dextran sulfate m r 8000  (Millipore)


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    Name:
    D Ribulose 1 5 Diphosphate Carboxylase from spinach
    Description:
    Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa Each molecule contains one magnesium ion pH optimum 7 9 KM for CO2 0 45 mM Ribulose diphosphate becomes inhibitory at concentrations exceeding 0 7 mM Orthophosphate and ammonium sulfate are competitive inhibitors 3 Phosphoglycerate is a noncompetitive inhibitor
    Catalog Number:
    r8000
    Price:
    None
    Applications:
    D-Ribulose 1,5-diphosphate carboxylase from spinach has been used in a study to assess catalytic by-product formation and ligand binding by ribulose bisphosphate carboxylases from different phylogenies. D-Ribulose 1,5-diphosphate carboxylase from spinach has also been used in a study to determine RuBisCO activation kinetics and other rate and equilibrium constants by simultaneous multiple non-linear regression of a kinetic model.
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    Structured Review

    Millipore dextran sulfate m r 8000
    Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa Each molecule contains one magnesium ion pH optimum 7 9 KM for CO2 0 45 mM Ribulose diphosphate becomes inhibitory at concentrations exceeding 0 7 mM Orthophosphate and ammonium sulfate are competitive inhibitors 3 Phosphoglycerate is a noncompetitive inhibitor
    https://www.bioz.com/result/dextran sulfate m r 8000/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dextran sulfate m r 8000 - by Bioz Stars, 2020-09
    91/100 stars

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    Related Articles

    Isothermal Titration Calorimetry:

    Article Title: Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
    Article Snippet: .. Isothermal titration calorimetry The same, commercially obtained spinach Rubisco (Sigma-Aldrich; catalogue # R8000; ; specific activity 0.05 units / mg) was inactivated by dissolving in 10 mM HEPES buffer pH 7.9 containing 100 μM EDTA and dialyzing twice against 2 L of ITC buffer (10 mM HEPES buffer pH 7.9) using a 3,500 MWCO dialysis membrane. .. A low volume Nano ITC instrument (TA Instruments) with a cell volume of 190 μL was used for this study.

    Purification:

    Article Title: Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
    Article Snippet: .. Radiolabelled [3 H]-(±)-ABA binding assays An SDS-PAGE analysis of commercially available partially purified spinach Rubisco (Sigma-Aldrich; Catalogue # R8000; specific activity 0.05 units / mg) shows the presence of two, otherwise pure bands that correlate with the expected molecular weights of large (55 kDa) and small (13 kDa) solubilized Rubisco subunits, confirming its purity is sufficient for analysis ( ). .. A fresh aliquot of this Rubisco was subsequently dissolved in binding buffer (50 mM bicine, 15 mM MgCl2 , 20 mM NaCl, pH 7.9) and placed on ice.

    Article Title: Analyzing pepsin degradation assay conditions used for allergenicity assessments to ensure that pepsin susceptible and pepsin resistant dietary proteins are distinguishable
    Article Snippet: .. Enzymes and substrate proteins Purified porcine pepsin (Cat. #P6887), ribulose 1,5-diphosphate carboxylase (Rubisco) (Cat. #R8000), hemoglobin (Hb) (Cat. #H2625), and soybean trypsin inhibitor (STI) (Cat. #T9003) were obtained from Sigma Aldrich (St Louis, MO). .. Horseradish peroxidase (HRP) (Cat. #31490) was purchased from Thermo Scientific (Grand Island, NY).

    Article Title: Discovery of chemoautotrophic symbiosis in the giant shipworm Kuphus polythalamia (Bivalvia: Teredinidae) extends wooden-steps theory
    Article Snippet: .. Positive controls contained purified spinach RuBisCO (Sigma; R8000-1UN). ..

    Activation Assay:

    Article Title: Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
    Article Snippet: .. Rubisco activity assays Commercially available Rubisco from spinach (Sigma-Aldrich; catalogue # R8000; ; specific activity 0.05 units / mg) was dissolved in a buffer containing 15 mM MgCl2 and 25 mM NaHCO3 at pH 8.0 for activation. ..

    Activity Assay:

    Article Title: Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
    Article Snippet: .. Radiolabelled [3 H]-(±)-ABA binding assays An SDS-PAGE analysis of commercially available partially purified spinach Rubisco (Sigma-Aldrich; Catalogue # R8000; specific activity 0.05 units / mg) shows the presence of two, otherwise pure bands that correlate with the expected molecular weights of large (55 kDa) and small (13 kDa) solubilized Rubisco subunits, confirming its purity is sufficient for analysis ( ). .. A fresh aliquot of this Rubisco was subsequently dissolved in binding buffer (50 mM bicine, 15 mM MgCl2 , 20 mM NaCl, pH 7.9) and placed on ice.

    Article Title: Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
    Article Snippet: .. Rubisco activity assays Commercially available Rubisco from spinach (Sigma-Aldrich; catalogue # R8000; ; specific activity 0.05 units / mg) was dissolved in a buffer containing 15 mM MgCl2 and 25 mM NaHCO3 at pH 8.0 for activation. ..

    Article Title: Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
    Article Snippet: .. Isothermal titration calorimetry The same, commercially obtained spinach Rubisco (Sigma-Aldrich; catalogue # R8000; ; specific activity 0.05 units / mg) was inactivated by dissolving in 10 mM HEPES buffer pH 7.9 containing 100 μM EDTA and dialyzing twice against 2 L of ITC buffer (10 mM HEPES buffer pH 7.9) using a 3,500 MWCO dialysis membrane. .. A low volume Nano ITC instrument (TA Instruments) with a cell volume of 190 μL was used for this study.

    Binding Assay:

    Article Title: Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
    Article Snippet: .. Radiolabelled [3 H]-(±)-ABA binding assays An SDS-PAGE analysis of commercially available partially purified spinach Rubisco (Sigma-Aldrich; Catalogue # R8000; specific activity 0.05 units / mg) shows the presence of two, otherwise pure bands that correlate with the expected molecular weights of large (55 kDa) and small (13 kDa) solubilized Rubisco subunits, confirming its purity is sufficient for analysis ( ). .. A fresh aliquot of this Rubisco was subsequently dissolved in binding buffer (50 mM bicine, 15 mM MgCl2 , 20 mM NaCl, pH 7.9) and placed on ice.

    SDS Page:

    Article Title: Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
    Article Snippet: .. Radiolabelled [3 H]-(±)-ABA binding assays An SDS-PAGE analysis of commercially available partially purified spinach Rubisco (Sigma-Aldrich; Catalogue # R8000; specific activity 0.05 units / mg) shows the presence of two, otherwise pure bands that correlate with the expected molecular weights of large (55 kDa) and small (13 kDa) solubilized Rubisco subunits, confirming its purity is sufficient for analysis ( ). .. A fresh aliquot of this Rubisco was subsequently dissolved in binding buffer (50 mM bicine, 15 mM MgCl2 , 20 mM NaCl, pH 7.9) and placed on ice.

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  • 99
    Millipore dextran sulfate sodium salt
    Dextran Sulfate Sodium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dextran sulfate sodium salt/product/Millipore
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dextran sulfate sodium salt - by Bioz Stars, 2020-09
    99/100 stars
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