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Dade Behring diff-quik ® fixative/stain set
Diff Quik ® Fixative/Stain Set, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quik+fixative/diff+quik+stain+set/pmc12139374-64-20-24
Average 90 stars, based on 1 article reviews
diff-quik ® fixative/stain set - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Incubation:

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum-infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Novel circular RNA NF1 acts as a molecular sponge, promoting gastric cancer by absorbing miR-16
Article Snippet: Then, the plates were washed twice with PBS and stained with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes each, in sequence at room temperature.

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum -infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Synthetic circular multi-miR sponge simultaneously inhibits miR-21 and miR-93 in esophageal carcinoma.
Article Snippet: After 10 days of incubation, the plates were washed twice with PBS and dyed with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes successively at room temperature.

Article Title: Krüppel-like Factor 5 Promotes Sonic Hedgehog Signaling and Neoplasia in Barrett's Esophagus and Esophageal Adenocarcinoma
Article Snippet: Cells were sequentially stained with Diff-Quik Fixative, Diff-Quik Solution I, and Diff-Quik Solution II (Dade Behring Inc., Newark, DE) for 10 minutes each at room temperature.

Article Title: TIMP-2 promotes cell spreading and adhesion via upregulation of Rap1 signaling.
Article Snippet: We previously demonstrated that TIMP-2 treatment of human microvascular endothelial cells (hMVECs) activates Rap1 via the pathway of paxillin-Crk-C3G.. Here, we show that TIMP-2 overexpression in hMVECs by adenoviral infection enhances Rap1 expression, leading to further increase in Rap1-GTP.. TIMP-2 expression, previously reported to inhibit cell migration, also leads to cell spreading accompanied with increased cell adhesion.

Article Title: Method of reducing serum glucose and triglyceride levels and for inhibiting angiogenesis using substitute indolealkanoic acids
Article Snippet: At the completion of the study, the media is aspirated and Diff-Quik fixative (Dade-Behring) is added to each well as per manufacturer's instructions to preserve the specimens which are stored sealed and refrigerated at 4° C. The antigiogenic effect of the compound of Example 1 is shown in Table 3 below.

Article Title: Nm23-H1 Suppresses Metastasis by Inhibiting Expression of the Lysophosphatidic Acid Receptor EDG2
Article Snippet: Cells attached to the membrane were fixed with Diff-Quik Fixative (Dade-Behring) and stained with Diff-Quik solutions I and II.

Diff-Quik:

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum-infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Novel circular RNA NF1 acts as a molecular sponge, promoting gastric cancer by absorbing miR-16
Article Snippet: Then, the plates were washed twice with PBS and stained with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes each, in sequence at room temperature.

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum -infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Synthetic circular multi-miR sponge simultaneously inhibits miR-21 and miR-93 in esophageal carcinoma.
Article Snippet: After 10 days of incubation, the plates were washed twice with PBS and dyed with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes successively at room temperature.

Article Title: Krüppel-like Factor 5 Promotes Sonic Hedgehog Signaling and Neoplasia in Barrett's Esophagus and Esophageal Adenocarcinoma
Article Snippet: Cells were sequentially stained with Diff-Quik Fixative, Diff-Quik Solution I, and Diff-Quik Solution II (Dade Behring Inc., Newark, DE) for 10 minutes each at room temperature.

Article Title: TIMP-2 promotes cell spreading and adhesion via upregulation of Rap1 signaling.
Article Snippet: We previously demonstrated that TIMP-2 treatment of human microvascular endothelial cells (hMVECs) activates Rap1 via the pathway of paxillin-Crk-C3G.. Here, we show that TIMP-2 overexpression in hMVECs by adenoviral infection enhances Rap1 expression, leading to further increase in Rap1-GTP.. TIMP-2 expression, previously reported to inhibit cell migration, also leads to cell spreading accompanied with increased cell adhesion.

Article Title: Method of reducing serum glucose and triglyceride levels and for inhibiting angiogenesis using substitute indolealkanoic acids
Article Snippet: At the completion of the study, the media is aspirated and Diff-Quik fixative (Dade-Behring) is added to each well as per manufacturer's instructions to preserve the specimens which are stored sealed and refrigerated at 4° C. The antigiogenic effect of the compound of Example 1 is shown in Table 3 below.

Article Title: Nm23-H1 Suppresses Metastasis by Inhibiting Expression of the Lysophosphatidic Acid Receptor EDG2
Article Snippet: Cells attached to the membrane were fixed with Diff-Quik Fixative (Dade-Behring) and stained with Diff-Quik solutions I and II.

Staining:

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum-infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Novel circular RNA NF1 acts as a molecular sponge, promoting gastric cancer by absorbing miR-16
Article Snippet: Then, the plates were washed twice with PBS and stained with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes each, in sequence at room temperature.

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum -infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Synthetic circular multi-miR sponge simultaneously inhibits miR-21 and miR-93 in esophageal carcinoma.
Article Snippet: After 10 days of incubation, the plates were washed twice with PBS and dyed with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes successively at room temperature.

Article Title: Krüppel-like Factor 5 Promotes Sonic Hedgehog Signaling and Neoplasia in Barrett's Esophagus and Esophageal Adenocarcinoma
Article Snippet: Cells were sequentially stained with Diff-Quik Fixative, Diff-Quik Solution I, and Diff-Quik Solution II (Dade Behring Inc., Newark, DE) for 10 minutes each at room temperature.

Article Title: TIMP-2 promotes cell spreading and adhesion via upregulation of Rap1 signaling.
Article Snippet: We previously demonstrated that TIMP-2 treatment of human microvascular endothelial cells (hMVECs) activates Rap1 via the pathway of paxillin-Crk-C3G.. Here, we show that TIMP-2 overexpression in hMVECs by adenoviral infection enhances Rap1 expression, leading to further increase in Rap1-GTP.. TIMP-2 expression, previously reported to inhibit cell migration, also leads to cell spreading accompanied with increased cell adhesion.

Article Title: Method of reducing serum glucose and triglyceride levels and for inhibiting angiogenesis using substitute indolealkanoic acids
Article Snippet: At the completion of the study, the media is aspirated and Diff-Quik fixative (Dade-Behring) is added to each well as per manufacturer's instructions to preserve the specimens which are stored sealed and refrigerated at 4° C. The antigiogenic effect of the compound of Example 1 is shown in Table 3 below.

Article Title: Nm23-H1 Suppresses Metastasis by Inhibiting Expression of the Lysophosphatidic Acid Receptor EDG2
Article Snippet: Cells attached to the membrane were fixed with Diff-Quik Fixative (Dade-Behring) and stained with Diff-Quik solutions I and II.

Sequencing:

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum-infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Novel circular RNA NF1 acts as a molecular sponge, promoting gastric cancer by absorbing miR-16
Article Snippet: Then, the plates were washed twice with PBS and stained with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes each, in sequence at room temperature.

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum -infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Synthetic circular multi-miR sponge simultaneously inhibits miR-21 and miR-93 in esophageal carcinoma.
Article Snippet: After 10 days of incubation, the plates were washed twice with PBS and dyed with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes successively at room temperature.

Article Title: Krüppel-like Factor 5 Promotes Sonic Hedgehog Signaling and Neoplasia in Barrett's Esophagus and Esophageal Adenocarcinoma
Article Snippet: Cells were sequentially stained with Diff-Quik Fixative, Diff-Quik Solution I, and Diff-Quik Solution II (Dade Behring Inc., Newark, DE) for 10 minutes each at room temperature.

Article Title: TIMP-2 promotes cell spreading and adhesion via upregulation of Rap1 signaling.
Article Snippet: We previously demonstrated that TIMP-2 treatment of human microvascular endothelial cells (hMVECs) activates Rap1 via the pathway of paxillin-Crk-C3G.. Here, we show that TIMP-2 overexpression in hMVECs by adenoviral infection enhances Rap1 expression, leading to further increase in Rap1-GTP.. TIMP-2 expression, previously reported to inhibit cell migration, also leads to cell spreading accompanied with increased cell adhesion.

Article Title: Method of reducing serum glucose and triglyceride levels and for inhibiting angiogenesis using substitute indolealkanoic acids
Article Snippet: At the completion of the study, the media is aspirated and Diff-Quik fixative (Dade-Behring) is added to each well as per manufacturer's instructions to preserve the specimens which are stored sealed and refrigerated at 4° C. The antigiogenic effect of the compound of Example 1 is shown in Table 3 below.

Article Title: Nm23-H1 Suppresses Metastasis by Inhibiting Expression of the Lysophosphatidic Acid Receptor EDG2
Article Snippet: Cells attached to the membrane were fixed with Diff-Quik Fixative (Dade-Behring) and stained with Diff-Quik solutions I and II.

Infection:

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum-infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Novel circular RNA NF1 acts as a molecular sponge, promoting gastric cancer by absorbing miR-16
Article Snippet: Then, the plates were washed twice with PBS and stained with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes each, in sequence at room temperature.

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum -infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Synthetic circular multi-miR sponge simultaneously inhibits miR-21 and miR-93 in esophageal carcinoma.
Article Snippet: After 10 days of incubation, the plates were washed twice with PBS and dyed with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes successively at room temperature.

Article Title: Krüppel-like Factor 5 Promotes Sonic Hedgehog Signaling and Neoplasia in Barrett's Esophagus and Esophageal Adenocarcinoma
Article Snippet: Cells were sequentially stained with Diff-Quik Fixative, Diff-Quik Solution I, and Diff-Quik Solution II (Dade Behring Inc., Newark, DE) for 10 minutes each at room temperature.

Article Title: TIMP-2 promotes cell spreading and adhesion via upregulation of Rap1 signaling.
Article Snippet: We previously demonstrated that TIMP-2 treatment of human microvascular endothelial cells (hMVECs) activates Rap1 via the pathway of paxillin-Crk-C3G.. Here, we show that TIMP-2 overexpression in hMVECs by adenoviral infection enhances Rap1 expression, leading to further increase in Rap1-GTP.. TIMP-2 expression, previously reported to inhibit cell migration, also leads to cell spreading accompanied with increased cell adhesion.

Article Title: Method of reducing serum glucose and triglyceride levels and for inhibiting angiogenesis using substitute indolealkanoic acids
Article Snippet: At the completion of the study, the media is aspirated and Diff-Quik fixative (Dade-Behring) is added to each well as per manufacturer's instructions to preserve the specimens which are stored sealed and refrigerated at 4° C. The antigiogenic effect of the compound of Example 1 is shown in Table 3 below.

Article Title: Nm23-H1 Suppresses Metastasis by Inhibiting Expression of the Lysophosphatidic Acid Receptor EDG2
Article Snippet: Cells attached to the membrane were fixed with Diff-Quik Fixative (Dade-Behring) and stained with Diff-Quik solutions I and II.

Membrane:

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum-infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Novel circular RNA NF1 acts as a molecular sponge, promoting gastric cancer by absorbing miR-16
Article Snippet: Then, the plates were washed twice with PBS and stained with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes each, in sequence at room temperature.

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum -infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Synthetic circular multi-miR sponge simultaneously inhibits miR-21 and miR-93 in esophageal carcinoma.
Article Snippet: After 10 days of incubation, the plates were washed twice with PBS and dyed with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes successively at room temperature.

Article Title: Krüppel-like Factor 5 Promotes Sonic Hedgehog Signaling and Neoplasia in Barrett's Esophagus and Esophageal Adenocarcinoma
Article Snippet: Cells were sequentially stained with Diff-Quik Fixative, Diff-Quik Solution I, and Diff-Quik Solution II (Dade Behring Inc., Newark, DE) for 10 minutes each at room temperature.

Article Title: TIMP-2 promotes cell spreading and adhesion via upregulation of Rap1 signaling.
Article Snippet: We previously demonstrated that TIMP-2 treatment of human microvascular endothelial cells (hMVECs) activates Rap1 via the pathway of paxillin-Crk-C3G.. Here, we show that TIMP-2 overexpression in hMVECs by adenoviral infection enhances Rap1 expression, leading to further increase in Rap1-GTP.. TIMP-2 expression, previously reported to inhibit cell migration, also leads to cell spreading accompanied with increased cell adhesion.

Article Title: Method of reducing serum glucose and triglyceride levels and for inhibiting angiogenesis using substitute indolealkanoic acids
Article Snippet: At the completion of the study, the media is aspirated and Diff-Quik fixative (Dade-Behring) is added to each well as per manufacturer's instructions to preserve the specimens which are stored sealed and refrigerated at 4° C. The antigiogenic effect of the compound of Example 1 is shown in Table 3 below.

Article Title: Nm23-H1 Suppresses Metastasis by Inhibiting Expression of the Lysophosphatidic Acid Receptor EDG2
Article Snippet: Cells attached to the membrane were fixed with Diff-Quik Fixative (Dade-Behring) and stained with Diff-Quik solutions I and II.

Concentration Assay:

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum-infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Novel circular RNA NF1 acts as a molecular sponge, promoting gastric cancer by absorbing miR-16
Article Snippet: Then, the plates were washed twice with PBS and stained with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes each, in sequence at room temperature.

Article Title: The Anaplasma phagocytophilum PleC Histidine Kinase and PleD Diguanylate Cyclase Two-Component System and Role of Cyclic Di-GMP in Host Cell Infection
Article Snippet: At 2 days postinfection (p.i.), A. phagocytophilum -infected HL-60 cells (60% infected cells) were cytocentrifuged onto a glass slide and fixed for 15 s in Diff-Quik Fixative (Dade Behring Inc., Newark, DE) at room temperature.

Article Title: Synthetic circular multi-miR sponge simultaneously inhibits miR-21 and miR-93 in esophageal carcinoma.
Article Snippet: After 10 days of incubation, the plates were washed twice with PBS and dyed with Diff-Quik Fixative, Diff-Quik Solution I and Diff-Quik Solution II (Dade Behring Inc, Newark, DE) for 10 minutes successively at room temperature.

Article Title: Krüppel-like Factor 5 Promotes Sonic Hedgehog Signaling and Neoplasia in Barrett's Esophagus and Esophageal Adenocarcinoma
Article Snippet: Cells were sequentially stained with Diff-Quik Fixative, Diff-Quik Solution I, and Diff-Quik Solution II (Dade Behring Inc., Newark, DE) for 10 minutes each at room temperature.

Article Title: TIMP-2 promotes cell spreading and adhesion via upregulation of Rap1 signaling.
Article Snippet: We previously demonstrated that TIMP-2 treatment of human microvascular endothelial cells (hMVECs) activates Rap1 via the pathway of paxillin-Crk-C3G.. Here, we show that TIMP-2 overexpression in hMVECs by adenoviral infection enhances Rap1 expression, leading to further increase in Rap1-GTP.. TIMP-2 expression, previously reported to inhibit cell migration, also leads to cell spreading accompanied with increased cell adhesion.

Article Title: Method of reducing serum glucose and triglyceride levels and for inhibiting angiogenesis using substitute indolealkanoic acids
Article Snippet: At the completion of the study, the media is aspirated and Diff-Quik fixative (Dade-Behring) is added to each well as per manufacturer's instructions to preserve the specimens which are stored sealed and refrigerated at 4° C. The antigiogenic effect of the compound of Example 1 is shown in Table 3 below.

Article Title: Nm23-H1 Suppresses Metastasis by Inhibiting Expression of the Lysophosphatidic Acid Receptor EDG2
Article Snippet: Cells attached to the membrane were fixed with Diff-Quik Fixative (Dade-Behring) and stained with Diff-Quik solutions I and II.



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90
Dade Behring diff-quik® fixative and stain set
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Diff Quik® Fixative And Stain Set, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quik+fixative/diff+quik+stain+set/med_rxiv__2020__08__23__20177162-57-24-26
Average 90 stars, based on 1 article reviews
diff-quik® fixative and stain set - by Bioz Stars, 2026-09
90/100 stars
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90
Dade Behring diff-quik fixative
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Diff Quik Fixative, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quik+fixative/diff+quik+stain+set/pm31217510-93-15-24
Average 90 stars, based on 1 article reviews
diff-quik fixative - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Journal: Frontiers in Immunology

Article Title: Neutrophil Extracellular Traps Activate Proinflammatory Functions of Human Neutrophils

doi: 10.3389/fimmu.2021.636954

Figure Lengend Snippet: Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Article Snippet: The preparations contained >99% granulocytes, of which >96% were neutrophils and 1%–4% were eosinophils, as determined by Giemsa staining (Diff Quik ® Fix, Medion Diagnostics, Berlin, Germany) of cytocentrifuged (Shandon) samples ( ).

Techniques: Activation Assay, Flow Cytometry, Staining, Fluorescence, Microscopy