diaminobenzidine  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    3 3 Diaminobenzidine tetrahydrochloride
    Description:

    Catalog Number:
    d5905
    Price:
    None
    Applications:
    Reagent for spectrophotometric determination of selenium.
    Buy from Supplier


    Structured Review

    Millipore diaminobenzidine
    3 3 Diaminobenzidine tetrahydrochloride

    https://www.bioz.com/result/diaminobenzidine/product/Millipore
    Average 99 stars, based on 1462 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "Late-onset Parkinsonism in NF?B/c-Rel-deficient mice"

    Article Title: Late-onset Parkinsonism in NF?B/c-Rel-deficient mice

    Journal: Brain

    doi: 10.1093/brain/aws193

    DMT1 immunoreactivity and iron staining in mesencephalon of 18-month-old c-rel −/− and wild-type mice. Tyrosine hydroxylase staining ( A and B ) and diaminobenzidine enhanced Perl iron staining ( C–F ) in sections of 18-month-old wild-type ( A, C and E ) and c-rel −/− mice ( B, D and F ). Higher magnifications of Perl iron staining are in panels E and F . While the number of tyrosine hydroxylase-positive neurons decreased, the iron staining significantly increased in the substantia nigra pars compacta and reticulata of c-rel −/− mice compared with wild-type mice. Representative immunoblotting of the ferrous iron transporter DMT1 in the striatal and mesencephalic extracts of 18-month-old wild-type and c-rel −/− mice ( G ). Densitometric analysis of DMT1 relative to GAPDH ( H ). DMT1 60 and 90 kDa bands significantly increased in the mesencephalon of c-rel −/− mice (-/-) compared with wild-type animals. Significant difference in the DMT1 90 kDa band was detected in the striatum. Values represent the mean ± SEM ( n = 3 animals per group, * P
    Figure Legend Snippet: DMT1 immunoreactivity and iron staining in mesencephalon of 18-month-old c-rel −/− and wild-type mice. Tyrosine hydroxylase staining ( A and B ) and diaminobenzidine enhanced Perl iron staining ( C–F ) in sections of 18-month-old wild-type ( A, C and E ) and c-rel −/− mice ( B, D and F ). Higher magnifications of Perl iron staining are in panels E and F . While the number of tyrosine hydroxylase-positive neurons decreased, the iron staining significantly increased in the substantia nigra pars compacta and reticulata of c-rel −/− mice compared with wild-type mice. Representative immunoblotting of the ferrous iron transporter DMT1 in the striatal and mesencephalic extracts of 18-month-old wild-type and c-rel −/− mice ( G ). Densitometric analysis of DMT1 relative to GAPDH ( H ). DMT1 60 and 90 kDa bands significantly increased in the mesencephalon of c-rel −/− mice (-/-) compared with wild-type animals. Significant difference in the DMT1 90 kDa band was detected in the striatum. Values represent the mean ± SEM ( n = 3 animals per group, * P

    Techniques Used: Staining, Mouse Assay

    2) Product Images from "Fos Expression in Neurons of the Rat Vestibulo-Autonomic Pathway Activated by Sinusoidal Galvanic Vestibular Stimulation"

    Article Title: Fos Expression in Neurons of the Rat Vestibulo-Autonomic Pathway Activated by Sinusoidal Galvanic Vestibular Stimulation

    Journal: Frontiers in Neurology

    doi: 10.3389/fneur.2012.00004

    Neurons in MVN activated by sGVS, visualized in vibratome sections processed for c-Fos immunoperoxidase/diaminobenzidine staining . The panels illustrate six rostro-caudal levels of the MVN from the same sGVS-stimulated rat. The images were obtained using the same microscopy and imaging conditions, and were subject to the same adjustments of brightness and contrast (see Materials and Methods ). In all panels, the midline is to the left. A dense cluster of immunopositive cells is present in the rostral pole of MVNpc (A,B) . The few activated neurons in MVNmc (A–D) are small diameter cells; none of the larger diameter neurons of this region were c-Fos-positive. c-Fos-stained cells were scattered throughout the caudal spinal vestibular nucleus (B–F) . Approximate Bregma levels are indicated in the upper right of each panel. Abbreviations: MVN, medial vestibular nucleus; MVNmc, medial vestibular nucleus, magnocellular division; MVNpc, medial vestibular nucleus, parvocellular division; NTS, nucleus tractus solitarius; SpVN, spinal vestibular nucleus. Scale bar in (F) represents 100 μm, and is for all panels.
    Figure Legend Snippet: Neurons in MVN activated by sGVS, visualized in vibratome sections processed for c-Fos immunoperoxidase/diaminobenzidine staining . The panels illustrate six rostro-caudal levels of the MVN from the same sGVS-stimulated rat. The images were obtained using the same microscopy and imaging conditions, and were subject to the same adjustments of brightness and contrast (see Materials and Methods ). In all panels, the midline is to the left. A dense cluster of immunopositive cells is present in the rostral pole of MVNpc (A,B) . The few activated neurons in MVNmc (A–D) are small diameter cells; none of the larger diameter neurons of this region were c-Fos-positive. c-Fos-stained cells were scattered throughout the caudal spinal vestibular nucleus (B–F) . Approximate Bregma levels are indicated in the upper right of each panel. Abbreviations: MVN, medial vestibular nucleus; MVNmc, medial vestibular nucleus, magnocellular division; MVNpc, medial vestibular nucleus, parvocellular division; NTS, nucleus tractus solitarius; SpVN, spinal vestibular nucleus. Scale bar in (F) represents 100 μm, and is for all panels.

    Techniques Used: Staining, Microscopy, Imaging

    Representative vibratome sections through the vestibular nuclei from two sGVS-stimulated (A,B) and two mock (non)stimulated (C,D) rats processed for immunoperoxidase/diaminobenzidine staining of c-Fos protein . c-Fos-immunoreactive neuronal nuclei are apparent in the spinal and medial vestibular nuclei (SpVN, MVN), as well as nucleus tractus solitarius (NTS), of the stimulated animals. Sections from the mock-stimulated animals contained c-Fos-labeled cells in NTS, but rarely in the vestibular nuclei. Scale bar in (D) is for all panels.
    Figure Legend Snippet: Representative vibratome sections through the vestibular nuclei from two sGVS-stimulated (A,B) and two mock (non)stimulated (C,D) rats processed for immunoperoxidase/diaminobenzidine staining of c-Fos protein . c-Fos-immunoreactive neuronal nuclei are apparent in the spinal and medial vestibular nuclei (SpVN, MVN), as well as nucleus tractus solitarius (NTS), of the stimulated animals. Sections from the mock-stimulated animals contained c-Fos-labeled cells in NTS, but rarely in the vestibular nuclei. Scale bar in (D) is for all panels.

    Techniques Used: Staining, Labeling

    A vibratome section through the caudal vestibular nuclei from an sGVS-stimulated rat, stained with anti-c-Fos antibody pre-incubated with a peptide blocker and then further processed for immunoperoxidase/diaminobenzidine staining . Signal was negligible in such control sections, and in those in which primary and/or secondary reagents were omitted from the processing protocol.
    Figure Legend Snippet: A vibratome section through the caudal vestibular nuclei from an sGVS-stimulated rat, stained with anti-c-Fos antibody pre-incubated with a peptide blocker and then further processed for immunoperoxidase/diaminobenzidine staining . Signal was negligible in such control sections, and in those in which primary and/or secondary reagents were omitted from the processing protocol.

    Techniques Used: Staining, Incubation

    3) Product Images from "miR-125b promotes cell death by targeting spindle assembly checkpoint gene MAD1 and modulating mitotic progression"

    Article Title: miR-125b promotes cell death by targeting spindle assembly checkpoint gene MAD1 and modulating mitotic progression

    Journal: Cell Death and Differentiation

    doi: 10.1038/cdd.2012.135

    MAD1 expression negatively correlates with that of miR-125b in HNOC. ( a ) Reciprocal relation of miR-125b and MAD1 in HNOC tumours. Total RNA was isolated from primary HNOC tissues ( n =25) and adjacent normal tissues ( n =20). cDNA was prepared by stem-loop primers specific for miR-125b and miR-17-5p and subjected to RT-PCR. Values were normalized to those of miR-17-5p. For MAD1, the above RNA was reverse transcribed and cDNA was subjected to RT-PCR using MAD1 -specific primers. GAPDH was used as endogenous control. ΔΔCt values were calculated and data plotted in terms of log 2 of relative expressions. P -values are indicated. ‘ n ' represents the number of tumour samples and circles represent outliers. ( b ) Representative images showing inverse relation between Mad1 and miR-125b in primary HNOC tissues. Paraffin-embedded tissues samples (in which miR-125b expression was found to be low and Mad1 expression was high and vice versa ; n =16) were processed for IHC with antibody against Mad1, stained with 3,3′diaminobenzidine and counterstained with hematoxylin. Nuclei, which stained mild or deep brown represent moderate or high expression of Mad1, respectively. Blue/bluish-purple staining of nuclei represents low Mad1 expression. Images represent × 20 magnification, while inset images represent × 40 magnification. Scale bars represent 50 μ m. T1, T2, T3, T4, T5 and T6 are representative tumour samples taken from six individual patients
    Figure Legend Snippet: MAD1 expression negatively correlates with that of miR-125b in HNOC. ( a ) Reciprocal relation of miR-125b and MAD1 in HNOC tumours. Total RNA was isolated from primary HNOC tissues ( n =25) and adjacent normal tissues ( n =20). cDNA was prepared by stem-loop primers specific for miR-125b and miR-17-5p and subjected to RT-PCR. Values were normalized to those of miR-17-5p. For MAD1, the above RNA was reverse transcribed and cDNA was subjected to RT-PCR using MAD1 -specific primers. GAPDH was used as endogenous control. ΔΔCt values were calculated and data plotted in terms of log 2 of relative expressions. P -values are indicated. ‘ n ' represents the number of tumour samples and circles represent outliers. ( b ) Representative images showing inverse relation between Mad1 and miR-125b in primary HNOC tissues. Paraffin-embedded tissues samples (in which miR-125b expression was found to be low and Mad1 expression was high and vice versa ; n =16) were processed for IHC with antibody against Mad1, stained with 3,3′diaminobenzidine and counterstained with hematoxylin. Nuclei, which stained mild or deep brown represent moderate or high expression of Mad1, respectively. Blue/bluish-purple staining of nuclei represents low Mad1 expression. Images represent × 20 magnification, while inset images represent × 40 magnification. Scale bars represent 50 μ m. T1, T2, T3, T4, T5 and T6 are representative tumour samples taken from six individual patients

    Techniques Used: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry, Staining

    4) Product Images from "Reactive oxygen species form part of a regulatory pathway initiating trans-differentiation of epidermal transfer cells in Vicia faba cotyledons"

    Article Title: Reactive oxygen species form part of a regulatory pathway initiating trans-differentiation of epidermal transfer cells in Vicia faba cotyledons

    Journal: Journal of Experimental Botany

    doi: 10.1093/jxb/ers029

    Localization of hydrogen peroxide (H 2 O 2 ) in transverse sectioned Vicia faba cotyledons during wall ingrowth induction. Representative light micrographs of transverse sectioned cotyledons following culture for 15 h on MS medium alone (A), or MS medium containing either 100 μM diphenyleneiodonium (DPI; B) or 10 μM hydrogen peroxide (H 2 O 2 ; C). All media contained 1 mg ml −1 diaminobenzidine (DAB) to localize H 2 O 2 detected as H 2 O 2 -induced DAB polymerization visualized as brown precipitates indicated by arrows, noting that (B) exhibits an extremely faint signal. ec, adaxial epidermal cell; sp, storage parenchyma cell. Bar=20 μm.
    Figure Legend Snippet: Localization of hydrogen peroxide (H 2 O 2 ) in transverse sectioned Vicia faba cotyledons during wall ingrowth induction. Representative light micrographs of transverse sectioned cotyledons following culture for 15 h on MS medium alone (A), or MS medium containing either 100 μM diphenyleneiodonium (DPI; B) or 10 μM hydrogen peroxide (H 2 O 2 ; C). All media contained 1 mg ml −1 diaminobenzidine (DAB) to localize H 2 O 2 detected as H 2 O 2 -induced DAB polymerization visualized as brown precipitates indicated by arrows, noting that (B) exhibits an extremely faint signal. ec, adaxial epidermal cell; sp, storage parenchyma cell. Bar=20 μm.

    Techniques Used: Mass Spectrometry

    5) Product Images from "Exosomes taken up by neurons hijack the endosomal pathway to spread to interconnected neurons"

    Article Title: Exosomes taken up by neurons hijack the endosomal pathway to spread to interconnected neurons

    Journal: Acta Neuropathologica Communications

    doi: 10.1186/s40478-018-0514-4

    Hijacking of endogenous endosomes revealed by electron microscopy. Hippocampal neurons in microfluidic devices in Ch1 according to model 2 were treated with exosomes isolated from mouse brains and labeled with FM1–43FX, a fluorescent dye that reacts with diaminobenzidine (DAB) to form insoluble dark precipitates that are visualized by electron microscopy. a Electron microscopy image of a neuronal soma in Ch1, showing that the majority of endosomes contain DAB-positive exosomes of exogenous origin (black arrowheads). A few endosomes are DAB-negative (white arrowheads). b High magnification of endosomes located in the neuronal soma, showing a mixture of exogenous DAB-positive exosomes (black arrowheads) and endogenous DAB-negative intraluminal nanovesicles (black arrows). c Somatic endosome showing engulfment and fusion with a smaller endosome containing DAB-positive exosomes (black arrowhead “ a ”). An exogenous DAB-positive exosome can be seen close to the fused endosome (black arrowhead “ b ”). Endogenous DAB-negative intraluminal nanovesicles are also visualized (black arrows). d-f Endosomes found in axons. (d) Low magnification of axons transporting a small DAB-positive endosome (black arrowhead “ a ”) and a large endosome in front of it (black arrowhead “ b ”). e High magnification of a large axonal endosome containing a mixture of DAB-positive (black arrowheads) and DAB-negative vesicles (black arrows). f Axonal termini showing endosome fusion with the plasma membrane (indicated with “!”) during exosome release and potential residue of the back-fusion with the limiting membrane of the endosome ( * ). Exogenous DAB-positive (black arrowheads) and endogenous DAB-negative exosomes (black arrows). g-i Endosomes found in dendrites. g Low magnification of a dendrite demonstrating the presence of endosomes carrying both DAB-positive exosomes (black arrowheads) and DAB-negative intraluminal nanovesicles (black arrows). h and i High magnification of dendritic endosomes carrying DAB-positive exosomes less than 100 nm diameter (black arrowheads) together with DAB-negative vesicles of a similar size (black arrows). m, mitochondria; n, nucleus
    Figure Legend Snippet: Hijacking of endogenous endosomes revealed by electron microscopy. Hippocampal neurons in microfluidic devices in Ch1 according to model 2 were treated with exosomes isolated from mouse brains and labeled with FM1–43FX, a fluorescent dye that reacts with diaminobenzidine (DAB) to form insoluble dark precipitates that are visualized by electron microscopy. a Electron microscopy image of a neuronal soma in Ch1, showing that the majority of endosomes contain DAB-positive exosomes of exogenous origin (black arrowheads). A few endosomes are DAB-negative (white arrowheads). b High magnification of endosomes located in the neuronal soma, showing a mixture of exogenous DAB-positive exosomes (black arrowheads) and endogenous DAB-negative intraluminal nanovesicles (black arrows). c Somatic endosome showing engulfment and fusion with a smaller endosome containing DAB-positive exosomes (black arrowhead “ a ”). An exogenous DAB-positive exosome can be seen close to the fused endosome (black arrowhead “ b ”). Endogenous DAB-negative intraluminal nanovesicles are also visualized (black arrows). d-f Endosomes found in axons. (d) Low magnification of axons transporting a small DAB-positive endosome (black arrowhead “ a ”) and a large endosome in front of it (black arrowhead “ b ”). e High magnification of a large axonal endosome containing a mixture of DAB-positive (black arrowheads) and DAB-negative vesicles (black arrows). f Axonal termini showing endosome fusion with the plasma membrane (indicated with “!”) during exosome release and potential residue of the back-fusion with the limiting membrane of the endosome ( * ). Exogenous DAB-positive (black arrowheads) and endogenous DAB-negative exosomes (black arrows). g-i Endosomes found in dendrites. g Low magnification of a dendrite demonstrating the presence of endosomes carrying both DAB-positive exosomes (black arrowheads) and DAB-negative intraluminal nanovesicles (black arrows). h and i High magnification of dendritic endosomes carrying DAB-positive exosomes less than 100 nm diameter (black arrowheads) together with DAB-negative vesicles of a similar size (black arrows). m, mitochondria; n, nucleus

    Techniques Used: Electron Microscopy, Isolation, Labeling

    6) Product Images from "DIRECT PROJECTIONS FROM THE CAUDAL VESTIBULAR NUCLEI TO THE VENTROLATERAL MEDULLA IN THE RAT"

    Article Title: DIRECT PROJECTIONS FROM THE CAUDAL VESTIBULAR NUCLEI TO THE VENTROLATERAL MEDULLA IN THE RAT

    Journal: Neuroscience

    doi: 10.1016/j.neuroscience.2010.12.011

    Retrograde injections in the VLM and retrogradely-labeled cells in the VNCc. A. An example of the largest FluoroGold injection in this study, visualized using diaminobenzidine. The injection site was directed at the ventral surface of the brainstem at the caudal pole of RVLM, approximately 12.8 mm caudal to Bregma. Tracer spread was approximately 100 μm rostro-caudally, and 500 μm medio-laterally and dorso-ventrally. Local labeled cell bodies were apparent within the tracer diffusion penumbra. B – D: Examples of retrogradely-labeled globular (B), multipolar (C) and fusiform (D) cells in the spinal vestibular nucleus following a FluoroGold injection in the caudal RVLM. E, F: Examples of FluoroGold-immunofluorescent cells in the periventricular (E) and tegmental (F) portions of the caudal medial vestibular nucleus. Panels B–F are maximum intensity projections of a series of 40x Apotome images collected serially through the z-axis of each neuron. Scale bar in F is for panels B–F. Abbreviations: Amb: nucleus ambiguus; mlf: medial longitudinal fasciculus; MVe: medial vestibular nucleus; RVLM: rostral ventrolateral medulla; Sol: solitary nucleus; SpVe: spinal (inferior) vestibular nucleus.
    Figure Legend Snippet: Retrograde injections in the VLM and retrogradely-labeled cells in the VNCc. A. An example of the largest FluoroGold injection in this study, visualized using diaminobenzidine. The injection site was directed at the ventral surface of the brainstem at the caudal pole of RVLM, approximately 12.8 mm caudal to Bregma. Tracer spread was approximately 100 μm rostro-caudally, and 500 μm medio-laterally and dorso-ventrally. Local labeled cell bodies were apparent within the tracer diffusion penumbra. B – D: Examples of retrogradely-labeled globular (B), multipolar (C) and fusiform (D) cells in the spinal vestibular nucleus following a FluoroGold injection in the caudal RVLM. E, F: Examples of FluoroGold-immunofluorescent cells in the periventricular (E) and tegmental (F) portions of the caudal medial vestibular nucleus. Panels B–F are maximum intensity projections of a series of 40x Apotome images collected serially through the z-axis of each neuron. Scale bar in F is for panels B–F. Abbreviations: Amb: nucleus ambiguus; mlf: medial longitudinal fasciculus; MVe: medial vestibular nucleus; RVLM: rostral ventrolateral medulla; Sol: solitary nucleus; SpVe: spinal (inferior) vestibular nucleus.

    Techniques Used: Labeling, Injection, Diffusion-based Assay

    Representative anterograde tracer injection sites. A. The location and extent of the largest of the anterograde Phaseolus vulgaris ). Darker gray shades indicate greater tracer deposition. The injection was almost entirely restricted to the caudal medial vestibular nucleus (MVe) although some of the penumbra showed diffusion into the adjacent spinal vestibular nucleus (SpVe) caudally and prepositus nucleus (Pr) rostrally. Approximate Bregma coordinates from the published atlas are indicated in the midline of each schematic section. B. PhaL injection placed in the caudal MVe, visualized using diaminobenzidine. Other than a small amount of tracer spread into the adjacent spinal vestibular nucleus, the injection remained within the confines of the medial nucleus. Abbreviations: 8n: vestibulo-cochlear nerve; 12: hypoglossal nucleus; Amb: nucleus ambiguus; DC: dorsal cochlear nucleus; ECu: external cuneate nucleus; icp: inferior cerebellar peduncle; Li: linear nucleus; LVe: lateral vestibular nucleus; mlf: medial longitudinal fasciculus; MVeMC: medial vestibular nucleus, magnocellular division; MVePC: medial vestibular nucleus, parvocellular division; Pr: prepositus nucleus; py: pyramids; RVL: rostral ventrolateral medulla; scp: superior cerebellar peduncle; sol: solitary tract; Sol: solitary nucleus; sp5: spinal trigeminal tract; Sp5I: spinal trigeminal nucleus, pars interpolaris; SpVe: spinal (inferior) vestibular nucleus; SuVe: superior vestibular nucleus; VCP: ventral cochlear nucleus, posterior division.
    Figure Legend Snippet: Representative anterograde tracer injection sites. A. The location and extent of the largest of the anterograde Phaseolus vulgaris ). Darker gray shades indicate greater tracer deposition. The injection was almost entirely restricted to the caudal medial vestibular nucleus (MVe) although some of the penumbra showed diffusion into the adjacent spinal vestibular nucleus (SpVe) caudally and prepositus nucleus (Pr) rostrally. Approximate Bregma coordinates from the published atlas are indicated in the midline of each schematic section. B. PhaL injection placed in the caudal MVe, visualized using diaminobenzidine. Other than a small amount of tracer spread into the adjacent spinal vestibular nucleus, the injection remained within the confines of the medial nucleus. Abbreviations: 8n: vestibulo-cochlear nerve; 12: hypoglossal nucleus; Amb: nucleus ambiguus; DC: dorsal cochlear nucleus; ECu: external cuneate nucleus; icp: inferior cerebellar peduncle; Li: linear nucleus; LVe: lateral vestibular nucleus; mlf: medial longitudinal fasciculus; MVeMC: medial vestibular nucleus, magnocellular division; MVePC: medial vestibular nucleus, parvocellular division; Pr: prepositus nucleus; py: pyramids; RVL: rostral ventrolateral medulla; scp: superior cerebellar peduncle; sol: solitary tract; Sol: solitary nucleus; sp5: spinal trigeminal tract; Sp5I: spinal trigeminal nucleus, pars interpolaris; SpVe: spinal (inferior) vestibular nucleus; SuVe: superior vestibular nucleus; VCP: ventral cochlear nucleus, posterior division.

    Techniques Used: Injection, Diffusion-based Assay

    7) Product Images from "Exosomes taken up by neurons hijack the endosomal pathway to spread to interconnected neurons"

    Article Title: Exosomes taken up by neurons hijack the endosomal pathway to spread to interconnected neurons

    Journal: Acta Neuropathologica Communications

    doi: 10.1186/s40478-018-0514-4

    Hijacking of endogenous endosomes revealed by electron microscopy. Hippocampal neurons in microfluidic devices in Ch1 according to model 2 were treated with exosomes isolated from mouse brains and labeled with FM1–43FX, a fluorescent dye that reacts with diaminobenzidine (DAB) to form insoluble dark precipitates that are visualized by electron microscopy. a Electron microscopy image of a neuronal soma in Ch1, showing that the majority of endosomes contain DAB-positive exosomes of exogenous origin (black arrowheads). A few endosomes are DAB-negative (white arrowheads). b High magnification of endosomes located in the neuronal soma, showing a mixture of exogenous DAB-positive exosomes (black arrowheads) and endogenous DAB-negative intraluminal nanovesicles (black arrows). c Somatic endosome showing engulfment and fusion with a smaller endosome containing DAB-positive exosomes (black arrowhead “ a ”). An exogenous DAB-positive exosome can be seen close to the fused endosome (black arrowhead “ b ”). Endogenous DAB-negative intraluminal nanovesicles are also visualized (black arrows). d-f Endosomes found in axons. (d) Low magnification of axons transporting a small DAB-positive endosome (black arrowhead “ a ”) and a large endosome in front of it (black arrowhead “ b ”). e High magnification of a large axonal endosome containing a mixture of DAB-positive (black arrowheads) and DAB-negative vesicles (black arrows). f Axonal termini showing endosome fusion with the plasma membrane (indicated with “!”) during exosome release and potential residue of the back-fusion with the limiting membrane of the endosome ( * ). Exogenous DAB-positive (black arrowheads) and endogenous DAB-negative exosomes (black arrows). g-i Endosomes found in dendrites. g Low magnification of a dendrite demonstrating the presence of endosomes carrying both DAB-positive exosomes (black arrowheads) and DAB-negative intraluminal nanovesicles (black arrows). h and i High magnification of dendritic endosomes carrying DAB-positive exosomes less than 100 nm diameter (black arrowheads) together with DAB-negative vesicles of a similar size (black arrows). m, mitochondria; n, nucleus
    Figure Legend Snippet: Hijacking of endogenous endosomes revealed by electron microscopy. Hippocampal neurons in microfluidic devices in Ch1 according to model 2 were treated with exosomes isolated from mouse brains and labeled with FM1–43FX, a fluorescent dye that reacts with diaminobenzidine (DAB) to form insoluble dark precipitates that are visualized by electron microscopy. a Electron microscopy image of a neuronal soma in Ch1, showing that the majority of endosomes contain DAB-positive exosomes of exogenous origin (black arrowheads). A few endosomes are DAB-negative (white arrowheads). b High magnification of endosomes located in the neuronal soma, showing a mixture of exogenous DAB-positive exosomes (black arrowheads) and endogenous DAB-negative intraluminal nanovesicles (black arrows). c Somatic endosome showing engulfment and fusion with a smaller endosome containing DAB-positive exosomes (black arrowhead “ a ”). An exogenous DAB-positive exosome can be seen close to the fused endosome (black arrowhead “ b ”). Endogenous DAB-negative intraluminal nanovesicles are also visualized (black arrows). d-f Endosomes found in axons. (d) Low magnification of axons transporting a small DAB-positive endosome (black arrowhead “ a ”) and a large endosome in front of it (black arrowhead “ b ”). e High magnification of a large axonal endosome containing a mixture of DAB-positive (black arrowheads) and DAB-negative vesicles (black arrows). f Axonal termini showing endosome fusion with the plasma membrane (indicated with “!”) during exosome release and potential residue of the back-fusion with the limiting membrane of the endosome ( * ). Exogenous DAB-positive (black arrowheads) and endogenous DAB-negative exosomes (black arrows). g-i Endosomes found in dendrites. g Low magnification of a dendrite demonstrating the presence of endosomes carrying both DAB-positive exosomes (black arrowheads) and DAB-negative intraluminal nanovesicles (black arrows). h and i High magnification of dendritic endosomes carrying DAB-positive exosomes less than 100 nm diameter (black arrowheads) together with DAB-negative vesicles of a similar size (black arrows). m, mitochondria; n, nucleus

    Techniques Used: Electron Microscopy, Isolation, Labeling

    8) Product Images from "Neuroinflammation and Behavior in HIV-1 Transgenic Rats Exposed to Chronic Adolescent Stress"

    Article Title: Neuroinflammation and Behavior in HIV-1 Transgenic Rats Exposed to Chronic Adolescent Stress

    Journal: Frontiers in Psychiatry

    doi: 10.3389/fpsyt.2016.00102

    HIV-1 transgenic and wild-type rats were exposed to chronic adolescent stress or left non-stressed during adolescence . Brains were sectioned at 40 μm and stained for IBA-1 and visualized with diaminobenzidine. Representative images of the hippocampus for HIV-1 Tg, WT, stressed, and non-stressed rats are shown (A) . Images were adjusted for brightness and contrast. Scale bar = 50 μm. IBA-1-stained microglia were then converted to 8-bit, adjusted for brightness, and cleaned with a Gaussian filter. Images were converted to binary and skeletonized in ImageJ. Representative images of non-stressed WT, stressed WT, non-stressed Tg, and stressed Tg rats are shown (B) .
    Figure Legend Snippet: HIV-1 transgenic and wild-type rats were exposed to chronic adolescent stress or left non-stressed during adolescence . Brains were sectioned at 40 μm and stained for IBA-1 and visualized with diaminobenzidine. Representative images of the hippocampus for HIV-1 Tg, WT, stressed, and non-stressed rats are shown (A) . Images were adjusted for brightness and contrast. Scale bar = 50 μm. IBA-1-stained microglia were then converted to 8-bit, adjusted for brightness, and cleaned with a Gaussian filter. Images were converted to binary and skeletonized in ImageJ. Representative images of non-stressed WT, stressed WT, non-stressed Tg, and stressed Tg rats are shown (B) .

    Techniques Used: Transgenic Assay, Staining

    9) Product Images from "Guttation capsules containing hydrogen peroxide: an evolutionarily conserved NADPH oxidase gains a role in wars between related fungi"

    Article Title: Guttation capsules containing hydrogen peroxide: an evolutionarily conserved NADPH oxidase gains a role in wars between related fungi

    Journal: Environmental Microbiology

    doi: 10.1111/1462-2920.14575

    Production of ROS by Tgui and Foc4 during the dual confrontation assays on GSM. Superoxide is stained in dark blue, and H 2 O 2 is stained in reddish brown. At sixth day, yellow and green lines indicate the extension of Tgui and Foc4 colonies respectively. O 2 •− is stained dark blue by nitro blue tetrazolium, H 2 O 2 is visualized as the reddish brown colour that developed due to the presence of diaminobenzidine and horseradish peroxidase. The O 2 •− accumulation appeared to be independent on the interaction between the two fungi and specific to the feeding (substrate) hyphae. The production of H 2 O 2 was associated with aerial hyphae when Tgui contacted and overgrown Foc4. Petri plate diameter is 9 cm. [Color figure can be viewed at http://wileyonlinelibrary.com ]
    Figure Legend Snippet: Production of ROS by Tgui and Foc4 during the dual confrontation assays on GSM. Superoxide is stained in dark blue, and H 2 O 2 is stained in reddish brown. At sixth day, yellow and green lines indicate the extension of Tgui and Foc4 colonies respectively. O 2 •− is stained dark blue by nitro blue tetrazolium, H 2 O 2 is visualized as the reddish brown colour that developed due to the presence of diaminobenzidine and horseradish peroxidase. The O 2 •− accumulation appeared to be independent on the interaction between the two fungi and specific to the feeding (substrate) hyphae. The production of H 2 O 2 was associated with aerial hyphae when Tgui contacted and overgrown Foc4. Petri plate diameter is 9 cm. [Color figure can be viewed at http://wileyonlinelibrary.com ]

    Techniques Used: Staining

    10) Product Images from "Revascularization of the graft in obliterative bronchiolitis after heterotopic tracheal transplantation. Revascularization of the graft in obliterative bronchiolitis after heterotopic tracheal transplantation"

    Article Title: Revascularization of the graft in obliterative bronchiolitis after heterotopic tracheal transplantation. Revascularization of the graft in obliterative bronchiolitis after heterotopic tracheal transplantation

    Journal: Physiological Reports

    doi: 10.14814/phy2.12690

    Functional vascularization of iso‐and allografts after heterotopic tracheal transplantation. Biotinylated dextran (80 mg/kg) was administered IV before tracheal harvest. (A) Blood vessels were visualized in the tracheal tissue (D0–D21, iso‐ and allograft) and in the fibroproliferative tissue obstructing the tracheal lumen (D21, allograft) after streptavidin‐peroxidase incubation of the paraffin‐embedded sections and were then stained brown with 3,3′‐Diaminobenzidine (dark arrows). Cell nuclei are colored blue after Methyl Green staining. Gx400. (B) Count of dextran + vessels per mm 2 in the tracheal tissue (Tr; Black and white blocks). The count of Dextran + vessels per mm 2 in the fibroproliferative tissue (Fp; Pattern block) is presented for the D21 allograft. Three levels of sections were counted for each trachea. Data represent mean values (blocks) ± SEM (bars) ( n = 6). *** P
    Figure Legend Snippet: Functional vascularization of iso‐and allografts after heterotopic tracheal transplantation. Biotinylated dextran (80 mg/kg) was administered IV before tracheal harvest. (A) Blood vessels were visualized in the tracheal tissue (D0–D21, iso‐ and allograft) and in the fibroproliferative tissue obstructing the tracheal lumen (D21, allograft) after streptavidin‐peroxidase incubation of the paraffin‐embedded sections and were then stained brown with 3,3′‐Diaminobenzidine (dark arrows). Cell nuclei are colored blue after Methyl Green staining. Gx400. (B) Count of dextran + vessels per mm 2 in the tracheal tissue (Tr; Black and white blocks). The count of Dextran + vessels per mm 2 in the fibroproliferative tissue (Fp; Pattern block) is presented for the D21 allograft. Three levels of sections were counted for each trachea. Data represent mean values (blocks) ± SEM (bars) ( n = 6). *** P

    Techniques Used: Functional Assay, Transplantation Assay, Incubation, Staining, Blocking Assay

    11) Product Images from "PROJECTION NEURONS OF THE VESTIBULO-SYMPATHETIC REFLEX PATHWAY"

    Article Title: PROJECTION NEURONS OF THE VESTIBULO-SYMPATHETIC REFLEX PATHWAY

    Journal: The Journal of comparative neurology

    doi: 10.1002/cne.23517

    Vibratome sections from an sGVS-stimulated (A) and a non-stimulated (B) rat, processed identically and contemporaneously for immunoperoxidase/diaminobenzidine staining of c-Fos protein. A: The sGVS stimulation resulted in substantial accumulation of c-Fos
    Figure Legend Snippet: Vibratome sections from an sGVS-stimulated (A) and a non-stimulated (B) rat, processed identically and contemporaneously for immunoperoxidase/diaminobenzidine staining of c-Fos protein. A: The sGVS stimulation resulted in substantial accumulation of c-Fos

    Techniques Used: Staining

    Examples of injection site maps. The location of the injection site and extent of the local diffusion of tracer was identified in each animal using immunoperoxidase/diaminobenzidine-stained sections through the medulla. This site was then plotted on drawings
    Figure Legend Snippet: Examples of injection site maps. The location of the injection site and extent of the local diffusion of tracer was identified in each animal using immunoperoxidase/diaminobenzidine-stained sections through the medulla. This site was then plotted on drawings

    Techniques Used: Injection, Diffusion-based Assay, Staining

    Photomicrographs of immunoperoxidase/diaminobenzidine-stained Vibratome sections from two rats, one with a FluoroGold tracer injection into RVLM (A) and the other with a similar tracer injection into CVLM (B). Estimated Bregma levels are based on matching
    Figure Legend Snippet: Photomicrographs of immunoperoxidase/diaminobenzidine-stained Vibratome sections from two rats, one with a FluoroGold tracer injection into RVLM (A) and the other with a similar tracer injection into CVLM (B). Estimated Bregma levels are based on matching

    Techniques Used: Staining, Injection

    Immunoperoxidase/diaminobenzidine-stained Vibratome sections through the caudal vestibular nuclei illustrating the three morphological types of vestibular neurons that were retrogradely-filled following a FluoroGold tracer injection into CVLM. Multipolar
    Figure Legend Snippet: Immunoperoxidase/diaminobenzidine-stained Vibratome sections through the caudal vestibular nuclei illustrating the three morphological types of vestibular neurons that were retrogradely-filled following a FluoroGold tracer injection into CVLM. Multipolar

    Techniques Used: Staining, Injection

    Related Articles

    Staining:

    Article Title: Attenuated Salmonella enteritidis E23 as a vehicle for the rectal delivery of DNA vaccine coding for HIV-1 polyepitope CTL immunogen
    Article Snippet: .. The HRP complex was visualized by staining with a substrate solution containing 3.3′‐diaminobenzidine tetrahydrochloride (Sigma). ..

    Incubation:

    Article Title: Genetic and Molecular Characterization of Submergence Response Identifies Subtol6 as a Major Submergence Tolerance Locus in Maize
    Article Snippet: .. For H2 O2 visualization, leaf tissue was collected as described above, immersed in 1 mg/mL 3,3-diaminobenzidine tetrahydrochloride (DAB; Sigma-Aldrich, St. Louis, MO, USA) in 50 mM tris-acetate buffer (pH 5.0), and were incubated at 25ºC for 24 h in complete darkness. ..

    Article Title: Fos Expression in Neurons of the Rat Vestibulo-Autonomic Pathway Activated by Sinusoidal Galvanic Vestibular Stimulation
    Article Snippet: .. Sections were then rinsed in PBS (six changes over 2 h), and incubated in Tris buffer (pH 7.6) containing 0.5 mg/ml diaminobenzidine (DAB; Sigma D-5905; St. Louis, MO, USA) with 0.01% H2 O2 . .. Microscopy, stain analysis, and image preparation Sections were examined and images were acquired with a Zeiss Axioplan2 microscope equipped for structured illumination (ApoTome).

    Binding Assay:

    Article Title: Prevalence of small testicular hyperechogenic foci in subgroups of 382 non‐vasectomized, azoospermic men: a retrospective cohort study
    Article Snippet: .. Horseradish peroxidise–avidin biotin complex (HRP‐sABC, DAKO, K 0377) was used for identification of the antigen–antibody binding sites, and the peroxidase activity was visualized using a 0.05% solution of diaminobenzidine tetrahydrochloride (DAB, Sigma D‐5637, 15 min). ..

    Activity Assay:

    Article Title: Prevalence of small testicular hyperechogenic foci in subgroups of 382 non‐vasectomized, azoospermic men: a retrospective cohort study
    Article Snippet: .. Horseradish peroxidise–avidin biotin complex (HRP‐sABC, DAKO, K 0377) was used for identification of the antigen–antibody binding sites, and the peroxidase activity was visualized using a 0.05% solution of diaminobenzidine tetrahydrochloride (DAB, Sigma D‐5637, 15 min). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore 3 3 diaminobenzidine dab
    Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with <t>diaminobenzidine</t> <t>(DAB)</t> for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.
    3 3 Diaminobenzidine Dab, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine dab/product/Millipore
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine dab - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    88
    Millipore diaminobenzidine dab stainings
    Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with <t>diaminobenzidine</t> <t>(DAB)</t> for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.
    Diaminobenzidine Dab Stainings, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diaminobenzidine dab stainings/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diaminobenzidine dab stainings - by Bioz Stars, 2020-11
    88/100 stars
      Buy from Supplier

    Image Search Results


    Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with diaminobenzidine (DAB) for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.

    Journal: Frontiers in Neuroanatomy

    Article Title: Putaminal Mosaic Visualized by Tyrosine Hydroxylase Immunohistochemistry in the Human Neostriatum

    doi: 10.3389/fnana.2016.00034

    Figure Lengend Snippet: Non-homogeneous distribution of TH in the human neostriatum. (A,B) Bright-field (A) and dark-field (B) images taken from a frontal section of the striatum stained with diaminobenzidine (DAB) for TH. (C,D) Bright-field (C) and dark-field (D) images taken from a frontal section of the striatum stained with DAB for [Met]-enkephalin (MEnk), a marker for striosomes (patches). Note that they are contiguous to those shown in (A,B) . Abbreviations: CN, caudate nucleus; Put, putamen; NA, nucleus accumbens. Scale bars: 5 mm.

    Article Snippet: IHC with 3,3′-Diaminobenzidine (DAB) in Human Brain Tissue The sections were incubated for 18 h in 3% BSA-PBS containing a rabbit polyclonal antibody against TH (1:200,000; Sato et al., ) or [Met]-enkephalin (MEnk; 1:500,000; Millipore, St. Louis, MO, USA; Goto et al., ).

    Techniques: Staining, Marker

    Immunohistochemical detection of eTeNT.EGFP in the double-infected L2–L5 interneurons. a – d Volumetric three-dimensional renderings show eTeNT.EGFP + terminals closely apposed to somata and primary dendrites of neurons in the intermediate gray matter (#,* indicate same neuron rotated, x – y – z axes shown in gray/magenta/blue). e Isotype control showed little to no signal (nonimmune rabbit sera at 5 mg/ml, 1:5000 dilution). f – h Volume rendered images show eTeNT.EGFP+ terminals surrounding motor neurons marked by vesicular acetylcholine transferase (VAChT, magenta) in the caudal lumbar segments. Cross-sections throughout the z -stack confirm colocalization of VAChT with eTeNT.EGFP in the x – z and y – z planes ( i – l , white signal in crosshairs; cross-sections throughout motor neuron shown in ( f ); a – f scale = 10 µm). Amplification of eTeNT.EGFP signal with 3,3′-Diaminobenzidine (DAB) revealed double-infected neurons within the intermediate gray matter of the L2 spinal segment ( m – q dark arrowheads = eTeNT.EGFP-positive neurons; white arrowheads = eTeNT.EGFP-negative neurons). r , s Isotype controls revealed no DAB-enhanced eTeNT.EGFP signal. m – o , r , s scale = 100 µm; p , q scale = 50 µm

    Journal: Nature Communications

    Article Title: Reversible silencing of lumbar spinal interneurons unmasks a task-specific network for securing hindlimb alternation

    doi: 10.1038/s41467-017-02033-x

    Figure Lengend Snippet: Immunohistochemical detection of eTeNT.EGFP in the double-infected L2–L5 interneurons. a – d Volumetric three-dimensional renderings show eTeNT.EGFP + terminals closely apposed to somata and primary dendrites of neurons in the intermediate gray matter (#,* indicate same neuron rotated, x – y – z axes shown in gray/magenta/blue). e Isotype control showed little to no signal (nonimmune rabbit sera at 5 mg/ml, 1:5000 dilution). f – h Volume rendered images show eTeNT.EGFP+ terminals surrounding motor neurons marked by vesicular acetylcholine transferase (VAChT, magenta) in the caudal lumbar segments. Cross-sections throughout the z -stack confirm colocalization of VAChT with eTeNT.EGFP in the x – z and y – z planes ( i – l , white signal in crosshairs; cross-sections throughout motor neuron shown in ( f ); a – f scale = 10 µm). Amplification of eTeNT.EGFP signal with 3,3′-Diaminobenzidine (DAB) revealed double-infected neurons within the intermediate gray matter of the L2 spinal segment ( m – q dark arrowheads = eTeNT.EGFP-positive neurons; white arrowheads = eTeNT.EGFP-negative neurons). r , s Isotype controls revealed no DAB-enhanced eTeNT.EGFP signal. m – o , r , s scale = 100 µm; p , q scale = 50 µm

    Article Snippet: Sections were rinsed with 0.1 M PBS (pH 7.4) for two, 5 min washes and then treated with 3% hydrogen peroxide for 10 min. We then used the 3,3′-Diaminobenzidine (DAB) IHC Select kit (DAB500, Millipore), but modified the company protocol (increased the duration of blocking, secondary antibody, streptavidin horseradish peroxidase, and DAB incubations to 10 min each).

    Techniques: Immunohistochemistry, Infection, Amplification

    BACE1 and BACE2 are increased in neurodegenerative disease. A: BACE1 activity (as determined by the MAB931 capture assay) is higher in PCAD, AD, and FTD. B: BACE2 activity (as determined by the Ab1 capture assay) is higher in FTD and PCAD and strongly

    Journal: The American Journal of Pathology

    Article Title: BACE2 Expression Increases in Human Neurodegenerative Disease

    doi: 10.1016/j.ajpath.2011.09.034

    Figure Lengend Snippet: BACE1 and BACE2 are increased in neurodegenerative disease. A: BACE1 activity (as determined by the MAB931 capture assay) is higher in PCAD, AD, and FTD. B: BACE2 activity (as determined by the Ab1 capture assay) is higher in FTD and PCAD and strongly

    Article Snippet: Supplemental Figure S3: Neither BACE1 ( top panel: MAB931, R & D Systems; 3,3′-diaminobenzidine, brown) nor BACE2 ( bottom panel: Ab2, EMD Biosciences; 3,3′-diaminobenzidine, brown) were detected in microglia (rabbit anti-Iba1, Biocare Medical; Vector SG, blue) in significant amounts; note the numerous BACE1- and BACE2-negative microglia in both panels.

    Techniques: Activity Assay

    BACE2 was observed in neurons and astrocytes. Most BACE2 immunoreactivity was found in the cortical layer near the surface of the brain and in areas surrounding blood vessels. A: BACE1 was not seen in astrocytes, even in areas where substantial numbers

    Journal: The American Journal of Pathology

    Article Title: BACE2 Expression Increases in Human Neurodegenerative Disease

    doi: 10.1016/j.ajpath.2011.09.034

    Figure Lengend Snippet: BACE2 was observed in neurons and astrocytes. Most BACE2 immunoreactivity was found in the cortical layer near the surface of the brain and in areas surrounding blood vessels. A: BACE1 was not seen in astrocytes, even in areas where substantial numbers

    Article Snippet: Supplemental Figure S3: Neither BACE1 ( top panel: MAB931, R & D Systems; 3,3′-diaminobenzidine, brown) nor BACE2 ( bottom panel: Ab2, EMD Biosciences; 3,3′-diaminobenzidine, brown) were detected in microglia (rabbit anti-Iba1, Biocare Medical; Vector SG, blue) in significant amounts; note the numerous BACE1- and BACE2-negative microglia in both panels.

    Techniques:

    BACE1 and BACE2 enzymatic activities are related to soluble, but not insoluble, Aβ in the brain. BACE1 ( A ) and BACE2 ( B ) enzymatic activities were significantly correlated with the amount of Aβ solubilized in the NaOAc fraction, which

    Journal: The American Journal of Pathology

    Article Title: BACE2 Expression Increases in Human Neurodegenerative Disease

    doi: 10.1016/j.ajpath.2011.09.034

    Figure Lengend Snippet: BACE1 and BACE2 enzymatic activities are related to soluble, but not insoluble, Aβ in the brain. BACE1 ( A ) and BACE2 ( B ) enzymatic activities were significantly correlated with the amount of Aβ solubilized in the NaOAc fraction, which

    Article Snippet: Supplemental Figure S3: Neither BACE1 ( top panel: MAB931, R & D Systems; 3,3′-diaminobenzidine, brown) nor BACE2 ( bottom panel: Ab2, EMD Biosciences; 3,3′-diaminobenzidine, brown) were detected in microglia (rabbit anti-Iba1, Biocare Medical; Vector SG, blue) in significant amounts; note the numerous BACE1- and BACE2-negative microglia in both panels.

    Techniques:

    BACE1 ( A ) and BACE2 ( B ) are abundant in human brain. Equal amounts of protein from the SMTG (25 μg) were separated by SDS-PAGE under reducing conditions on Criterion 10% to 20% Tris-glycine gels (Bio-Rad Laboratories). Controls included human

    Journal: The American Journal of Pathology

    Article Title: BACE2 Expression Increases in Human Neurodegenerative Disease

    doi: 10.1016/j.ajpath.2011.09.034

    Figure Lengend Snippet: BACE1 ( A ) and BACE2 ( B ) are abundant in human brain. Equal amounts of protein from the SMTG (25 μg) were separated by SDS-PAGE under reducing conditions on Criterion 10% to 20% Tris-glycine gels (Bio-Rad Laboratories). Controls included human

    Article Snippet: Supplemental Figure S3: Neither BACE1 ( top panel: MAB931, R & D Systems; 3,3′-diaminobenzidine, brown) nor BACE2 ( bottom panel: Ab2, EMD Biosciences; 3,3′-diaminobenzidine, brown) were detected in microglia (rabbit anti-Iba1, Biocare Medical; Vector SG, blue) in significant amounts; note the numerous BACE1- and BACE2-negative microglia in both panels.

    Techniques: SDS Page

    BACE1 and BACE2 enzymatic activities and protein levels are not increased in DS. A: As expected, total mRNA levels for BACE2 and APP were significantly higher in DS cases; BACE1 mRNA was unchanged (data not shown). DS cases had slightly more APP protein

    Journal: The American Journal of Pathology

    Article Title: BACE2 Expression Increases in Human Neurodegenerative Disease

    doi: 10.1016/j.ajpath.2011.09.034

    Figure Lengend Snippet: BACE1 and BACE2 enzymatic activities and protein levels are not increased in DS. A: As expected, total mRNA levels for BACE2 and APP were significantly higher in DS cases; BACE1 mRNA was unchanged (data not shown). DS cases had slightly more APP protein

    Article Snippet: Supplemental Figure S3: Neither BACE1 ( top panel: MAB931, R & D Systems; 3,3′-diaminobenzidine, brown) nor BACE2 ( bottom panel: Ab2, EMD Biosciences; 3,3′-diaminobenzidine, brown) were detected in microglia (rabbit anti-Iba1, Biocare Medical; Vector SG, blue) in significant amounts; note the numerous BACE1- and BACE2-negative microglia in both panels.

    Techniques:

    BACE1 ( left panels ) and BACE2 ( right panels ) show distinct patterns of cellular immunoreactivity in the human brain. A and C: BACE1 and BACE2 immunoreactivity was more prevalent in AD brain. Strongly BACE1-positive cells (brown) of distinct neuronal morphology

    Journal: The American Journal of Pathology

    Article Title: BACE2 Expression Increases in Human Neurodegenerative Disease

    doi: 10.1016/j.ajpath.2011.09.034

    Figure Lengend Snippet: BACE1 ( left panels ) and BACE2 ( right panels ) show distinct patterns of cellular immunoreactivity in the human brain. A and C: BACE1 and BACE2 immunoreactivity was more prevalent in AD brain. Strongly BACE1-positive cells (brown) of distinct neuronal morphology

    Article Snippet: Supplemental Figure S3: Neither BACE1 ( top panel: MAB931, R & D Systems; 3,3′-diaminobenzidine, brown) nor BACE2 ( bottom panel: Ab2, EMD Biosciences; 3,3′-diaminobenzidine, brown) were detected in microglia (rabbit anti-Iba1, Biocare Medical; Vector SG, blue) in significant amounts; note the numerous BACE1- and BACE2-negative microglia in both panels.

    Techniques:

    The mRNA for the splice form of BACE2 missing exon 7 (splice form C, or BACE Δ7 ) is increased in neurodegenerative disease. A: The BACE2 Δ7 mRNA expression increased with disease state ( P

    Journal: The American Journal of Pathology

    Article Title: BACE2 Expression Increases in Human Neurodegenerative Disease

    doi: 10.1016/j.ajpath.2011.09.034

    Figure Lengend Snippet: The mRNA for the splice form of BACE2 missing exon 7 (splice form C, or BACE Δ7 ) is increased in neurodegenerative disease. A: The BACE2 Δ7 mRNA expression increased with disease state ( P

    Article Snippet: Supplemental Figure S3: Neither BACE1 ( top panel: MAB931, R & D Systems; 3,3′-diaminobenzidine, brown) nor BACE2 ( bottom panel: Ab2, EMD Biosciences; 3,3′-diaminobenzidine, brown) were detected in microglia (rabbit anti-Iba1, Biocare Medical; Vector SG, blue) in significant amounts; note the numerous BACE1- and BACE2-negative microglia in both panels.

    Techniques: Expressing