# dioctanoyl phosphatidylinositol 4 5 bisphosphate (Echelon Biosciences)

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## Structured Review

Dioctanoyl Phosphatidylinositol 4 5 Bisphosphate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

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Average 86 stars, based on 1 article reviews

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## Images

### 1) Product Images from "Involvement of TRPC5 Channels, Inwardly Rectifying K + channels, PLCβ and PIP 2 in Vasopressin-mediated Excitation of Medial Central Amygdala Neurons"

**Article Title: **Involvement of TRPC5 Channels, Inwardly Rectifying K + channels, PLCβ and PIP 2 in Vasopressin-mediated Excitation of Medial Central Amygdala Neurons

**Journal: **The Journal of physiology

**doi: **10.1113/JP281260

**Figure Legend Snippet:**Aa-d, Intracellular perfusion of diC8-PIP2 blocked AVP-induced increases in AP firing of rat CeM neurons evoked by injections of positive currents from 30 pA to 330 pA at an increment of 30 pA every 10 s. Aa, APs recorded by the protocol before (left) and during (right) the application of AVP from a rat CeM neuron dialyzed with diC8-PIP2 (20 μM). Ab, Relationship between the injected currents and the elicited AP numbers from 13 CeM neurons perfused with diC8-PIP2 via the recording pipettes (F(1,12) = 4.38, P = 0.058, Two-way repeated measures ANOVA followed by Sidak multiple comparison test). Ac, Co-plot of the basal relationship of current injected and the number of APs elicited in control condition (data pooled from 13 LTB neurons and 7 RS neurons in Figure 2) and in the cells dialyzed with diC8-PIP2 prior to AVP application (F(1,341) = 20.84, P < 0.0001, Ordinary Two-way ANOVA followed by Sidak multiple comparison test. P > 0.05 for every pairwise comparison between control and diC8-PIP2 at each current injected). Ad, Bath application of QO-58 (15 μM), a Kv7 channel opener significantly decreased the number of APs in rat CeM cells dialyzed with diC8-PIP2 (n = (12, 4), Two-way repeated measures ANOVA followed by Sidak multiple comparison test, F(1,11) = 137.7, P < 0.0001; * P = 0.021, ** P < 0.0001 for the pairwise comparison at the currents injected between control and QO-58). Following application of AVP in the presence of both diC8-PIP2 and QO-58 failed to increase AP numbers further (F(1,11) = 2.23, P = 0.164, Two-way repeated measures ANOVA followed by Sidak multiple comparison test). Ba-f, AVP increased the numbers of APs elicited by injections of the positive currents in CeM neurons of WT mice, but failed to enhance significantly those of TRPC5 KO mice. Ba, APs recorded by the current injection protocol before (left) and during (right) the application of AVP from a WT CeM neuron. Bb, Relationship between the injected currents and the elicited AP numbers from 15 cells of 5 WT mice (Two-way repeated measures ANOVA followed by Sidak multiple comparison test, F(1,14) = 45.5, P < 0.0001, ** P < 0.0001 for the pairwise comparison at each current injected). Bc, APs recorded by the current injection protocol before (left) and during (right) the application of AVP from a TRPC5 KO CeM neuron. Bd, Relationship between the injected currents and the elicited AP numbers from 15 cells from 5 TRPC5 KO mice (F(1,14) = 2.46, P = 0.139, Two-way repeated measures ANOVA followed by Sidak multiple comparison test). Be, Co-plots of the relationship of current injected and the number of APs elicited in control condition prior to AVP application in the CeM neurons from WT mice (n = (15, 5)) and TRPC5 KO mice (n = (15, 5)) (Ordinary Two-way ANOVA followed by Sidak multiple comparison test, F(1,308) = 137.6, P < 0.0001, * P < 0.05, **P < 0.01 for the pairwise comparisons indicated at the currents injected). Bf, Bath application of QO-58 (20 μM) significantly lowered the number of APs in TRPC5 KO CeM neurons (n = (12, 4), Two-way repeated measures ANOVA followed by Sidak multiple comparison test, F(1,11) = 153, P < 0.0001, ** P < 0.0001 for the pairwise comparisons at the indicated currents injected between control and QO-58). Following application of AVP failed to enhance AP firing numbers significantly in TRPC5 KO CeM neurons (n = (12, 4), F(1,11) = 0.78, P = 0.397, Two-way repeated measures ANOVA followed by Sidak multiple comparison test). Ca-b, Depression of Kir channels contributed partially to AP-elicited facilitation of AP firing numbers in rat CeM neurons. Ca, APs recorded from a rat CeM neuron evoked by the current injection protocol before (left) and during (right) the application of AVP in the continuous presence of M084 (100 μM) to block TRPC5 channels. Note that AVP increased AP numbers in response to injections of lower current intensities. Cb, Relationship between the injected currents and the elicited AP numbers from 15 CeM cells of 4 rats prior to and during the application of AVP in the continuous presence of M084. Note that there was significant difference for the AP numbers when the injection currents were from 90 to 150 pA (Two-way repeated measures ANOVA followed by Sidak multiple comparison test, Drug: F(1,14) = 3.06, P = 0.102; Current: F(10,140) = 188.2, P < 0.0001; Drug x Current: F(10,140) = 2.432, P = 0.011; * P = 0.034 at 90 pA, ** P = 0.0007 at 120 pA, * P = 0.017 at 150 pA).

**Techniques Used: **Injection, Blocking Assay

**Figure Legend Snippet:**A, Intracellular dialysis of IP3 receptor blocker, heparin (2 mg/ml), did not block AVP-induced depolarization. B, Intracellular dialysis of the sarco-endoplasmic reticulum Ca2+-ATPases inhibitor, thapsigargin (10 μM) failed to block AVP-elicited depolarization. C, Pretreatment of slices with and continuous bath application of the selective PKC inhibitor, Bis II (1 μM), did not block AVP-mediated depolarization. D, Pretreatment of slices with and continuous bath application of the selective PKC inhibitor, chelerythrine (10 μM), failed to block AVP-mediated depolarization. E, Intracellular dialysis of diC8-PIP2 (20 μM) blocked AVP-induced depolarization. F, Summary data. ** P = 0.008 vs. AVP alone (One-way ANOVA followed by Dunnett’s multiple comparison test).

**Techniques Used: **Blocking Assay