [Da] and theoretical migration time, calculated using the number of basic amino acids .

    Mass Spectrometry:

    Article Title: Identification of serum proteome signatures of locally advanced and metastatic gastric cancer: a pilot study
    Article Snippet: .. The analysis was performed on Dionex UltiMate 3000 RSLC nanoLC System connected to Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific); each sample was analyzed separately. .. Tryptic peptides (2.5 µg of peptides) were separated on reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 µm × 25 cm, 2 µm granulation) using the acetonitrile gradient (from 4 to 60 %, in 0.1 % formic acid) at 30 °C and a flow rate of 250 nL/min (for 230 min).

    Article Title: Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels
    Article Snippet: .. Mass Spectrometry Peptides were separated on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) and analysed as described in [ ]. ..

    Article Title: Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development
    Article Snippet: .. The analysis of phage proteins was performed by using Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) by using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 185 min).

    Article Title: Association of kidney fibrosis with urinary peptides: a path towards non-invasive liquid biopsies?
    Article Snippet: .. Peptide Sequencing To obtain the sequence information, tandem mass spectrometry was performed using a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , . .. Data files were analyzed using Proteome Discoverer 1.2 and were searched against the UniProt human non-redundant database without enzyme specificity and with hydroxylation of proline and lysine, as well as oxidation of methionine.

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: .. Mass spectrometry analysis Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 230 min).

    Article Title: Partial-Body Irradiation in Patients with Prostate Cancer Treated with IMRT Has Little Effect on the Composition of Serum Proteome
    Article Snippet: .. The analysis was performed on Dionex UltiMate 3000 RSLC nanoLC System connected to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA). .. Tryptic peptides were separated on reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation, Thermo Scientific, Waltham, MA, USA) using 240 min acetonitrile gradient (from 4% to 60%, in 0.1% formic acid) at 30 °C and a flow rate of 250 nL∙min−1 .

    Article Title: Proteolytic signatures define unique thrombin-derived peptides present in human wound fluid in vivo
    Article Snippet: .. Peptides were separated and analysed on a Dionex Ultimate 3000 RSLC nanoLC system coupled to a Q-Exactive tandem mass spectrometer (Thermo Fisher, MA). .. Five μl of sample was injected into an acclaim peptide trap column via the auto-sampler of the Dionex RSLC nanoLC system.

    Article Title: Identification of ageing-associated naturally occurring peptides in human urine
    Article Snippet: .. Peptide sequencing For sequencing of peptides the urine samples were analysed on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) as described in [ ]. .. Data files were analysed using Proteome Discoverer 1.2 (activation type: HCD; min-max precursor mass: 790-6,000; precursor mass tolerance: 10 ppm; fragment mass tolerance: 0.05 Da; S/N threshold: 1) and were searched against the Uniprot human non-redundant database without enzyme specificity.

    Article Title: Altered Levels of Proteins and Phosphoproteins, in the Absence of Early Causative Transcriptional Changes, Shape the Molecular Pathogenesis in the Brain of Young Presymptomatic Ki91 SCA3/MJD Mouse
    Article Snippet: .. Mass Spectrometry Analysis of the Proteome The analysis was performed with the use of Dionex UltiMate 3000 RSLC nanoLC system connected to Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30 °C and a flow rate of 300 nl/min (for 230 min).

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: .. Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 230 min).

    Article Title: The complementary role of histology and proteomics for diagnosis and typing of systemic amyloidosis
    Article Snippet: .. Each digested sample was reduced with dithiothreitol (50 μg) at 99 °C for 5 min, freeze dried, reconstituted in 0.1% (v/v) trifluoroacetic acid in HPLC grade water (20 μl) and analysed by HPLC–MSMS, using a Thermo Scientific Q‐Exactive Plus mass spectrometer (Thermo Scientific, Bremen, Germany) coupled to a Dionex Ultimate 3000 RSLC nanoLC (Dionex Softron GmbH, Bavaria, Germany) using a Thermo Easy‐spray Acclaim Pepmap (Fisher Scientific UK Ltd, Loughborough) column (75 μm × 15 cm, 3 μm/100 Å packing), as previously described , . .. MS raw data files were queried using Matrix Science's Mascot search engine ( http://www.matrixscience.com/ ) and the SwissProt database to assign peptide and protein probability scores.

    Article Title: Urinary proteome signature of Renal Cysts and Diabetes syndrome in children
    Article Snippet: .. Briefly, to acquire the sequence information, urine samples were separated on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , . .. Thereafter, data files were examined against the UniProt human non-redundant database using Proteome Discoverer 1.2 (Thermo) and the SEQUEST search engine.

    Tandem Mass Spectroscopy:

    Article Title: Identification of serum proteome signatures of locally advanced and metastatic gastric cancer: a pilot study
    Article Snippet: The analysis was performed on Dionex UltiMate 3000 RSLC nanoLC System connected to Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific); each sample was analyzed separately. .. The spectrometer was operating in the data-dependent MS/MS mode with survey scans acquired at a resolution of 70,000 at m/z 200 Da in MS mode and 17,500 at m/z 200 Da in MS2 mode, respectively.

    Article Title: Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development
    Article Snippet: The analysis of phage proteins was performed by using Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Mass spectra were acquired on the Q Exactive in a data-dependent mode by using top 10 data-dependent MS/MS scans.

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: Mass spectrometry analysis Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. The spectrometer was operated in data-dependent MS/MS mode with survey scans acquired at a resolution of 70,000 at m/z 200 in MS mode, and 17,500 at m/z 200 in MS2 mode.

    Article Title: Identification of Symptomatic Fetuses Infected with Cytomegalovirus Using Amniotic Fluid Peptide Biomarkers
    Article Snippet: LC-MS/MS analysis experiments were performed on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly UK). .. Both, CE and LC, were directly interfaced with an LTQ-Orbitrap XL (Thermo Finnigan, Bremen, Germany), using data-dependent HCD MS/MS sequencing of a maximum of the top 20 ions.

    Article Title: Partial-Body Irradiation in Patients with Prostate Cancer Treated with IMRT Has Little Effect on the Composition of Serum Proteome
    Article Snippet: The analysis was performed on Dionex UltiMate 3000 RSLC nanoLC System connected to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA). .. The spectrometer was operating in the data-dependent MS/MS mode with survey scans acquired at a resolution of 70,000 at m/z 200 Da in MS mode and 17,500 at m/z 200 Da in MS2 mode, respectively.

    Article Title: Altered Levels of Proteins and Phosphoproteins, in the Absence of Early Causative Transcriptional Changes, Shape the Molecular Pathogenesis in the Brain of Young Presymptomatic Ki91 SCA3/MJD Mouse
    Article Snippet: Mass Spectrometry Analysis of the Proteome The analysis was performed with the use of Dionex UltiMate 3000 RSLC nanoLC system connected to Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. The spectrometer was operated in data-dependent MS/MS mode (tandem mass spectrometry) with survey scans acquired at a resolution of 70,000 at m /z 200 in MS mode and 17,500 at m /z 200 in MS2 mode.

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. The spectrometer was operated in data-dependent MS/MS mode with survey scans acquired at a resolution of 70,000 at m/z 200 in MS mode, and 17,500 at m/z 200 in MS2 mode.

    Article Title: Urinary proteome signature of Renal Cysts and Diabetes syndrome in children
    Article Snippet: Peptide sequencing Candidate peptides for the RCAD-classifier were identified and sequenced by the use of tandem mass spectrometry (MS/MS) analysis and searched against human entries in the UniProt database, as previously described , . .. Briefly, to acquire the sequence information, urine samples were separated on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , .

    Modification:

    Article Title: Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins
    Article Snippet: After destaining the gel pieces were reduced with DTT (10 mM), alkylated using iodoacetamide (55 mM), dehydrated with 100% acetonitrile and then subjected to overnight digestion at 37 °C with sequencing-grade modified trypsin (Promega, Madison, WI). .. Peptides were separated and analyzed on a Dionex Ultimate 3000 RSLC NanoLC system coupled to a Q-Exactive apparatus (Thermo Fisher, MA).

    Article Title: Urinary proteome signature of Renal Cysts and Diabetes syndrome in children
    Article Snippet: Briefly, to acquire the sequence information, urine samples were separated on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly, UK) or a Beckman CE systems (PACE MDQ) coupled to an Orbitrap Velos MS instrument (Thermo Fisher Scientific) , . .. No fixed modifications were selected, hydroxylation of proline and lysine and oxidation of methionine were enabled as an optional modification, no enzyme specificity was specified in the settings .

    Sonication:

    Article Title: The complementary role of histology and proteomics for diagnosis and typing of systemic amyloidosis
    Article Snippet: Proteins were extracted from each sample into 10 mM Tris/1 mm EDTA/0.002% Zwittergent buffer solution (35 μl) by heating (99 °C, 1.5 h) followed by sonication (1 h) and then digested with trypsin (25 ng/sample) overnight (~18 h) at 37 °C. .. Each digested sample was reduced with dithiothreitol (50 μg) at 99 °C for 5 min, freeze dried, reconstituted in 0.1% (v/v) trifluoroacetic acid in HPLC grade water (20 μl) and analysed by HPLC–MSMS, using a Thermo Scientific Q‐Exactive Plus mass spectrometer (Thermo Scientific, Bremen, Germany) coupled to a Dionex Ultimate 3000 RSLC nanoLC (Dionex Softron GmbH, Bavaria, Germany) using a Thermo Easy‐spray Acclaim Pepmap (Fisher Scientific UK Ltd, Loughborough) column (75 μm × 15 cm, 3 μm/100 Å packing), as previously described , .

    Injection:

    Article Title: Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins
    Article Snippet: Peptides were separated and analyzed on a Dionex Ultimate 3000 RSLC NanoLC system coupled to a Q-Exactive apparatus (Thermo Fisher, MA). .. Approximately 1.5 µg of material was injected into an Acclaim peptide trap column via the autosampler of a Dionex RSLC NanoLC system.

    Article Title: Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development
    Article Snippet: The analysis of phage proteins was performed by using Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Target value for the full scan MS spectra was set to 1e6 with a maximum injection time of 100 ms and a resolution of 70,000 at m/z 400.

    Article Title: Identification of Symptomatic Fetuses Infected with Cytomegalovirus Using Amniotic Fluid Peptide Biomarkers
    Article Snippet: LC-MS/MS analysis experiments were performed on a Dionex Ultimate 3000 RSLC nano flow system (Dionex, Camberly UK). .. For CE-MS/MS, the samples were injected under constant flow and pressure conditions at a pH of 2.2 to ensure that all peptides are positively charged.

    Article Title: Proteolytic signatures define unique thrombin-derived peptides present in human wound fluid in vivo
    Article Snippet: Peptides were separated and analysed on a Dionex Ultimate 3000 RSLC nanoLC system coupled to a Q-Exactive tandem mass spectrometer (Thermo Fisher, MA). .. Five μl of sample was injected into an acclaim peptide trap column via the auto-sampler of the Dionex RSLC nanoLC system.

    Article Title: Optimization of Data-Independent Acquisition Mass Spectrometry for Deep and Highly Sensitive Proteomic Analysis
    Article Snippet: .. LC-MS/MS Peptides were directly injected onto a 75 μm × 15 cm PicoFrit emitter (New Objective, Woburn, MA, USA) packed in house with C18 core-shell particles (CAPCELL CORE MP 2.7 μm, 160 Å material; Osaka Soda Co., Ltd., Osaka, Japan) and then separated by using an UltiMate 3000 RSLC nanoLC system (Thermo Fisher Scientific). .. In a test of flow rates of 75 to 300 nL/min, peptides were separated by using a 90 min gradient of solvents A (0.1% FA in water) and B (0.1% FA in 80% ACN) comprising 1% B from 0 min and 70% B from 90 min.

    Derivative Assay:

    Article Title: Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development
    Article Snippet: The analysis of phage proteins was performed by using Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) by using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 185 min).

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: Mass spectrometry analysis Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 230 min).

    Article Title: Altered Levels of Proteins and Phosphoproteins, in the Absence of Early Causative Transcriptional Changes, Shape the Molecular Pathogenesis in the Brain of Young Presymptomatic Ki91 SCA3/MJD Mouse
    Article Snippet: Mass Spectrometry Analysis of the Proteome The analysis was performed with the use of Dionex UltiMate 3000 RSLC nanoLC system connected to Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30 °C and a flow rate of 300 nl/min (for 230 min).

    Article Title: Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis
    Article Snippet: Identification and quantitation of exosome proteins were performed using the Dionex UltiMate 3000 RSLC nanoLC System connected to QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). .. Peptides derived from in-solution digestion were separated on a reverse phase Acclaim PepMap RSLC nanoViper C18 column (75 μm × 25 cm, 2 μm granulation) using acetonitrile gradient (from 4 to 60%, in 0.1% formic acid) at 30°C and a flow rate of 300 nL/min (for 230 min).

    Staining:

    Article Title: Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins
    Article Snippet: The gel was stained with Coomassie brilliant blue. .. Peptides were separated and analyzed on a Dionex Ultimate 3000 RSLC NanoLC system coupled to a Q-Exactive apparatus (Thermo Fisher, MA).

    Article Title: The complementary role of histology and proteomics for diagnosis and typing of systemic amyloidosis
    Article Snippet: In renal tissues in which the differential diagnosis included amyloid or non‐amyloid monoclonal gammopathy of renal significance (MGRS), and in which presence of amyloid was not confirmed by CR staining, glomeruli were captured by the same method. .. Each digested sample was reduced with dithiothreitol (50 μg) at 99 °C for 5 min, freeze dried, reconstituted in 0.1% (v/v) trifluoroacetic acid in HPLC grade water (20 μl) and analysed by HPLC–MSMS, using a Thermo Scientific Q‐Exactive Plus mass spectrometer (Thermo Scientific, Bremen, Germany) coupled to a Dionex Ultimate 3000 RSLC nanoLC (Dionex Softron GmbH, Bavaria, Germany) using a Thermo Easy‐spray Acclaim Pepmap (Fisher Scientific UK Ltd, Loughborough) column (75 μm × 15 cm, 3 μm/100 Å packing), as previously described , .

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  • 90
    Thermo Fisher ultimate 3000 rslc nano lc system
    Ultimate 3000 Rslc Nano Lc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 8 article reviews
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    ultimate 3000 rslc nano lc system - by Bioz Stars, 2020-02
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