Journal: Frontiers in Neuroscience
Article Title: Sulfate reducing bacteria induce α-synuclein in intestinal and neuronal cells and tissues and inhibit tyrosine hydroxylase in neuronal cells
doi: 10.3389/fnins.2025.1672793
Figure Lengend Snippet: Desulfovibrio inhibits tyrosine hydroxylase (TH) protein expression in neuronal Sy5y cells (A,B) . Cells were infected for 24 with DSV at various MOIs. Cells were harvested for western blotting to probe for TH expression. Actin was used as a loading control. Data represent mean ± SEM as a fold change difference compared to control. (C,D) Cells were infected for 24 with D. piger at various MOIs. Cells were harvested for western blotting to probe for TH expression. Western blot images were quantified using Fiji Image J. Data represent mean ± SEM as a fold change difference compared to control. Control values were set as 1. A two-tailed student t-test was used to analyze the statistical difference between control and DSV-infected cells. *** p < 0.001, ** p < 0.01, * p < 0.05.
Article Snippet: Desulfovibrio vulgaris Hildenborough (ATCC 29579, Manassas, VA) was grown anaerobically in Hungate tubes using Postgate’s organic liquid medium with the following composition: 10.56 mM Na 2 SO 4 , 13.29 mM MgSO 4 , 4.12 mM L-Cysteine, 0.4% sodium lactate (60% syrup), 0.4% yeast extract, and 0.5% tryptone.
Techniques: Expressing, Infection, Western Blot, Control, Two Tailed Test