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dermal cell line mouse nih  (ATCC)


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    ATCC dermal cell line mouse nih
    Dermal Cell Line Mouse Nih, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dermal cell line mouse nih/product/ATCC
    Average 95 stars, based on 1 article reviews
    dermal cell line mouse nih - by Bioz Stars, 2025-02
    95/100 stars

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    95
    ATCC dermal cell line mouse nih
    Dermal Cell Line Mouse Nih, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dermal cell line mouse nih/product/ATCC
    Average 95 stars, based on 1 article reviews
    dermal cell line mouse nih - by Bioz Stars, 2025-02
    95/100 stars
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    95
    ATCC mouse dermal fibroblast cell line nih3t3
    Induction of TAMs and CAFs by IL-6 in human bladder cancer tissues and confirmation by in vitro experiments. (A) Representative expression status for IL-6 in human bladder cancer tissues. Images were captured at 200× magnification. (B) The interrelationship between the percentage of IL-6-positive cancer cells and the number of tumor-infiltrating TAMs was examined using Spearman’s correlation. Spearman r was found to be 0.68 (95% confidence interval, 058–0.76). (C) Comparison of the percentage of IL-6-positive cancer with the induction level of CAFs (CAF score). (D) Morphological changes of THP-1 cells by PMA and IL-4 treatment. THP-1 cells were treated with 200 nM PMA for 24 h to differentiate them into resting macrophages (middle), followed by treatment with IL-4 (20 ng/mL) for 48 h (right). (E) Western blot analysis for confirming the generation of TAMs and CAFs. THP-1 cells were treated with a combination of PMA and 20 ng/mL of CXCL1, IL-6, or TGF-β, separately. Upregulation of CD204 indicates differentiation into TAMs. <t>NIH3T3</t> cells were treated with 20 ng/mL of CXCL1, IL-6, or TGF-β, separately. Upregulation of αSMA indicates the activation of fibroblasts, which are known to be CAFs.
    Mouse Dermal Fibroblast Cell Line Nih3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse dermal fibroblast cell line nih3t3/product/ATCC
    Average 95 stars, based on 1 article reviews
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    Induction of TAMs and CAFs by IL-6 in human bladder cancer tissues and confirmation by in vitro experiments. (A) Representative expression status for IL-6 in human bladder cancer tissues. Images were captured at 200× magnification. (B) The interrelationship between the percentage of IL-6-positive cancer cells and the number of tumor-infiltrating TAMs was examined using Spearman’s correlation. Spearman r was found to be 0.68 (95% confidence interval, 058–0.76). (C) Comparison of the percentage of IL-6-positive cancer with the induction level of CAFs (CAF score). (D) Morphological changes of THP-1 cells by PMA and IL-4 treatment. THP-1 cells were treated with 200 nM PMA for 24 h to differentiate them into resting macrophages (middle), followed by treatment with IL-4 (20 ng/mL) for 48 h (right). (E) Western blot analysis for confirming the generation of TAMs and CAFs. THP-1 cells were treated with a combination of PMA and 20 ng/mL of CXCL1, IL-6, or TGF-β, separately. Upregulation of CD204 indicates differentiation into TAMs. NIH3T3 cells were treated with 20 ng/mL of CXCL1, IL-6, or TGF-β, separately. Upregulation of αSMA indicates the activation of fibroblasts, which are known to be CAFs.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: CXCL1-Mediated Interaction of Cancer Cells with Tumor-Associated Macrophages and Cancer-Associated Fibroblasts Promotes Tumor Progression in Human Bladder Cancer

    doi: 10.1016/j.neo.2016.08.002

    Figure Lengend Snippet: Induction of TAMs and CAFs by IL-6 in human bladder cancer tissues and confirmation by in vitro experiments. (A) Representative expression status for IL-6 in human bladder cancer tissues. Images were captured at 200× magnification. (B) The interrelationship between the percentage of IL-6-positive cancer cells and the number of tumor-infiltrating TAMs was examined using Spearman’s correlation. Spearman r was found to be 0.68 (95% confidence interval, 058–0.76). (C) Comparison of the percentage of IL-6-positive cancer with the induction level of CAFs (CAF score). (D) Morphological changes of THP-1 cells by PMA and IL-4 treatment. THP-1 cells were treated with 200 nM PMA for 24 h to differentiate them into resting macrophages (middle), followed by treatment with IL-4 (20 ng/mL) for 48 h (right). (E) Western blot analysis for confirming the generation of TAMs and CAFs. THP-1 cells were treated with a combination of PMA and 20 ng/mL of CXCL1, IL-6, or TGF-β, separately. Upregulation of CD204 indicates differentiation into TAMs. NIH3T3 cells were treated with 20 ng/mL of CXCL1, IL-6, or TGF-β, separately. Upregulation of αSMA indicates the activation of fibroblasts, which are known to be CAFs.

    Article Snippet: Four bladder cancer cell lines, J82, UMUC3, T24 (ATCC, Manassas, VA, USA), MGH-U3 (a generous gift from Dr H. LaRue at Laval University Cancer Research Centre, Quebec, Canada), benign bladder cell line UROtsa (a generous gift from Dr Donald Sens at the University of North Dakota School of Medicine, Grand Forks, ND, USA), acute monocytic leukemia cell line THP-1 (ATCC), mouse dermal fibroblast cell line NIH3T3 (ATCC), and HEK293FT (Invitrogen, Carlsbad, CA, USA) were used in the present study.

    Techniques: In Vitro, Expressing, Western Blot, Activation Assay

    Association of CXCL1 production in TAMs/CAFs with invasion potential of human bladder cancer. (A) Dual immunofluorescence staining analysis for CD204 or αSMA (green) and CXCL1 (red) shows the expression of CXCL1 in TAMs and CAFs in the tumoral area. Overlay images and their magnified images are shown (2 panels on the right). Original magnification, 200×. (B) CXCL1 mRNA expression level was determined by real-time RT-PCR. Expression levels are the result of 3 experiments and are expressed as the mean ± SD relative to that of UROtsa, which is defined as 1. (C) THP-1 and NIH3T3 cells were infected with lentiviral constructs harboring empty vectors (LvNega) or CXCL1 expression vectors (LvCXCL1). Western blot analysis demonstrated altered CXCL1 expression in both cell lines. The changes in the expression levels of CD204 and αSMA were evaluated in infected THP-1 and NIH3T3 cells, respectively. Actin served as the loading control. (D) MGH-U3 and T24 cells were suspended in the conditioned media collected from manipulated TAMs and CAFs and subjected to in vitro invasion assays. Data are expressed as the mean ± SD of 3 independent experiments conducted in triplicate; *, P < 0.05, **, and P < 0.01.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: CXCL1-Mediated Interaction of Cancer Cells with Tumor-Associated Macrophages and Cancer-Associated Fibroblasts Promotes Tumor Progression in Human Bladder Cancer

    doi: 10.1016/j.neo.2016.08.002

    Figure Lengend Snippet: Association of CXCL1 production in TAMs/CAFs with invasion potential of human bladder cancer. (A) Dual immunofluorescence staining analysis for CD204 or αSMA (green) and CXCL1 (red) shows the expression of CXCL1 in TAMs and CAFs in the tumoral area. Overlay images and their magnified images are shown (2 panels on the right). Original magnification, 200×. (B) CXCL1 mRNA expression level was determined by real-time RT-PCR. Expression levels are the result of 3 experiments and are expressed as the mean ± SD relative to that of UROtsa, which is defined as 1. (C) THP-1 and NIH3T3 cells were infected with lentiviral constructs harboring empty vectors (LvNega) or CXCL1 expression vectors (LvCXCL1). Western blot analysis demonstrated altered CXCL1 expression in both cell lines. The changes in the expression levels of CD204 and αSMA were evaluated in infected THP-1 and NIH3T3 cells, respectively. Actin served as the loading control. (D) MGH-U3 and T24 cells were suspended in the conditioned media collected from manipulated TAMs and CAFs and subjected to in vitro invasion assays. Data are expressed as the mean ± SD of 3 independent experiments conducted in triplicate; *, P < 0.05, **, and P < 0.01.

    Article Snippet: Four bladder cancer cell lines, J82, UMUC3, T24 (ATCC, Manassas, VA, USA), MGH-U3 (a generous gift from Dr H. LaRue at Laval University Cancer Research Centre, Quebec, Canada), benign bladder cell line UROtsa (a generous gift from Dr Donald Sens at the University of North Dakota School of Medicine, Grand Forks, ND, USA), acute monocytic leukemia cell line THP-1 (ATCC), mouse dermal fibroblast cell line NIH3T3 (ATCC), and HEK293FT (Invitrogen, Carlsbad, CA, USA) were used in the present study.

    Techniques: Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Infection, Construct, Western Blot, In Vitro

    Association of CXCL1 production in TAMs/CAFs with strong adhesion to bladder cancer cells and formation of homogenous multicellular spheroids. Bladder cancer cells T24 and MGH-U3 were labeled with PKH67 (green dye). THP-1-derived TAMs and NIH3T3-drived CAFs were labeled with PKH26 (red dye). After labeling, cancer cells and stromal cells were mixed at the same number (5×10 3 cells/well) and plated into a 3D culture plate. Photographs of randomly selected spheroids were captured 3 days after plating. Representative spheroids are shown as follows: (A) spheroids of the co-culture of T24 cells and TAMs; (B), spheroids of T24 cells and TAMs treated with anti-CXCL1-neutralizing antibody; C, spheroids of the co-culture of MGH-U3 cells and CAFs; (D) spheroids of MGH-U3 cells and CAFs treated with anti-CXCL1-neutralizing antibody. Original magnification, 400×.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: CXCL1-Mediated Interaction of Cancer Cells with Tumor-Associated Macrophages and Cancer-Associated Fibroblasts Promotes Tumor Progression in Human Bladder Cancer

    doi: 10.1016/j.neo.2016.08.002

    Figure Lengend Snippet: Association of CXCL1 production in TAMs/CAFs with strong adhesion to bladder cancer cells and formation of homogenous multicellular spheroids. Bladder cancer cells T24 and MGH-U3 were labeled with PKH67 (green dye). THP-1-derived TAMs and NIH3T3-drived CAFs were labeled with PKH26 (red dye). After labeling, cancer cells and stromal cells were mixed at the same number (5×10 3 cells/well) and plated into a 3D culture plate. Photographs of randomly selected spheroids were captured 3 days after plating. Representative spheroids are shown as follows: (A) spheroids of the co-culture of T24 cells and TAMs; (B), spheroids of T24 cells and TAMs treated with anti-CXCL1-neutralizing antibody; C, spheroids of the co-culture of MGH-U3 cells and CAFs; (D) spheroids of MGH-U3 cells and CAFs treated with anti-CXCL1-neutralizing antibody. Original magnification, 400×.

    Article Snippet: Four bladder cancer cell lines, J82, UMUC3, T24 (ATCC, Manassas, VA, USA), MGH-U3 (a generous gift from Dr H. LaRue at Laval University Cancer Research Centre, Quebec, Canada), benign bladder cell line UROtsa (a generous gift from Dr Donald Sens at the University of North Dakota School of Medicine, Grand Forks, ND, USA), acute monocytic leukemia cell line THP-1 (ATCC), mouse dermal fibroblast cell line NIH3T3 (ATCC), and HEK293FT (Invitrogen, Carlsbad, CA, USA) were used in the present study.

    Techniques: Labeling, Derivative Assay, Co-Culture Assay