di 8 aneppq  (Biotium)

Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet: Imaging data of Di-8-ANEPPQ labeled primary neuronal and cardiac cells in vitro The Di-8-ANEPPQ dye labeling efficiency was determined by flourescent imaging of the primary NG neurons (A) or primary neonatal cardiomyocytes (B) imaged by floresence microscopy. Di-8-ANEPPQ: green, scale bar: 100 µm.

Article Snippet: Description of data collection , For evaluation of in vitro labeling capacity of Di-8-ANEPPQ, primary cultures of NG neurons and cardiomyocytes were stained with 20 µM Di-8-ANEPPQ at RT. After incubation, live images of cultured cells were taken using spinning disk confocal microscopy (Zeiss). The excitation and emission properties of the Di-8-ANEPPQ was obtained using the power spectrum module of confocal microscopy (LSM 780, Zeiss). The images of the in vivo heart operation were captured using the stereomicroscope (Discovery 8, Zeiss). The Di-8-ANEPPQ, in 1 µl containing 10 mg/ml, was injected into the apex of the heart. Following 2-3 hrs of the surgery, the heart was dissected out and the injection area was imaged using confocal microscopy (LSM 780, Zeiss). All images were analyzed and prepared using Fiji software..

Techniques: Imaging, Labeling, In Vitro, Microscopy

Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet: Emission spectrum analysis of Di-8-ANEPPQ labeled primary cell culture. Di-8-ANEPPQ (gray) labeled cardiac cells were excited (A) 488 nm, (B) 561 nm, and (C) 633 nm. Emission spectrum data between 415 nm and 690 nm were captured and displayed. Scale bars: 100 µm.

Article Snippet: Description of data collection , For evaluation of in vitro labeling capacity of Di-8-ANEPPQ, primary cultures of NG neurons and cardiomyocytes were stained with 20 µM Di-8-ANEPPQ at RT. After incubation, live images of cultured cells were taken using spinning disk confocal microscopy (Zeiss). The excitation and emission properties of the Di-8-ANEPPQ was obtained using the power spectrum module of confocal microscopy (LSM 780, Zeiss). The images of the in vivo heart operation were captured using the stereomicroscope (Discovery 8, Zeiss). The Di-8-ANEPPQ, in 1 µl containing 10 mg/ml, was injected into the apex of the heart. Following 2-3 hrs of the surgery, the heart was dissected out and the injection area was imaged using confocal microscopy (LSM 780, Zeiss). All images were analyzed and prepared using Fiji software..

Techniques: Labeling, Cell Culture

Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet: Experimental steps for application of a retrograde dye to the heart in vivo. (A) The anesthetized mouse was shaved at the operation area and the skin was aseptically cleaned. (B) The mouse was intubated using a cannula and connected to the ventilation apparatus. (C) The incisions were made at the left side of the thorax above the xiphoid, and muscles were moved gently to expose the heart. (D) The retractors were placed to separate the third and fourth ribs. (E) T he Di-8-ANEPPQ was injected into apex or (F) DiI paste was applied on the heart. Arrow showed the tissue after DiI paste was applied. (G) The application area observed by the spread of the retrograde dye in the cardiac tissue. The injection site is shown by the arrow. (H) The intercostal muscles and (I) the skin were sutured.

Article Snippet: Description of data collection , For evaluation of in vitro labeling capacity of Di-8-ANEPPQ, primary cultures of NG neurons and cardiomyocytes were stained with 20 µM Di-8-ANEPPQ at RT. After incubation, live images of cultured cells were taken using spinning disk confocal microscopy (Zeiss). The excitation and emission properties of the Di-8-ANEPPQ was obtained using the power spectrum module of confocal microscopy (LSM 780, Zeiss). The images of the in vivo heart operation were captured using the stereomicroscope (Discovery 8, Zeiss). The Di-8-ANEPPQ, in 1 µl containing 10 mg/ml, was injected into the apex of the heart. Following 2-3 hrs of the surgery, the heart was dissected out and the injection area was imaged using confocal microscopy (LSM 780, Zeiss). All images were analyzed and prepared using Fiji software..

Techniques: In Vivo, Injection

Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet: Imaging data of Di-8-ANEPPQ from in vivo labeled cardiac tissue . Fluorescence imaging was performed on unlabeled control heart tissue for determining (A) the background or (B) following the Di-8-ANEPPQ administration to the murine heart in vivo by confocal microcopy. Di-8-ANEPPQ: green. Scale bar: 100 µm.

Article Snippet: Description of data collection , For evaluation of in vitro labeling capacity of Di-8-ANEPPQ, primary cultures of NG neurons and cardiomyocytes were stained with 20 µM Di-8-ANEPPQ at RT. After incubation, live images of cultured cells were taken using spinning disk confocal microscopy (Zeiss). The excitation and emission properties of the Di-8-ANEPPQ was obtained using the power spectrum module of confocal microscopy (LSM 780, Zeiss). The images of the in vivo heart operation were captured using the stereomicroscope (Discovery 8, Zeiss). The Di-8-ANEPPQ, in 1 µl containing 10 mg/ml, was injected into the apex of the heart. Following 2-3 hrs of the surgery, the heart was dissected out and the injection area was imaged using confocal microscopy (LSM 780, Zeiss). All images were analyzed and prepared using Fiji software..

Techniques: Imaging, In Vivo, Labeling, Fluorescence

Journal: Data in Brief

Article Title: Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

doi: 10.1016/j.dib.2021.106834

Figure Lengend Snippet:

Article Snippet: Description of data collection , For evaluation of in vitro labeling capacity of Di-8-ANEPPQ, primary cultures of NG neurons and cardiomyocytes were stained with 20 µM Di-8-ANEPPQ at RT. After incubation, live images of cultured cells were taken using spinning disk confocal microscopy (Zeiss). The excitation and emission properties of the Di-8-ANEPPQ was obtained using the power spectrum module of confocal microscopy (LSM 780, Zeiss). The images of the in vivo heart operation were captured using the stereomicroscope (Discovery 8, Zeiss). The Di-8-ANEPPQ, in 1 µl containing 10 mg/ml, was injected into the apex of the heart. Following 2-3 hrs of the surgery, the heart was dissected out and the injection area was imaged using confocal microscopy (LSM 780, Zeiss). All images were analyzed and prepared using Fiji software..

Techniques: In Vitro, In Vivo, Microscopy, Software, Staining, Labeling, Injection, Incubation, Cell Culture, Confocal Microscopy