PromoCell
human dermal microvascular endothelial cells hdmecs ![]() Human Dermal Microvascular Endothelial Cells Hdmecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/human dermal microvascular endothelial cells hdmecs/product/PromoCell Average 86 stars, based on 1 article reviews
human dermal microvascular endothelial cells hdmecs - by Bioz Stars,
2025-02
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Lonza
human dermal microvascular endothelial cells ![]() Human Dermal Microvascular Endothelial Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/human dermal microvascular endothelial cells/product/Lonza Average 86 stars, based on 1 article reviews
human dermal microvascular endothelial cells - by Bioz Stars,
2025-02
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ATCC
dermal microvascular endothelial cell ![]() Dermal Microvascular Endothelial Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/dermal microvascular endothelial cell/product/ATCC Average 86 stars, based on 1 article reviews
dermal microvascular endothelial cell - by Bioz Stars,
2025-02
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Lonza
primary human dermal microvascular endothelial cells hmvec d ![]() Primary Human Dermal Microvascular Endothelial Cells Hmvec D, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/primary human dermal microvascular endothelial cells hmvec d/product/Lonza Average 86 stars, based on 1 article reviews
primary human dermal microvascular endothelial cells hmvec d - by Bioz Stars,
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PromoCell
human dermal lymphatic microvascular endothelial cells ![]() Human Dermal Lymphatic Microvascular Endothelial Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/human dermal lymphatic microvascular endothelial cells/product/PromoCell Average 86 stars, based on 1 article reviews
human dermal lymphatic microvascular endothelial cells - by Bioz Stars,
2025-02
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ATCC
dermal microvascular endothelial cell line ![]() Dermal Microvascular Endothelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/dermal microvascular endothelial cell line/product/ATCC Average 86 stars, based on 1 article reviews
dermal microvascular endothelial cell line - by Bioz Stars,
2025-02
86/100 stars
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Lonza
human dermal microvascular endothelial cells hdmecs ![]() Human Dermal Microvascular Endothelial Cells Hdmecs, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/human dermal microvascular endothelial cells hdmecs/product/Lonza Average 86 stars, based on 1 article reviews
human dermal microvascular endothelial cells hdmecs - by Bioz Stars,
2025-02
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Journal: Angiogenesis
Article Title: Clioquinol inhibits angiogenesis by promoting VEGFR2 degradation and synergizes with AKT inhibition to suppress triple-negative breast cancer vascularization
doi: 10.1007/s10456-024-09965-1
Figure Lengend Snippet: Clioquinol selectively targets ECs and inhibits angiogenesis. a Viability (% of 0 µM) of HUVECs, HDMECs, hPC-PLs, NHDFs, MDA-MB-231, MCF-7, and 4T1-Luc2 cells after 48-hour exposure to a serial dilution of clioquinol, as assessed by WST-1 assay ( n = 4). b Cytotoxicity (% of total cell death) of clioquinol against HUVECs after 24-hour treatment, as assessed by LDH assay ( n = 4). c Proliferation (% of 0 µM) of HUVECs treated with 0, 2.5, 5, or 10 µM clioquinol for 24 h, as assessed by BrdU incorporation assay ( n = 4). d Light microscopic images of migrated HUVECs after 5-hour incubation. The cells were treated with 0, 2.5, 5, or 10 µM clioquinol for 24 h prior to this assay. Scale bar: 65 μm. e Migration (% of 0 µM) of HUVECs treated as described in (d) ( n = 3). f Phase-contrast microscopic images of tube-forming HUVECs after 18-hour treatment with 0, 2.5, 5, or 10 µM clioquinol. Scale bar: 700 μm. g Tube formation (% of 0 µM) of HUVECs treated as described in (f) ( n = 3). h Phase-contrast microscopic images of HUVEC spheroids after 24-hour treatment with 0, 2.5, 5, or 10 µM clioquinol. Scale bar: 95 μm. i Sprouting (% of 0 µM) of HUVEC spheroids treated as described in (h) ( n = 11–13). j Fluorescence microscopic images of Matrigel plugs containing DMSO (control) or 10 µM clioquinol. The sections were stained with an anti-CD31 antibody (red) and Hoechst 33342 (blue) for the visualization of ECs and cell nuclei, respectively. Scale bar: 45 μm. k Microvessel density (% of control) of control and clioquinol-containing Matrigel plugs, as assessed by immunohistochemistry ( n = 7). Means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. (a-c, e, g, i: one-way ANOVA with Tukey’s multiple comparisons test; k: unpaired Student’s t-test)
Article Snippet: Human umbilical vein endothelial cells (HUVECs),
Techniques: Serial Dilution, WST-1 Assay, Lactate Dehydrogenase Assay, BrdU Incorporation Assay, Incubation, Migration, Fluorescence, Control, Staining, Immunohistochemistry
Journal: Angiogenesis
Article Title: Clioquinol inhibits angiogenesis by promoting VEGFR2 degradation and synergizes with AKT inhibition to suppress triple-negative breast cancer vascularization
doi: 10.1007/s10456-024-09965-1
Figure Lengend Snippet: Clioquinol selectively down-regulates VEGFR2 in ECs. a Western blots showing VEGFR2, VEGFR1, Tie2, FGFR1, and β-actin expression in HUVECs after 4-hour treatment with 0, 2.5, 5, or 10 µM clioquinol. b - e Expression level (% of 0 µM) of VEGFR2 ( b ), VEGFR1 ( c ), Tie2 ( d ), and FGFR1 ( e ) normalized to β-actin in HUVECs treated as described in (a) ( n = 3 independent experiments). f Mean fluorescence intensity (MFI) of membrane VEGFR2 on HUVECs treated with or without 10 µM clioquinol for 0.5, 1, 2, 3, and 4 h, as assessed by flow cytometry ( n = 4). g Phase-contrast microscopic images of HUVEC spheroids after 24-hour treatment without or with clioquinol in the absence or presence of 25 ng/mL VEGF. Scale bar: 135 μm. h Sprouting (% of control) of HUVEC spheroids treated as described in (g) ( n = 13–15). i Western blots showing VEGFR2 and β-actin expression in HUVECs, HDMECs, hPC-PLs, NHDFs, MCF-7, MDA-MB-231, and 4T1-Luc2 cells. j Expression level (% of HUVEC) of VEGFR2 normalized to β-actin in different cell types as described in (i) ( n = 3 independent experiments). k Correlation between cell viability and VEGFR2 expression following exposure to 10 or 25 µM clioquinol. Means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. (b-e, h, j: one-way ANOVA with Tukey’s multiple comparisons test; f: unpaired Student’s t-test; k: Pearson correlation coefficient)
Article Snippet: Human umbilical vein endothelial cells (HUVECs),
Techniques: Western Blot, Expressing, Fluorescence, Membrane, Flow Cytometry, Control
Journal: bioRxiv
Article Title: Telomerase mRNA Reduces Radiation-induced DNA Damage of human skin
doi: 10.1101/2025.02.01.636031
Figure Lengend Snippet: ( A-B ) Long amplicon PCR (LA-PCR) analysis of genomic and mitochondrial DNA integrity in human epidermal keratinocytes, dermal fibroblasts, and dermal microvascular endothelial cells immediately after exposure to different doses of X-ray irradiation (2 Gy, 5 Gy, 10 Gy, 20 Gy) or no irradiation (No IR, control). ( A ) Genomic DNA integrity was assessed using a 10.4 kb long amplicon from the HPRT gene and a 0.2 kb short amplicon from the same gene as a loading control. ( B ) Mitochondrial DNA integrity was assessed using an 8.8 kb long amplicon from the mitochondrial genome and a 0.2 kb short amplicon as a loading control. PCR products were resolved on agarose gels (0.8% for long amplicons; 2% for short amplicons). Representative gel images from three independent experiments are shown (top panels). Quantification of LA-PCR results normalized to the short amplicon controls is shown (bottom panels). ( C ) Quantification of cell death in human keratinocytes by flow cytometry at 2 hours and 24 hours post-exposure to the indicated doses of X-ray irradiation. ( D-E ) Representative flow cytometry plots and quantitative analysis of early and late apoptosis in human keratinocytes at 2 hours and 24 hours post-irradiation. Data are presented as mean ± SD (n = 3).
Article Snippet:
Techniques: Amplification, Irradiation, Control, Flow Cytometry
Journal: bioRxiv
Article Title: Telomerase mRNA Reduces Radiation-induced DNA Damage of human skin
doi: 10.1101/2025.02.01.636031
Figure Lengend Snippet: ( A ) Human epidermal fibroblast and keratinocytes were treated with vehicle control or GFP mRNA encapsulated by cationic lipid nanoparticle DOTAP or mixed with commercial transfection reagents (Lipofectamine Max or jetMessenger) to assess transfection efficiency. Representative images of green fluorescence from live cells (left panel) and DAPI-stained nuclei are shown (n = 3 independent experiments). Quantification GFP+ cells/DAPI are presented as mean percentage ± SD (n = 3). ( B ) Schematic outline of the experimental setup. ( C-E ) Representative flow cytometry plots and quantitative analysis of early and late apoptosis in human epidermal keratinocytes, assessed 24 hours after ionizing radiation (5 Gy) or no irradiation (No IR control). ( F, G ) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in indicated groups of human epidermal keratinocytes. ( H, I ) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in human dermal microvascular endothelial cells treated with indicated groups. Primary cells were transfected with DOTAP-encapsulated GFP or hTERT mRNA or treated with vehicle control. Flow cytometry analysis was performed 24 hours following 5 Gy irradiation or No IR control. Early apoptosis was identified as Zombie Aqua-negative and Annexin V-positive cells, while late apoptosis was identified as Zombie Aqua-positive and Annexin V-positive cells. Data are shown as mean ± SD (n = 3). ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001, ****, P < 0.0001. P values were calculated using two-way ANOVA. KTN: Epidermal Keratinocytes; DMEC: Dermal Microvascular Endothelial Cells.
Article Snippet:
Techniques: Control, Transfection, Fluorescence, Staining, Flow Cytometry, Irradiation
Journal: bioRxiv
Article Title: Telomerase mRNA Reduces Radiation-induced DNA Damage of human skin
doi: 10.1101/2025.02.01.636031
Figure Lengend Snippet: ( A-C ) Representative flow cytometry plots and quantitative analysis of early and late apoptosis in human dermal microvascular endothelial cells, assessed 24 hours after ionizing radiation (5 Gy) or no irradiation (No IR control). ( D, E ) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in indicated groups of human fibroblasts. Data are shown as mean ± SD (n = 3). ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001. P values were calculated using two-way ANOVA. DMEC: Dermal Microvascular Endothelial Cells; DF: Dermal Fibroblasts.
Article Snippet:
Techniques: Flow Cytometry, Irradiation, Control
Journal: bioRxiv
Article Title: Telomerase mRNA Reduces Radiation-induced DNA Damage of human skin
doi: 10.1101/2025.02.01.636031
Figure Lengend Snippet: (A-C ) Quantification of DNA damage by long amplicon PCR (LA-PCR) analysis of genomic DNA isolated from human epidermal keratinocytes, dermal fibroblasts, and dermal microvascular endothelial cells exposed to 5 Gy irradiation or no irradiation. At 0 h, 0.5 h, 1 h, 2 h, 6 h, and 24 h post-irradiation, DNA was analyzed using primers for a long amplicon (10.4 kb region in the HPRT gene) and a short amplicon (0.2 kb region in the HPRT gene) as a loading control. PCR products were resolved on agarose gels (0.8% for long amplicons, 2% for short amplicons). Representative gel images from three independent experiments are shown (top panels), with quantification of genomic DNA integrity normalized to the short amplicon control (bottom panels). ( D ) Representative images of MitoSOX analysis at 24 hours post-irradiation in keratinocytes treated with vehicle control, GFP mRNA, or TERT mRNA. ( E-F ) Representative flow cytometry plots and quantitative analysis of MitoSOX Red fluorescence in keratinocytes treated with vehicle, GFP mRNA, or TERT mRNA 24 hours after irradiation, indicating differences in mitochondrial superoxide production. MFI: Mean Fluorescence Intensity.
Article Snippet:
Techniques: Amplification, Isolation, Irradiation, Control, Flow Cytometry, Fluorescence
Journal: Microbiology Spectrum
Article Title: Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1
doi: 10.1128/spectrum.02221-24
Figure Lengend Snippet: Transendothelial migration of B. burgdorferi in Transwell chambers. (A) To study B. burgdorferi transmigration, Transwell chambers were seeded with hMVEC-d or hTERT cells until a tight monolayer was formed (<4% albumin diffusion). The upper chamber was infected with 3 × 10 5 spirochetes; after 20 h of infection, we counted the spirochetes in both the upper and lower chambers (either manually or by flow cytometry) and calculated the percentage of total transmigrated spirochetes (lower chamber). ( B, C ) The graphs show the percentage (mean ± SD) of B. burgdorferi that had transmigrated through human microvascular endothelial cells as determined by counting spirochetes in both the upper and lower chamber by flow cytometry. The data represent the mean of % transmigration ±SD of three independent experiments performed in quadruplicate and analyzed for significance using the Mann–Whitney test. NI denotes the non-infectious strain GCB705 (B31-A + pTM61 gent,gfp, see ), and WT indicates GCB726. ( D ) Evaluation of B. burgdorferi transendothelial migration in hTERT treated with AKB-9785. The complete chamber was treated with 5 µM AKB9785 for the duration of the assay. The upper chamber was infected with 3 × 10 5 spirochetes, and the percentage of total transmigrated spirochete was assessed by counting in a Petroff–Hausser chamber and dark-field microscopy after 20 h of infection. The data represent the mean % transmigration ±SD of three independent experiments performed in quadruplicate; non-parametric ANOVA was performed to compare the control cells with the treated cells using the Kruskal–Wallis post-test (ns = not significant). ( E ) Demonstration of the effectiveness of AKB-9785 to lock intercellular junctions in hTERT cells. Ly 294002 at 40 µM was used to disrupt the monolayer, and AKB-9785 was used to lock intercellular junctions and preserve monolayer integrity, which was assessed using an albumin diffusion assay: 10 μg of 555-Alb was added to the upper chamber at 16 h, and the reading was carried out at the final point (20 h). The graph represents the mean ± SD of three experiments, with 1–4 samples for each experimental condition. Statistics were evaluated with the Kruskal–Wallis test and Dunn’s multiple comparison; P < 0.05 was considered significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns = not significant.
Article Snippet: Primary human dermal microvascular endothelial cells (hMVEC-d) were purchased from Lonza (CC-2543), grown in Basal Medium (EBM-2) (CC-3156, Lonza) complete media (with supplements EGMTM-2 SingleQuotsTM Supplements [CC-4176, Lonza]) at 37°C under 5% CO 2 and used before passage five. hTERT-immortalized
Techniques: Migration, Transmigration Assay, Diffusion-based Assay, Infection, Flow Cytometry, MANN-WHITNEY, Microscopy, Control, Comparison
Journal: Microbiology Spectrum
Article Title: Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1
doi: 10.1128/spectrum.02221-24
Figure Lengend Snippet: Transendothelial migration of B. burgdorferi in Transwell chambers. (A) To study B. burgdorferi transmigration, Transwell chambers were seeded with hMVEC-d or hTERT cells until a tight monolayer was formed (<4% albumin diffusion). The upper chamber was infected with 3 × 10 5 spirochetes; after 20 h of infection, we counted the spirochetes in both the upper and lower chambers (either manually or by flow cytometry) and calculated the percentage of total transmigrated spirochetes (lower chamber). ( B, C ) The graphs show the percentage (mean ± SD) of B. burgdorferi that had transmigrated through human microvascular endothelial cells as determined by counting spirochetes in both the upper and lower chamber by flow cytometry. The data represent the mean of % transmigration ±SD of three independent experiments performed in quadruplicate and analyzed for significance using the Mann–Whitney test. NI denotes the non-infectious strain GCB705 (B31-A + pTM61 gent,gfp, see ), and WT indicates GCB726. ( D ) Evaluation of B. burgdorferi transendothelial migration in hTERT treated with AKB-9785. The complete chamber was treated with 5 µM AKB9785 for the duration of the assay. The upper chamber was infected with 3 × 10 5 spirochetes, and the percentage of total transmigrated spirochete was assessed by counting in a Petroff–Hausser chamber and dark-field microscopy after 20 h of infection. The data represent the mean % transmigration ±SD of three independent experiments performed in quadruplicate; non-parametric ANOVA was performed to compare the control cells with the treated cells using the Kruskal–Wallis post-test (ns = not significant). ( E ) Demonstration of the effectiveness of AKB-9785 to lock intercellular junctions in hTERT cells. Ly 294002 at 40 µM was used to disrupt the monolayer, and AKB-9785 was used to lock intercellular junctions and preserve monolayer integrity, which was assessed using an albumin diffusion assay: 10 μg of 555-Alb was added to the upper chamber at 16 h, and the reading was carried out at the final point (20 h). The graph represents the mean ± SD of three experiments, with 1–4 samples for each experimental condition. Statistics were evaluated with the Kruskal–Wallis test and Dunn’s multiple comparison; P < 0.05 was considered significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns = not significant.
Article Snippet:
Techniques: Migration, Transmigration Assay, Diffusion-based Assay, Infection, Flow Cytometry, MANN-WHITNEY, Microscopy, Control, Comparison
Journal: Cancers
Article Title: Impact of Hyaluronic Acid on the Cutaneous T-Cell Lymphoma Microenvironment: A Novel Anti-Tumor Mechanism of Bexarotene
doi: 10.3390/cancers17020324
Figure Lengend Snippet: HA affects tumor cell proliferation and the CTCL microenvironment. ( A ) Proliferation of EL4 and HuT78 cells in vitro in the presence of LMWHA and HMWHA. The left graph shows the number of live EL4 and HuT78 cells (n = 4 each). The right panel shows the results of MTT proliferation assays (n = 6 each). The MTT results refer to cells measured five days after treatment. Asterisks indicate p < 0.05 in comparing LMWHA and HMWHA. ( B ) Change in tumor volume under LMWHA stimulation in vivo. The asterisk indicates p < 0.05. Representative images of tumor size at day 10 are shown. ( C ) Expression of Th1 and Th2 chemokines by M2 macrophages with HA stimulation (n = 4 each). ( D ) Tube formation assay performed with HDMECs treated with LMWHA or HMWHA. Representative images are shown (n = 5 each). Bar = 300 µm. AU, arbitrary unit; NT, no treatment; LMW, low-molecular-weight; HMW, high-molecular-weight.
Article Snippet:
Techniques: In Vitro, In Vivo, Expressing, Tube Formation Assay, Molecular Weight