Structured Review

Thermo Fisher dh5αmcr
(A) Expression of the PvpA protein in E. coli under T7 promoter control. E. coli <t>DH5αMCR</t> cells harboring the recombinant plasmid pKPV.2 were subjected to selective induction of the T7 promoter. An SDS-PAGE autoradiograph of [ 35 S]methionine-labeled proteins expressed in E. coli (left panel) or a Western blot analysis of the same proteins as depicted in the left panel with MAb 1E5 (right panel) is shown. Cells in both panels were used without induction (lanes 1), with induction of the T7 promoter (lanes 2), or with induction in the presence of rifampin (lanes 3). A labeled arrow indicates the recombinant PvpA 55-kDa product expressed in E. coli . (B) Amino acid sequence and structural features of the PvpA protein from M. gallisepticum strain R. Amino acid residues are numbered on the right. A broken line marks the putative PvpA signal peptide. Labeled arrows show the position and direction of two directly repeated amino acid sequences (DR-1 and DR-2). A repeated motif consisting of four amino acids, PRPX (X is Met, Gln, or Asn), which appears 14 times within the PvpA C-terminal region is highlighted by boldface. The fourth codon, within a tract of five glutamine residues and in which a nonsense mutation has occurred, is marked by an arrowhead. Sixty proline residues within the C-terminal region are marked by asterisks.
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Images

1) Product Images from "Molecular Characterization of the Mycoplasma gallisepticum pvpA Gene Which Encodes a Putative Variable Cytadhesin Protein"

Article Title: Molecular Characterization of the Mycoplasma gallisepticum pvpA Gene Which Encodes a Putative Variable Cytadhesin Protein

Journal: Infection and Immunity

doi:

(A) Expression of the PvpA protein in E. coli under T7 promoter control. E. coli DH5αMCR cells harboring the recombinant plasmid pKPV.2 were subjected to selective induction of the T7 promoter. An SDS-PAGE autoradiograph of [ 35 S]methionine-labeled proteins expressed in E. coli (left panel) or a Western blot analysis of the same proteins as depicted in the left panel with MAb 1E5 (right panel) is shown. Cells in both panels were used without induction (lanes 1), with induction of the T7 promoter (lanes 2), or with induction in the presence of rifampin (lanes 3). A labeled arrow indicates the recombinant PvpA 55-kDa product expressed in E. coli . (B) Amino acid sequence and structural features of the PvpA protein from M. gallisepticum strain R. Amino acid residues are numbered on the right. A broken line marks the putative PvpA signal peptide. Labeled arrows show the position and direction of two directly repeated amino acid sequences (DR-1 and DR-2). A repeated motif consisting of four amino acids, PRPX (X is Met, Gln, or Asn), which appears 14 times within the PvpA C-terminal region is highlighted by boldface. The fourth codon, within a tract of five glutamine residues and in which a nonsense mutation has occurred, is marked by an arrowhead. Sixty proline residues within the C-terminal region are marked by asterisks.
Figure Legend Snippet: (A) Expression of the PvpA protein in E. coli under T7 promoter control. E. coli DH5αMCR cells harboring the recombinant plasmid pKPV.2 were subjected to selective induction of the T7 promoter. An SDS-PAGE autoradiograph of [ 35 S]methionine-labeled proteins expressed in E. coli (left panel) or a Western blot analysis of the same proteins as depicted in the left panel with MAb 1E5 (right panel) is shown. Cells in both panels were used without induction (lanes 1), with induction of the T7 promoter (lanes 2), or with induction in the presence of rifampin (lanes 3). A labeled arrow indicates the recombinant PvpA 55-kDa product expressed in E. coli . (B) Amino acid sequence and structural features of the PvpA protein from M. gallisepticum strain R. Amino acid residues are numbered on the right. A broken line marks the putative PvpA signal peptide. Labeled arrows show the position and direction of two directly repeated amino acid sequences (DR-1 and DR-2). A repeated motif consisting of four amino acids, PRPX (X is Met, Gln, or Asn), which appears 14 times within the PvpA C-terminal region is highlighted by boldface. The fourth codon, within a tract of five glutamine residues and in which a nonsense mutation has occurred, is marked by an arrowhead. Sixty proline residues within the C-terminal region are marked by asterisks.

Techniques Used: Expressing, Recombinant, Plasmid Preparation, SDS Page, Autoradiography, Labeling, Western Blot, Sequencing, Mutagenesis

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Gel Purification:

Article Title: Cytolethal Distending Toxin (CDT)-Negative Campylobacter jejuni Strains and Anti-CDT Neutralizing Antibodies Are Induced during Human Infection but Not during Colonization in Chickens
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