destination vector gateway adapted pegfp c1 pdonr221 eps8  (Thermo Fisher)


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    Structured Review

    Thermo Fisher destination vector gateway adapted pegfp c1 pdonr221 eps8
    Destination Vector Gateway Adapted Pegfp C1 Pdonr221 Eps8, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/destination vector gateway adapted pegfp c1 pdonr221 eps8/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    destination vector gateway adapted pegfp c1 pdonr221 eps8 - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: IRTKS (BAIAP2L1) elongates epithelial microvilli using EPS8-dependent and independent mechanisms
    Article Snippet: Paragraph title: Cloning and Constructs: ... All entry vectors were verified by DNA sequencing and then shuttled into the destination vector Gateway-adapted pEGFP-C1—pDONR221 EPS8 (Open Biosystems) was also purchased and shuttled into Gateway-adapted pEGFP-C1 to generate pEGFP-C1-EPS8 (aa 1–822) and verified by sequencing.

    Mutagenesis:

    Article Title: IRTKS (BAIAP2L1) elongates epithelial microvilli using EPS8-dependent and independent mechanisms
    Article Snippet: To generate the IRTKS-SH3* construct, mutations W378K and W391K were introduced into pEGFP-IRTKS using site-directed mutagenesis. .. All entry vectors were verified by DNA sequencing and then shuttled into the destination vector Gateway-adapted pEGFP-C1—pDONR221 EPS8 (Open Biosystems) was also purchased and shuttled into Gateway-adapted pEGFP-C1 to generate pEGFP-C1-EPS8 (aa 1–822) and verified by sequencing.

    Live Cell Imaging:

    Article Title: IRTKS (BAIAP2L1) elongates epithelial microvilli using EPS8-dependent and independent mechanisms
    Article Snippet: All entry vectors were verified by DNA sequencing and then shuttled into the destination vector Gateway-adapted pEGFP-C1—pDONR221 EPS8 (Open Biosystems) was also purchased and shuttled into Gateway-adapted pEGFP-C1 to generate pEGFP-C1-EPS8 (aa 1–822) and verified by sequencing. .. The mCherry-tagged UtrCH used for B16F1, HeLa, and W4 live-cell imaging was purchased from Addgene (26740, deposited by W. Bement).

    Construct:

    Article Title: IRTKS (BAIAP2L1) elongates epithelial microvilli using EPS8-dependent and independent mechanisms
    Article Snippet: Paragraph title: Cloning and Constructs: ... All entry vectors were verified by DNA sequencing and then shuttled into the destination vector Gateway-adapted pEGFP-C1—pDONR221 EPS8 (Open Biosystems) was also purchased and shuttled into Gateway-adapted pEGFP-C1 to generate pEGFP-C1-EPS8 (aa 1–822) and verified by sequencing.

    Sequencing:

    Article Title: IRTKS (BAIAP2L1) elongates epithelial microvilli using EPS8-dependent and independent mechanisms
    Article Snippet: .. All entry vectors were verified by DNA sequencing and then shuttled into the destination vector Gateway-adapted pEGFP-C1—pDONR221 EPS8 (Open Biosystems) was also purchased and shuttled into Gateway-adapted pEGFP-C1 to generate pEGFP-C1-EPS8 (aa 1–822) and verified by sequencing. .. EPS8ΔPR1 (deleted aa 198–231) construct was generated by reverse PCR and TOPO cloned into the pCR8 Gateway entry vector.

    Generated:

    Article Title: IRTKS (BAIAP2L1) elongates epithelial microvilli using EPS8-dependent and independent mechanisms
    Article Snippet: IRTKSΔWH2 (aa 1–482), I-BAR alone (aa 1–249), ΔI-BAR (aa 250–511), WH2 alone (aa 473–511) constructs were generated by PCR and TOPO cloned into the pCR8 Gateway entry vector (Invitrogen). .. All entry vectors were verified by DNA sequencing and then shuttled into the destination vector Gateway-adapted pEGFP-C1—pDONR221 EPS8 (Open Biosystems) was also purchased and shuttled into Gateway-adapted pEGFP-C1 to generate pEGFP-C1-EPS8 (aa 1–822) and verified by sequencing.

    DNA Sequencing:

    Article Title: IRTKS (BAIAP2L1) elongates epithelial microvilli using EPS8-dependent and independent mechanisms
    Article Snippet: .. All entry vectors were verified by DNA sequencing and then shuttled into the destination vector Gateway-adapted pEGFP-C1—pDONR221 EPS8 (Open Biosystems) was also purchased and shuttled into Gateway-adapted pEGFP-C1 to generate pEGFP-C1-EPS8 (aa 1–822) and verified by sequencing. .. EPS8ΔPR1 (deleted aa 198–231) construct was generated by reverse PCR and TOPO cloned into the pCR8 Gateway entry vector.

    Polymerase Chain Reaction:

    Article Title: IRTKS (BAIAP2L1) elongates epithelial microvilli using EPS8-dependent and independent mechanisms
    Article Snippet: IRTKSΔWH2 (aa 1–482), I-BAR alone (aa 1–249), ΔI-BAR (aa 250–511), WH2 alone (aa 473–511) constructs were generated by PCR and TOPO cloned into the pCR8 Gateway entry vector (Invitrogen). .. All entry vectors were verified by DNA sequencing and then shuttled into the destination vector Gateway-adapted pEGFP-C1—pDONR221 EPS8 (Open Biosystems) was also purchased and shuttled into Gateway-adapted pEGFP-C1 to generate pEGFP-C1-EPS8 (aa 1–822) and verified by sequencing.

    Plasmid Preparation:

    Article Title: IRTKS (BAIAP2L1) elongates epithelial microvilli using EPS8-dependent and independent mechanisms
    Article Snippet: .. All entry vectors were verified by DNA sequencing and then shuttled into the destination vector Gateway-adapted pEGFP-C1—pDONR221 EPS8 (Open Biosystems) was also purchased and shuttled into Gateway-adapted pEGFP-C1 to generate pEGFP-C1-EPS8 (aa 1–822) and verified by sequencing. .. EPS8ΔPR1 (deleted aa 198–231) construct was generated by reverse PCR and TOPO cloned into the pCR8 Gateway entry vector.