dimethylthiourea  (Millipore)

 
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    Name:
    N N Dimethylthiourea
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    Catalog Number:
    D188700
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    Structured Review

    Millipore dimethylthiourea
    N N Dimethylthiourea

    https://www.bioz.com/result/dimethylthiourea/product/Millipore
    Average 93 stars, based on 1 article reviews
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    dimethylthiourea - by Bioz Stars, 2021-04
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    Images

    1) Product Images from "Ethylene and hydrogen peroxide are involved in brassinosteroid-induced salt tolerance in tomato"

    Article Title: Ethylene and hydrogen peroxide are involved in brassinosteroid-induced salt tolerance in tomato

    Journal: Scientific Reports

    doi: 10.1038/srep35392

    Changes of ethylene emission ( A ), ACC content ( B ), ACS activity ( C ) and ACO activity ( D ) in H 2 O 2 scavenger DMTU or inhibitor DPI pre-treated plants as influenced by 0.1 μM BL. Tomato plants were treated with 5 mM DMTU for 8 h and then treated with 0.1 μM BL for another 12 h. Single treatment of BL or DMTU/DPI was included as control.Changes in ethylene emission ( E ) ACC content ( F ), ACS activity ( G ) and ACO activity ( H ) in SlBRI1 -, SlBAK1 - and SlDWARF -silenced plants as influenced by 0.1 μM BL. Involvement of H 2 O 2 in the BR-induced ethylene accumulation. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P
    Figure Legend Snippet: Changes of ethylene emission ( A ), ACC content ( B ), ACS activity ( C ) and ACO activity ( D ) in H 2 O 2 scavenger DMTU or inhibitor DPI pre-treated plants as influenced by 0.1 μM BL. Tomato plants were treated with 5 mM DMTU for 8 h and then treated with 0.1 μM BL for another 12 h. Single treatment of BL or DMTU/DPI was included as control.Changes in ethylene emission ( E ) ACC content ( F ), ACS activity ( G ) and ACO activity ( H ) in SlBRI1 -, SlBAK1 - and SlDWARF -silenced plants as influenced by 0.1 μM BL. Involvement of H 2 O 2 in the BR-induced ethylene accumulation. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences (P

    Techniques Used: Activity Assay, Standard Deviation

    2) Product Images from "The neurotoxic effects of hydrogen peroxide and copper in Retzius nerve cells of the leech Haemopis sanguisuga"

    Article Title: The neurotoxic effects of hydrogen peroxide and copper in Retzius nerve cells of the leech Haemopis sanguisuga

    Journal: Biology Open

    doi: 10.1242/bio.014936

    The effects of the antioxidants on the duration of Retzius nerve cells' action potentials prolonged by H 2 O 2 /Cu(II). The neurotoxic effect of H 2 O 2 /Cu(II) on spontaneous spike electrogenesis of the Retzius neurons was reduced in the presence of the dimethylthiourea (1 mM) and dimethyl sulfoxide (1%), but not the mannitol (5 mM). The measures are expressed as mean±s.d.; * P
    Figure Legend Snippet: The effects of the antioxidants on the duration of Retzius nerve cells' action potentials prolonged by H 2 O 2 /Cu(II). The neurotoxic effect of H 2 O 2 /Cu(II) on spontaneous spike electrogenesis of the Retzius neurons was reduced in the presence of the dimethylthiourea (1 mM) and dimethyl sulfoxide (1%), but not the mannitol (5 mM). The measures are expressed as mean±s.d.; * P

    Techniques Used:

    3) Product Images from "Complement-dependent Proinflammatory Properties of the Alzheimer's Disease ?-Peptide "

    Article Title: Complement-dependent Proinflammatory Properties of the Alzheimer's Disease ?-Peptide

    Journal: The Journal of Experimental Medicine

    doi:

    Assessment of bonds mediating binding of Aβ to C3 activation fragments. ( a ) Preaggregated Aβ 1–42 (25 μM) was incubated in NHS, and complexes were captured on 10D5-coated wells; NHS only does not contain Aβ. Replicate samples were treated with pH 9.5 buffer, or with the same buffer containing 1 M hydroxylamine ( NH 2 OH ), and remaining bound C3 was then detected and quantitated as described in Materials and Methods. The control containing Aβ, NHS, and EDTA has been subtracted. ( b ) Replicate wells subjected to treatment with the pH 9.5 buffer or hydroxylamine were evaluated for residual bound Aβ as described in Materials and Methods. ( c ) Preaggregated Aβ 1–40 was incubated in NHS in the presence or absence of EDTA; lane 5 contains NHS but no Aβ. After centrifugation and washing, fibrillar Aβ pellets were incubated with pH 7.4 buffer (lanes 1 and 2 ), pH 9.5 buffer (lane 3 ), or 1 M hydroxylamine in pH 9.5 buffer (lane 4 ). After further washing, samples were subjected to SDS-PAGE under nonreducing conditions followed by blotting for the presence of C3 and, after stripping, for Aβ. Arrow , The C3 band at ∼180 kD. ( d ) Preaggregated Aβ 1–42 (50 μM) was incubated in NHS alone, and in the presence of deferoxamine ( Defer. ; 1 mM), glutathione ( Glut. ; 1 mM), dimethylthiourea ( DMTU ; 30 mM), catalase ( CAT ; 2 × 10 4 U/ml), SOD (10 μM), or catalase plus SOD, and the Aβ complexes with C3 activation fragments were then detected as described in Materials and Methods.
    Figure Legend Snippet: Assessment of bonds mediating binding of Aβ to C3 activation fragments. ( a ) Preaggregated Aβ 1–42 (25 μM) was incubated in NHS, and complexes were captured on 10D5-coated wells; NHS only does not contain Aβ. Replicate samples were treated with pH 9.5 buffer, or with the same buffer containing 1 M hydroxylamine ( NH 2 OH ), and remaining bound C3 was then detected and quantitated as described in Materials and Methods. The control containing Aβ, NHS, and EDTA has been subtracted. ( b ) Replicate wells subjected to treatment with the pH 9.5 buffer or hydroxylamine were evaluated for residual bound Aβ as described in Materials and Methods. ( c ) Preaggregated Aβ 1–40 was incubated in NHS in the presence or absence of EDTA; lane 5 contains NHS but no Aβ. After centrifugation and washing, fibrillar Aβ pellets were incubated with pH 7.4 buffer (lanes 1 and 2 ), pH 9.5 buffer (lane 3 ), or 1 M hydroxylamine in pH 9.5 buffer (lane 4 ). After further washing, samples were subjected to SDS-PAGE under nonreducing conditions followed by blotting for the presence of C3 and, after stripping, for Aβ. Arrow , The C3 band at ∼180 kD. ( d ) Preaggregated Aβ 1–42 (50 μM) was incubated in NHS alone, and in the presence of deferoxamine ( Defer. ; 1 mM), glutathione ( Glut. ; 1 mM), dimethylthiourea ( DMTU ; 30 mM), catalase ( CAT ; 2 × 10 4 U/ml), SOD (10 μM), or catalase plus SOD, and the Aβ complexes with C3 activation fragments were then detected as described in Materials and Methods.

    Techniques Used: Binding Assay, Activation Assay, Incubation, Centrifugation, SDS Page, Stripping Membranes

    4) Product Images from "Basis for the phototaxis sign reversal in the green alga Chlamydomonas reinhardtii studied by high-speed observation"

    Article Title: Basis for the phototaxis sign reversal in the green alga Chlamydomonas reinhardtii studied by high-speed observation

    Journal: bioRxiv

    doi: 10.1101/2020.12.06.414052

    Phototaxis assay of the slow-swimming mutants. (A) PP, NP, oda1 , and ida4 cell suspensions put in Petri dishes with or without ROS-modulating reagents (0.2 mM t -BOOH or 75 mM DMTU) were illuminated by green LED (λ=525 nm, 30 μmol photons m −2 s −1 ) from the right (green arrows) for 5 min from the right. Cells showing positive phototaxis are accumulated in the right halves of the dishes (orange boxes with “P”) and those showing negative phototaxis are accumulated in the left halves of the dishes (blue boxes with “N”). (B) Polar histograms depicting the percentage of cells moving in a particular direction relative to light illuminated from the right (green arrows), with or without treatment with ROS-modulating reagents (12 bins of 30°; n = 30 cells per condition).
    Figure Legend Snippet: Phototaxis assay of the slow-swimming mutants. (A) PP, NP, oda1 , and ida4 cell suspensions put in Petri dishes with or without ROS-modulating reagents (0.2 mM t -BOOH or 75 mM DMTU) were illuminated by green LED (λ=525 nm, 30 μmol photons m −2 s −1 ) from the right (green arrows) for 5 min from the right. Cells showing positive phototaxis are accumulated in the right halves of the dishes (orange boxes with “P”) and those showing negative phototaxis are accumulated in the left halves of the dishes (blue boxes with “N”). (B) Polar histograms depicting the percentage of cells moving in a particular direction relative to light illuminated from the right (green arrows), with or without treatment with ROS-modulating reagents (12 bins of 30°; n = 30 cells per condition).

    Techniques Used:

    Eyespot position relative to the cell trajectory during phototaxis. (A) Phototactic turnings of PP and NP cells after treatment with t-BOOH or DMTU. Images of a swimming cell, taken at 150 fps, are superimposed every 0.13 sec. First, Light 1 (weak green light from the left) was illuminated to induce positive (top; swimming to the left) or negative (bottom; swimming to the right) phototaxis. After Light 2 (strong green light from the top) was turned on, the cell changed its swimming direction. The timing of the onset of Light 2 was at the white arrows/arrowhead. The eyespot facing Light 2 is shown with green arrowheads, whereas that facing opposite to Light 2 is shown with magenta arrowheads. The former case is classified as “the light side”) and the latter “the dark side” in (B) . Yellow arrows show the swimming directions. (The cells whose trajectories intersect with the superimposed cells were removed from the image in the process creating the superimpose. See SI Movies S1-4 for the raw data.) (B) The proportion of the dominant cilium and the side where the onset of the ciliary dominance occurred. PP cells showing positive phototaxis (control and + t -BOOH) or negative phototaxis (+DMTU) and NP cells showing negative phototaxis (control and +DMTU) or positive phototaxis (+ t -BOOH) were observed (n=8~28 per condition).
    Figure Legend Snippet: Eyespot position relative to the cell trajectory during phototaxis. (A) Phototactic turnings of PP and NP cells after treatment with t-BOOH or DMTU. Images of a swimming cell, taken at 150 fps, are superimposed every 0.13 sec. First, Light 1 (weak green light from the left) was illuminated to induce positive (top; swimming to the left) or negative (bottom; swimming to the right) phototaxis. After Light 2 (strong green light from the top) was turned on, the cell changed its swimming direction. The timing of the onset of Light 2 was at the white arrows/arrowhead. The eyespot facing Light 2 is shown with green arrowheads, whereas that facing opposite to Light 2 is shown with magenta arrowheads. The former case is classified as “the light side”) and the latter “the dark side” in (B) . Yellow arrows show the swimming directions. (The cells whose trajectories intersect with the superimposed cells were removed from the image in the process creating the superimpose. See SI Movies S1-4 for the raw data.) (B) The proportion of the dominant cilium and the side where the onset of the ciliary dominance occurred. PP cells showing positive phototaxis (control and + t -BOOH) or negative phototaxis (+DMTU) and NP cells showing negative phototaxis (control and +DMTU) or positive phototaxis (+ t -BOOH) were observed (n=8~28 per condition).

    Techniques Used:

    5) Product Images from "Blue-Light Inhibition of Listeria monocytogenes Growth Is Mediated by Reactive Oxygen Species and Is Influenced by σB and the Blue-Light Sensor Lmo0799"

    Article Title: Blue-Light Inhibition of Listeria monocytogenes Growth Is Mediated by Reactive Oxygen Species and Is Influenced by σB and the Blue-Light Sensor Lmo0799

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00685-16

    ROS scavenger DMTU mitigates the inhibitory effect of blue light. EGD-e cells were illuminated with blue light (460 to 470 nm, 1.5 to 2 mW cm −2 ) either on BHI agar (A) or in liquid culture (B) with or without 20 mM DMTU. (A) Overnight cultures
    Figure Legend Snippet: ROS scavenger DMTU mitigates the inhibitory effect of blue light. EGD-e cells were illuminated with blue light (460 to 470 nm, 1.5 to 2 mW cm −2 ) either on BHI agar (A) or in liquid culture (B) with or without 20 mM DMTU. (A) Overnight cultures

    Techniques Used:

    6) Product Images from "Blue-Light Inhibition of Listeria monocytogenes Growth Is Mediated by Reactive Oxygen Species and Is Influenced by σB and the Blue-Light Sensor Lmo0799"

    Article Title: Blue-Light Inhibition of Listeria monocytogenes Growth Is Mediated by Reactive Oxygen Species and Is Influenced by σB and the Blue-Light Sensor Lmo0799

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00685-16

    ROS scavenger DMTU mitigates the inhibitory effect of blue light. EGD-e cells were illuminated with blue light (460 to 470 nm, 1.5 to 2 mW cm −2 ) either on BHI agar (A) or in liquid culture (B) with or without 20 mM DMTU. (A) Overnight cultures
    Figure Legend Snippet: ROS scavenger DMTU mitigates the inhibitory effect of blue light. EGD-e cells were illuminated with blue light (460 to 470 nm, 1.5 to 2 mW cm −2 ) either on BHI agar (A) or in liquid culture (B) with or without 20 mM DMTU. (A) Overnight cultures

    Techniques Used:

    7) Product Images from "The neurotoxic effects of hydrogen peroxide and copper in Retzius nerve cells of the leech Haemopis sanguisuga"

    Article Title: The neurotoxic effects of hydrogen peroxide and copper in Retzius nerve cells of the leech Haemopis sanguisuga

    Journal: Biology Open

    doi: 10.1242/bio.014936

    The effects of the antioxidants on the duration of Retzius nerve cells' action potentials prolonged by H 2 O 2 /Cu(II). The neurotoxic effect of H 2 O 2 /Cu(II) on spontaneous spike electrogenesis of the Retzius neurons was reduced in the presence of the dimethylthiourea (1 mM) and dimethyl sulfoxide (1%), but not the mannitol (5 mM). The measures are expressed as mean±s.d.; * P
    Figure Legend Snippet: The effects of the antioxidants on the duration of Retzius nerve cells' action potentials prolonged by H 2 O 2 /Cu(II). The neurotoxic effect of H 2 O 2 /Cu(II) on spontaneous spike electrogenesis of the Retzius neurons was reduced in the presence of the dimethylthiourea (1 mM) and dimethyl sulfoxide (1%), but not the mannitol (5 mM). The measures are expressed as mean±s.d.; * P

    Techniques Used:

    8) Product Images from "Ethylene is Involved in Brassinosteroids Induced Alternative Respiratory Pathway in Cucumber (Cucumis sativus L.) Seedlings Response to Abiotic Stress"

    Article Title: Ethylene is Involved in Brassinosteroids Induced Alternative Respiratory Pathway in Cucumber (Cucumis sativus L.) Seedlings Response to Abiotic Stress

    Journal: Frontiers in Plant Science

    doi: 10.3389/fpls.2015.00982

    Effects of H 2 O 2 and ethylene inhibitors on BRs-induced AOX capacity in response to environmental stress . (A–D) Plants were pretreated with 100 μM DPI, 5 mM DMTU for 8 h or 1 mM AOA for 12 h, then plants were treated with 1 μM BL 12 h later, plants were challenged with stress conditions for 3 d. Data are the mean ± SD of three biological repeats; the significant difference was analyzed by Student's t -test ( * P
    Figure Legend Snippet: Effects of H 2 O 2 and ethylene inhibitors on BRs-induced AOX capacity in response to environmental stress . (A–D) Plants were pretreated with 100 μM DPI, 5 mM DMTU for 8 h or 1 mM AOA for 12 h, then plants were treated with 1 μM BL 12 h later, plants were challenged with stress conditions for 3 d. Data are the mean ± SD of three biological repeats; the significant difference was analyzed by Student's t -test ( * P

    Techniques Used:

    9) Product Images from "Basis for the phototaxis sign reversal in the green alga Chlamydomonas reinhardtii studied by high-speed observation"

    Article Title: Basis for the phototaxis sign reversal in the green alga Chlamydomonas reinhardtii studied by high-speed observation

    Journal: bioRxiv

    doi: 10.1101/2020.12.06.414052

    Phototaxis assay of the slow-swimming mutants. (A) PP, NP, oda1 , and ida4 cell suspensions put in Petri dishes with or without ROS-modulating reagents (0.2 mM t -BOOH or 75 mM DMTU) were illuminated by green LED (λ=525 nm, 30 μmol photons m −2 s −1 ) from the right (green arrows) for 5 min from the right. Cells showing positive phototaxis are accumulated in the right halves of the dishes (orange boxes with “P”) and those showing negative phototaxis are accumulated in the left halves of the dishes (blue boxes with “N”). (B) Polar histograms depicting the percentage of cells moving in a particular direction relative to light illuminated from the right (green arrows), with or without treatment with ROS-modulating reagents (12 bins of 30°; n = 30 cells per condition).
    Figure Legend Snippet: Phototaxis assay of the slow-swimming mutants. (A) PP, NP, oda1 , and ida4 cell suspensions put in Petri dishes with or without ROS-modulating reagents (0.2 mM t -BOOH or 75 mM DMTU) were illuminated by green LED (λ=525 nm, 30 μmol photons m −2 s −1 ) from the right (green arrows) for 5 min from the right. Cells showing positive phototaxis are accumulated in the right halves of the dishes (orange boxes with “P”) and those showing negative phototaxis are accumulated in the left halves of the dishes (blue boxes with “N”). (B) Polar histograms depicting the percentage of cells moving in a particular direction relative to light illuminated from the right (green arrows), with or without treatment with ROS-modulating reagents (12 bins of 30°; n = 30 cells per condition).

    Techniques Used:

    Eyespot position relative to the cell trajectory during phototaxis. (A) Phototactic turnings of PP and NP cells after treatment with t-BOOH or DMTU. Images of a swimming cell, taken at 150 fps, are superimposed every 0.13 sec. First, Light 1 (weak green light from the left) was illuminated to induce positive (top; swimming to the left) or negative (bottom; swimming to the right) phototaxis. After Light 2 (strong green light from the top) was turned on, the cell changed its swimming direction. The timing of the onset of Light 2 was at the white arrows/arrowhead. The eyespot facing Light 2 is shown with green arrowheads, whereas that facing opposite to Light 2 is shown with magenta arrowheads. The former case is classified as “the light side”) and the latter “the dark side” in (B) . Yellow arrows show the swimming directions. (The cells whose trajectories intersect with the superimposed cells were removed from the image in the process creating the superimpose. See SI Movies S1-4 for the raw data.) (B) The proportion of the dominant cilium and the side where the onset of the ciliary dominance occurred. PP cells showing positive phototaxis (control and + t -BOOH) or negative phototaxis (+DMTU) and NP cells showing negative phototaxis (control and +DMTU) or positive phototaxis (+ t -BOOH) were observed (n=8~28 per condition).
    Figure Legend Snippet: Eyespot position relative to the cell trajectory during phototaxis. (A) Phototactic turnings of PP and NP cells after treatment with t-BOOH or DMTU. Images of a swimming cell, taken at 150 fps, are superimposed every 0.13 sec. First, Light 1 (weak green light from the left) was illuminated to induce positive (top; swimming to the left) or negative (bottom; swimming to the right) phototaxis. After Light 2 (strong green light from the top) was turned on, the cell changed its swimming direction. The timing of the onset of Light 2 was at the white arrows/arrowhead. The eyespot facing Light 2 is shown with green arrowheads, whereas that facing opposite to Light 2 is shown with magenta arrowheads. The former case is classified as “the light side”) and the latter “the dark side” in (B) . Yellow arrows show the swimming directions. (The cells whose trajectories intersect with the superimposed cells were removed from the image in the process creating the superimpose. See SI Movies S1-4 for the raw data.) (B) The proportion of the dominant cilium and the side where the onset of the ciliary dominance occurred. PP cells showing positive phototaxis (control and + t -BOOH) or negative phototaxis (+DMTU) and NP cells showing negative phototaxis (control and +DMTU) or positive phototaxis (+ t -BOOH) were observed (n=8~28 per condition).

    Techniques Used:

    10) Product Images from "The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana"

    Article Title: The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana

    Journal: Journal of Experimental Botany

    doi: 10.1093/jxb/erv328

    Involvement of H 2 O 2 in the BR-induced alternative respiratory pathway. (A, B) Changes of alternative respiration ( V alt ) (A) and NbAOX1 expression (B) in H 2 O 2 scavenger DMTU pre-treated plants as influenced by 0.1 μM BL. N. benthamiana plants were treated with 5mM DMTU for 8h and then treated with 0.1 μM BL for another 24h. Single treatment of BL or DMTU was included as a control. (C, D) Changes in alternative respiration (C) and NbAOX1 expression (D) in NbRBOHB -silenced plants as influenced by 0.1 μM BL. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences ( P
    Figure Legend Snippet: Involvement of H 2 O 2 in the BR-induced alternative respiratory pathway. (A, B) Changes of alternative respiration ( V alt ) (A) and NbAOX1 expression (B) in H 2 O 2 scavenger DMTU pre-treated plants as influenced by 0.1 μM BL. N. benthamiana plants were treated with 5mM DMTU for 8h and then treated with 0.1 μM BL for another 24h. Single treatment of BL or DMTU was included as a control. (C, D) Changes in alternative respiration (C) and NbAOX1 expression (D) in NbRBOHB -silenced plants as influenced by 0.1 μM BL. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences ( P

    Techniques Used: Expressing, Standard Deviation

    Effects of H 2 O 2 on BR-induced NbAOX1 promoter activity. (A, B) Relative GUS (A) or LUC (B) activity under the control of the NbAOX1 promoter in 5mM DMTU pre-treatment plants as influenced by 0.1 μM BL. (C, D) Relative GUS (C) or LUC (D) activity under the control of the NbAOX1 promoter in NbRBOHB -silenced plants as influenced by 0.1 μM BL. N. benthamiana plants were treated with 5mM DMTU for 8h and then treated with 0.1 μM BL for another 24h. Single treatment of BL or DMTU was included as a control. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences ( P
    Figure Legend Snippet: Effects of H 2 O 2 on BR-induced NbAOX1 promoter activity. (A, B) Relative GUS (A) or LUC (B) activity under the control of the NbAOX1 promoter in 5mM DMTU pre-treatment plants as influenced by 0.1 μM BL. (C, D) Relative GUS (C) or LUC (D) activity under the control of the NbAOX1 promoter in NbRBOHB -silenced plants as influenced by 0.1 μM BL. N. benthamiana plants were treated with 5mM DMTU for 8h and then treated with 0.1 μM BL for another 24h. Single treatment of BL or DMTU was included as a control. The CaMV 35S promoter was fused to GUS or LUC as a control for variation in transformation rate. Bars represent mean and standard deviation of values obtained from three biological repeats. Significant differences ( P

    Techniques Used: Activity Assay, Transformation Assay, Standard Deviation

    11) Product Images from "Roles of oxygen radicals and elastase in citric acid-induced airway constriction of guinea-pigs"

    Article Title: Roles of oxygen radicals and elastase in citric acid-induced airway constriction of guinea-pigs

    Journal: British Journal of Pharmacology

    doi: 10.1038/sj.bjp.0702352

    Citric acid-induced alterations in forced expiratory volume in 0.1 s (FEV 0.1 ), expressed as per cent baseline values, in six groups of guinea-pigs. DMTU=dimethylthiourea. Statistical differences ( P
    Figure Legend Snippet: Citric acid-induced alterations in forced expiratory volume in 0.1 s (FEV 0.1 ), expressed as per cent baseline values, in six groups of guinea-pigs. DMTU=dimethylthiourea. Statistical differences ( P

    Techniques Used:

    Citric acid-induced alterations in maximal expiratory flow at 30% vital capacity (V[dot above] max30 ), expressed as per cent baseline values, in six groups of guinea-pigs. DMTU=dimethylthiourea. Statistical differences ( P
    Figure Legend Snippet: Citric acid-induced alterations in maximal expiratory flow at 30% vital capacity (V[dot above] max30 ), expressed as per cent baseline values, in six groups of guinea-pigs. DMTU=dimethylthiourea. Statistical differences ( P

    Techniques Used: Flow Cytometry

    Citric acid-induced alterations in dynamic respiratory compliance (Crs), expressed as per cent baseline values, in six groups of guinea-pigs. DMTU=dimethylthiourea. Statistical differences ( P
    Figure Legend Snippet: Citric acid-induced alterations in dynamic respiratory compliance (Crs), expressed as per cent baseline values, in six groups of guinea-pigs. DMTU=dimethylthiourea. Statistical differences ( P

    Techniques Used:

    12) Product Images from "Effects of Reactive Oxygen Species on in vitro Filtration of Water and Albumin across Glomerular Basement Membrane"

    Article Title: Effects of Reactive Oxygen Species on in vitro Filtration of Water and Albumin across Glomerular Basement Membrane

    Journal: International Journal of Biomedical Science : IJBS

    doi:

    Tracks of change of the volumetric solvent flux in the absence of albumin (J v ) and presence of albumin (J v,alb ) at a concentration of 4g.dL -1 , albumin flux (J s ), and albumin fractional clearance (Θ alb ) with the hydrostatic pressure difference (ΔP) for lisinopril treated rats (LIS, n=8), its negative control group (CTR1, n=7), dimethylthiouria treated rats (DMTU, n=8), and its negative control group (CTR2, n=7). Data are presented as mean ±SD. * p
    Figure Legend Snippet: Tracks of change of the volumetric solvent flux in the absence of albumin (J v ) and presence of albumin (J v,alb ) at a concentration of 4g.dL -1 , albumin flux (J s ), and albumin fractional clearance (Θ alb ) with the hydrostatic pressure difference (ΔP) for lisinopril treated rats (LIS, n=8), its negative control group (CTR1, n=7), dimethylthiouria treated rats (DMTU, n=8), and its negative control group (CTR2, n=7). Data are presented as mean ±SD. * p

    Techniques Used: Concentration Assay, Negative Control

    Body and two kidney weight, urinary protein excretion in 24 hours and systolic blood pressure for lisinopril treated rats (LIS, n = 8), its negative control group (CTR1, n = 7), dimethylthiouria treated rats (DMTU, n = 8), and its negative control group (CTR2, n = 7). Data are presented as mean ± SD. * p
    Figure Legend Snippet: Body and two kidney weight, urinary protein excretion in 24 hours and systolic blood pressure for lisinopril treated rats (LIS, n = 8), its negative control group (CTR1, n = 7), dimethylthiouria treated rats (DMTU, n = 8), and its negative control group (CTR2, n = 7). Data are presented as mean ± SD. * p

    Techniques Used: Negative Control

    Changes in actual hydraulic and local Darcy permeabilities in absence of albumin (L p and K d , respectively) and presence of albumin (L p,alb and K d,alb , respectively) associated with increment changes in the hydrostatic pressure difference (ΔP) for lisinopril treated rats (LIS, n=8), its negative control group (CTR1, n=7), dimethylthiouria treated rats (DMTU, n=8), and its negative control group (CTR2, n=7). Data are presented as mean ± SD. * p
    Figure Legend Snippet: Changes in actual hydraulic and local Darcy permeabilities in absence of albumin (L p and K d , respectively) and presence of albumin (L p,alb and K d,alb , respectively) associated with increment changes in the hydrostatic pressure difference (ΔP) for lisinopril treated rats (LIS, n=8), its negative control group (CTR1, n=7), dimethylthiouria treated rats (DMTU, n=8), and its negative control group (CTR2, n=7). Data are presented as mean ± SD. * p

    Techniques Used: Negative Control

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    Article Snippet: DPI chloride, Evans blue, 4,5-dihydroxy-1,3-benzene-disulfonic acid (Tiron), N ,N ′ -dimethylthiourea (DMTU), were from Sigma (St. Louis, MO, USA).

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    Modification:

    Article Title: Glucose-dependent trans-plasma membrane electron transport and p70S6k phosphorylation in skeletal muscle cells
    Article Snippet: 2.1 Materials C2C12 myoblasts, a mouse muscle cell line, and L6 myoblasts, a rat muscle cell line, were obtained from American Type Culture Collection (Manassas, VA, USA). .. Dulbecco's modified Eagle's medium-low glucose (DMEM), phosphate buffered saline (PBS), penicillin-streptomycin, trypsin-EDTA, phenazine methosulfate (PMS), D -glucose, pyruvate, superoxide dismutase (SOD), 2-deoxy-D -glucose (2DG), dehydroepiandrosterone (DHEA), (2E)-3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO), 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis (acetoxymethyl ester) (BAPTA-AM), diphenyleneiodonium (DPI), N,N′-dimethylthiourea (DMTU), 4-hydroxy-TEMPO (Tempol), and glucose oxidase were purchased from Sigma Aldrich (St. Louis, MO, USA). .. FetalPlex animal serum complex was purchased from Gemini Bio-Products (Woodland, CA, USA).

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  • 93
    Millipore dmtu
    Mitochondrial membrane graphs of MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, <t>trolox</t> and <t>DMTU).</t> ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MDA-MB-231 cells and tiron exposure resulted in an insignificant decrease in membrane depolarization. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.
    Dmtu, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmtu/product/Millipore
    Average 93 stars, based on 1 article reviews
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    dmtu - by Bioz Stars, 2021-04
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    99
    Millipore dimethyl thiourea dmtu
    ROS scavenging capacity of H. inuloides metabolites and semisynthetic derivatives. <t>C=NDGA</t> (superoxide); pyruvate (H 2 O 2 ); <t>DMTU</t> (OH − ); GSH ( 1 O 2 ); ascorbic Ac. acid (HOCl). The results were analyzed by ANOVA. The Dunnett's Multiple Comparison test was used to compare outcomes between experimental and control group. * P
    Dimethyl Thiourea Dmtu, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dimethyl thiourea dmtu/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dimethyl thiourea dmtu - by Bioz Stars, 2021-04
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    97
    Millipore cisplatin dmtu group
    Representative micrographs of renal histopathology in mice at 72 h after <t>cisplatin</t> administration. Tubular necrosis, dilatation, and hyaline cast were observed in the cisplatin group, and the <t>DMTU</t> treatment suppressed the severity of the tissue damage. Bar = 50 μm
    Cisplatin Dmtu Group, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mitochondrial membrane graphs of MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU). ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MDA-MB-231 cells and tiron exposure resulted in an insignificant decrease in membrane depolarization. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Mitochondrial membrane graphs of MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU). ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MDA-MB-231 cells and tiron exposure resulted in an insignificant decrease in membrane depolarization. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Multiple Displacement Amplification, Cell Culture

    Mitochondrial membrane graphs of MCF-7 cells treated with ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU). ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MCF-7 cells and tiron countered that effect significantly. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Mitochondrial membrane graphs of MCF-7 cells treated with ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU). ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MCF-7 cells and tiron countered that effect significantly. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Cell Culture

    Cell cycle progression graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU) for 24 h. ESE-one exposure resulted in a G 2 /M block in both MCF-7 and MDA-MB-231 cells. Tiron and DMTU exposure significantly decreased the number of cells blocked in sub-G 1 phase in MDA-MB-231 cells and tiron as well as trolox exposure significantly decreased the number of cells blocked in G 2 /M phase in both MCF-7 and MDA-MB-231 cells. ( a ) MCF-7 cells cultured in complete growth medium, ( b ) vehicle-treated MCF-7 cells, ( c ) MCF-7 cells exposed to ESE-one only, ( d ) MDA-MB-231 cells cultured in complete growth medium, ( e ) vehicle-treated MDA-MB-231 cells, ( f ) MDA-MB-231 cells exposed to ESE-one, ( g ) MCF-7 cells exposed to tiron only, ( h ) MCF-7 cells exposed to trolox only, ( i ) MCF-7 cells exposed to DMTU only, ( j ) MCF-7 cells coexposed to tiron and ESE-one, ( k ) MCF-7 cells coexposed to trolox and ESE-one, ( l ) MCF-7 cells coexposed to DMTU and ESE-one, ( m ) MDA-MB-231 cells coexposed to tiron only, ( n ) MDA-MB-231 cells exposed to trolox only, ( o ) MDA-MB-231 cells exposed to DMTU only, ( p ) MDA-MB-231 cells coexposed to tiron and ESE-one, ( q ) MDA-MB-231 cells coexposed to trolox and ESE-one, ( r ) MDA-MB-231 cells coexposed to DMTU and ESE-one.

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Cell cycle progression graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU) for 24 h. ESE-one exposure resulted in a G 2 /M block in both MCF-7 and MDA-MB-231 cells. Tiron and DMTU exposure significantly decreased the number of cells blocked in sub-G 1 phase in MDA-MB-231 cells and tiron as well as trolox exposure significantly decreased the number of cells blocked in G 2 /M phase in both MCF-7 and MDA-MB-231 cells. ( a ) MCF-7 cells cultured in complete growth medium, ( b ) vehicle-treated MCF-7 cells, ( c ) MCF-7 cells exposed to ESE-one only, ( d ) MDA-MB-231 cells cultured in complete growth medium, ( e ) vehicle-treated MDA-MB-231 cells, ( f ) MDA-MB-231 cells exposed to ESE-one, ( g ) MCF-7 cells exposed to tiron only, ( h ) MCF-7 cells exposed to trolox only, ( i ) MCF-7 cells exposed to DMTU only, ( j ) MCF-7 cells coexposed to tiron and ESE-one, ( k ) MCF-7 cells coexposed to trolox and ESE-one, ( l ) MCF-7 cells coexposed to DMTU and ESE-one, ( m ) MDA-MB-231 cells coexposed to tiron only, ( n ) MDA-MB-231 cells exposed to trolox only, ( o ) MDA-MB-231 cells exposed to DMTU only, ( p ) MDA-MB-231 cells coexposed to tiron and ESE-one, ( q ) MDA-MB-231 cells coexposed to trolox and ESE-one, ( r ) MDA-MB-231 cells coexposed to DMTU and ESE-one.

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Multiple Displacement Amplification, Blocking Assay, Cell Culture

    Cell cycle progression graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of various scavengers of reactive oxygen species (tiron, trolox and DMTU) for 48 h. ESE-one exposure resulted in a significant increase in the percentage of cells occupying the sub-G 1 phase in both MCF-7 and MDA-MB-231 cells. Tiron, trolox and DMTU exposure significantly decreased the number of cells occupying the sub-G 1 phase in MCF-7 cells and tiron as well as DMTU exposure significantly decreased the number of cells present in the sub-G 1 phase in MDA-MB-231 cells. Tiron, trolox and DMTU significantly decreased the number of cells in the G 2 /M phase in MDA-MB-231 cells and only tiron had a significant effect in MCF-7 cells. ( a ) MCF-7 cells cultured in complete growth medium, ( b ) vehicle-treated MCF-7 cells, ( c ) MCF-7 cells exposed to ESE-one only, ( d ) MDA-MB-231 cells cultured in complete growth medium, ( e ) vehicle-treated MDA-MB-231 cells, ( f ) MDA-MB-231 cells exposed to ESE-one, ( g ) MCF-7 cells exposed to tiron only, ( h ) MCF-7 cells exposed to trolox only, ( i ) MCF-7 cells exposed to DMTU only, ( j ) MCF-7 cells coexposed to tiron and ESE-one, ( k ) MCF-7 cells coexposed to trolox and ESE-one, ( l ) MCF-7 cells coexposed to DMTU and ESE-one, ( m ) MDA-MB-231 cells exposed to tiron only, ( n ) MDA-MB-231 cells exposed to trolox only, ( o ) MDA-MB-231 cells exposed to DMTU only, ( p ) MDA-MB-231 cells coexposed to tiron and ESE-one, ( q ) MDA-MB-231 cells coexposed to trolox and ESE-one, ( r ) MDA-MB-231 cells coexposed to DMTU and ESE-one.

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Cell cycle progression graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of various scavengers of reactive oxygen species (tiron, trolox and DMTU) for 48 h. ESE-one exposure resulted in a significant increase in the percentage of cells occupying the sub-G 1 phase in both MCF-7 and MDA-MB-231 cells. Tiron, trolox and DMTU exposure significantly decreased the number of cells occupying the sub-G 1 phase in MCF-7 cells and tiron as well as DMTU exposure significantly decreased the number of cells present in the sub-G 1 phase in MDA-MB-231 cells. Tiron, trolox and DMTU significantly decreased the number of cells in the G 2 /M phase in MDA-MB-231 cells and only tiron had a significant effect in MCF-7 cells. ( a ) MCF-7 cells cultured in complete growth medium, ( b ) vehicle-treated MCF-7 cells, ( c ) MCF-7 cells exposed to ESE-one only, ( d ) MDA-MB-231 cells cultured in complete growth medium, ( e ) vehicle-treated MDA-MB-231 cells, ( f ) MDA-MB-231 cells exposed to ESE-one, ( g ) MCF-7 cells exposed to tiron only, ( h ) MCF-7 cells exposed to trolox only, ( i ) MCF-7 cells exposed to DMTU only, ( j ) MCF-7 cells coexposed to tiron and ESE-one, ( k ) MCF-7 cells coexposed to trolox and ESE-one, ( l ) MCF-7 cells coexposed to DMTU and ESE-one, ( m ) MDA-MB-231 cells exposed to tiron only, ( n ) MDA-MB-231 cells exposed to trolox only, ( o ) MDA-MB-231 cells exposed to DMTU only, ( p ) MDA-MB-231 cells coexposed to tiron and ESE-one, ( q ) MDA-MB-231 cells coexposed to trolox and ESE-one, ( r ) MDA-MB-231 cells coexposed to DMTU and ESE-one.

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Multiple Displacement Amplification, Cell Culture

    Catalase activity graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of scavengers of reactive oxygen species (tiron, trolox and DMTU). Tiron and DMTU coexposure with ESE-one increased the catalase protein concentration significantly in MCF-7 cells and in MDA-MB-231 cells, tiron and trolox induced a significant increase in catalase protein concentration. ( a ) MCF-7, ( b ) MDA-MB-231. An asterisk (*) indicates p -value ( p

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Catalase activity graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of scavengers of reactive oxygen species (tiron, trolox and DMTU). Tiron and DMTU coexposure with ESE-one increased the catalase protein concentration significantly in MCF-7 cells and in MDA-MB-231 cells, tiron and trolox induced a significant increase in catalase protein concentration. ( a ) MCF-7, ( b ) MDA-MB-231. An asterisk (*) indicates p -value ( p

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Activity Assay, Multiple Displacement Amplification, Protein Concentration

    SOD inhibition graphs of ( a ) MCF-7 and ( b ) MDA-MB-231 cells exposed to ESE-one in the presence or absence of scavengers of reactive oxygen species (tiron, trolox and DMTU). Tiron, trolox and DMTU coexposure with ESE-one resulted in an increased SOD inhibition percentage compared to ESE-one only exposure in MCF-7 cells suggesting that increased generation of reactive oxygen species due to ESE-one exposure is not required for the inhibition of SOD. In MDA-MB-231, DMTU demonstrated a decrease in SOD inhibition percentage suggesting that hydrogen peroxide affects the superoxide anion activity. ( a ) MCF-7, ( b ) MDA-MB-231. An asterisk (*) indicates p -value ( p

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: SOD inhibition graphs of ( a ) MCF-7 and ( b ) MDA-MB-231 cells exposed to ESE-one in the presence or absence of scavengers of reactive oxygen species (tiron, trolox and DMTU). Tiron, trolox and DMTU coexposure with ESE-one resulted in an increased SOD inhibition percentage compared to ESE-one only exposure in MCF-7 cells suggesting that increased generation of reactive oxygen species due to ESE-one exposure is not required for the inhibition of SOD. In MDA-MB-231, DMTU demonstrated a decrease in SOD inhibition percentage suggesting that hydrogen peroxide affects the superoxide anion activity. ( a ) MCF-7, ( b ) MDA-MB-231. An asterisk (*) indicates p -value ( p

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Inhibition, Multiple Displacement Amplification, Activity Assay

    ROS scavenging capacity of H. inuloides metabolites and semisynthetic derivatives. C=NDGA (superoxide); pyruvate (H 2 O 2 ); DMTU (OH − ); GSH ( 1 O 2 ); ascorbic Ac. acid (HOCl). The results were analyzed by ANOVA. The Dunnett's Multiple Comparison test was used to compare outcomes between experimental and control group. * P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Antioxidant Capacity of “Mexican Arnica” Heterotheca inuloides Cass Natural Products and Some Derivatives: Their Anti-Inflammatory Evaluation and Effect on C. elegans Life Span

    doi: 10.1155/2015/843237

    Figure Lengend Snippet: ROS scavenging capacity of H. inuloides metabolites and semisynthetic derivatives. C=NDGA (superoxide); pyruvate (H 2 O 2 ); DMTU (OH − ); GSH ( 1 O 2 ); ascorbic Ac. acid (HOCl). The results were analyzed by ANOVA. The Dunnett's Multiple Comparison test was used to compare outcomes between experimental and control group. * P

    Article Snippet: Sodium pyruvate, dimethyl thiourea (DMTU), nordihydroguaiaretic acid (NDGA), ascorbic acid, histidine, xylenol orange (FOX), 2,2-diphenyl-1-picrylhydrazyl (DPPH), dimethylsulfoxide (DMSO), N,N-dimethyl-4-nitrosoaniline (DMNA), catalase, xanthine, xanthine oxidase, nitroblue tetrazolium (NBT), dL-penicillamine 2-thiobarbituric acid (TBA), α -tocopherol, Folin and Ciocalteu's phenol reagent, 3,5-di-tert-4-butylhydroxytoluene (BHT), and 5-fluoro-2′-deoxyuridine were purchased from Sigma-Aldrich (Toluca, Mexico, or Sigma, St. Louis, MO).

    Techniques:

    Representative micrographs of renal histopathology in mice at 72 h after cisplatin administration. Tubular necrosis, dilatation, and hyaline cast were observed in the cisplatin group, and the DMTU treatment suppressed the severity of the tissue damage. Bar = 50 μm

    Journal: EJNMMI Research

    Article Title: Imaging of reactive oxygen species using [3H]hydromethidine in mice with cisplatin-induced nephrotoxicity

    doi: 10.1186/s13550-015-0116-0

    Figure Lengend Snippet: Representative micrographs of renal histopathology in mice at 72 h after cisplatin administration. Tubular necrosis, dilatation, and hyaline cast were observed in the cisplatin group, and the DMTU treatment suppressed the severity of the tissue damage. Bar = 50 μm

    Article Snippet: Mice of the cisplatin + DMTU group were intraperitoneally administered with DMTU (100-mg/kg body weight, Sigma-Aldrich, St. Louis, MO, USA) dissolved in saline at 10 mg/mL, 30 min prior to the cisplatin administration (30 mg/kg).

    Techniques: Histopathology, Mouse Assay