dh10b  (Thermo Fisher)


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    Name:
    ElectroMAX DH10B Cells
    Description:
    ElectroMAX DH10B Cells are electrocompetent E coli cells offering the highest transformation efficiencies of 1 x 1010 cfu µg plasmid DNA ElectroMAX DH10B Cells are ideal for applications requiring high transformation efficiencies such as with cDNA or gDNA library construction ElectroMAX DH10B Cells provide • High efficiency transformation to maximize clones• Enhanced genetic markers for cDNA or gDNA cloning capabilitiesHighest transformation efficiencies ElectroMAX DH10B Cells offer the highest transformation efficiencies available with 1 x 1010 transformants µg of control DNA in a 20 µL reaction These high transformation efficiencies make ElectroMAX DH10B Cells ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes ElectroMAX DH10B Cells are also suitable for construction of gene banks for generation of cDNA libraries using plasmid derived vectors and for situations when there is limited amount of DNA Note A high voltage electroporation apparatus capable of generating field strengths of 16 kV cm is required Versatile cloning capabilitiesElectroMAX DH10B Cells have the following features • Φ80lacZΔM15 marker provides α complementation of the β galactosidase gene allowing blue white screening on agar plates containing X gal or Bluo gal• mcrA genotypic marker and the mcrBC mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine• endA1 mutation enables increased plasmid yield and quantityGenotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ ara leu 7697 galU galK λ rpsL nupGFind the strain and format that you needDH10B cells are available in both electrocompetent and chemically competent formats Our ElectroMax DH10B T1R Cells and MegaX DH10B T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance
    Catalog Number:
    18290015
    Price:
    None
    Applications:
    Cloning|Electrocompetent Cells for Cloning|Transformation
    Category:
    Competent Cells Strains
    Buy from Supplier


    Structured Review

    Thermo Fisher dh10b
    Generation of a BAC of E1-deleted Ad3 encoding the eGFP reporter gene. (A) The plasmid pBSAd3LRzeo contains a zeocin selection cassette flanked by a 468-bp fragment of the Ad3 left end sequence and a 528-bp fragment of the Ad3 right end sequence. (B) Transfer of the Not IB– Hin dIII fragment containing the Ad3LRzeo cassette to the single copy pKSB2 plasmid. (C) Transformation of <t>DH10B</t> bacteria expressing the phage lambda recombinases allows homologous recombination between the Sal I-linearized pKSB2Ad3LRzeo and Ad3 genomic DNA, resulting in pKSBAd3wt. (D) Subsequent homologous recombination between pKSBAd3wt and a CMV-eGFP/zeo cassette flanked by short homologous sequences of 40 bp results in pKSB2 Ad3CMV-eGFP, which contains the eGFP expression cassette in reverse orientation. (E) Release of the viral genome by the flanking unique Mlu I endonuclease sites followed by transfection of helper 911-Ad3E1B cells yielding Ad3CMV-eGFP. (For details of the procedure, see Materials and methods .)
    ElectroMAX DH10B Cells are electrocompetent E coli cells offering the highest transformation efficiencies of 1 x 1010 cfu µg plasmid DNA ElectroMAX DH10B Cells are ideal for applications requiring high transformation efficiencies such as with cDNA or gDNA library construction ElectroMAX DH10B Cells provide • High efficiency transformation to maximize clones• Enhanced genetic markers for cDNA or gDNA cloning capabilitiesHighest transformation efficiencies ElectroMAX DH10B Cells offer the highest transformation efficiencies available with 1 x 1010 transformants µg of control DNA in a 20 µL reaction These high transformation efficiencies make ElectroMAX DH10B Cells ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes ElectroMAX DH10B Cells are also suitable for construction of gene banks for generation of cDNA libraries using plasmid derived vectors and for situations when there is limited amount of DNA Note A high voltage electroporation apparatus capable of generating field strengths of 16 kV cm is required Versatile cloning capabilitiesElectroMAX DH10B Cells have the following features • Φ80lacZΔM15 marker provides α complementation of the β galactosidase gene allowing blue white screening on agar plates containing X gal or Bluo gal• mcrA genotypic marker and the mcrBC mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine• endA1 mutation enables increased plasmid yield and quantityGenotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ ara leu 7697 galU galK λ rpsL nupGFind the strain and format that you needDH10B cells are available in both electrocompetent and chemically competent formats Our ElectroMax DH10B T1R Cells and MegaX DH10B T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance
    https://www.bioz.com/result/dh10b/product/Thermo Fisher
    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    dh10b - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "The nucleotide sequence and a first generation gene transfer vector of species B human adenovirus serotype 3"

    Article Title: The nucleotide sequence and a first generation gene transfer vector of species B human adenovirus serotype 3

    Journal: Virology

    doi: 10.1016/j.virol.2005.08.024

    Generation of a BAC of E1-deleted Ad3 encoding the eGFP reporter gene. (A) The plasmid pBSAd3LRzeo contains a zeocin selection cassette flanked by a 468-bp fragment of the Ad3 left end sequence and a 528-bp fragment of the Ad3 right end sequence. (B) Transfer of the Not IB– Hin dIII fragment containing the Ad3LRzeo cassette to the single copy pKSB2 plasmid. (C) Transformation of DH10B bacteria expressing the phage lambda recombinases allows homologous recombination between the Sal I-linearized pKSB2Ad3LRzeo and Ad3 genomic DNA, resulting in pKSBAd3wt. (D) Subsequent homologous recombination between pKSBAd3wt and a CMV-eGFP/zeo cassette flanked by short homologous sequences of 40 bp results in pKSB2 Ad3CMV-eGFP, which contains the eGFP expression cassette in reverse orientation. (E) Release of the viral genome by the flanking unique Mlu I endonuclease sites followed by transfection of helper 911-Ad3E1B cells yielding Ad3CMV-eGFP. (For details of the procedure, see Materials and methods .)
    Figure Legend Snippet: Generation of a BAC of E1-deleted Ad3 encoding the eGFP reporter gene. (A) The plasmid pBSAd3LRzeo contains a zeocin selection cassette flanked by a 468-bp fragment of the Ad3 left end sequence and a 528-bp fragment of the Ad3 right end sequence. (B) Transfer of the Not IB– Hin dIII fragment containing the Ad3LRzeo cassette to the single copy pKSB2 plasmid. (C) Transformation of DH10B bacteria expressing the phage lambda recombinases allows homologous recombination between the Sal I-linearized pKSB2Ad3LRzeo and Ad3 genomic DNA, resulting in pKSBAd3wt. (D) Subsequent homologous recombination between pKSBAd3wt and a CMV-eGFP/zeo cassette flanked by short homologous sequences of 40 bp results in pKSB2 Ad3CMV-eGFP, which contains the eGFP expression cassette in reverse orientation. (E) Release of the viral genome by the flanking unique Mlu I endonuclease sites followed by transfection of helper 911-Ad3E1B cells yielding Ad3CMV-eGFP. (For details of the procedure, see Materials and methods .)

    Techniques Used: BAC Assay, Plasmid Preparation, Selection, Sequencing, Transformation Assay, Expressing, Homologous Recombination, Transfection

    2) Product Images from "A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds"

    Article Title: A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds

    Journal: Micromachines

    doi: 10.3390/mi8080230

    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
    Figure Legend Snippet: Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.

    Techniques Used: Staining, Infection

    3) Product Images from "A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds"

    Article Title: A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds

    Journal: Micromachines

    doi: 10.3390/mi8080230

    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
    Figure Legend Snippet: Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.

    Techniques Used: Staining, Infection

    4) Product Images from "A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds"

    Article Title: A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds

    Journal: Micromachines

    doi: 10.3390/mi8080230

    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
    Figure Legend Snippet: Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.

    Techniques Used: Staining, Infection

    5) Product Images from "A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds"

    Article Title: A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds

    Journal: Micromachines

    doi: 10.3390/mi8080230

    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
    Figure Legend Snippet: Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.

    Techniques Used: Staining, Infection

    6) Product Images from "Construction and Characterization of an Infectious Murine Gammaherpesivrus-68 Bacterial Artificial Chromosome"

    Article Title: Construction and Characterization of an Infectious Murine Gammaherpesivrus-68 Bacterial Artificial Chromosome

    Journal: Journal of Biomedicine and Biotechnology

    doi: 10.1155/2011/926258

    The genomic structures of the MHV-68 recombinant viruses and the MHV-68 BAC plasmid. (a) Schematic diagram of the construction of the MHV-68 BAC virus. Shown is the left end of the viral genome encompassing terminal repeats and ORFs M1–M3. The BAC vector sequence and a puromycin expression cassette was recombined into the locus where the EGFP expression cassette was inserted in MHV-68(EGFP). MHV-68(BAC) was selected for the loss of the EGFP expression. The pMHV-68(BAC) plasmid was obtained by electroporating the circular genome of MHV-68(BAC) into DH10B cells. Following transfection of pMHV-68(BAC) into BHK-21 cells, the reconstituted virus, pMHV-68(BAC)v, was generated. The structures of pMHV-68(BAC) and pMHV-68(BAC)v are identical to MHV-68(BAC), except that pMHV-68(BAC) is circular with termini fused. To remove the BAC insert flanking by loxP sites, pMHV-68(BAC)v was co-transfected with a Cre recombinase expression plasmid and MHV-68(loxP) was isolated. The sites of Hind III are indicated with H and the ones of EcoR I were indicated with E. (b) Restriction enzyme analysis of the genome of MHV-68 recombinant viruses and the MHV-68 BAC plasmid. Each DNA sample was digested with either EcoR I or Hind III. Lane 1 is virion DNA of wild-type MHV-68; lane 2 is virion DNA of MHV-68(BAC); lane 3 is the plasmid DNA of pMHV-68(BAC); lane 4 is virion DNA of pMHV-68(BAC)v; lane 5 is virion DNA of MHV-68(loxP) and lane 6 is virion DNA of MHV-68(EGFP). The wild-type fragments which were eliminated due to the insertion of the BAC sequence were marked with asterisks. The fragments marked with a, b, d, and e, were generated because of the BAC insert. The fragments marked with c and f, resulted from the removal of BAC, 200 bp larger than the fragments indicated with asterisks. The fragments marked with F were from the fused termini of the circular pMHV-68(BAC). On the bottom of each agarose gel picture is the corresponding Southern blot using the probe spanning nt 51–6298. The laddering patterns in EcoR I-digested samples were due to various number of repeats on the linear virion DNA but not present in pMHV-68(BAC) (lane 3).
    Figure Legend Snippet: The genomic structures of the MHV-68 recombinant viruses and the MHV-68 BAC plasmid. (a) Schematic diagram of the construction of the MHV-68 BAC virus. Shown is the left end of the viral genome encompassing terminal repeats and ORFs M1–M3. The BAC vector sequence and a puromycin expression cassette was recombined into the locus where the EGFP expression cassette was inserted in MHV-68(EGFP). MHV-68(BAC) was selected for the loss of the EGFP expression. The pMHV-68(BAC) plasmid was obtained by electroporating the circular genome of MHV-68(BAC) into DH10B cells. Following transfection of pMHV-68(BAC) into BHK-21 cells, the reconstituted virus, pMHV-68(BAC)v, was generated. The structures of pMHV-68(BAC) and pMHV-68(BAC)v are identical to MHV-68(BAC), except that pMHV-68(BAC) is circular with termini fused. To remove the BAC insert flanking by loxP sites, pMHV-68(BAC)v was co-transfected with a Cre recombinase expression plasmid and MHV-68(loxP) was isolated. The sites of Hind III are indicated with H and the ones of EcoR I were indicated with E. (b) Restriction enzyme analysis of the genome of MHV-68 recombinant viruses and the MHV-68 BAC plasmid. Each DNA sample was digested with either EcoR I or Hind III. Lane 1 is virion DNA of wild-type MHV-68; lane 2 is virion DNA of MHV-68(BAC); lane 3 is the plasmid DNA of pMHV-68(BAC); lane 4 is virion DNA of pMHV-68(BAC)v; lane 5 is virion DNA of MHV-68(loxP) and lane 6 is virion DNA of MHV-68(EGFP). The wild-type fragments which were eliminated due to the insertion of the BAC sequence were marked with asterisks. The fragments marked with a, b, d, and e, were generated because of the BAC insert. The fragments marked with c and f, resulted from the removal of BAC, 200 bp larger than the fragments indicated with asterisks. The fragments marked with F were from the fused termini of the circular pMHV-68(BAC). On the bottom of each agarose gel picture is the corresponding Southern blot using the probe spanning nt 51–6298. The laddering patterns in EcoR I-digested samples were due to various number of repeats on the linear virion DNA but not present in pMHV-68(BAC) (lane 3).

    Techniques Used: Recombinant, BAC Assay, Plasmid Preparation, Sequencing, Expressing, Transfection, Generated, Isolation, Agarose Gel Electrophoresis, Southern Blot

    7) Product Images from "A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds"

    Article Title: A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds

    Journal: Micromachines

    doi: 10.3390/mi8080230

    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
    Figure Legend Snippet: Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.

    Techniques Used: Staining, Infection

    8) Product Images from "Development of a Native Escherichia coli Induction System for Ionic Liquid Tolerance"

    Article Title: Development of a Native Escherichia coli Induction System for Ionic Liquid Tolerance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0101115

    Growth of different eilA expression strains at increasing [C 2 mim]Cl concentrations. A–E: growth assays of E. coli DH10B carrying different promoter- eilA constructs. Due to day-to-day variability in the final cell density reached in the microtiter-plate experiments, growth curves were normalized to a start OD of 0 and a maximum OD of 0.5. A) pP ydfO’ - eilA , B) pP ydfA’-eilA , C) pP marR’-eilA , D) pP lacUV5-eilA, E) pP- eilA . Error bars represent standard errors. Blue: 0 mM [C 2 mim]Cl, red: 100 mM [C 2 mim]Cl, green: 200 mM [C 2 mim]Cl, purple: 400 mM [C 2 mim]Cl.
    Figure Legend Snippet: Growth of different eilA expression strains at increasing [C 2 mim]Cl concentrations. A–E: growth assays of E. coli DH10B carrying different promoter- eilA constructs. Due to day-to-day variability in the final cell density reached in the microtiter-plate experiments, growth curves were normalized to a start OD of 0 and a maximum OD of 0.5. A) pP ydfO’ - eilA , B) pP ydfA’-eilA , C) pP marR’-eilA , D) pP lacUV5-eilA, E) pP- eilA . Error bars represent standard errors. Blue: 0 mM [C 2 mim]Cl, red: 100 mM [C 2 mim]Cl, green: 200 mM [C 2 mim]Cl, purple: 400 mM [C 2 mim]Cl.

    Techniques Used: Expressing, Construct

    9) Product Images from "The DNA strand of chimeric RNA/DNA oligonucleotides can direct gene repair/conversion activity in mammalian and plant cell-free extracts"

    Article Title: The DNA strand of chimeric RNA/DNA oligonucleotides can direct gene repair/conversion activity in mammalian and plant cell-free extracts

    Journal: Nucleic Acids Research

    doi:

    Genetic readout system for correction of a point mutation in plasmid pK s m4021. A mutant kanamycin gene harbored in plasmid pK s m4021 is the target for correction by oligonucleotides. The mutant G is converted to a C by the action of the oligo. Corrected plasmids confer resistance to kanamycin in E.coli strain DH10B after electroporation leading to the genetic readout and colony counts.
    Figure Legend Snippet: Genetic readout system for correction of a point mutation in plasmid pK s m4021. A mutant kanamycin gene harbored in plasmid pK s m4021 is the target for correction by oligonucleotides. The mutant G is converted to a C by the action of the oligo. Corrected plasmids confer resistance to kanamycin in E.coli strain DH10B after electroporation leading to the genetic readout and colony counts.

    Techniques Used: Mutagenesis, Plasmid Preparation, Electroporation

    10) Product Images from "Biochemical Characterization of Pathogenic Mutations in Human Mitochondrial Methionyl-tRNA Formyltransferase *"

    Article Title: Biochemical Characterization of Pathogenic Mutations in Human Mitochondrial Methionyl-tRNA Formyltransferase *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.610626

    Analysis of the effect of mutations in MTF s in vivo . A and B , growth of E. coli DH10B co-transformants, containing pRSVCATam 1.2.5 and one of the pACD MTF s constructs, on 2× YT agar plates containing 50 μg/ml chloramphenicol. The growth
    Figure Legend Snippet: Analysis of the effect of mutations in MTF s in vivo . A and B , growth of E. coli DH10B co-transformants, containing pRSVCATam 1.2.5 and one of the pACD MTF s constructs, on 2× YT agar plates containing 50 μg/ml chloramphenicol. The growth

    Techniques Used: In Vivo, Construct

    Related Articles

    Electroporation:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: .. Plasmid DNA was transformed into ElectroMAX DH10B cells through electroporation (Invitrogen, USA) by mixing 5μl of isolated plasmid DNA with 20μl of electro-competent cells. .. Electroporation conditions were 25 μF, 200 Ω, and 2.0 Kv.

    Article Title: Engineered biosensors from dimeric ligand-binding domains
    Article Snippet: .. The plasmid library was transformed into ElectroMAX DH10B cells (Thermo Fisher Scientific, Waltham, MA) via electroporation per manufacturer’s protocol. ..

    Clone Assay:

    Article Title: Isolation and Characterization of cDNAs Expressed in the Early Stages of Flavonol-Induced Pollen Germination in Petunia 1
    Article Snippet: .. The [−Fl] cDNA was ligated into pSport2 and electroporated into Electromax DH12S Escherichia coli (Gibco-BRL), whereas the [+Fl] cDNA was cloned into pSport1 and transformed into Electromax DH10B cells (Gibco-BRL). ..

    Article Title: Epitope-Based Vaccines with the Anaplasma marginale MSP1a Functional Motif Induce a Balanced Humoral and Cellular Immune Response in Mice
    Article Snippet: .. Standards curves were constructed by cloning PCR products of MSP5 gene fragments using TOPO TA Cloning Dual Promoter Kit (Life Technologies), and then transformed into chemically competent E.coli DH10B competent cells (ElectroMAX DH10B™ Cells - Life Technologies). .. Plasmids were extracted using a QIAprep spin miniprep kit (Qiagen) and sequenced using MegaBACE 1000 automatic sequencer (Molecular Dynamics).

    In Vitro:

    Article Title: Cloning and Characterization of RGS9-2: A Striatal-Enriched Alternatively Spliced Product of the RGS9 Gene
    Article Snippet: .. Briefly, transposons were randomly integrated into the target DNA in an in vitro transposition reaction; the DNA was isopropanol-precipitated and electroporated into competent cells (ElectroMax DH10B, Life Technologies, Rockville, MD). .. Double antibiotic selection was used to isolate transformants having the genomic insert and the incorporated transposon.

    Ligation:

    Article Title: Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production
    Article Snippet: .. Ligation reactions were transformed into ElectroMAX™ DH10B™ E. coli cells (Invitrogen), obtaining > 100,000 cfu for each population. .. Plasmid libraries harvested from these cells were then introduced into genomically recoded C321.ΔA.ΔserB E. coli including either a plasmid containing the serine tRNA amber suppressor supD or SepOTSλ, allowing Ser or phosphoserine incorporation at amber codons, respectively. ( ; )

    Isolation:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: .. Plasmid DNA was transformed into ElectroMAX DH10B cells through electroporation (Invitrogen, USA) by mixing 5μl of isolated plasmid DNA with 20μl of electro-competent cells. .. Electroporation conditions were 25 μF, 200 Ω, and 2.0 Kv.

    TA Cloning:

    Article Title: Epitope-Based Vaccines with the Anaplasma marginale MSP1a Functional Motif Induce a Balanced Humoral and Cellular Immune Response in Mice
    Article Snippet: .. Standards curves were constructed by cloning PCR products of MSP5 gene fragments using TOPO TA Cloning Dual Promoter Kit (Life Technologies), and then transformed into chemically competent E.coli DH10B competent cells (ElectroMAX DH10B™ Cells - Life Technologies). .. Plasmids were extracted using a QIAprep spin miniprep kit (Qiagen) and sequenced using MegaBACE 1000 automatic sequencer (Molecular Dynamics).

    Construct:

    Article Title: Epitope-Based Vaccines with the Anaplasma marginale MSP1a Functional Motif Induce a Balanced Humoral and Cellular Immune Response in Mice
    Article Snippet: .. Standards curves were constructed by cloning PCR products of MSP5 gene fragments using TOPO TA Cloning Dual Promoter Kit (Life Technologies), and then transformed into chemically competent E.coli DH10B competent cells (ElectroMAX DH10B™ Cells - Life Technologies). .. Plasmids were extracted using a QIAprep spin miniprep kit (Qiagen) and sequenced using MegaBACE 1000 automatic sequencer (Molecular Dynamics).

    Polymerase Chain Reaction:

    Article Title: Characterization of blaCMY-2 Plasmids in Salmonella and Escherichia coli Isolates from Food Animals in Canada
    Article Snippet: .. Eight pairs of cefoxitin-resistant S. enterica and E. coli isolates from the same chicken ceca were included. bla CMY was detected by PCR ( , ), and plasmid DNA (Qiagen plasmid kit; Qiagen, Hilden, Germany) was electroporated into E. coli ElectroMAX DH10B (Invitrogen, Carlsbad, CA). .. Transformants were selected on Mueller-Hinton agar (BD, Franklin Lakes, NJ) containing 8 mg/liter ceftiofur (Sigma-Aldrich, St. Louis, MO).

    Article Title: Epitope-Based Vaccines with the Anaplasma marginale MSP1a Functional Motif Induce a Balanced Humoral and Cellular Immune Response in Mice
    Article Snippet: .. Standards curves were constructed by cloning PCR products of MSP5 gene fragments using TOPO TA Cloning Dual Promoter Kit (Life Technologies), and then transformed into chemically competent E.coli DH10B competent cells (ElectroMAX DH10B™ Cells - Life Technologies). .. Plasmids were extracted using a QIAprep spin miniprep kit (Qiagen) and sequenced using MegaBACE 1000 automatic sequencer (Molecular Dynamics).

    Transformation Assay:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: .. Plasmid DNA was transformed into ElectroMAX DH10B cells through electroporation (Invitrogen, USA) by mixing 5μl of isolated plasmid DNA with 20μl of electro-competent cells. .. Electroporation conditions were 25 μF, 200 Ω, and 2.0 Kv.

    Article Title: Isolation and Characterization of cDNAs Expressed in the Early Stages of Flavonol-Induced Pollen Germination in Petunia 1
    Article Snippet: .. The [−Fl] cDNA was ligated into pSport2 and electroporated into Electromax DH12S Escherichia coli (Gibco-BRL), whereas the [+Fl] cDNA was cloned into pSport1 and transformed into Electromax DH10B cells (Gibco-BRL). ..

    Article Title: Epitope-Based Vaccines with the Anaplasma marginale MSP1a Functional Motif Induce a Balanced Humoral and Cellular Immune Response in Mice
    Article Snippet: .. Standards curves were constructed by cloning PCR products of MSP5 gene fragments using TOPO TA Cloning Dual Promoter Kit (Life Technologies), and then transformed into chemically competent E.coli DH10B competent cells (ElectroMAX DH10B™ Cells - Life Technologies). .. Plasmids were extracted using a QIAprep spin miniprep kit (Qiagen) and sequenced using MegaBACE 1000 automatic sequencer (Molecular Dynamics).

    Article Title: Engineered biosensors from dimeric ligand-binding domains
    Article Snippet: .. The plasmid library was transformed into ElectroMAX DH10B cells (Thermo Fisher Scientific, Waltham, MA) via electroporation per manufacturer’s protocol. ..

    Article Title: Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production
    Article Snippet: .. Ligation reactions were transformed into ElectroMAX™ DH10B™ E. coli cells (Invitrogen), obtaining > 100,000 cfu for each population. .. Plasmid libraries harvested from these cells were then introduced into genomically recoded C321.ΔA.ΔserB E. coli including either a plasmid containing the serine tRNA amber suppressor supD or SepOTSλ, allowing Ser or phosphoserine incorporation at amber codons, respectively. ( ; )

    Plasmid Preparation:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: .. Plasmid DNA was transformed into ElectroMAX DH10B cells through electroporation (Invitrogen, USA) by mixing 5μl of isolated plasmid DNA with 20μl of electro-competent cells. .. Electroporation conditions were 25 μF, 200 Ω, and 2.0 Kv.

    Article Title: Characterization of blaCMY-2 Plasmids in Salmonella and Escherichia coli Isolates from Food Animals in Canada
    Article Snippet: .. Eight pairs of cefoxitin-resistant S. enterica and E. coli isolates from the same chicken ceca were included. bla CMY was detected by PCR ( , ), and plasmid DNA (Qiagen plasmid kit; Qiagen, Hilden, Germany) was electroporated into E. coli ElectroMAX DH10B (Invitrogen, Carlsbad, CA). .. Transformants were selected on Mueller-Hinton agar (BD, Franklin Lakes, NJ) containing 8 mg/liter ceftiofur (Sigma-Aldrich, St. Louis, MO).

    Article Title: Engineered biosensors from dimeric ligand-binding domains
    Article Snippet: .. The plasmid library was transformed into ElectroMAX DH10B cells (Thermo Fisher Scientific, Waltham, MA) via electroporation per manufacturer’s protocol. ..

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    Thermo Fisher dh10b
    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive <t>DH10B</t> showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.
    Dh10b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dh10b - by Bioz Stars, 2020-09
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    93
    Thermo Fisher e coli dh10b
    SBD-homologous domains might function to recognize PT-DNA. a Multiple sequence alignment of representative ScoMcrA-SBD homologs from Streptomyces coelicolor (#1), Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4). The sulfur-recognizing residues are highlighted in yellow. The consensus sequence is shown at the bottom. Right: Domain organizations of these ScoMcrA homologs. b The surface of ScoMcrA-SBD is colored according to conservation scores, which shows that its sulfur-recognizing cavity is highly conserved. c Purified ScoMcrA-SBD domain homologs from Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4) specifically recognized PT-DNA with the sulfur atom in the R p , but not in the S p , configuration. d Mutation of P482N in the Escherichia coli SBD homolog significantly diminished, while mutation of P607N in the Streptomyces gancidicus SBD homolog disrupted, the association with PT-DNA with the core sequence G PS AAC. e Heterologous expression of scomcrA homologs from S. gancidicus (#2), E. coli (#3), and M. morganii (#4) restricted transfer of the dnd gene cluster from Salmonella enterica , which contains the genes encoding the “writer” proteins of DNA phosphorothioation. Transformation frequencies of empty pBluescript vector (PT − ) and that harboring the dnd gene cluster (PT + ) into E. coli <t>DH10B</t> expressing various scomcrA homologs are shown
    E Coli Dh10b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 68 article reviews
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    Image Search Results


    Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.

    Journal: Micromachines

    Article Title: A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds

    doi: 10.3390/mi8080230

    Figure Lengend Snippet: Annexin V staining demonstrates the apoptotic effect of bacterially delivered violacein at a MOI of 25 at 10 h post-infection. ( a ) 7% of HeLa S3 cells treated with a control invasive DH10B showed annexin V staining (right panel); ( b ) 33% of HeLa S3 cells treated with bacterially delivered violacein showed annexin V staining. Scale bars represent 100 μm.

    Article Snippet: We dosed a suspension of HeLa S3 cells with violacein producing invasive DH10B in droplets to confirm if a drug producing operon could be detected and at least partially enriched above background in one cycle of cell sorting.

    Techniques: Staining, Infection

    SBD-homologous domains might function to recognize PT-DNA. a Multiple sequence alignment of representative ScoMcrA-SBD homologs from Streptomyces coelicolor (#1), Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4). The sulfur-recognizing residues are highlighted in yellow. The consensus sequence is shown at the bottom. Right: Domain organizations of these ScoMcrA homologs. b The surface of ScoMcrA-SBD is colored according to conservation scores, which shows that its sulfur-recognizing cavity is highly conserved. c Purified ScoMcrA-SBD domain homologs from Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4) specifically recognized PT-DNA with the sulfur atom in the R p , but not in the S p , configuration. d Mutation of P482N in the Escherichia coli SBD homolog significantly diminished, while mutation of P607N in the Streptomyces gancidicus SBD homolog disrupted, the association with PT-DNA with the core sequence G PS AAC. e Heterologous expression of scomcrA homologs from S. gancidicus (#2), E. coli (#3), and M. morganii (#4) restricted transfer of the dnd gene cluster from Salmonella enterica , which contains the genes encoding the “writer” proteins of DNA phosphorothioation. Transformation frequencies of empty pBluescript vector (PT − ) and that harboring the dnd gene cluster (PT + ) into E. coli DH10B expressing various scomcrA homologs are shown

    Journal: Nature Communications

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA

    doi: 10.1038/s41467-018-07093-1

    Figure Lengend Snippet: SBD-homologous domains might function to recognize PT-DNA. a Multiple sequence alignment of representative ScoMcrA-SBD homologs from Streptomyces coelicolor (#1), Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4). The sulfur-recognizing residues are highlighted in yellow. The consensus sequence is shown at the bottom. Right: Domain organizations of these ScoMcrA homologs. b The surface of ScoMcrA-SBD is colored according to conservation scores, which shows that its sulfur-recognizing cavity is highly conserved. c Purified ScoMcrA-SBD domain homologs from Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4) specifically recognized PT-DNA with the sulfur atom in the R p , but not in the S p , configuration. d Mutation of P482N in the Escherichia coli SBD homolog significantly diminished, while mutation of P607N in the Streptomyces gancidicus SBD homolog disrupted, the association with PT-DNA with the core sequence G PS AAC. e Heterologous expression of scomcrA homologs from S. gancidicus (#2), E. coli (#3), and M. morganii (#4) restricted transfer of the dnd gene cluster from Salmonella enterica , which contains the genes encoding the “writer” proteins of DNA phosphorothioation. Transformation frequencies of empty pBluescript vector (PT − ) and that harboring the dnd gene cluster (PT + ) into E. coli DH10B expressing various scomcrA homologs are shown

    Article Snippet: The pACYCDuet™-1 vector and its derivatives carrying FL E. coli , M. morganii , and S. gancidicus ScoMcrA homologs with E. coli promoters (pJTU1673, pJTU1674, and pJTU1675) were introduced to E. coli DH10B, and competent cells of the resulting strains were prepared using the standard calcium chloride protocol.

    Techniques: Sequencing, Purification, Mutagenesis, Expressing, Transformation Assay, Plasmid Preparation