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Fisher Scientific diff quick staining
Diff Quick Staining, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick/diff+quick/pm42304197-144-11-13
Average 86 stars, based on 1 article reviews
diff quick staining - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Staining:

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Article Title: Subchronic intranasal lipopolysaccharide exposure induces pulmonary autoimmunity and glomerulonephritis in NZBWF1 mice.
Article Snippet: In preparation for differential cell counts, BALF cells were immobilized on glass slides using centrifugal forces (Shandon Cytospin 3) and spun down at 40 x g for 10 min. Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice.
Article Snippet: Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Pulmonary toxicity of sodium dichloroisocyanurate after intratracheal instillation in sprague-dawley rats.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, Swedesboro, NJ, USA).

Article Title: ROBO1 protein expression is independently associated with biochemical recurrence in prostate cancer patients who underwent radical prostatectomy in Asian patients
Article Snippet: After 24 h, the cells that penetrated to the underside surface of the inserts were fixed and stained with Diff-Quick (Fisher Scientific, Pittsburgh, PA, USA) and counted and compared to native prostate cancer cells and knockdown cells under light microscopy.

Article Title: Topical Imiquimod Therapy for Localized Solar Dermatitis in a Dog.
Article Snippet: Chronic sun exposure in dogs can result in clinical changes in the skin referred to as solar or actinic dermatitis.. A 5-year-old, neutered male, American Bulldog presented with localized unilateral erythematous macules, plaques, alopecia, comedones and hemorrhagic bullae involving the non-pigmented skin on left lateral ventral flank area.. Thepresence of hyperplastic epidermiswith keratinocyte dysplasia, superficial dermal elastosis, and multiple follicular cysts with occasional rupture (furunculosis) on histology, together with history and characteristic skin lesions was consistent solar dermatitis/actinic keratosis.

Article Title: Comparative effects of human-equivalent low, moderate, and high dose oral prednisone intake on autoimmunity and glucocorticoid-related toxicity in a murine model of environmental-triggered lupus
Article Snippet: Cytological slides were prepared by centrifuging BALF at 400 x g for 10 min using the Shandon Cytospin 3 (Shandon Scientific, PA) and stained with Diff-Quick (Fisher Scientific).

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells
Article Snippet: The slides were stained with Diff‐Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Diff-Quik:

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Article Title: Subchronic intranasal lipopolysaccharide exposure induces pulmonary autoimmunity and glomerulonephritis in NZBWF1 mice.
Article Snippet: In preparation for differential cell counts, BALF cells were immobilized on glass slides using centrifugal forces (Shandon Cytospin 3) and spun down at 40 x g for 10 min. Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice.
Article Snippet: Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Pulmonary toxicity of sodium dichloroisocyanurate after intratracheal instillation in sprague-dawley rats.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, Swedesboro, NJ, USA).

Article Title: ROBO1 protein expression is independently associated with biochemical recurrence in prostate cancer patients who underwent radical prostatectomy in Asian patients
Article Snippet: After 24 h, the cells that penetrated to the underside surface of the inserts were fixed and stained with Diff-Quick (Fisher Scientific, Pittsburgh, PA, USA) and counted and compared to native prostate cancer cells and knockdown cells under light microscopy.

Article Title: Topical Imiquimod Therapy for Localized Solar Dermatitis in a Dog.
Article Snippet: Chronic sun exposure in dogs can result in clinical changes in the skin referred to as solar or actinic dermatitis.. A 5-year-old, neutered male, American Bulldog presented with localized unilateral erythematous macules, plaques, alopecia, comedones and hemorrhagic bullae involving the non-pigmented skin on left lateral ventral flank area.. Thepresence of hyperplastic epidermiswith keratinocyte dysplasia, superficial dermal elastosis, and multiple follicular cysts with occasional rupture (furunculosis) on histology, together with history and characteristic skin lesions was consistent solar dermatitis/actinic keratosis.

Article Title: Comparative effects of human-equivalent low, moderate, and high dose oral prednisone intake on autoimmunity and glucocorticoid-related toxicity in a murine model of environmental-triggered lupus
Article Snippet: Cytological slides were prepared by centrifuging BALF at 400 x g for 10 min using the Shandon Cytospin 3 (Shandon Scientific, PA) and stained with Diff-Quick (Fisher Scientific).

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells
Article Snippet: The slides were stained with Diff‐Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Microscopy:

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Article Title: Subchronic intranasal lipopolysaccharide exposure induces pulmonary autoimmunity and glomerulonephritis in NZBWF1 mice.
Article Snippet: In preparation for differential cell counts, BALF cells were immobilized on glass slides using centrifugal forces (Shandon Cytospin 3) and spun down at 40 x g for 10 min. Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice.
Article Snippet: Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Pulmonary toxicity of sodium dichloroisocyanurate after intratracheal instillation in sprague-dawley rats.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, Swedesboro, NJ, USA).

Article Title: ROBO1 protein expression is independently associated with biochemical recurrence in prostate cancer patients who underwent radical prostatectomy in Asian patients
Article Snippet: After 24 h, the cells that penetrated to the underside surface of the inserts were fixed and stained with Diff-Quick (Fisher Scientific, Pittsburgh, PA, USA) and counted and compared to native prostate cancer cells and knockdown cells under light microscopy.

Article Title: Topical Imiquimod Therapy for Localized Solar Dermatitis in a Dog.
Article Snippet: Chronic sun exposure in dogs can result in clinical changes in the skin referred to as solar or actinic dermatitis.. A 5-year-old, neutered male, American Bulldog presented with localized unilateral erythematous macules, plaques, alopecia, comedones and hemorrhagic bullae involving the non-pigmented skin on left lateral ventral flank area.. Thepresence of hyperplastic epidermiswith keratinocyte dysplasia, superficial dermal elastosis, and multiple follicular cysts with occasional rupture (furunculosis) on histology, together with history and characteristic skin lesions was consistent solar dermatitis/actinic keratosis.

Article Title: Comparative effects of human-equivalent low, moderate, and high dose oral prednisone intake on autoimmunity and glucocorticoid-related toxicity in a murine model of environmental-triggered lupus
Article Snippet: Cytological slides were prepared by centrifuging BALF at 400 x g for 10 min using the Shandon Cytospin 3 (Shandon Scientific, PA) and stained with Diff-Quick (Fisher Scientific).

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells
Article Snippet: The slides were stained with Diff‐Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Infection:

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Article Title: Subchronic intranasal lipopolysaccharide exposure induces pulmonary autoimmunity and glomerulonephritis in NZBWF1 mice.
Article Snippet: In preparation for differential cell counts, BALF cells were immobilized on glass slides using centrifugal forces (Shandon Cytospin 3) and spun down at 40 x g for 10 min. Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice.
Article Snippet: Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Pulmonary toxicity of sodium dichloroisocyanurate after intratracheal instillation in sprague-dawley rats.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, Swedesboro, NJ, USA).

Article Title: ROBO1 protein expression is independently associated with biochemical recurrence in prostate cancer patients who underwent radical prostatectomy in Asian patients
Article Snippet: After 24 h, the cells that penetrated to the underside surface of the inserts were fixed and stained with Diff-Quick (Fisher Scientific, Pittsburgh, PA, USA) and counted and compared to native prostate cancer cells and knockdown cells under light microscopy.

Article Title: Topical Imiquimod Therapy for Localized Solar Dermatitis in a Dog.
Article Snippet: Chronic sun exposure in dogs can result in clinical changes in the skin referred to as solar or actinic dermatitis.. A 5-year-old, neutered male, American Bulldog presented with localized unilateral erythematous macules, plaques, alopecia, comedones and hemorrhagic bullae involving the non-pigmented skin on left lateral ventral flank area.. Thepresence of hyperplastic epidermiswith keratinocyte dysplasia, superficial dermal elastosis, and multiple follicular cysts with occasional rupture (furunculosis) on histology, together with history and characteristic skin lesions was consistent solar dermatitis/actinic keratosis.

Article Title: Comparative effects of human-equivalent low, moderate, and high dose oral prednisone intake on autoimmunity and glucocorticoid-related toxicity in a murine model of environmental-triggered lupus
Article Snippet: Cytological slides were prepared by centrifuging BALF at 400 x g for 10 min using the Shandon Cytospin 3 (Shandon Scientific, PA) and stained with Diff-Quick (Fisher Scientific).

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells
Article Snippet: The slides were stained with Diff‐Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Bacteria:

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Article Title: Subchronic intranasal lipopolysaccharide exposure induces pulmonary autoimmunity and glomerulonephritis in NZBWF1 mice.
Article Snippet: In preparation for differential cell counts, BALF cells were immobilized on glass slides using centrifugal forces (Shandon Cytospin 3) and spun down at 40 x g for 10 min. Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice.
Article Snippet: Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Pulmonary toxicity of sodium dichloroisocyanurate after intratracheal instillation in sprague-dawley rats.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, Swedesboro, NJ, USA).

Article Title: ROBO1 protein expression is independently associated with biochemical recurrence in prostate cancer patients who underwent radical prostatectomy in Asian patients
Article Snippet: After 24 h, the cells that penetrated to the underside surface of the inserts were fixed and stained with Diff-Quick (Fisher Scientific, Pittsburgh, PA, USA) and counted and compared to native prostate cancer cells and knockdown cells under light microscopy.

Article Title: Topical Imiquimod Therapy for Localized Solar Dermatitis in a Dog.
Article Snippet: Chronic sun exposure in dogs can result in clinical changes in the skin referred to as solar or actinic dermatitis.. A 5-year-old, neutered male, American Bulldog presented with localized unilateral erythematous macules, plaques, alopecia, comedones and hemorrhagic bullae involving the non-pigmented skin on left lateral ventral flank area.. Thepresence of hyperplastic epidermiswith keratinocyte dysplasia, superficial dermal elastosis, and multiple follicular cysts with occasional rupture (furunculosis) on histology, together with history and characteristic skin lesions was consistent solar dermatitis/actinic keratosis.

Article Title: Comparative effects of human-equivalent low, moderate, and high dose oral prednisone intake on autoimmunity and glucocorticoid-related toxicity in a murine model of environmental-triggered lupus
Article Snippet: Cytological slides were prepared by centrifuging BALF at 400 x g for 10 min using the Shandon Cytospin 3 (Shandon Scientific, PA) and stained with Diff-Quick (Fisher Scientific).

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells
Article Snippet: The slides were stained with Diff‐Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Knockdown:

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Article Title: Subchronic intranasal lipopolysaccharide exposure induces pulmonary autoimmunity and glomerulonephritis in NZBWF1 mice.
Article Snippet: In preparation for differential cell counts, BALF cells were immobilized on glass slides using centrifugal forces (Shandon Cytospin 3) and spun down at 40 x g for 10 min. Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice.
Article Snippet: Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Pulmonary toxicity of sodium dichloroisocyanurate after intratracheal instillation in sprague-dawley rats.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, Swedesboro, NJ, USA).

Article Title: ROBO1 protein expression is independently associated with biochemical recurrence in prostate cancer patients who underwent radical prostatectomy in Asian patients
Article Snippet: After 24 h, the cells that penetrated to the underside surface of the inserts were fixed and stained with Diff-Quick (Fisher Scientific, Pittsburgh, PA, USA) and counted and compared to native prostate cancer cells and knockdown cells under light microscopy.

Article Title: Topical Imiquimod Therapy for Localized Solar Dermatitis in a Dog.
Article Snippet: Chronic sun exposure in dogs can result in clinical changes in the skin referred to as solar or actinic dermatitis.. A 5-year-old, neutered male, American Bulldog presented with localized unilateral erythematous macules, plaques, alopecia, comedones and hemorrhagic bullae involving the non-pigmented skin on left lateral ventral flank area.. Thepresence of hyperplastic epidermiswith keratinocyte dysplasia, superficial dermal elastosis, and multiple follicular cysts with occasional rupture (furunculosis) on histology, together with history and characteristic skin lesions was consistent solar dermatitis/actinic keratosis.

Article Title: Comparative effects of human-equivalent low, moderate, and high dose oral prednisone intake on autoimmunity and glucocorticoid-related toxicity in a murine model of environmental-triggered lupus
Article Snippet: Cytological slides were prepared by centrifuging BALF at 400 x g for 10 min using the Shandon Cytospin 3 (Shandon Scientific, PA) and stained with Diff-Quick (Fisher Scientific).

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells
Article Snippet: The slides were stained with Diff‐Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Light Microscopy:

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.

Article Title: Subchronic intranasal lipopolysaccharide exposure induces pulmonary autoimmunity and glomerulonephritis in NZBWF1 mice.
Article Snippet: In preparation for differential cell counts, BALF cells were immobilized on glass slides using centrifugal forces (Shandon Cytospin 3) and spun down at 40 x g for 10 min. Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice.
Article Snippet: Cytological slides were air-dried and then stained using Diff-Quick (Fisher Scientific).

Article Title: Pulmonary toxicity of sodium dichloroisocyanurate after intratracheal instillation in sprague-dawley rats.
Article Snippet: The slides were stained with Diff-Quick (Fisher Scientific, Swedesboro, NJ, USA).

Article Title: ROBO1 protein expression is independently associated with biochemical recurrence in prostate cancer patients who underwent radical prostatectomy in Asian patients
Article Snippet: After 24 h, the cells that penetrated to the underside surface of the inserts were fixed and stained with Diff-Quick (Fisher Scientific, Pittsburgh, PA, USA) and counted and compared to native prostate cancer cells and knockdown cells under light microscopy.

Article Title: Topical Imiquimod Therapy for Localized Solar Dermatitis in a Dog.
Article Snippet: Chronic sun exposure in dogs can result in clinical changes in the skin referred to as solar or actinic dermatitis.. A 5-year-old, neutered male, American Bulldog presented with localized unilateral erythematous macules, plaques, alopecia, comedones and hemorrhagic bullae involving the non-pigmented skin on left lateral ventral flank area.. Thepresence of hyperplastic epidermiswith keratinocyte dysplasia, superficial dermal elastosis, and multiple follicular cysts with occasional rupture (furunculosis) on histology, together with history and characteristic skin lesions was consistent solar dermatitis/actinic keratosis.

Article Title: Comparative effects of human-equivalent low, moderate, and high dose oral prednisone intake on autoimmunity and glucocorticoid-related toxicity in a murine model of environmental-triggered lupus
Article Snippet: Cytological slides were prepared by centrifuging BALF at 400 x g for 10 min using the Shandon Cytospin 3 (Shandon Scientific, PA) and stained with Diff-Quick (Fisher Scientific).

Article Title: Model of severe malaria in young mice suggests unique response of CD4 T cells
Article Snippet: The slides were stained with Diff‐Quick (Fisher Scientific, GA) and parasites were counted by microscopy in 10 to 50 different fields depending on the parasite load and day of infection.



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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Image Search Results


RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction